Animal shelters are an extremely stressful environment for a dog, most specifically due to social isolation and novel surroundings. The stress response of dogs housed in this environment may be ...alleviated through human interaction shortly after arrival. During their second day in a public animal shelter, adult stray dogs were either engaged in a human contact session or not. The session involved taking the dog into an outdoor enclosure, playing with the dog, grooming, petting and reviewing basic obedience commands. Each dog interacted with a human for approximately 45 min. Salivary cortisol levels were examined from each dog on their 2nd, 3rd, 4th and 9th day of housing. Animals that engaged in a human contact session had lower cortisol levels on day 3 than animals that did not. Breed type, sex and age did not have an effect on cortisol levels on any day measured. A human interaction session can be beneficial to both animal welfare and adoption procedures. The current study not only utilized the human contact session as a treatment to reduce stress but also as a resource for individual temperament/personality information that could be later used to facilitate compatible adoptions. Human interaction may be an effective means of reducing the cortisol response of dogs in the aversive shelter environment.
Abstract
Pulmonary hypertension is a noninfectious disease of cattle at altitudes > 1524 m (5,000 ft). Mean pulmonary arterial pressures (PAP) are used as an indicator for pulmonary hypertension in ...cattle. High PAP cattle (≥50 mmHg) entering the feedlot at moderate elevations have lower feed efficiency as compared to low PAP cattle (< 50 mmHg). The impact of pulmonary arterial pressure on mitochondrial function, oxidative phosphorylation (OXPHOS) protein abundance, and meat color was examined using longissimus lumborum (LL) from high (98 ± 13 mmHg; n = 5) and low (41 ± 3 mmHg; n = 6) PAP fattened Angus steers (live weight of 588 ± 38 kg) during early postmortem period (2 and 48 h) and retail display (days 1 to 9), respectively. High PAP muscle had greater (P = 0.013) OXPHOS-linked respiration and proton leak-associated respiration than low PAP muscles at 2 h postmortem but rapidly declined to be similar (P = 0.145) to low PAP muscle by 48 h postmortem. OXPHOS protein expression was higher (P = 0.045) in low PAP than high PAP muscle. During retail display, redness, chroma, hue, ratio of reflectance at 630 and 580 nm, and metmyoglobin reducing activity decreased faster (P < 0.05) in high PAP steaks than low PAP. Lipid oxidation significantly increased (P < 0.05) in high PAP steaks but not (P > 0.05) in low PAP. The results indicated that high PAP caused a lower OXPHOS efficiency and greater fuel oxidation rates under conditions of low ATP demand in premortem beef LL muscle; this could explain the lower feed efficiency in high PAP feedlot cattle compared to low PAP counterparts. Mitochondrial integral function (membrane integrity or/and protein function) declined faster in high PAP than low PAP muscle at early postmortem. LL steaks from high PAP animals had lower color stability than those from the low PAP animals during simulated retail display, which could be partially attributed to the loss of muscle mitochondrial function at early postmortem by ROS damage in high PAP muscle.
Lay Summary
The impact of pulmonary arterial pressure (PAP) on mitochondrial function, oxidative phosphorylation protein abundance, and meat color was examined using longissimus lumborum (LL) from high (98 ± 13 mmHg) and low (41 ± 3 mmHg) PAP fattened Angus steers (live weight of 588 ± 38 kg) during early postmortem period (2 and 48 h) and retail display (days 1 to 9), respectively. The results indicated that high PAP caused a lower oxidative phosphorylation efficiency and greater fuel oxidation rates under conditions of positive energy balance in beef LL muscle. This could explain the lower feed efficiency in high PAP feedlot cattle compared to low PAP counterparts. Mitochondrial integral function declined faster in high PAP than low PAP muscle at early postmortem. LL steaks from high PAP animals had lower color stability than those from the low PAP animals during simulated retail display, which could be partially attributed to the loss of muscle mitochondrial function at early postmortem in high PAP muscle
High pulmonary arterial pressure (PAP) caused a lower oxidative phosphorylation efficiency and greater fuel oxidation rates under conditions of low ATP demand in beef longissimus lumborum(LL) muscle. Moreover, LL steaks from high PAP animals had lower color stability than those from the low PAP animals during simulated retail display, which could be partially attributed to the faster decline of mitochondrial integral function in high PAP than low PAP muscle at early postmortem.
Abstract
Pulmonary arterial pressure (PAP) can be used as an indicator of susceptibility to pulmonary hypertension and subsequent potential to develop right-sided heart failure (RHF). Previously ...reported heritability estimates of PAP have been moderate to high. Based on these estimates, selection for the indicator trait, PAP, could reduce the incidence of RHF due to hypoxia. Previous studies have also speculated that increased growth rates and body fat accumulation contribute to increased PAP and RHF. Research evaluating the genetic relationships between PAP and performance traits (e.g., yearling weight and postweaning gain) has yielded conflicting results, leading to ambiguity and uncertainty regarding the underlying genetic relationships. Additionally, no previous research has evaluated the relationship between PAP and ultrasound carcass traits. Therefore, the objective of this study was to estimate trait heritabilities and genetic correlations between PAP, post-weaning growth traits, and ultrasound carcass traits in Angus cattle, using data (n = 4,511) from the American Angus Association. We hypothesized that traits associated with increased growth and muscle would have a positive genetic (i.e., unfavorable) relationship with PAP. Estimates for heritability and genetic correlations were obtained using a multi-trait animal model. Heritability estimates for PAP (0.21 ± 0.04), post-weaning gain (PWG; 0.31 ± 0.04), and yearling weight (YWT; 0.37 ± 0.04) were within the range of estimates previously reported. Genetic correlations were weak (< 0.20) between PAP, PWG, and YWT. A low-to-moderate genetic correlation between PAP and ultrasound ribeye area (UREA) was found (0.25 ± 0.12). Genetic correlations between PAP, ultrasound back fat (UBF), ultrasound intramuscular fat (IMF), and ultrasound rump fat (RUMP) were weak (ranging in magnitude from −0.05 to 0.10) and therefore, do not provide strong support for the hypothesis of an antagonistic relationship between PAP and carcass ultrasound traits, while heritability estimates for UBF (0.43 ± 0.05), UREA (0.31 ± 0.04), IMF (0.35 ± 0.04), and RUMP (0.47 ± 0.05) were in the range of previously reported values.
The relationships of pulmonary arterial pressure with growth and carcass traits are relatively small excepting ribeye area where a moderate, unfavorable genetic relationship exists. Therefore, selection decisions should consider pulmonary arterial pressure in a multi-trait approach to beef cattle genetic improvement.
Lay Summary
Beef cattle residing >1,500 m are subject to reduced atmospheric oxygen levels when compared with beef cattle at lower elevations which may result in hypoxia-induced pulmonary hypertension and right-side heart failure. Breeders use pulmonary arterial pressures to identify animals at risk of right-side heart failure and to select breeding animals that are less susceptible to the problem. There is a concern that selection for growth and carcass characteristics may be increasing the incidence of heart failure in feedlot cattle at elevations <1,500 m. To address the concern, this study estimated the genetic correlations (i.e., relationships) between pulmonary arterial pressure, growth, and ultrasound carcass measures in Angus cattle. The study shows that the genetic relationships between pulmonary arterial pressure and these traits are minimal to non-existent with the exception of ultrasound ribeye area. Our estimate suggests a moderate, unfavorable relationship between pulmonary arterial pressure and ribeye area and that single-trait selection for increased ribeye area alone may result in increased susceptibility to right-side heart failure. However, selection for both traits simultaneously should overcome increases in susceptibility given the moderate strength of the genetic relationship.
Abstract
Heifer pregnancy (HPG) and Stayability (STAY) are female reproductive traits that have EPD reported by the Red Angus Association of America. Challenges arise when making genetic predictions ...for these traits. Specifically, HPG and STAY phenotypes can only be collected on females retained in the breeding herd and have low heritability estimates. Additionally, STAY is measured late in an animal’s life. The objective of this research was to investigate the genetic relationships between HPG or STAY and 13 other traits, which included measurements of growth, carcass, ultrasound, and scrotal circumference. For STAY relationships between mature weight (MW), body condition score (BCS), teat score (TS), and udder suspension score (US) were also evaluated. Data from 142,146 and 164,235 animals were used in the analyses for HPG and STAY, respectively. Genetic relationships were investigated using a series of 2 trait animal models and a REML procedure. In all analyses, the appropriate contemporary groups were included as a fixed effect, and direct genetic as a random effect. Additional fixed effects included as follows: sex for weight, carcass and ultrasound traits, age of dam for weight traits, and age of measurement for ultrasound, carcass, BCS, udder traits, and MW. Maternal genetic effects for preweaning gain (Pre-WG), weaning weight (WW), and yearling weight (YW) were also modeled. Permanent environmental effects of the dam were modeled for the traits Pre-WG and WW. Permanent environment of the individual for the traits MW, BCS, TS, and US was also included. Heritability estimates were 0.12 ± 0.01 and 0.10 ± 0.01 for HPG and STAY, respectively. Heritability estimates for direct genetic effects of production traits were moderate to high in magnitude, maternal heritability estimates were low, and permanent environmental effects accounted for 0.00 to 0.18 of the total variation. The strongest genetic correlations were those among Pre-WGD (0.24 ± 0.08), WWD (0.18 ± 0.08), YWD (0.20 ± 0.07), ultrasound rib eye area direct (0.16 ± 0.08), and ultrasound backfat direct (0.14 ± 0.08) and HPG. The highest genetic correlations were between STAY and WWM (0.54 ± 0.05), YWM (0.36 ± 0.07), backfat (0.53 ± 0.20), marbling score (0.40 ± 0.20), UREA (0.19 ± 0.07), ultrasound backfat (0.37 ± 0.07), TS (0.30 ± 0.11), and US (0.23 ± 0.11). Relationships between HPG or STAY and other traits were minimal. These results suggest that genetic relationships exist between HPG or STAY and other more densely recorded traits.
Abstract
Pulmonary arterial pressure (PAP) is a diagnostic measure used to determine an individual’s susceptibility to developing high-altitude disease. The importance of PAP measures collected at ...elevations lower than the intended breeding elevation of the bulls (i.e., ≥1,520 m) is unknown. Therefore, the objective of this study was to determine the genetic relationship between PAP measures collected in a range of elevations using reaction norm models. A total of 9,177 PAP and elevation observations on purebred Angus cattle, which averaged 43.49 ± 11.32 mmHg and 1,878.6 ± 296.8 m, respectively, were used in the evaluation. The average age of the individuals in the evaluation was 434.04 ± 115.9 d. A random regression model containing the effects of sex, a linear covariate of age, a quadratic fixed covariate of elevation, and random effects consisting of a contemporary group and a linear regression of PAP on elevation was used for the evaluation of PAP. Two forms of PAP were evaluated with this model. First, to address the non-normality of the data, PAP was raised to the power of −2.6 (ptPAP) based on the results of a Box–Cox analysis. Second, raw PAP (rPAP) phenotypes were evaluated to compare the results to those obtained from the transformed data. For ptPAP, heritability ranged from 0.25 to 0.37 corresponding to elevations of 1,900 and 1,215 m, respectively. For rPAP, heritability ranged from 0.22 to 0.41 corresponding to elevations of 1,700 and 2,495 m, respectively. Generally, lower elevations corresponded to decreased heritabilities while higher elevations corresponded to increased heritability estimates. For ptPAP, genetic correlations ranged from 0.18 (elevation: 1,215 and 2,495 m) to 1.00. For rPAP, genetic correlations ranged from 0.08 (elevation: 1,215 and 2,495 m) to 1.00. In general, the closer the elevations in which PAP was measured, the greater the genetic relationship. The greater the difference in elevation between PAP measures resulted in lower genetic correlations. The rank correlation between expected progeny differences (EPD) for 1,215 and 2,495 m was 0.65 and 0.49 for the ptPAP and rPAP, respectively. These results suggested that PAP measures collected in lower elevations may be used as an indicator of high-altitude adaptability. In the estimation of EPD to rank sires for their suitability for use in high-elevation production systems, it is important to account for the relationships among varied altitudes.
Abstract
Data on calving ease (CE) and birth, weaning weight (WW), and yearling weight (YW) were obtained from the American Simmental Association (ASA) and included pedigree and performance ...information on 11,640,735 animals. Our objective was to quantify differential response from selection for high CE vs. low birth weight (LBW) in first-calf Simmental heifers. We hypothesized that direct selection for CE should be used as the primary approach to reduce dystocia and mitigate losses in growth-related traits. WW and YW were adjusted to 205 and 365 d of age, respectively. Sire and maternal grandsire (co)variance components for CE, birth weight (BW), and 205-d weaning weight (205-d WW), and sire covariance components for 160-d postweaning gain (160-d gain) were estimated using a sire-maternal grandsire model. Direct and maternal expected progeny differences (EPD) for CE, BW, and 205-d WW and direct EPD for 160-d gain and 365-d yearling weight (365-d YW) for first-calf Simmental heifers population (465,710 animals) were estimated using a threshold-linear multivariate maternal animal model. This population was used to estimate genetic trends and as a selection pool (control) for various selection scenarios. Selection scenarios were high CE (HCE), LBW, the all-purpose selection index (API = −1.8 BW + 1.3 CE + 0.10 WW + 0.20 YW) of the ASA and its two derived subindices: (API1 = 1.3 CE + 0.20 YW) and (API2 = −1.8 BW + 0.20 YW), and lastly Dickerson’s selection index (DSI = −3.2 BW + YW). Data for each selection scenario were created by selecting sires with EPD greater than or equal to the average along with the top 75% of dams. Comparison between selection scenarios involved evaluating the direct and maternal genetic trends from these scenarios. Direct heritabilities for CE, BW, 205-d WW, 160-d gain, and 365-d YW of Simmental cattle were 0.23, 0.52, 0.28, 0.21, and 0.33, respectively. The single trait, HCE, selection scenario, as opposed to LBW, increased the intercept for CE by 57.7% and the slopes (P < 0.001) for BW, 205-d WW, 160-d gain, and 365-d YW by 27.9%, 37.5%, 16%, and 28%, respectively. Comparisons of various selection scenarios revealed that the CE-based selection scenarios (HCE, API, and API1) had a greater response for CE and growth traits.
Dairy production in Holstein cows in a semiarid environment is challenging due to heat stress. Under such conditions, genetic selection for heat tolerance appears to be a useful strategy. The ...objective was to validate molecular markers associated with milk production and thermotolerance traits in Holstein cows managed in a hot and humid environment. Lactating cows (
= 300) exposed to a heat stress environment were genotyped using a medium-density array including 53,218 SNPs. A genome-wide association study (GWAS) detected six SNPs associated with total milk yield (MY305) that surpassed multiple testing (
< 1.14 × 10
). These SNPs were further validated in 216 Holstein cows from two independent populations that were genotyped using the TaqMan bi-allelic discrimination method and qPCR. In these cows, only the SNPs rs8193046, rs43410971, and rs382039214, within the genes
,
, and
, respectively, were associated (
< 0.05) with MY305, rectal temperature (RT), and respiratory rate. Interestingly, these variables improved as the number of favorable genotypes of the SNPs increased from 0 to 3. In addition, a regression analysis detected RT as a significant predictor (R
= 0.362) for MY305 in cows with >1 favorable genotype, suggesting this close relationship was influenced by genetic markers. In conclusion, SNPs in the genes
,
, and
appear to be involved in the molecular mechanism that regulates milk production in cows under heat-stressed conditions. These SNPs are proposed as thermotolerance genetic markers for a selection program to improve the milk performance of lactating Holstein cows managed in a semiarid environment.
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