Immunohistochemistry is a method that can provide complementary diagnostic and prognostic information to morphological observations and soluble assays. Sensitivity, specificity, or requirements for ...arduous sample preparation or signal amplification procedures often limit the application of this approach to routine clinical specimens. Rolling circle amplification (RCA) generates a localized signal via an isothermal amplification of an oligonucleotide circle. The application of this approach to immunohistochemistry could extend the utility of these methods to include a more complete set of immunological and molecular probes. RCA-mediated signal amplification was successfully applied to the sensitive and specific detection of a variety of cell surface antigens (CD3, CD20, and epithelial membrane antigen) and intracellular molecules (vimentin and prostate-specific antigen) within a variety of routinely fixed specimens, as well as samples prepared for flow cytometry. RCA technology, which has an intrinsically wide dynamic range, is a robust and simple procedure that can provide a universal platform for the localization of a wide variety of molecules as a function of either antigenicity or nucleic acid sequence. The use of RCA in this way could enhance the use of markers of current interest as well as permit the integration of emerging information from genomics and proteomics into cell- and tissue-based analyses.
The research results of the supercapacitors influence on the voltage dip magnitude and duration in DC auxiliary power systems of substations were presented. The several variants of the ...supercapacitors' placement in DC network were discussed. The estimation of the fulfillment of the electromagnetic compatibility requirements with the consideration of voltage dips in DC network of substations during the short-circuit faults was made. The recommendations of the supercapacitors application for the enhancing of the operational reliability of substations' DC auxiliaries were proposed.
This article presents the means for reducing the capacity of batteries in DC auxiliary power systems of substations with electromagnetic drives of high-voltage switches. The evaluation of the ...reducing effect of supercapacitors on the capacity of the battery and the economic effect is given. The estimation of the fulfillment of requirements of voltage dips in DC network of substations during the electromagnetic drive starting was made. The recommendations of the supercapacitors application for using of batteries in DC auxiliary power systems of substations with lower capacity were proposed.
Rolling Circle Amplification Gusev, Yuriy; Sparkowski, Jason; Raghunathan, Arumugham ...
The American journal of pathology,
07/2001, Letnik:
159, Številka:
1
Journal Article
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Odprti dostop
Immunohistochemistry is a method that can provide complementary diagnostic and prognostic information to morphological observations and soluble assays. Sensitivity, specificity, or requirements for ...arduous sample preparation or signal amplification procedures often limit the application of this approach to routine clinical specimens. Rolling circle amplification (RCA) generates a localized signal via an isothermal amplification of an oligonucleotide circle. The application of this approach to immunohistochemistry could extend the utility of these methods to include a more complete set of immunological and molecular probes. RCA-mediated signal amplification was successfully applied to the sensitive and specific detection of a variety of cell surface antigens (CD3, CD20, and epithelial membrane antigen) and intracellular molecules (vimentin and prostate-specific antigen) within a variety of routinely fixed specimens, as well as samples prepared for flow cytometry. RCA technology, which has an intrinsically wide dynamic range, is a robust and simple procedure that can provide a universal platform for the localization of a wide variety of molecules as a function of either antigenicity or nucleic acid sequence. The use of RCA in this way could enhance the use of markers of current interest as well as permit the integration of emerging information from genomics and proteomics intocell- and tissue-based analyses.
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