We present a unique implementation of Python coding in an asynchronous object-oriented programming (OOP) framework to fully automate the process of collecting data with the George Mason University ...(GMU) Observatory's 0.8-meter telescope. The goal of this project is to perform automated follow-up observations for the Transiting Exoplanet Survey Satellite (TESS) mission, while still allowing for human control, monitoring, and adjustments. Prior to our implementation, the facility was computer-controlled by a human observer through a combination of webcams, TheSkyX, ASCOM Dome, MaxIm DL, and a weather station. We have automated slews and dome movements, CCD exposures, saving FITS images and metadata, initial focusing, guiding on the target, using the ambient temperature to adjust the focus as the telescope cools through the rest of the night, taking calibration images (darks and flats), and monitoring local weather data. The automated weather monitor periodically checks various weather data from multiple sources to automate the decision to close the observatory during adverse conditions. We have organized the OOP code structure in such a way that each hardware device or important higher-level process is categorized as its own object class or "module" with associated attributes and methods, with inherited common methods across modules for code reusability. To allow actions to be performed simultaneously across different modules, we implemented a multithreaded approach where each module is given its own CPU thread on which to operate concurrently with all other threads. After the initial few modules (camera, telescope, dome, data I/O) were developed, further development of the code was carried out in tandem with testing on sky on clear nights. The code, in its current state, has been tested and used for observations on 171 nights, with more planned usage and feature additions.
Officials in Iraq are eager to modernize the country's higher-education system after decades of isolation, but joint programs with universities in the West are slow to bear fruit.
VPS13 protein family members VPS13A through VPS13C have been associated with various recessive movement disorders. We describe the first disease association of rare recessive VPS13D variants ...including frameshift, missense, and partial duplication mutations with a novel complex, hyperkinetic neurological disorder. The clinical features include developmental delay, a childhood onset movement disorder (chorea, dystonia, or tremor), and progressive spastic ataxia or paraparesis. Characteristic brain magnetic resonance imaging shows basal ganglia or diffuse white matter T2 hyperintensities as seen in Leigh syndrome and choreoacanthocytosis. Muscle biopsy in 1 case showed mitochondrial aggregates and lipidosis, suggesting mitochondrial dysfunction. These findings underline the importance of the VPS13 complex in neurological diseases and a possible role in mitochondrial function. Ann Neurol 2018;83:1089–1095
A Monometallic Bis(cyaphido) Complex Levis, Madeleine C; Helm, Monte L; Turner, John F C ...
Chemistry : a European journal,
06/2024
Journal Article
Recenzirano
Odprti dostop
The first example of a bis(cyaphido) complex, trans-Ru(dppe)2(CºP)2, is described, unequivocally demonstrating the synthetic accessibility and stability of complexes that feature more than one ...cyaphido ligand. Synthesis is achieved from the precedent cation Ru(dppe)2(CΞP)+ via sequential coordination and desilylation of the phosphaalkyne Me3SiCΞP. The heteroleptic analogue trans-Ru(dppe)2(CΞN)(CΞP) is also prepared from the same cation and NaCN; both cyaphido complexes are structurally characterized, enabling the first direct comparison of cyaphide with cyanide, its isoelectronic and isolobal counterpart. This demonstrates an enhanced p-acidity for -CΞP over -CΞN, while computational studies reveal also a degree of p-donor character for the cyaphido ligand.
The role of posttranslational modifications in axonal injury and regeneration has been widely studied but there has been little consensus over the mechanism by which each modification affects adult ...axonal growth. Acetylation is known to play an important role in a variety of neuronal functions and its homeostasis is controlled by two enzyme families: the Histone Deacetylases (HDACs) and Histone Acetyl Transferases (HATs). Recent studies show that HDAC5 deacetylates microtubules in the axonal cytoplasm as part of an injury-induced regeneration response, but little is known about how acetylation of microtubules plays a role. Alpha-tubulin acetyl transferase (αTAT1) is a microtubule specific acetyl transferase that binds to microtubules and directly affects microtubule stability in cells. We hypothesize that increasing tubulin acetylation may play an important role in increasing the rate of axonal growth. In this study, we infected cultured adult DRG neurons with αTAT1 and αTAT1-D157N, a catalytically inactive mutant, and HDAC5, using lentiviruses. We found that αTAT1 significantly increases tubulin acetylation in 293T cells and DRG neurons but αTAT1-D157N does not. Furthermore, in neurons infected with αTAT1, a significant increase in acetylated tubulin was detected towards the distal portion of the axon but this increase was not detected in neurons infected with αTAT1-D157N. However, we found a significant increase in axon lengths of DRG neurons after αTAT1 and αTAT1-D157N infection, but no effect on axon lengths after infection with HDAC5. Our results suggest that while αTAT1 may play a role in axon growth in vitro, the increase is not directly due to acetylation of axonal microtubules. Our results also show that HDAC5 overexpression in the axonal cytoplasm does not play a crucial role in axonal regeneration of cultured DRG neurons. We expressed these genes in DRG neurons in adult rats and performed a sciatic nerve crush. We found that axons did not regenerate any better when infected with any of the constructs compared with control animals. Thus, while αTAT1 may be important for axonal growth in vitro, neither αTAT1 nor HDAC5 had an effect in vivo on the regeneration of sciatic nerves.
Direct electrophilic borylation using Y2BCl (Y2 = Cl2 or o-catecholato) with equimolar AlCl3 and a tertiary amine has been applied to a wide range of arenes and heteroarenes. In situ ...functionalization of the ArBCl2 products is possible with TMS2MIDA, to afford bench-stable and easily isolable MIDA-boronates in moderate to good yields. According to a combined experimental and computational study, the borylation of activated arenes at 20 °C proceeds through an SEAr mechanism with borenium cations, Y2B(amine)+, the key electrophiles. For catecholato-borocations, two amine dependent reaction pathways were identified: (i) With CatB(NEt3)+, an additional base is necessary to accomplish rapid borylation by deprotonation of the borylated arenium cation (σ complex), which otherwise would rather decompose to the starting materials than liberate the free amine to effect deprotonation. Apart from amines, the additional base may also be the arene itself when it is sufficiently basic (e.g., N-Me-indole). (ii) When the amine component of the borocation is less nucleophilic (e.g., 2,6-lutidine), no additional base is required due to more facile amine dissociation from the boron center in the borylated arenium cation intermediate. Borenium cations do not borylate poorly activated arenes (e.g., toluene) even at high temperatures; instead, the key electrophile in this case involves the product from interaction of AlCl3 with Y2BCl. When an extremely bulky amine is used, borylation again does not proceed via a borenium cation; instead, a number of mechanisms are feasible including via a boron electrophile generated by coordination of AlCl3 to Y2BCl, or by initial (heteroarene)AlCl3 adduct formation followed by deprotonation and transmetalation.
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