•Moringa oleifera Lam. seeds protein was converted to peptides by Alcalase hydrolyzed.•Peptides possess health-promoting activities.•Peptides could be as promising food additives for developing new ...functional food.
Moringa oleifera (MO) seeds treated as a new resource food rich in protein (37.48%), but, little know the bioactive benefits and peptide composition of protein hydrolysates. In this study, ultrafiltrated peptide fractions of MO seed proteins hydrolysates (MOSPH) were analyzed for their antioxidant, anti-hypertension and sleep-promoting activities. LC-MS/MS was used to identify the peptide composition of the molecular weight (MW) <3 kDa fraction. The results showed that MOSPH and the lower MW peptides fraction, especially the MW <3 kDa fraction, exhibited higher free radical scavenging and reducing capacity, in vitro angiotensin I-converting enzyme (ACE) inhibitory capacity and vasodilatory and anti-hypertensive effects on spontaneously hypertensive rats (SHR) after the development of hypertension, sleep-promoting and anticonvulsant effects in male ICR mice. Furthermore, four potential bioactive peptides (pepsite score > 0.3) from MW <3 kDa fraction of MOSPH were explored via in silico approach, of which a novel dual-function peptide IWHHTFYNELR (IR-11) exhibited higher ACE inhibitory activity (IC50 = 1.057 mg/mL) and DPPH•-clearing activity (IC50 = 0.404 mg/mL). Molecular docking indicates that IR-11 interacted with ACE and DPPH• through hydrogen bonding and hydrophobic forces. Our results suggested that MO seed proteins peptides can be a potent functional agents for developing new functional food.
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This study aimed to analyze metabolite abundances and proteome differences between buffalo milk from Binglangjiang (BBM) and Dehong (DBM). Untargeted UPLC-MS/MS, label-free proteomics approach, and ...bioinformatics analyses including GO, KEGG, and PPI were performed. The results revealed that catechol, L-proline, γ-aminobutyric acid, hippuric acid, creatinine, and betaine were detected in BBM and DBM as the major metabolites. One hundred thirteen proteins, mainly within the molecular weight range of 20-80 kDa, were identified, and the differential protein numbers of BBM were higher than those of DBM. These proteins were primarily involved in cholesterol metabolism, Staphylococcus aureus and Salmonella infections, the peroxisome proliferator-activated receptor signaling pathway, and coagulation cascades. Relative abundances of α-s1-casein, α-s2-casein, and β-casein were plentiful in BBM, while κ-casein, albumin, and α-lactalbumin were in DBM. PPI network analysis indicated that the identified proteins were highly interconnected. These findings promote a better understanding of quality biomarkers in dairy product improvements.
The prevalence of staphylococcal infection and the emergence of multidrug resistance of
Staphylococcus aureus
(
S. aureus
) are major concerns in food safety and public health. This study aimed to ...investigate the prevalence of
S. aureus
isolated from traditional Chinese
Rubing
and
Rushan
cheese, antimicrobial resistance profiles, genomic characteristics, and predict antimicrobial resistance genes (ARGs). From 124 samples, 18 of 62 (29.03%) of
Rubing
and 5 of 62 (8.06%) of
Rushan
cheese were confirmed to be
S. aureus
positive by standard culture-based methods. Twenty-three coagulase-positive staphylococci isolates were grouped into 16 clusters by pulsed-field gel electrophoresis and subjected to routine susceptibility testing to 12 antibiotics. Those isolates exhibited high resistance to penicillin (100%), erythromycin, trimethoprim-sulphamethoxazole (34.78%), oxacillin, clindamycin, and cefoxitin (21.74%). Multidrug-resistant (MDR)
S. aureus
was found in 34.78% (8 of 23) of isolates. Further,
S. aureus
strain DC.RB_015 isolated from
Rubing
cheese, recognized as the most resistant to six antibiotics, was selected for whole-genome sequencing (WGS), continued with in silico approaches.
S. aureus
DC.RB_015 had a single chromosome size of 2,794,578 bp and a plasmid size of 22,961 bp. The strain harbored 18 predicted ARGs, including eight efflux pump genes (
mepA
,
tet(K)
,
arlR
,
arlS
,
norA
,
mgrA
,
tet(38)
,
LmrS
), one peptidoglycan biosynthesis gene (
bacA
), two β-lactams resistance genes (
mecA, blaZ
), and seven genes conferring other antimicrobial resistance (
APH(3′)-IIIa
,
aad(6)
,
ErmB
,
SAT-4
,
mecR1
,
GlpT
,
murA
). The results of this study expand the knowledge of
S. aureus
strain DC.RB_015, increase food safety awareness, and will be helpful in establishing therapeutic therapy.
With the advantages of intrinsic safety, low price, high output potential, and acceptable energy density, aqueous rechargeable Zn–MnO2 batteries are gradually emerging as promising energy storage ...devices in recent years. Unfortunately, the structural instability and poor electrical conductivity of manganese dioxides still hinder their further applications. To tackle these issues, we demonstrate a high-performance cathode material for Zn–MnO2 batteries by hybridizing lignin-derived porous carbon with δ-MnO2 (denoted as LPC/δ-MnO2). Benefiting from the high electrical conductivity of porous carbon, the LPC/δ-MnO2 cathode material can offer high discharge capacity of 332.3 mAh g–1 at 0.2 A g–1 and excellent high-rate capability (196.1 mAh g–1 at 5 A g–1). Meanwhile, the assembled Zn–MnO2 battery displays good long-term cycling stability (82% capacity retention after 1000 cycles). Such superior performances are attributed to the synergetic effect of nanostructured δ-MnO2 and porous carbon scaffold, which is in favor of rapid Zn2+ ion diffusion and large contribution ratio of pseudocapacitive Zn2+ ion intercalation. The results of this study show great prospects of hybridizing biomass-derived carbon framework with electrochemically active materials toward advanced energy storage materials.
BCp12 is a novel casein-derived antibacterial peptide with a broad-spectrum antibacterial effect. However, its action mechanism against
is unknown. In this study, the growth curve showed that BCp12 ...had excellent antibacterial activity against
. Red (propidium iodide staining) and green (fluorescein isothiocyanate staining) fluorescence signals were detected at the edges of the
cells treated with BCp12. scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images showed that
cells became rough and shrunken, and part of the cell contents leaked to form a cavity. Furthermore, the iTRAQ proteome analysis showed that 193 and 174 proteins were significantly up-regulated and down-regulated, respectively, after BCp12 treatment. Four enzymes involved in fatty acid degradation of
were down-regulated, disrupting the synthesis of cell membranes. Molecular docking and gel retardation assays showed that BCp12 could bind to genes encoding four key enzymes involved in the fatty acid degradation pathway through hydrogen bonding and hydrophobic interactions, thus significantly inhibiting their activities. Overall, the results indicate that BCp12 inhibits the growth of
, causing metabolic disorders, thus destroying the structure of cell membranes.
This study aims to isolate the antimicrobial peptide (AMP) from buffalo casein hydrolyzed by Dregea sinensis Hemsl. protease. The AMP was isolated from hydrolysate by live bacteria adsorption, then ...analyzed using reversed-phase high-performance liquid chromatography, and the fraction with highest antimicrobial activity was identified by liquid chromatography-tandem MS. Further, we characterized the peptide in terms of its peptide sequence, structure, and antimicrobial activity. The results identified the AA sequence of the peptide as YLGYLEQLLRLK, which corresponds to residues 106 to 117 of bovine αS1-casein, and we named it BCp12. BCp12 displays α-helical structure, with high hydrophobic moments and net positive charge. BCp12 can inhibit the growth of indicator bacteria, with minimum inhibitory concentration values ranging from 0.8 to 1.6 mg/mL, and can induce low toxicity in mammalian cells. Antimicrobial activity of the BCp12 peptide remained stable under different salt concentrations but was sensitive to trypsin and high temperatures (121°C and above). The results support further research in the application of our newly generated AMP as an antimicrobial agent in the food industry and in food processing facilities.
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•The Zn foil is coated by carbon black to enlarge the electroactive surface area.•Nanofibrillated cellulose is used as an effective binder to adhere carbon black.•The modified anode ...can eliminate the dendritic growth and side reactions.•Excellent interface stability between the anode and electrolyte is achieved.•The Zn-MnO2 battery with modified anode shows significantly improved cyclability.
Aqueous zinc-ion batteries have received significant attention due to their low cost and high safety. However, the unsatisfactory cycling performances caused by the dendritic growth on the Zn anode limit their practical applications. Herein, we propose to modify the conventional Zn foil anode by using carbon black coating and nanofibrillated cellulose binder. The carbon black can form an electrically conductive network, thus greatly enlarging the electroactive surface area, while the nanofibrillated cellulose can act as an electrolyte reservoir to facilitate charge transports. Thanks to that, the modified anode can significantly eliminate the dendritic growth and side reactions, therefore ensuring excellent interface stability with the electrolyte even at a commercial-level areal capacity of 5 mAh g−1. With the modified anode, the Zn-MnO2 battery gives a high capacity retention of 87.4% after 1000 cycles, much higher than that with the unmodified Zn foil (42.6%). This study discloses a facile, scalable, and cost-effective strategy to achieve dendrite-free metal electrodes towards great cyclability.
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•Peptides with known bioactivities were observed in digested Chinese traditional cheeses, Rushan and Naizha.•Peptides from Rushan cheese digestion were distinct to Naizha ...cheese.•Minor milk proteins and κ-casein were the major source of peptides from Naizha cheese.•β-casein was the largest source of peptides from Rushan cheese.
Chinese Rushan and Naizha, the traditional acid coagulated cheese types produced from cow and yak milk, respectively, have been consumed for more than thousands of years. In this study, we aimed to characterise peptides of Rushan and Naizha in simulated in vitro gastrointestinal digestion using label-free based peptidomic. The identified peptide sequences were subjected to BIOPEP database driven bioactivity search. In total, 309 and 225 peptides were identified from Rushan and Naizha cheese, respectively, corresponding to 20 protein annotations. Analysis of label-free quantification found different protein digestibility, where casein was the primary source of peptides in Rushan, among which 62% represented β-casein by peptide count. The release of peptides was concentrated in specific residues 145–155 of β-casein in Rushan. In contrast, κ-casein and 7 minor milk proteins were dominant in digestion of Naizha cheese (p < 0.05). In particular, there were 11 peptides from digestion that were exact matches in databases to sequences with immunomodulatory, antibacterial, ACE-inhibition, DPP IV inhibition and antioxidant activities. Four novel angiotensin I-converting enzyme inhibitory (ACEI) activities peptides (YPFPGPIH, LKNWGEGW, RELEEIR, and HPHPHLS) were explored using molecular docking, chemically synthesized, and in vitro ACEI activity. The peptides had lower estimated free energy values (−5.34 to −7.66 kcal/mol), and exhibited the lowest IC50 value of 109.5, 77.7, 196.6, and 64.30 μM, respectively. Our study is the most comprehensive peptidomic analysis of Chinese Rushan and Naizha cheese to date.
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•Peptidomic provides insights into finding active peptides from the 1483 peptides identified.•Two potential anti-inflammatory peptides, DQPFFHYN and YSPFSSFPR, were excavated.•Two ...peptides with β-turned conformations exhibited potent thermal stability and safety in-vitro.•Two peptides suppressed the expression of iNOS, TNF-α, and IL-6 in inflammatory macrophages.•The discovery of peptides in milk by new technology can drive the development of dairy industry.
Whey protein hydrolysate from Binglangjiang buffalo, a unique genetic resource, has anti-inflammatory activity, but its anti-inflammatory composition and effects are unknown. The aim of this study was to investigate the anti-inflammatory peptides from Binglangjiang buffalo whey protein hydrolysate. A total of 1483 peptides were identified using LC-MS/MS, and 12 peptides were chosen for chemical synthesis using peptidomics, and then two novel anti-inflammatory peptides (DQPFFHYN (DN8) and YSPFSSFPR (YR9)) were screened out using LPS-stimulated RAW264.7 cells. The molecular weights of DN8 and YR9 with β-turn conformations were 1067.458 Da and 1087.52 Da, respectively, and showed a high in-vitro safety profile and thermal stability, but were intolerant to pepsin. Furthermore, ELISA and Western blot analysis indicated that peptides DN8 and YR9 significantly suppressed the secretion of pro-inflammatory cytokines NO, TNF-α, and IL-6 and the expression of mediators iNOS, TNF-α, and IL-6 in LPS-stimulated RAW264.7 cells. The study provides insights into the development of novel food-based anti-inflammatory nutritional supplements.
Moringa oleifer Lam. flower contain high-proteins and function nutrients. Many advances have been made to it, but there is still no proteomic information of this species. Total protein from the ...flowers applied shotgun 2DLC–MS/MS proteomic identified 9443 peptides corresponding to 4004 high-confidence proteins by Proteome Discoverer™ Software 2.1. These proteins were mostly distributed ranging between 40 and 70 kDa. Gene Ontology (GO) analysis indicated that the largest of the proteins were cytoplasm 72.7%, catalytic activity 61.5% and macromolecule metabolism 43.7%, and KEGG analysis revealed that the largest group of 129 proteins was involved in Ribosome to directing protein synthesis (translation). Moreover, a number of commercially important food-grade enzymes were commented, 261 proteins were annotated as carbohydrate-active enzymes, 16 protease, 22 proteins are assigned to the citrate cycle, which the top proteins were assigned to GH family, cysteine synthase and serine/threonine-protein phosphatase. These enzymes indicated that is a new source with potential use for fermentation and brewing industry, fruit and vegetable storage and the development of function peptides.
•The article presents the first detailed proteomic analysis of the flower of Moringa oleifer Lam. which contain high-proteins.•GH family, cysteine synthase and serine/threonine-protein phosphatase constituted the most abundant groups.•The discovery indicated several catalytic activity proteins, especially saccharide-degrading and milk-clotting enzymes.•The proteases and CAZyme analysis suggested their potential use in the food industry through further purification.
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