Abstract It is proposed that human decidua contains a population of stem cells that are responsible for the proliferation ability during the process of embryo implantation and placenta formation and ...that factors in the crosstalk between the decidua and chorion may mediate decidual stem cell differentiation. This study analysed the phenotype of side population (SP) cells and investigated the clonogenicity and differentiation ability of SP cells in human decidua of early pregnancy. Serum-free culture-conditioned media of human decidua and chorion were obtained from decidua and chorion explant culture. Decidual SP cells were isolated by fluorescence-activated cell sorting. Different inducing media were added and the functional differentiation of decidual SP cells was examined. Decidual SP cells were negative for the mature decidual cell marker CD13 and prolactin and negative for CD34 and CD45 expression. Decidual SP cells formed clones after culture in colony-forming medium and they could form clones again. Differentiated cells expressing CD13 and prolactin were observed and stroma-like structures expressing CD13 were obtained. These results indicate that decidual SP cells are enriched for stem cell activity. Oestradiol, progesterone and factors in culture-conditioned media of human decidua and chorion induced their proliferation and differentiation. The key point for successful pregnancy is when the embryo been accepted by the uterus of the mother, for which decidua formation and embryo development are involved. The decidua is the uterine lining (endometrium) during a pregnancy and the chorion is one of the membranes that exists between the developing fetus and mother. Poor development of the decidua can cause disease such as infertility. Stem cells are characterized by the ability to self-renew and the ability of daughter cells to differentiate into more specialist mature cells. An adult stem cell subpopulation (side population cells, SP cells) has been identified. It has been confirmed that the human endometrium contains stem cells that are responsible for its remarkable regeneration ability. Our previous study has already demonstrated that SP cells are present in decidua. The decidua and the chorion secrete factors important for maintenance of pregnancy. However, whether these factors can induce the differentiation of decidual SP cells remains unknown. In this study, we isolated and analysed the cell markers of SP cells in the human decidua of early pregnancy. To imitate the environment in vivo , we collected media after culture of the decidua or the chorion. After treatment with the media, decidual SP cells were studied for cell differentiation. Functional differentiation of decidual SP cells was observed and stroma-like structures were obtained. These results indicate that decidual SP cells are enriched for stem cell activity. Factors in culture-conditioned media from human decidua and chorion can induce SP cell proliferation and differentiation.
Goafs formed in mine-out areas threat the underground mines owing to possibilities of rock burst and surface subsidence. This paper aims to discuss the feasibility, design and construction of waste ...rock backfill in abandoned stopes. Based on goafs distribution and stabilities in the White Bull Mine, rock backfill system with a total volume of 362,000 m3 in 8 gobs was designed and carried out. The system included technologically and economically feasible stopes, conveying equipments of tramcars, rock transfer by electric rakes, haulage network and mining workings. Field effects showed that rock backfill was applicable to control potential hazards. With rock filling, goaf utilization rates ranged from 60% to 70%, which helped to control adjacent rock movement, eliminate rock burst and surface subsidence. Additionally, backfill construction reduced the lift and transportation costs of rocks by about 50%, which further lowered the land usage of surface dumping.
To study the antiinflammatory effects of naphtha from different chemotypes of Cinnamomum camphora and natural borneol on the rat arthritis model induced by Freund's adjuvant.
The arthritis model was ...induced by injecting Freund's adjuvant in rat voix pedis dermis and the rats were randomly divided into seven groups: normal control group, model control group, triptergium wilfordii control group, borneol chemotype naphtha group, camphor chemotype naphtha group, isocamphane chemotype naphtha group and natural borneol group. Rats of the triptergium wilfordii control group were given orally 8.1 mg x kg(-1) triptergium wilfordii for 35 days, rats of the normal control group and model control group were given same volume water, and rats of other groups were given 80 mg x kg(-1) corresponding drug. We observed the rat common condition, weighed the rat body weight weekly, measured the degree of swelling of voix pedis every 4 days, weighed the thymus and spleen on the end of life, and measured the contents of cell facto
Background and objective Traditional techniques in clinical diagnostic pathology have some limitations. Here we tried to develope frozen tissue microarray as a fast, simple and economical technique. ...Methods A total of 48 dots frozen tissue microarray were constructed in this study including primary 19 lung cancer samples and 5 normal lung tissue samples from primary lung cancer. Hematoxylin-Eosin and immunohistochemical stains were performed on the sections and we assess the feasibility on morphology and genetic expression. Results The cores on frozen tissue microarray of OCT block were regularly arranged and had no shift and distort. After HE-stained, morphology of the cores was well preserved. They were answered for the request of research.Immunohistochemistry of array slides: we performed immunohistochemistry on the tumor tissue microarray with antibodies for EMA. EMA staining is uniform across the sample and gives the expected cytochylema-associated staining. There is no background staining. We compared t
BackgroundXp11.2 translocation/TFE3 gene fusion associated with renal cell carcinoma (Xp11.2 RCC) exhibits unique biological characteristics and is associated with an increased incidence of tumor ...thrombosis, lymph node metastasis, and advanced disease stages. Multimodality imaging, including US, contrast-enhanced CT, multi-parametric MRI, and 18F-FDG PET/CT plays a crucial role in the preoperative diagnosis and differentiation of renal tumors. Case reportA 15-year-old female presented with lumbar pain worsened, and developed persistent painless hematuria. The CT attenuation values of the scan without contrast, corticomedullary phase, nephrographic phase, and delayed phases were 35 HU, 83 HU, 82 HU, and 75 HU, respectively. The solid component of the mass displayed heterogeneous marked enhancement. Furthermore, MRU indicated that the lesion involved the cortical medulla and infringed on the renal sinus fat. The lesion appeared isosignal in T1WI, slightly low signal in T2WI, and slightly high signal in DWI. The degree of enhancement in the three phases of enhancement scan was lower than that in the renal parenchyma, and hemorrhage and necrosis were observed within the internal part of the lesion. To further clarify the staging, the patient underwent 18F-FDG PET/CT. PET/CT images showed multiple irregular occupancies in the right kidney with unclear borders, showing a heterogeneous increase in 18F-FDG uptake, with SUVmax values ranging from 2.3 to 5.2 in the routine imaging phase (60 min post-injection), compared to SUVmax values ranging from 2.8 to 6.9 in the delayed imaging phase (160 min post-injection). Additionally, multiple enlarged and fused lymph nodes were observed in the medial part of the right kidney and the retroperitoneum, exhibiting a heterogeneous increase in 18F-FDG uptake, with SUVmax values ranging from 4.1 to 8.7 in the routine imaging phase, compared to SUVmax values ranging from 4.4 to 9.1 in the delayed imaging phase. The postoperative pathology, immunohistochemistry, and molecular analysis of histiocytes were consistent with a diagnosis of Xp11.2 RCC. One month after surgery, enhanced-CT examination of the patient revealed lung metastasis, peritoneal metastasis, and multiple lymph node metastases throughout the body, with an overall survival of 16 months. ConclusionXp11.2 RCC exhibits unique biological characteristics and is associated with an increased incidence of tumor thrombosis, lymph node metastasis, and advanced disease stages. Long-term follow-up is essential to monitor the likelihood of recurrence and metastasis. 18F-FDG PET/CT examination can comprehensively visualize the lesion's location and extent, providing a basis for clinical tumor staging and aiding in treatment monitoring and follow-up. To address the limitations of FDG, the utilization of specific tracers designed for RCC or tracers that are not excreted via the urinary system would be ideal. Further advancements in molecular imaging technologies and the development of novel tracers hold great promise in advancing the diagnosis and management of RCC, ultimately contributing to better patient outcomes and overall disease management.
