Helicosporous hyphomycetes are a morphologically allied group of Tubeufiales. We introduce a new helicosporous genus,
Neohelicosporium
, with five new species,
Neohelicosporium aquaticum
,
N. ...guangxiense
,
N. hyalosporum
,
N. parvisporum
, and
N. thailandicum
, based on morphological and phylogenetic evidence. The
RPB2
protein gene data are provided to analyze their phylogeny in Tubeufiales. Phylogenetic analyses of combined ITS, LSU,
RPB2
, and
TEF1α
sequence data from 13 new isolates of
Neohelicosporium
provided evidence to support the establishment of the new taxa. The morphological characters of
Neohelicosporium
that differentiate it from other helicosporous species are compared and discussed.
Tubeufia
was named for a sexual morph taxon. However, several asexual morph species have been accommodated in this genus as well. In our study, four new species of
Tubeufia
, viz.
T. filiformis
,
T. ...latispora
,
T. laxispora
and
T. mackenziei
, are described and illustrated. The phylogenetic placement of the new species is confirmed by analysis of combined ITS, LSU and
TEF1α
sequence data. A key to
Tubeufia
species is provided and the new species are compared with similar fungal taxa.
Cordycepin is one of the most important bioactive compounds produced by species of Cordyceps sensu lato, but it is hard to produce large amounts of this substance in industrial production. In this ...work, single factor design, Plackett-Burman design, and central composite design were employed to establish the key factors and identify optimal culture conditions which improved cordycepin production. Using these culture conditions, a maximum production of cordycepin was 2008.48 mg/L for 700 mL working volume in the 1000 mL glass jars and total content of cordycepin reached 1405.94 mg/bottle. This method provides an effective way for increasing the cordycepin production at a large scale. The strategies used in this study could have a wide application in other fermentation processes.
Tetraplosphaeriaceae (Pleosporales, Ascomycota) is a family with many saprobes recorded from various hosts, especially bamboo and grasses. During a taxonomic investigation of microfungi in tropical ...and subtropical forest regions of Guizhou, Hainan and Yunnan provinces, China, several plant samples were collected and examined for fungi. Four newly discovered species are described based on morphology and evolutionary relationships with their allies inferred from phylogenetic analyses derived from a combined dataset of LSU, ITS, SSU, and
DNA sequence data. Detailed illustrations, descriptions and taxonomic notes are provided for each species. The four new species of Tetraplosphaeriaceae reported herein are
,
,
, and
. A checklist of Tetraplosphaeriaceae species with available details on their ecology is also provided.
Aberrant self-renewal of leukemia initiation cells (LICs) drives aggressive acute myeloid leukemia (AML). Here, we report that UHRF1, an epigenetic regulator that recruits DNMT1 to methylate DNA, is ...highly expressed in AML and predicts poor prognosis. UHRF1 is required for myeloid leukemogenesis by maintaining self-renewal of LICs. Mechanistically, UHRF1 directly interacts with Sin3A-associated protein 30 (SAP30) through two critical amino acids, G572 and F573 in its SRA domain, to repress gene expression. Depletion of UHRF1 or SAP30 derepresses an important target gene, MXD4, which encodes a MYC antagonist, and leads to suppression of leukemogenesis. Further knockdown of MXD4 can rescue the leukemogenesis by activating the MYC pathway. Lastly, we identified a UHRF1 inhibitor, UF146, and demonstrated its significant therapeutic efficacy in the myeloid leukemia PDX model. Taken together, our study reveals the mechanisms for altered epigenetic programs in AML and provides a promising targeted therapeutic strategy against AML.
Seven undescribed guaiane-type sesquiterpenoids named biscogniauxiaols A-G (
-
) were isolated from the endophytic fungus
on
Their structures were determined by extensive spectroscopic analyses, ...electronic circular dichroism (EC) and specific rotation (SR) calculations. Compound
represented a new family of guaiane-type sesquiterpenoids featuring an unprecedented 5/6/6/7 tetracyclic system. A plausible biosynthetic pathway for compounds
-
was proposed. The anti-fungal, anti-inflammatory and multidrug resistance reversal activities of the isolates were evaluated. Compounds
,
and
exhibited potent inhibitory activities against
with MIC values ranging from 1.60 to 6.30 μM, and suppressed nitric oxide (NO) production with IC
ranging from 4.60 to 20.00 μM. Additionally, all compounds (100 μg/mL) enhanced the cytotoxicity of cisplatin in cisplatin-resistant non-small cell lung cancer cells (A549/DDP). This study opened up a new source for obtaining bioactive guaiane-type sesquiterpenoids and compounds
,
, and
were promising for further optimization as multifunctional inhibitors for anti-fungal (
.
) and anti-inflammatory purposes.
Eight hyphomycetes were collected as part of an investigation into the diversity of hyphomycetous fungi in China. Based on morphology and multi-loci (LSU, ITS, tef1α, and rpb2) phylogenetic analyses, ...five new taxa, including a new Aquapteridospora species A. hyalina and four novel Distoseptispora species, viz D. aquisubtropica, D. septata, D. tropica, and D. wuzhishanensis were introduced in Distoseptisporales (Sordariomycetes). Two new habitat records, viz Distoseptispora pachyconidia and D. xishuangbannaensis were firstly reported. Also provided in this study are detailed descriptions of eight new collections and a revised phylogenetic tree for the Distoseptisporales.
•This is the first report of endophytic Biscogniauxia in Dendrobium species•Biscogniauxia dendrobii sp. nov. is introduced based on the large discrepancy on both sequences (5.2%) and size of ...conidia.•The ethyl acetate crude extract of the isolate B. petrensis showed moderate and strong cytotoxic activity against A549 and K562 using CCK8 assay (IC50 of it 13.5 and 3.5 ug/ml, respectively).
Biscogniauxia belongs to the family Graphostromataceae, and species have been found mainly as pathogens in dicotyledonous plants worldwide. Two endophytic strains, Biscogniauxia dendrobii sp. nov. and B. petrensis were isolated from Dendrobium aphyllum and D. harveyanum sampled from southwestern China and northern Thailand respectively. The conidiophores and conidia were used for morphological examination. The maximum likelihood and Bayesian analysis with ITS, TUB2, RPB2 and LSU sequence data were combined for phylogenetic analyses and species identification. Biscogniauxia dendrobii sp. nov. is introduced based on the large DNA base pair differences (5.2%) and size of conidia. Another known species B. petrensis is also illustrated and described in this study to facilitate species identification on D. harveyanum. The ethyl acetate crude extracts of the isolate B. petrensis showed moderate and strong cytotoxic activity against A549 and K562 using the CCK8 assay (IC50 of it 13.5 and 3.5 ug/ml respectively). This is the first report of endophytic Biscogniauxia in Dendrobium species and of their cytotoxicity to A549 and K562, all of which enhances the current knowledge on the genus Biscogniauxia.
This article is the 15th contribution in the Fungal Diversity Notes series, wherein 115 taxa from three phyla, nine classes, 28 orders, 48 families, and 64 genera are treated. Fungal taxa described ...and illustrated in the present study include a new family, five new genera, 61 new species, five new combinations, one synonym, one new variety and 31 records on new hosts or new geographical distributions. Ageratinicolaceae
fam. nov.
is introduced and accommodated in Pleosporales. The new genera introduced in this study are
Ageratinicola
,
Kevinia
,
Pseudomultiseptospora
(Parabambusicolaceae),
Marasmiellomycena
, and
Vizzinia
(Porotheleaceae). Newly described species are
Abrothallus altoandinus
,
Ageratinicola kunmingensis
,
Allocryptovalsa aceris
,
Allophoma yuccae
,
Apiospora cannae
,
A. elliptica
,
A. pallidesporae
,
Boeremia wisteriae
,
Calycina papaeana
,
Clypeococcum lichenostigmoides
,
Coniochaeta riskali-shoyakubovii
,
Cryphonectria kunmingensis
,
Diaporthe angustiapiculata
,
D. campylandrae
,
D. longipapillata
,
Diatrypella guangdongense
,
Dothiorella franceschinii
,
Endocalyx phoenicis
,
Epicoccum terminosporum
,
Fulvifomes karaiensis
,
F. pannaensis
,
Ganoderma ghatensis
,
Hysterobrevium baoshanense
,
Inocybe avellaneorosea
,
I. lucida
,
Jahnula oblonga
,
Kevinia lignicola
,
Kirschsteiniothelia guangdongensis
,
Laboulbenia caprina
,
L. clavulata
,
L. cobiae
,
L. cosmodisci
,
L. nilotica
,
L. omalii
,
L. robusta
,
L. similis
,
L. stigmatophora
,
Laccaria rubriporus
,
Lasiodiplodia morindae
,
Lyophyllum agnijum
,
Marasmiellomycena pseudoomphaliiformis
,
Melomastia beihaiensis
,
Nemania guangdongensis
,
Nigrograna thailandica
,
Nigrospora ficuum
,
Oxydothis chinensis
,
O. yunnanensis
,
Petriella thailandica
,
Phaeoacremonium chinensis
,
Phialocephala chinensis
,
Phytophthora debattistii
,
Polyplosphaeria nigrospora
,
Pronectria loweniae
,
Seriascoma acutispora
,
Setoseptoria bambusae
,
Stictis anomianthi
,
Tarzetta tibetensis
,
Tarzetta urceolata
,
Tetraploa obpyriformis
,
Trichoglossum beninense
, and
Tricoderma pyrrosiae
. We provide an emendation for
Urnula ailaoshanensis Agaricus duplocingulatoides
var.
brevisporus
introduced as a new variety based on morphology and phylogeny.
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