Abstract Restless legs syndrome (RLS) is characterized by the association of an urge to move, and vesperal or nocturnal sensory symptoms; it is frequently associated with periodic limb movements. ...Evidence from imaging and electrophysiological studies suggests that RLS is linked to changes in sensorimotor integration. Nevertheless, the underlying mechanisms have not been characterized, and the cortical origin has yet to be confirmed. The objective of the present study was to establish whether or not sensorimotor integration in RLS patients is impaired in the evening. The time-dependent modulation of motor cortex excitability following peripheral electric nerve stimulation was studied in 14 idiopathic RLS patients, and 14 paired healthy controls. Different inter-stimulus intervals were used to measure short-latency and long-latency afferent inhibition (SAI and LAI) and afferent-induced facilitation (AIF). Motor evoked potentials were recorded from the first dorsal interosseous muscle in two experimental sessions (one in the morning and one in the evening). With the exception of LAI (which was present in the morning but absent in the evening in both healthy controls and RLS patients), no circadian variations were observed in sensorimotor integration. Although SAI was present in patients with RLS, AIF was disrupted (relative to controls) – suggesting the presence of an indirect sensorimotor integration disorder affecting the long corticocortical pathways in patients with RLS. The lack of circadian modulation in sensorimotor integration suggests that clinical circadian variations have other causes.
Multiple sclerosis (MS) is the most common chronic inflammatory neurological disease. The emergence of disease-modifying therapies (DMTs) has greatly improved disease activity control and progression ...of disability in MS patients. DMTs differ in their mode of action, route of administration, efficacy, and safety profiles, offering multiple options for clinicians. Personalized medicine aims at tailoring the therapeutic strategy to patients’ characteristics and disease activity but also patients’ needs and preferences. New therapeutic options have already changed treatment paradigms for patients with active relapsing MS (RMS). The traditional approach consists in initiating treatment with moderate-efficacy DMTs and subsequently, escalating to higher-efficacy DMTs when there is evidence of clinical and/or radiological breakthrough activity. Recent real-world studies suggest that initiation of high-efficacy DMTs from disease onset can improve long-term outcomes for RMS patients. In this article, we review different treatment strategies and discuss challenges associated with personalized therapy.
We performed an online survey to assess lockdown impact in 176 patients with multiple sclerosis (PwMS) in the north of France. Access to healthcare was reduced for 38% of PwMS, mainly in ...physiotherapy, general practitioners and neurologists. 49.2% have implemented self-rehabilitation programs. Medical support was maintained for 39.2% through teleconsultations. 76.2% reported a negative impact of lockdown related to worsen disability. 45.5% expressed beneficial effects like strengthening family relationships, and reduced fatigue. Previous studies have found the same results on disability and discontinuation of care. However, even if this period has been challenging for PwMS, most of them have shown excellent adaptability.
The paper presents the effect of conventional (use of NPK mineral fertilizers and pesticides) and organic (no use of agrochemicals) farming systems on selected parameters of antioxidant properties of ...winter wheat, spring barley and oat grain. The research was carried out during the period 2017–2019 at the Czesławice Experimental Farm (central Lublin region, Poland) on loess soil (second quality class). The aim of the research was to evaluate the functional (antioxidant) properties of winter wheat, spring barley and oat grain in whole grain and its milling fractions (dehulled grain, flour and bran). The reduction potential (Fe+3 → Fe+2 ), the ability to eliminate the free DPPH• radical and the total antioxidant potential in the β-carotene/linoleic acid system were determined. Polyphenol content was also determined using Folin–Ciocalteau reagent. The organic system did not significantly increase the antioxidant properties of cereal grains compared to the conventional system. Under organic farming conditions, oat grain was characterised only by the most favourable antioxidant properties. A highly statistically significant correlation was found between total polyphenol content and DPPH• free radical quenching capacity, especially for oat and barley in the organic system. The closest correlations were for the fractions of bran and whole grain. Dehulling of grain, with the exception of oat grain, irrespective of the farming system, resulted in a significant deterioration of the antioxidant potential of grain extracts. In summary, the study showed that the bran obtained from oat grown under an organic system had the strongest antioxidant activity.
If the mass excess of neutron-deficient nuclei and their neutron-rich mirror partners are both known, it can be shown that deviations of the isobaric mass multiplet equation (IMME) in the form of a ...cubic term can be probed. Such a cubic term was probed by using the atomic mass of neutron-rich magnesium isotopes measured using the TITAN Penning trap and the recently measured proton-separation energies of 29Cl and 30Ar. The atomic mass of 27Mg was found to be within 1.6σ of the value stated in the Atomic Mass Evaluation. The atomic masses of 28,29Mg were measured to be both within 1σ, while being 7 and 33 times more precise, respectively. Using the 29Mg mass excess and previous measurements of 29Cl, we uncovered a cubic coefficient of d = 28(7)keV, which is the largest known cubic coefficient of the IMME. This departure, however, could also be caused by experimental data with unknown systematic errors. Hence there is a need to confirm the mass excess of 28S and the one-neutron separation energy of 29Cl, which have both come from a single measurement. Lastly, our results were compared with ab initio calculations from the valence-space in-medium similarity renormalization group, resulting in a good agreement.
Abstract
Penning trap mass spectrometry is the tool of choice for mass measurements to test the Standard Model or lay the nuclear-physics foundation of neutrino physics due to the high precisions ...achievable. This precision can be further boosted by higher charge states (Ettenauer et al 2011). For this purpose, an electron beam ion trap (EBIT) provides radioactive HCIs at the TITAN facility at TRIUMF. To improve the electron beam properties and its control, a new electron gun is under development. The electron gun within its TITAN EBIT environment was simulated using Field Precision’s TRAK software. A new electrode geometry was chosen and optimized to extract up to 5A, 66 keV electron beams. Due to the strong fringe field of the unshielded 6T magnet, options for the passive and active shielding of the gun were explored to compress the electron beam. During the design process, careful attention was paid to safety and mechanical considerations. Simulations and the status of the new electron gun will be presented.
Mutations in the DYSF gene, encoding the protein dysferlin, lead to several forms of muscular dystrophy. In healthy skeletal muscle, dysferlin concentrates in the transverse tubules and is involved ...in repairing the sarcolemma and stabilizing Ca2+ signaling after membrane disruption. The DYSF gene encodes 7–8 C2 domains, several Fer and Dysf domains, and a C-terminal transmembrane sequence. Because its coding sequence is too large to package in adeno-associated virus, the full-length sequence is not amenable to current gene delivery methods. Thus, we have examined smaller versions of dysferlin, termed “nanodysferlins,” designed to eliminate several C2 domains, specifically C2 domains D, E, and F; B, D, and E; and B, D, E, and F. We also generated a variant by replacing eight amino acids in C2G in the nanodysferlin missing domains D through F. We electroporated dysferlin-null A/J mouse myofibers with Venus fusion constructs of these variants, or as untagged nanodysferlins together with GFP, to mark transfected fibers We found that, although these nanodysferlins failed to concentrate in transverse tubules, three of them supported membrane repair after laser wounding while all four bound the membrane repair protein, TRIM72/MG53, similar to WT dysferlin. By contrast, they failed to suppress Ca2+ waves after myofibers were injured by mild hypoosmotic shock. Our results suggest that the internal C2 domains of dysferlin are required for normal t-tubule localization and Ca2+ signaling and that membrane repair does not require these C2 domains.
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Bloch and colleagues created small versions of dysferlin (“nanodysferlins”), potentially packageable in AAV, to learn if they restore the wild-type phenotype in dysferlin-null skeletal muscle. The four nanodysferlins assayed, containing three to four of dysferlin’s seven C2 domains, support membrane repair. None target triad junctions or support normal Ca2+ signaling, however.
Dislocations are one-dimensional defects in crystals, enabling their deformation, mechanical response, and transport properties. Less well known is their influence on material chemistry. The severe ...lattice distortion at these defects drives solute segregation to them, resulting in strong, localized spatial variations in chemistry that determine microstructure and material behavior. Recent advances in atomic-scale characterization methods have made it possible to quantitatively resolve defect types and segregation chemistry. As shown here for a Pt-Au model alloy, we observe a wide range of defect-specific solute (Au) decoration patterns of much greater variety and complexity than expected from the Cottrell cloud picture. The solute decoration of the dislocations can be up to half an order of magnitude higher than expected from classical theory, and the differences are determined by their structure, mutual alignment, and distortion field. This opens up pathways to use dislocations for the compositional and structural nanoscale design of advanced materials.
Chamomile is a herbal plant of very high economic importance worldwide. Its organically grown raw material is particularly valuable. Under organic farming conditions, weeds and fungal diseases are an ...important problem in a chamomile plantation. Seeking agronomic solutions designed to eliminate the occurrence of these pathogens in chamomile crops is constantly valid. The aim of the present study was to evaluate the effect of some foliar sprays (enhancing the condition of the crop plant and its competition against pathogens) and different row spacing of two chamomile cultivars on weed infestation and health of a chamomile plantation. The study results presented in this paper were collected from field experiments carried out in the organic system in the village of Dys (the central Lublin region, Poland) over the period 2014–2016. Experiments were conducted on podzolic soil (class III) as a split-block design in 3 replicates in plots with an area of 525 m2 (6.25 m2 a single plot). This study included two chamomile cultivars (“Złoty Łan”, “Mastar”). The second experimental factor was single or double foliar application of three bioproducts (Herbagreen Basic, Bio-algeen, Effective Microorganisms—EM Farming). The other experimental factor was a different row spacing of chamomile (40 cm and 30 cm). The obtained study results show that 10–16 annual weed species and 1–3 perennial species occurred in both chamomile cultivars. Foliar application of the bioproducts contributed to a reduction in the total number of weeds in the crop, but at the same time to greater weed species diversity. In the control treatments (without the bioproducts), the dominance of several weed species (Viola arvensis, Galeopsis tetrahit, Spergula arvensis, Juncus bufonius, Scleranthus annuus) and lower biodiversity of the weed flora were observed. The largest reduction (by about 20%) in the number of annual weeds was found under the influence of the bioproducts Herbagreen Basic and Bio-algeen applied once. Bio-algeen and Effective Microorganisms (EM), in turn, had a significant effect on decreasing the weed weight. A narrower (30 cm) row spacing of chamomile had a significant impact on reducing the weight of weeds in chamomile crops compared to the wider spacing, which was 40 cm. It should be concluded that infection of the chamomile plantation with fungal diseases was overall at a low level. Significantly higher infection with fungal diseases was found in the case of the cultivar “Mastar”, regardless of the experimental factors. A statistically proven decrease in infection of chamomile plants with fungal diseases was determined under lower crop density conditions (a row spacing of 40 cm). Chamomile plants were found to exhibit better health under the influence of double application of the biofertilizers Herbagreen Basic and Bio-algeen.
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