The microstructures and mechanical properties of cobalt-chromium (Co-Cr) alloys produced by three CAD/CAM-based processing techniques were investigated in comparison with those produced by the ...traditional casting technique. Four groups of disc- (microstructures) or dumbbell- (mechanical properties) specimens made of Co-Cr alloys were prepared using casting (CS), milling (ML), selective laser melting (SLM), and milling/post-sintering (ML/PS). For each technique, the corresponding commercial alloy material was used. The microstructures of the specimens were evaluated via X-ray diffractometry, optical and scanning electron microscopy with energy-dispersive X-ray spectroscopy, and electron backscattered diffraction pattern analysis. The mechanical properties were evaluated using a tensile test according to ISO 22674 (
= 6). The microstructure of the alloys was strongly influenced by the manufacturing processes. Overall, the SLM group showed superior mechanical properties, the ML/PS group being nearly comparable. The mechanical properties of the ML group were inferior to those of the CS group. The microstructures and mechanical properties of Co-Cr alloys were greatly dependent on the manufacturing technique as well as the chemical composition. The SLM and ML/PS techniques may be considered promising alternatives to the Co-Cr alloy casting process.
The influence of residual stress induced by sandblasting the zirconia ceramic surface on the resin bonding to the ceramic is still unclear. The effect of four different sandblasting conditions (with ...50 and 110 μm alumina at pressures of 0.2 and 0.4 MPa) on the bonding of adhesive resin cement (Panavia F 2.0) to zirconia (Cercon
ht) was investigated in terms of residual stress. The surface roughness and water contact angle of the zirconia surfaces were measured. The tetragonal-to-monoclinic (t-m) phase transformation and residual stresses (sin
method) were studied by X-ray diffraction. The resin-bonded zirconia specimens were subjected to shear bond strength (SBS) tests before and after thermocycling (10,000 and 30,000 cycles) (
= 10). As the particle size and pressure increased, the roughness gradually and significantly increased (
= 0.023). However, there were no significant differences in roughness-corrected contact angle among all the sandblasted groups (
> 0.05). As the particle size and pressure increased, the m-phase/(t-phase + m-phase) ratios and compressive residual stresses gradually increased. After thermocycling, there were no significant differences in SBS among the sandblasted zirconia groups (
> 0.05). In conclusion, increased surface roughness and residual stress do not directly affect the resin bonding durability.
Abstract Statement of problem Although new digital manufacturing techniques are attracting interest in dentistry, few studies have comprehensively investigated the marginal fit of fixed dental ...prostheses fabricated with such techniques. Purpose The purpose of this in vitro microcomputed tomography (μCT) study was to evaluate the marginal fit of cobalt-chromium (Co-Cr) alloy copings fabricated by casting and 3 different computer-aided design and computer-aided manufacturing (CAD-CAM)-based processing techniques and alloy systems. Material and methods Single Co-Cr metal crowns were fabricated using 4 different manufacturing techniques: casting (control), milling, selective laser melting, and milling/sintering. Two different commercial alloy systems were used for each fabrication technique (a total of 8 groups; n=10 for each group). The marginal discrepancy and absolute marginal discrepancy of the crowns were determined with μCT. For each specimen, the values were determined from 4 different regions (sagittal buccal, sagittal lingual, coronal mesial, and coronal distal) by using imaging software and recorded as the average of the 4 readings. For each parameter, the results were statistically compared with 2-way analysis of variance and appropriate post hoc analysis (using Tukey or Student t test) ( α =.05). Results The milling and selective laser melting groups showed significantly larger marginal discrepancies than the control groups (70.4 ±12.0 and 65.3 ±10.1 μm, respectively; P <.001), whereas the milling/sintering groups exhibited significantly smaller values than the controls ( P =.004). The milling groups showed significantly larger absolute marginal discrepancy than the control groups (137.4 ±29.0 and 139.2 ±18.9 μm, respectively; P <.05). In the selective laser melting and milling/sintering groups, the absolute marginal discrepancy values were material-specific ( P <.05). Nonetheless, the milling/sintering groups yielded statistically comparable ( P =.935) or smaller ( P <.001) absolute marginal discrepancies to the control groups. Conclusions The findings of this in vitro μCT study showed that the marginal fit values of the Co-Cr alloy greatly depended on the fabrication methods and, occasionally, the alloy systems. Fixed dental prostheses produced by using the milling/sintering technique can be considered clinically acceptable in terms of marginal fit.
To understand the mechanisms underlying tooth morphogenesis, we examined the developmental roles of important posttranslational modification, O‐GlcNAcylation, which regulates protein stability and ...activity by the addition and removal of a single sugar (O‐GlcNAc) to the serine or threonine residue of the intracellular proteins. Tissue and developmental stage‐specific immunostaining results against O‐GlcNAc and O‐GlcNAc transferase (OGT) in developing tooth germs would suggest that O‐GlcNAcylation is involved in tooth morphogenesis, particularly in the cap and secretory stage. To evaluate the developmental function of OGT‐mediated O‐GlcNAcylation, we employed an in vitro tooth germ culture method at E14.5, cap stage before secretory stage, for 1 and 2 days, with or without OSMI‐1, a small molecule OGT inhibitor. To examine the mineralization levels and morphological changes, we performed renal capsule transplantation for one and three weeks after 2 days of in vitro culture at E14.5 with OSMI‐1 treatment. After OGT inhibition, morphological and molecular alterations were examined using histology, immunohistochemistry, real‐time quantitative polymerase chain reaction, in situ hybridization, scanning electron microscopy, and ground sectioning. Overall, inhibition of OGT resulted in altered cellular physiology, including proliferation, apoptosis, and epithelial rearrangements, with significant changes in the expression patterns of β‐catenin, fibroblast growth factor 4 (fgf4), and sonic hedgehog (Shh). Moreover, renal capsule transplantation and immunolocalizations of Amelogenin and Nestin results revealed that OGT‐inhibited tooth germs at cap stage exhibited with structural changes in cuspal morphogenesis, amelogenesis, and dentinogenesis of the mineralized tooth. Overall, we suggest that OGT‐mediated O‐GlcNAcylation regulates cell signaling and physiology in primary enamel knot during tooth development, thus playing an important role in mouse molar morphogenesis.
Few studies have investigated changes in the marginal fit of metal-ceramic restorations fabricated by selective laser melting (SLM) techniques after the application of veneering ceramic.
The purpose ...of this in vitro study was to evaluate the marginal fit (silicone replica technique) and internal porosity (cross-section analysis) of cobalt-chromium (Co-Cr) alloy metal crowns prepared by using 2 SLM processes together with a casting technique before and after ceramic veneering.
Cast single Co-Cr crowns and SLM-processed crowns with large (SLML) or small (SLMS) porosity were prepared (n=20/group), and half were subjected to ceramic veneering. On a single Co-Cr master die, the marginal discrepancy (MD) and absolute marginal discrepancy (AMD) of the crowns were measured by using the silicone replica technique, in which each replica was cut into 4 sections before and after ceramic veneering (n=10 for each subgroup). After marginal fit measurements, each metal coping was cross-sectioned into 4 parts, and 5 rectangular optical microscope images were acquired on both outer corners of each quarter. The porosity was then calculated as the ratio of the black-to-white pixels on the binarized images. The data were analyzed by 2-way ANOVA and the post hoc test (Tukey or Student t test) (α=.05).
Before ceramic veneering, the 2 SLM groups showed significantly larger MDs than the casting group (56.4 ±10.4 μm) (P<.05). A significant increase in MD after ceramic veneering was detected only in the SLML group (P<.001). The AMD values showed a similar trend with MD values. The 2 SLM groups (in particular, SLML) showed a significantly higher amount of porosity than the casting group before ceramic veneering (P<.001). Only the SLML group showed a significant decrease in the amount of porosity after ceramic veneering (P<.001).
Within the limitations of this in vitro study, large internal porosity within the SLM-fabricated Co-Cr metal copings affected the marginal fit of the metal-ceramic crowns. However, all the MD values of the 3 groups were lower than the acceptable range even after the application of veneering ceramic.
Titanium (Ti) and Ti-based alloys are commonly used in dental implants, and surface modifications of dental implants are important for achieving osseointegration (i.e., direct connection between the ...implant surface and bone). This study investigated the effect of an eco-friendly etching solution—a hydrogen peroxide–sodium bicarbonate mixture—on the surface properties and contact angles of osteoblast adhesion and proliferation on Ti surfaces. Disk-shaped Ti specimens were prepared using different surface treatments (machining, sandblasting, and sandblasting/acid-etching), and they were immersed in the etching solution and ultrasonically cleaned. Surface characterization was performed using scanning electron microscopy, digital microscopy, contact angle analysis, and X-ray photoelectron spectroscopy. MG-63 osteoblasts were cultured on the specimens, and their adhesion to the specimen surface and proliferation were examined using staining and the MTT assay, respectively. Additional etching with the etching solution caused the formation of nano/micro hierarchical structures, increased surface roughness, and enhanced hydrophilicity. Osteoblast adhesion and proliferation were found to improve on the modified surfaces. The eco-friendly etching method has the potential to enhance the biological properties of Ti implant surfaces and thereby improve dental implant performance.
The purpose of this study was to evaluate whether the incorporation of an acidic monomer into the pulp-capping material TheraCal LC, which has a weak dentin bonding, increases the shear bond strength ...(SBS) to dentin. Di-2-hydroxyethyl methacryl hydrogenphosphate was incorporated into the material at 0.0 (TL0, control), 5.0 (TL5), and 10.0 (TL10) wt%. The water contact angle (CA) and mechanical properties for each material were also studied (
= 6). Debonding was performed at two different times (immediate and after 24 h) (
= 12). Hydroxyl and calcium ion releases in water at 37 °C were monitored up to 28 days (
= 6). The addition of the acidic monomer decreased the CAs (
< 0.001) and increased the flexural moduli (
< 0.001). The debonding time did not significantly affect the SBS values (
= 0.600). The TL10 group exhibited the highest SBS values, followed by the TL5 group. The TL10 group released significantly more calcium ions than the other two groups from 3 days (
< 0.05). The incorporation of the acidic monomer at 10.0 wt% into TheraCal LC enhanced bonding to dentin, while not negatively affecting the mechanical properties and ion-leaching capacity of the material.
Titanium nitride (TiN) was deposited on the surface of a cobalt-chromium (Co-Cr) alloy by a hot-wall type chemical vapor deposition (CVD) reactor at 850 °C, and the coating characteristics were ...compared with those of a physical vapor deposition (PVD) TiN coating deposited on the same alloy at 450 °C. Neither coating showed any reactions at the interface. The face-centered cubic (fcc) structure of the alloy was changed into a hexagonal close-packed (hcp) phase, and recrystallization occurred over at 10 μm of depth from the surface after CVD coating. Characteristic precipitates were also generated incrementally depending on the depth, unlike the precipitates in the matrix of the as-cast alloy. On the other hand, the microstructure and phase of the PVD-coated alloy did not change. Depth-dependent nano-hardness measurements showed a greater increase in hardness in the recrystallization zone of the CVD-coated alloy than in the bulk center of the alloy. The CVD coating showed superior adhesion to the PVD coating in the progressive scratch test. The as-cast, PVD-coated, and CVD-coated alloys all showed negative cytotoxicity. Within the limitations of this study, CVD TiN coating to biomedical Co-Cr alloy may be considered a promising alternative to PVD technique.
Abstract Objectives To measure the shear bond strengths of various luting cements to a sandblasted zirconia ceramic and to determine the surface energy parameters of the luting cements. Methods Two ...conventional glass ionomer cements, two resin-modified glass ionomer cements, two compomer cements, and two adhesive resin cements were prepared and bonded to sandblasted zirconia (Lava). All bonded specimens were stored in water at 37 °C for 48 h and then half of them additionally thermocycled 10,000 times prior to the shear bond strength test ( n = 10). Surface roughness ( Ra ) values and surface energy parameters of the eight luting cements and polished zirconia ceramic were evaluated using a profilometer and contact angle measurements, respectively ( n = 10). The bond strength and surface roughness data were statistically analysed using non-parametric and parametric procedures, respectively ( α = 0.05). Relationships between surface energy parameters and measured shear bond strengths were investigated using the Spearman rank correlation test. Results Panavia F 2.0 and Principle produced higher bond strengths than the other cements, with no significant changes before and after thermocycling. Fuji I, Ketac Cem Easymix, and Ionotite F yielded near-zero or zero values after thermocycling. All debonded specimens showed adhesive failure. Mean Ra values ranged from 0.104 to 0.167 μm. We found the base (hydrogen bond accepting) components of the luting cements significantly affected the bond strengths both before and after thermocycling. Conclusion It is recommended that the surface energy parameters of luting cements be considered in evaluating their adhesive properties with zirconia ceramic.
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