ABSTRACT
We present an analysis of a newly discovered 2+1 + 1 quadruple system with TESS containing an unresolved eclipsing binary (EB) as part of TIC 121088960 and a close neighbour TIC 121088959. ...The EB consists of two very low-mass M dwarfs in a highly eccentric (e = 0.709) short-period (P = 3.043 58 d) orbit. Given the large pixel size of TESS and the small separation (3${_{.}^{\prime\prime}}$9) between TIC 121088959 and TIC 121088960 we used light centroid analysis of the difference image between in-eclipse and out-of-eclipse data to show that the EB likely resides in TIC 121088960, but contributes only ∼10 per cent of its light. Radial velocity data were acquired with iSHELL at NASA’s Infrared Facility and the Coudé spectrograph at the McDonald 2.7-m telescope. For both images, the measured RVs showed no variation over the 11 d observational baseline, and the RV difference between the two images was 8 ± 0.3 km s−1. The similar distances and proper motions of the two images indicate that TIC 121088959 and TIC 121088960 are a gravitationally bound pair. Gaia’s large RUWE and astrometric_excess_noise parameters for TIC 121088960, further indicate that this image is the likely host of the unresolved EB and is itself a triple star. We carried out an SED analysis and calculated stellar masses for the four stars, all of which are in the M dwarf regime: 0.19 M⊙ and 0.14 M⊙ for the EB stars and 0.43 M⊙ and 0.39 M⊙ for the brighter visible stars, respectively. Lastly, numerical simulations show that the orbital period of the inner triple is likely the range 1–50 yr.
Bacterial survival is fraught with antagonism, including that deriving from viruses and competing bacterial cells. It is now appreciated that bacteria mount complex antiviral responses; however, ...whether a coordinated defense against bacterial threats is undertaken is not well understood. Previously, we showed that
possess a danger-sensing pathway that is a critical fitness determinant during competition against other bacteria. Here, we conducted genome-wide screens in
that reveal three conserved and widespread interbacterial antagonism resistance clusters (
). We find that although
are coordinately activated by the Gac/Rsm danger-sensing system, they function independently and provide idiosyncratic defense capabilities, distinguishing them from general stress response pathways. Our findings demonstrate that Arc3 family proteins provide specific protection against phospholipase toxins by preventing the accumulation of lysophospholipids in a manner distinct from previously characterized membrane repair systems. These findings liken the response of
to bacterial threats to that of eukaryotic innate immunity, wherein threat detection leads to the activation of specialized defense systems.
We present a quintuple star system that contains two eclipsing binaries. The unusual architecture includes two stellar images separated by 11 arcsec on the sky: EPIC 212651213 and EPIC 212651234. The ...more easterly image (212651213) actually hosts both eclipsing binaries which are resolved within that image at 0.09 arcsec, while the westerly image (212651234) appears to be single in adaptive optics (AO), speckle imaging, and radial velocity (RV) studies. The ‘A’ binary is circular with a 5.1-d period, while the ‘B’ binary is eccentric with a 13.1-d period. The γ velocities of the A and B binaries are different by ∼10 km s−1. That, coupled with their resolved projected separation of 0.09 arcsec, indicates that the orbital period and separation of the ‘C’ binary (consisting of A orbiting B) are ≃65 yr and ≃25 au, respectively, under the simplifying assumption of a circular orbit. Motion within the C orbit should be discernible via future RV, AO, and speckle imaging studies within a couple of years. The C system (i.e. 212651213) has an RV and proper motion that differ from that of 212651234 by only ∼1.4 km s−1 and ∼3 mas yr−1. This set of similar space velocities in three dimensions strongly implies that these two objects are also physically bound, making this at least a quintuple star system.
Context. Stars can maintain their observable magnetic activity from the pre-main sequence (PMS) to the tip of the red giant branch. However, the number of known active giants is much lower than ...active stars on the main sequence (MS) since the stars spend only about 10% of their MS lifetime on the giant branch. Due to their rapid evolution it is difficult to estimate the stellar parameters of giant stars. A possibility for obtaining more reliable stellar parameters for an active giant arises when it is a member of an eclipsing binary system. Aims. We have discovered EPIC 211759736, an active spotted giant star in an eclipsing binary system during the Kepler K2 Campaign 5. The eclipsing nature allows us to much better constrain the stellar parameters than in most cases of active giant stars. Methods. We have combined the K2 data with archival HATNet, ASAS, and DASCH photometry, new spectroscopic radial velocity measurements, and a set of follow-up ground-based BVRCIC photometric observations, to find the binary system parameters as well as robust spot models for the giant at two different epochs. Results. We determined the physical parameters of both stellar components and provide a description of the rotational and long-term activity of the primary component. The temperatures and luminosities of both components were examined in the context of the Hertzsprung–Russell diagram. We find that both the primary and the secondary components deviate from the evolutionary tracks corresponding to their masses in the sense that the stars appear in the diagram at lower masses than their true masses. Conclusions. We further evaluate the proposition that traditional methods generally result in higher masses for active giants than what is indicated by stellar evolution tracks in the HR diagram. A possible reason for this discrepancy could be a strong magnetic field, since we see greater differences in more active stars.
Disks in binary systems can cause exotic eclipsing events. MWC 882 (BD -22 4376, EPIC 225300403) is such a disk-eclipsing system identified from observations during Campaign 11 of the K2 mission. We ...propose that MWC 882 is a post-Algol system with a B7 donor star of mass in a 72-day orbit around an A0 accreting star of mass . The disk around the accreting star occults the donor star once every orbit, inducing 19-day long, 7% deep eclipses identified by K2 and subsequently found in pre-discovery All-Sky Automated Survey and All Sky Automated Survey for Supernovae observations. We coordinated a campaign of photometric and spectroscopic observations for MWC 882 to measure the dynamical masses of the components and to monitor the system during eclipse. We found the photometric eclipse to be gray to 1%. We found that the primary star exhibits spectroscopic signatures of active accretion, and we observed gas absorption features from the disk during eclipse. We suggest that MWC 882 initially consisted of a 3.6 M donor star transferring mass via Roche lobe overflow to a 2.1 M accretor in a 7-day initial orbit. Through angular momentum conservation, the donor star is pushed outward during mass transfer to its current orbit of 72 days. The observed state of the system corresponds with the donor star having left the red giant branch ∼0.3 Myr ago, terminating active mass transfer. The present disk is expected to be short-lived (102 yr) without an active feeding mechanism, presenting a challenge to this model.
Fusobacterium nucleatum (Fn) is a dominant bacterial species in colorectal cancer (CRC) tissue that is associated with cancer progression and poorer patient prognosis. Following a small-molecule ...inhibitor screen of 1,846 bioactive compounds against a Fn CRC isolate, we find that 15% of inhibitors are antineoplastic agents including fluoropyrimidines. Validation of these findings reveals that 5-fluorouracil (5-FU), a first-line CRC chemotherapeutic, is a potent inhibitor of Fn CRC isolates. We also identify members of the intratumoral microbiota, including Escherichia coli, that are resistant to 5-FU. Further, CRC E. coli isolates can modify 5-FU and relieve 5-FU toxicity toward otherwise-sensitive Fn and human CRC epithelial cells. Lastly, we demonstrate that ex vivo patient CRC tumor microbiota undergo community disruption after 5-FU exposure and have the potential to deplete 5-FU levels, reducing local drug efficacy. Together, these observations argue for further investigation into the role of the CRC intratumoral microbiota in patient response to chemotherapy.
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•5-Fluorouracil (5-FU) inhibits growth of the oncomicrobe Fusobacterium nucleatum•CRC strains of Escherichia coli rapidly modify 5-FU into a nontoxic product•Bacterially modified 5-FU no longer inhibits cancer or F. nucleatum cell growth•50% of patient-derived ex vivo CRC microbial communities can reduce 5-FU levels
LaCourse et al. show that Fusobacterium nucleatum, a bacterium associated with colorectal cancer, is inhibited by the chemotherapeutic 5-fluorouracil, suggesting that 5-fluorouracil efficacy may in part be due to its antimicrobial activity. Specific intratumoral microbiota members modify 5-fluorouracil into a nontoxic form, rescuing Fusobacterium nucleatum and cancer epithelial cell growth.
Single-cell RNA sequencing (scRNAseq) technologies have been beneficial in revealing and describing cellular heterogeneity within mammalian tissues, including solid tumors. However, many of these ...techniques apply poly(A) selection of RNA, and thus have primarily focused on determining the gene signatures of eukaryotic cellular components of the tumor microenvironment. Microbiome analysis has revealed the presence of microbial ecosystems, including bacteria and fungi, within human tumor tissues from major cancer types. Imaging data have revealed that intratumoral bacteria may be located within epithelial and immune cell types. However, as bacterial RNA typically lacks a poly(A) tail, standard scRNAseq approaches have limited ability to capture this microbial component of the tumor microenvironment. To overcome this, we describe the invasion-adhesion-directed expression sequencing (INVADEseq) approach, whereby we adapt 10x Genomics 5' scRNAseq protocol by introducing a primer that targets a conserved region of the bacterial 16S ribosomal RNA gene in addition to the standard primer for eukaryotic poly(A) RNA selection. This 'add-on' approach enables the generation of eukaryotic and bacterial DNA libraries at eukaryotic single-cell level resolution, utilizing the 10x barcode to identify single cells with intracellular bacteria. The INVADEseq method takes 30 h to complete, including tissue processing, sequencing and computational analysis. As an output, INVADEseq has shown to be a reliable tool in human cancer cell lines and patient tumor specimens by detecting the proportion of human cells that harbor bacteria and the identities of human cells and intracellular bacteria, along with identifying host transcriptional programs that are modulated on the basis of associated bacteria.
Fusobacterium nucleatum (Fn), a bacterium present in the human oral cavity and rarely found in the lower gastrointestinal tract of healthy individuals
, is enriched in human colorectal cancer (CRC) ...tumours
. High intratumoural Fn loads are associated with recurrence, metastases and poorer patient prognosis
. Here, to delineate Fn genetic factors facilitating tumour colonization, we generated closed genomes for 135 Fn strains; 80 oral strains from individuals without cancer and 55 unique cancer strains cultured from tumours from 51 patients with CRC. Pangenomic analyses identified 483 CRC-enriched genetic factors. Tumour-isolated strains predominantly belong to Fn subspecies animalis (Fna). However, genomic analyses reveal that Fna, considered a single subspecies, is instead composed of two distinct clades (Fna C1 and Fna C2). Of these, only Fna C2 dominates the CRC tumour niche. Inter-Fna analyses identified 195 Fna C2-associated genetic factors consistent with increased metabolic potential and colonization of the gastrointestinal tract. In support of this, Fna C2-treated mice had an increased number of intestinal adenomas and altered metabolites. Microbiome analysis of human tumour tissue from 116 patients with CRC demonstrated Fna C2 enrichment. Comparison of 62 paired specimens showed that only Fna C2 is tumour enriched compared to normal adjacent tissue. This was further supported by metagenomic analysis of stool samples from 627 patients with CRC and 619 healthy individuals. Collectively, our results identify the Fna clade bifurcation, show that specifically Fna C2 drives the reported Fn enrichment in human CRC and reveal the genetic underpinnings of pathoadaptation of Fna C2 to the CRC niche.
Bacteria in polymicrobial habitats contend with a persistent barrage of competitors, often under rapidly changing environmental conditions
. The direct antagonism of competitor cells is thus an ...important bacterial survival strategy
. Towards this end, many bacterial species employ an arsenal of antimicrobial effectors with multiple activities; however, the benefits conferred by the simultaneous deployment of diverse toxins are unknown. Here we show that the multiple effectors delivered to competitor bacteria by the type VI secretion system (T6SS) of Pseudomonas aeruginosa display conditional efficacy and act synergistically. One of these effectors, Tse4, is most active in high-salinity environments and synergizes with effectors that degrade the cell wall or inactivate intracellular electron carriers. We find Tse4 synergizes with these disparate mechanisms by forming pores that disrupt the ΔΨ component of the proton motive force. Our results provide evidence that the concomitant delivery of a cocktail of effectors serves as a bet-hedging strategy to promote bacterial competitiveness in the face of unpredictable and variable environmental conditions.
We report the complete genome sequence of Morganella morganii CTX51T, a strain isolated from the resected tumor of a patient with cecal colorectal adenocarcinoma of the cecum. The genome comprises a ...circular chromosome of 4.19 Mbp, with an overall GC content of 50.4% and one circular plasmid of 8.48 kbp.
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