A long‐term daily streamflow data set, covering the period from 1925 to 2013, was compiled for the Lena River basin, with an average record length of 49 years. Time series of mean annual daily flow ...(MADF) and extreme (maximum, Qmax, and minimum, Qmin) daily flows were subject to trend detection and change point detection analysis. Significant changes result mostly from rapid “breakpoint” homogeneity disruptions. Thirty one time series showed trends in MADF, 10, in Qmax, and 32 of the 55 records with nonzero Qmin, significant at p ≤ 0.05. Upward trends prevail in both mean annual and extreme flows, with average magnitudes of 47% (MADF), 56% (Qmax), and 68% (Qmin). Two to three stations in each subset showed downward trends (averaging −20%, −39%, and −38%, respectively). Abrupt changes are observed in the 1990s and early 2000s mostly in the headwaters of the basin, underlain by discontinuous permafrost.
Key Points
Upward trends are detected in mean annual and minimum flows in the Lena River basin
The Lena River discharge at the outlet increased by 15.6%, or 75 km3/yr, from 1936 to 2013
Abrupt changes in river flows are observed since the mid‐1990s in the southern part of the Lena basin
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•Molecular docking study of 3 macrocyclic compounds with QRF10 of SARS-CoV2 was shown.•Protoporphyrin and Fe(III)protoporphyrin form several types of complexes with the ORF10 ...protein.•The results of theoretical studies were confirmed by spectral data (UV–Vis, IR spectroscopy)•Chlorin forms with ORF10 more stable complexes than protoporphyrins.
The coronavirus disease 2019 (COVID-19) caused by the SARS-CoV-2 coronavirus has spread rapidly around the world in a matter of weeks. Most of the current recommendations developed for the use of antivirals in COVID-19 were developed during the initial waves of the pandemic, when resources were limited and administrative or pragmatic criteria took precedence. The choice of drugs for the treatment of COVID-19 was carried out from drugs approved for medical use. COVID-19 is a serious public health problem and the search for drugs that can relieve the disease in infected patients at various stages is still necessary. Therefore, the search for effective drugs with inhibitory and/or virucidal activity is a paramount task. Accessory proteins of the virus play a significant role in the pathogenesis of the disease, as they modulate the host's immune response. This paper studied the interaction of one of the SARS-CoV-2 accessory proteins ORF10 with macroheterocyclic compounds – protoporphyrin IX d.m.e., Fe(III)protoporphyrin d.m.e. and 5,10,15,20-tetrakis(3′-pyridyl)chlorin tetraiodide, which are potential inhibitors and virucidal agents. The SARS-CoV-2 ORF10 protein shows the highest affinity for Chlorin, which binds hydrophobically to the alpha structured region of the protein. Protoporphyrin is able to form several complexes with ORF10 close in energy, with alpha- and beta-molecular recognition features, while Fe(III)protoporphyrin forms complexes with the orientation of the porphyrin macrocycle parallel to the ORF10 alpha-helix. Taking into account the nature of the interaction with ORF10, it has been suggested that Chlorin may have virucidal activity upon photoexposure. The SARS-CoV-2 ORF10 protein was expressed in Escherichia coli cells, macroheterocyclic compounds were synthesized, and the structure was confirmed. The interaction between macrocycles with ORF10 was studied by spectral methods. The results of in silico studies were confirmed by experimental data.
Complex formation processes of tetrasulfosubstituted cobalt(II) phthalocyanine with ORF3a accessory protein of SARS-CoV-2 coronavirus were studied. The interaction of ORF3a protein with SARS-CoV-2 ...virus with tetrasulfosubstituted cobalt(II) phthalocyanine affords a stable complex in which metallophthalocyanine exists in the monomeric form. The complex formation induces slight changes in the secondary structure of the protein by increasing the fraction of disordered fragments of the polypeptide chain. The photoirradiation of the complex of ORF3a protein of SARS-CoV-2 virus with tetrasulfosubstituted cobalt(II) phthalocyanine leads to the photooxidation of amino acid residues of the protein.
The article is devoted to an analysis of some aspects, professional as well as creative ones, of the biography of Mathilde Hollitscher, Sigmund Freud’s eldest daughter. In this context her life story ...is presented as a point of convergence of several research trajectories, including the history of the psychoanalytic movement and biographical studies related to the founder of psychoanalysis and his relationship with the world of clothing and textiles. At the same time, this research focuses on the shift of the practices of women’s creative work from the invisible area of domestic labour to the sphere of professional activity implemented in the public space in the 20th century. The article considers the professional life of Freud’s eldest daughter as an illustrative example of such deterritorialization, and also outlines the connections of the Freud family with the world of textiles and clothing. All this opens up new possibilities to elucidate a number of episodes in the history of the psychoanalytic movement, as well as some facts of Sigmund Freud’s biography. The presented materials also create additional perspectives for comprehending the legacy of the founder of psychoanalysis.
The peculiarities of
COOLAIR
,
COLDAIR,
and
COLDWRAP
lncRNA expression, which perform an important function in the vernalization-mediated epigenetic mechanism of the transition to flowering in
A. ...thaliana
plants of northern natural populations (Karelia), are revealed. The results obtained differ in part from the data of other authors performing studies on pure lines and other ecotypes of this species. It is suggested that the genetic and epigenetic mechanisms involved in the process of vernalization and the control of flowering times may differ in plant populations from different geographic regions.
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