A computational analysis tool was used to characterize the flow structure in an S-shaped intake to determine the relationship between inlet shape and angle of incidence and to study the reduction of ...flow distortion on the engine face. A Royal Aircraft Establishment M 2129 S-shaped intake was used to establish the reliability of the proposed computational analysis technique. On the basis of previous research, the k-ω shear stress transport model for turbulence was used, and reliable results were obtained for complex flow structures, such as the secondary flow caused by adverse pressure gradient. The size of owl face separation and the angle of incidence at which the separation occurred differed according to the relation between the angle of incidence of the flow and the inlet shape. The size of the owl face separation was small in the S-shaped intake with an inlet shape close to that of an upper semicircle. Flow separation was delayed with respect to the angle of incidence.
This study presents a swarm-control algorithm to overcome the limitations inherent to single-object systems. The leader–follower swarm-control method was selected for its ease of mathematical ...interpretation and theoretical potential for the unlimited expansion of followers. However, a known drawback of this method is the risk of swarm collapse when the leader breaks down. To address this, a fault-coping algorithm was developed and supplemented to the leader–follower swarm-control method, which enabled the detection and responsive handling of failures, thereby ensuring mission continuity. Comprehensive data, including voltage, current, thruster speed, position, and heading angle were acquired and analyzed using sensors on unmanned surface vehicles (USVs) to monitor potential failures. In the case of a failure, such as thruster malfunction, the nearest USV seamlessly takes charge of the mission under the guidance of the fault-coping algorithm. The leader–follower swarm-control and fault-coping algorithms were successfully validated through actual sea area tests, which confirmed their operational efficacy. This study affirms the well-formed nature of the USV swarm formation and demonstrates the effectiveness of the fault-coping algorithm in ensuring normal mission performance under the virtual failure scenarios applied to the leader USV.
The phytohormone abscisic acid (ABA) plays crucial roles in various physiological processes, including responses to abiotic stresses, in plants. Recently, multiple ABA transporters were identified. ...The loss-of-function and gain-of-function mutants of these transporters show altered ABA sensitivity and stomata regulation, highlighting the importance of ABA transporters in ABA-mediated processes. However, how the activity of these transporters is regulated remains elusive. Here, we show that spatial regulation of ATP BINDING CASETTE G25 (ABCG25), an ABA exporter, is an important mechanism controlling its activity. ABCG25, as a soluble green fluorescent protein (sGFP) fusion, was subject to posttranslational regulation via clathrin-dependent and adaptor protein complex-2-dependent endocytosis followed by trafficking to the vacuole. The levels of sGFP:ABCG25 at the plasma membrane (PM) were regulated by abiotic stresses and exogenously applied ABA; PM-localized sGFP:ABCG25 decreased under abiotic stress conditions via activation of endocytosis in an ABA-independent manner, but increased upon application of exogenous ABA via activation of recycling from early endosomes in an ABA-dependent manner. Based on these findings, we propose that the spatial regulation of ABCG25 is an important component of the mechanism by which plants fine-tune cellular ABA levels according to cellular and environmental conditions.
Lab-on-a-CD (LOCD) is gaining importance as a diagnostic platform due to being low-cost, easy-to-use, and portable. During LOCD usage, mixing and reaction are two processes that play an essential ...role in biochemical applications such as point-of-care diagnosis. In this paper, we numerically and experimentally investigate the effects of the Coriolis and Euler forces in the mixing chamber during the acceleration and deceleration of a rotating disk. The mixing performance is investigated under various conditions that have not been reported, such as rotational condition, chamber aspect ratio at a constant volume, and obstacle arrangement in the chamber. During disk acceleration and deceleration, the Euler force difference in the radial direction causes rotating flows, while the Coriolis force induces perpendicular vortices. Increasing the maximum rotational velocity improves the maximum rotational displacement, resulting in better mixing performance. A longer rotational period increases the interfacial area between solutions and enhances mixing. Mixing performance also improves when there is a substantial difference between Euler forces at the inner and outer radii. Furthermore, adding obstacles in the angular direction also passively promotes or inhibits mixing by configuration. This quantitative investigation provides valuable information for designing and developing high throughput and multiplexed point-of-care LOCDs.
Endocytosis and subsequent trafficking pathways are crucial for regulating the activity of plasma membrane-localized proteins. Depending on cellular and physiological conditions, the internalized ...cargoes are sorted at (and transported from) the trans-Golgi network/early endosome (TGN/EE) to the vacuole for degradation or recycled back to the plasma membrane. How this occurs at the molecular level remains largely elusive. Here, we provide evidence that the ENTH domain-containing protein AtECA4 plays a crucial role in recycling cargoes from the TGN/EE to the plasma membrane in Arabidopsis thaliana. AtECA4:sGFP primarily localized to the TGN/EE and plasma membrane (at low levels). Upon NaCl or mannitol treatment, AtECA4:sGFP accumulated at the TGN/EE at an early time point but was released from the TGN/EE to the cytosol at later time points. The ateca4 mutant showed higher resistance to osmotic stress and more sensitive to exogenous abscisic acid (ABA) than the wild type, as well as increased expression of ABA-inducible genes RD29A and RD29B. Consistently, ABCG25, a plasma membrane-localized ABA exporter, accumulated at the prevacuolar compartment in ateca4, indicating a defect in recycling to the plasma membrane. However, the role of AtECA4 in cargo recycling is not specific to ABCG25, as it also functions in the recycling of BRI1. These results suggest that AtECA4 plays a crucial role in the recycling of endocytosed cargoes from the TGN/EE to the plasma membrane.
AtECA4 primarily localizes to the TGN/EE and plays a role in recycling of proteins from the TGN/EE to the plasma membrane. This AtECA4-mediated recycling of cargo proteins ABCG25 and BRI1 is crucial for homeostasis of cellular ABA levels and brassinolide-mediated signaling for plant growth.
Vascular access (VA), a renal failure therapy, is often performed using an arteriovenous (AV) graft for patients with veins and arteries that cannot be connected with autologous blood vessels. ...However, VA using AV grafts can change the blood flow and lead to intimal hyperplasia (IH), causing the damage on blood vessel and failure of VA. In our study, we investigated the effect of the anastomosis angle on blood vessel damage under various IH formation conditions. We simulated the blood flow near the anastomosis between vein and AV graft and quantitatively evaluated the blood vessel damage using hemodynamic factors, such as wall shear stress (WSS). Our results show that smaller anastomosis angle reduces damage to blood vessels and prevents IH formation and growth regardless of IH progression, shape, and position. These results can contribute to optimization of the anastomosis angle during VA surgery to improve a patient’s prognosis.
Polymer-based lab-on-a-disc (LoaD) devices for isolating ribonucleic acid (RNA) from whole blood samples have gained considerable attention for accurate biomedical analysis and point-of-care ...diagnostics. However, the mass production of these devices remains challenging in manufacturing cost and sustainability, primarily due to the utilization of a laser cutter or router computer numerical control (CNC) machine for engraving and cutting plastics in the conventional prototyping process. Herein, we reported the first energy-efficient room-temperature printing-imprinting integrated roll-to-roll manufacturing platform for mass production of a polydimethylsiloxane (PDMS)-based LoaD to on-site isolate ribonucleic acid (RNA) from undiluted blood samples. We significantly reduced energy consumption and eliminated thermal expansion variations between the mold, substrate, and resists by accelerating the PDMS curing time to less than 10 min at room temperature without using heat or ultraviolet radiation. The additive manufacturing technology was applied to fabricate a multi-depth flexible polymer mold that integrated macro (2 mm) and micro-sized (500 μm) features, which overcomes the economic and environmental challenges of conventional molding techniques. Our integrated R2R platform was enabled to print adhesion-promoting films at the first printing unit and continuously in-line imprint with a high replication accuracy (99%) for high-volume manufacturing of a new centrifugal microfluidic chip with an enhancement of mixing performance by integrating an efficient mixing chamber and serpentine micromixer. This research paved the way for scalable green manufacturing of large-volume polymer-based microfluidic devices, often required in real-world sample-driven analytical systems for clinical bioanalysis.
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This study focuses on faults in the thrusters of unmanned surface vehicles, which are fatal to the integrity of their missions. As for the fault conditions, the breakage of the thruster blade and the ...entanglement of floating objects were selected, and a data-driven method was used to diagnose the faults. In the data-driven method, it is important to select the sensitive fault feature. In this study, vibration, current consumption, rotational speed and input voltage were selected as fault features. An experiment was conducted in an engineering water tank to obtain and analyze data on fault conditions to verify the validity of the selected features. In addition, a new fault diagnosis algorithm combining principal component analysis and Shannon entropy was applied for analyzing the correlations among fault features. This algorithm reduces the dimensionality of data while preserving their structure and characteristics, and diagnoses faults by quantifying entropy values. A fault is detected by comparing the entropy value and a predetermined threshold value, and is diagnosed by analyzing the entropy value and visualized 2D or 3D principal component results. Moreover, the fault diagnosis performance of the unmanned surface vehicle’s thruster was verified by analyzing the results for each fault condition.
The current study aims to understand the aero-thermal performance of a cooled cavity tip in a single stage transonic turbine. The squealer tip of the uncooled turbine blade was reduced to an ...aerodynamic loss with suppressing leakage flow. However, the aerodynamic loss study of the cooled turbine blade tip is rare. It is necessary to study the tip cavity of the cooled turbine blade. Depth, front blend radius and aft blend radius of the cavity were set as design variables, and 30 cases were chosen using design of experiments. These cases were calculated with conjugate heat transfer method. Approximation model was made using the Kriging method, and tip cavity shape was optimized with multidisciplinary design optimization. Average total pressure loss behind the trailing edge and cooling effectiveness of blade tip surface were set to the objective function. The aerodynamic optimization model decreased 1.6 % of total pressure loss, the heat transfer optimization model increased 1.3 % point of cooling effectiveness and aero-thermal optimization model were found. Volume of tip cavity becomes larger when three design variables are grown. Amount of tip leakage flow and its distribution over the tip region increases and total pressure loss and cooling effectiveness increase. In terms of heat transfer, blade tip without cavity is advantageous. Total pressure loss coefficient, however, also increases over 5 %. To improve both aero-thermal characteristics of cooled blade tip, the design using the multidisciplinary design optimization is recommended.
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