Introduction: The COVID-19 epidemic and its restrictions have affected all aspects of people’s lives, including health-related quality of life and, considering sexuality as an integral part of ...individual needs, also intimacy and sexuality. Therefore, the aim of this article was to investigate women’s sexual functioning and health-related quality of life assessment in Slovenian women in the reproductive period. Methods: An online survey with valid questionnaires (short form 36 SF-36 and Female sexual function index FSFI-19) was conducted in January 2022. All research ethical measures were taken to ensure the integrity of the participants. Results: The FSFI scale score was 25.37 ± 8.29, 1.18 points above the cutoff point, indicating a higher risk of sexual dysfunction (26.55). The estimated prevalence of sexual dysfunction was 36.8%, with sexual desire being the most problematic area. The mean score on the SF-36 scale in our sample reached 73.52 ± 13.84 on a 100-point scale, with 0 representing the worst and 100 the best quality of life; fatigue (x̅ = 48.50) was the most problematic category. The results partly reflect the results of foreign studies, but we must be aware of cultural differences in the understanding of sexuality and keep in mind that some countries faced difficult circumstances during the COVID-19 pandemic and that social constraints were different in 2020 than in 2022. Conclusion: The epidemic affected the sexual function of women of reproductive age and their perception of (health-related) quality of life.
Necrosis and ethylene-inducing peptide 1–like (NLP) proteins constitute a superfamily of proteins produced by plant pathogenic bacteria, fungi, and oomycetes. Many NLPs are cytotoxins that facilitate ...microbial infection of eudicot, but not of monocot plants. Here, we report glycosylinositol phosphorylceramide (GIPC) sphingolipids as NLP toxin receptors. Plant mutants with altered GIPC composition were more resistant to NLP toxins. Binding studies and x-ray crystallography showed that NLPs form complexes with terminal monomeric hexose moieties of GIPCs that result in conformational changes within the toxin. Insensitivity to NLP cytolysins of monocot plants may be explained by the length of the GIPC head group and the architecture of the NLP sugar-binding site. We unveil early steps in NLP cytolysin action that determine plant clade-specific toxin selectivity.
Necrosis and ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs) are secreted by several phytopathogenic microorganisms. They trigger necrosis in various eudicot plants upon binding to plant ...sphingolipid glycosylinositol phosphorylceramides (GIPC). Interestingly, HaNLP3 from the obligate biotroph oomycete Hyaloperonospora arabidopsidis does not induce necrosis. We determined the crystal structure of HaNLP3 and showed that it adopts the NLP fold. However, the conformations of the loops surrounding the GIPC headgroup-binding cavity differ from those of cytotoxic Pythium aphanidermatum NLPPya. Essential dynamics extracted from μs-long molecular dynamics (MD) simulations reveals a limited conformational plasticity of the GIPC-binding cavity in HaNLP3 relative to toxic NLPs. This likely precludes HaNLP3 binding to GIPCs, which is the underlying reason for the lack of toxicity. This study reveals that mutations at key protein regions cause a switch between non-toxic and toxic phenotypes within the same protein scaffold. Altogether, these data provide evidence that protein flexibility is a distinguishing trait of toxic NLPs and highlight structural determinants for a potential functional diversification of non-toxic NLPs utilized by biotrophic plant pathogens.
The lack of efficient methods to control the major diseases of crops most important to agriculture leads to huge economic losses and seriously threatens global food security. Many of the most ...important microbial plant pathogens, including bacteria, fungi, and oomycetes, secrete necrosis- and ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs), which critically contribute to the virulence and spread of the disease. NLPs are cytotoxic to eudicot plants, as they disturb the plant plasma membrane by binding to specific plant membrane sphingolipid receptors. Their pivotal role in plant infection and broad taxonomic distribution makes NLPs a promising target for the development of novel phytopharmaceutical compounds. To identify compounds that bind to NLPs from the oomycetes Pythium aphanidermatum and Phytophthora parasitica, a library of 587 small molecules, most of which are commercially unavailable, was screened by surface plasmon resonance. Importantly, compounds that exhibited the highest affinity to NLPs were also found to inhibit NLP-mediated necrosis in tobacco leaves and Phytophthora infestans growth on potato leaves. Saturation transfer difference-nuclear magnetic resonance and molecular modelling of the most promising compound, anthranilic acid derivative, confirmed stable binding to the NLP protein, which resulted in decreased necrotic activity and reduced ion leakage from tobacco leaves. We, therefore, confirmed that NLPs are an appealing target for the development of novel phytopharmaceutical agents and strategies, which aim to directly interfere with the function of these major microbial virulence factors. The compounds identified in this study represent lead structures for further optimization and antimicrobial product development.
3 OPISTESTNEGA PROJEKTA RAF V letu 2022 smo na Gursu podprli izvedbo projekta »Poševno aero fotografiranj e - test«, katerega predmet naročila je bila izvedba poševnega aerofotografiranja (PAF) za ...pridobitev aerofotografij treh različnih geometrijskih ločljivosti na treh različnih testnih lokacijah v Sloveniji. Rezultat zajema aerofotografij in GNSS/ INS meritev so: natančni parametri zunanje orientacije aerofotografij, digitalni model površja (DMP), popolni ortofoto (POF), 3D mreža (angl. 3D meshY izdelana pa je bila tudi aplikacija za pregledovanje in izvajanje osnovnih meritev na poševnih aerofotografijah. Fotografiranje Ljubljane se je izvajalo z različico PAS880İ, ki ima v sistem dodan tudi NIR fotoaparat z ločljivostjo 150 MP. Sistem za zajem fotografij je bil nameščen na letalo, kot je prikazano na sliki 3, kjer je tudi skica smeri in oblike zajetih fotografij. 4 IZDELKI TESTNEGA PROJEKTA Uporabniku naj zanimivejši izdelki testnega projekta so: orientirane poševne aero fotografij e, digitalni model površja, popolni ortofoto, 3D mreža ter aplikacija za pregledovanje in 3D merjenje. S slike jerazvidno, da poševne aerofortografije ponujajo veliko boljšo prostorsko in semantično predstavo o izbrani stavbi, kot bi jo omogočale zgolj nadirne aerofotografije. 4.2 Digitalni model višin (DMV) Za prikaz višinskih podatkov površja Zemlje običajno uporabljamo digitalni model višin (DMV), ki se deli na dva tipa: digitalni model reliefa (DMR), ki modelira teren, in digitalni model površja (DMP), ki modelira površje.
Equistatin is a protein composed of three thyroglobulin type-1 domains. It inhibits papain-like cysteine proteinases and the aspartic proteinase, cathepsin D. To determine the structural basis for ...this inhibition we cloned and expressed the separated domains (eq d-1, eq d-2, eq d-3) in
Pichia pastoris. Kinetic constants for the interaction of eq d-1 with papain and that of eq d-2 with cathepsin D are of similar order (subnanomolar) and are comparable to the constants obtained for full-length equistatin. The target proteinase for the third domain remains unknown. Thus, we demonstrate here that thyroglobulin type-1 motifs per se are able to support specific structural features that enable them to inhibit proteases from different classes. The overall conformation of three domains in equistatin is such that the interaction of domains 1 or 2 with their respective target enzymes is not hindered sterically by either domain. In addition, we show that the interaction of eq d-2 with cathepsin D results in conformational changes, which is not the case for the eq d-1/papain interaction.
Equistatin is a 199-residue protein composed of three thyroglobulin type-1 domains. It strongly inhibits cysteine proteinases as well as the aspartic proteinase cathepsin D. In order to initiate ...structure-function studies by protein engineering, a cDNA library from sea anemone, Actinia equina, was screened. A positive clone of 888 nucleotides was shown to encode a protein of 231 amino acids, including the signal sequence. The mature protein region was amplified by PCR, cloned into the pET22b(+)cas expression vector and expressed in Escherichia coli. Isolation of active recombinant equistatin required only one purification step, the His-tag affinity column. The protein displays physical and inhibitory properties closely similar to the native inhibitor.
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