A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterial strain, designated 16F3Y-2
T
, was isolated from the Han River, South Korea, and was characterized taxonomically ...using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 16F3Y-2
T
belonged to the family
Cytophagaceae
in the phylum
Bacteroidetes
and was most closely related to ‘
Hymenobacter terrae
’ DG7A (98.01%),
H. soli
PB17
T
(97.26%),
H. glaciei
VUG-A130
T
(96.78%),
H. antarcticus
VUG-A42aa
T
(96.72%),
H. ruber
PB156
T
(96.61%), and
H. saemangeumensis
GSR0100
T
(95.77%). The G+C content of the genomic DNA of strain 16F3Y-2
T
was 62.9 mol%. The isolate contained MK-7 as the predominant respiratory quinone, and summed feature 3 (C
16:1
ω
7
c
/C
16:1
ω
6
c
; 35.5%), C
15:0
iso (16.9%), C
16:1
ω
5
c
(10.9%), and C
15:0
anteiso (9.9%) as major fatty acids. The major polar lipid was phosphatidylethanolamine. Phenotypic and chemotaxonomic data supported the affiliation of strain 16F3Y-2
T
with the genus
Hymenobacter
. However, strain 16F3Y-2
T
exhibited relatively low levels of DNA-DNA relatedness with ‘
H. terrae
’ KCTC 32554 (44.1%) and
H. soli
KCTC 12607
T
(24.3%), clearly indicating that the isolate constitutes a new genospecies. Strain 16F3Y-2
T
could be differentiated from its phylogenetic neighbors on the basis of several phenotypic, genotypic, and chemotaxonomic features. Therefore, strain 16F3Y-2
T
represents a novel species in the genus
Hymenobacter
, for which the name
Hymenobacter daeguensis
sp. nov. is proposed. The type strain is 16F3Y-2
T
(=KCTC 52537
T
=JCM 31654
T
).
A Gram-negative, short rod-shaped, and pink-pigmented bacterial strain, designated MA1
T
, was isolated from a soil sample from Gijang-gun, Busan in Republic of Korea. The 16S rRNA gene sequence ...analysis showed that strain MA1
T
belonged to the genus
Larkinella
and was closely related to “
Larkinella punicea
” (97.5% similarity),
Larkinella rosea
15J16-1T3A
T
(96.5%), and
Larkinella knui
15J6-3T6
T
(96.2%). Polar lipid profile of strain MA1
T
contained phosphatidylethanolamine, two unidentified aminolipids, and three unidentified lipids. Menaquinone-7 was the only quinone and the main fatty acids were C
16:1
ω
5c (36.7%), iso-C
15:0
(30.0%), iso-C
17:0
3-OH (7.7%), and summed feature 3 (C
16:1
ω
6c and/or C
16:1
ω
7c and/or iso-C
15:0
2-OH) (7.3%). The genomic DNA G + C content was 52.3 mol% based on the whole-genome analysis. Strain MA1
T
exhibited a relatively low level of ANI and in silico DDH values with “
Larkinella punicea
” (91.9 and 47.1%, respectively),
Larkinella rosea
(79.7 and 23.3%), and
Larkinella knui
(
81.9 and 25.7%). Based on its phenotypic properties and phylogenetic distinctiveness, strain MA1
T
should be classified in the genus
Larkinella
as a representative of a novel species, for which the name
Larkinella humicola
sp. nov. is proposed. The type strain is MA1
T
(= KCTC 72629
T
= NBRC 114191
T
).
The taxonomic position of bacterial strain, designated 15J16-1T3A
T
, recovered from a soil sample was established using a polyphasic approach. Phylogenic analysis based on the 16S rRNA gene sequence ...showed that strain 15J16-1T3A
T
belonged to the family
Cytophagaceae
, phylum
Bacteroidetes
, and was most closely related to ‘
Larkinella harenae
’ 15J9-9 (95.9% similarity),
Larkinella ripae
15J11-11
T
(95.6%),
Larkinella bovis
M2TB15
T
(94.7%),
Larkinella arboricola
Z0532
T
(93.9%), and
Larkinella insperata
LMG 22510
T
(93.5%). Cells were rod-shaped, Gram-stain-negative, aerobic, and nonmotile. The isolate grew on NA, R2A, TSA, but not on LB agar. The strain was able to grow at temperature range from 10°C to 30°C with an optimum at 25°C and pH 6–8. Menaquinone MK-7 was the predominant respiratory quinone. The major cellular fatty acids comprised C
16:1
ω5c
(48.6%) and C
15:0
iso (24.1%). Phosphatidylethanolamine, phosphatidylserine, and an unidentified lipid were the major polar lipids. The G + C content of the genomic DNA was 49.5 mol%. Strain 15J16-1T3A
T
could be distinguished from its closest phylogenetic neighbors based on its phenotypic, genotypic, and chemotaxonomic features. Therefore, the isolate is considered to represent a novel species in the genus
Larkinella
, for which the name
Larkinella roseus
sp. nov. is proposed. The type strain is 15J16-1T3A
T
(= KCTC 52004
T
= JCM 31991
T
).
Spirosoma panaciterrae sp. nov., isolated from soil Ten, Leonid N; Xu, Jin-Li; Jin, Feng-Xie ...
International journal of systematic and evolutionary microbiology,
02/2009, Letnik:
59, Številka:
Pt 2
Journal Article
Recenzirano
Odprti dostop
A Gram-negative, yellowish bacterial strain, designated Gsoil 1519(T), was isolated from soil of a ginseng field in Pocheon province (South Korea) and characterized using a polyphasic approach to ...determine its taxonomic position. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 1519(T) belongs to the family 'Flexibacteraceae' and is related to Spirosoma rigui KCTC 12531(T) (91.8 % similarity) and Spirosoma linguale LMG 10896(T) (91.5 % similarity). Phylogenetic distances from any other recognized species within the family 'Flexibacteraceae' were greater than 14.7 %. The G+C content of the genomic DNA of strain Gsoil 1519(T) was 50.1 %. The detection of MK-7 as the predominant menaquinone and a fatty acid profile with C(16 : 1)omega5c, summed feature 4 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH), iso-C(15 : 0) and C(16 : 0) as the major acids supported the affiliation of strain Gsoil 1519(T) to the genus Spirosoma. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 1519(T) should be classified in the genus Spirosoma as a representative of a novel species, for which the name Spirosoma panaciterrae sp. nov. is proposed. The type strain is Gsoil 1519(T) (=KCTC 22263(T)=DSM 21099(T)).
A Gram-stain-negative, non-motile, non-spore-forming, rodshaped, aerobic bacterial strain, designated S1-2-2-5
T
, was isolated from the Jeollabuk-do province, Republic of Korea, and was ...characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain S1-2-2-5
T
belonged to the family
Cytophagaceae
in phylum
Bacteroidetes
, and was most closely related to
Hymenobacter terrae
DG7A
T
(98.2%),
Hymenobacter rubidus
DG7B
T
(98.0%),
Hymenobacter soli
PB17
T
(97.7%),
Hymenobacter daeguensis
16F3Y-2
T
(97.2%) and
Hymenobacter saemangeumensis
GSR0100
T
(97.0%). The G + C content of the genomic DNA of strain S1-2-2-5
T
was 59.4 mol%. The detection of menaquinone MK-7 as the predominant respiratory quinone, a fatty acid profile with summed feature 3 (C
16:1
ω
7
c
/C
16:1
ω
6
c
; 32.0%), C
15:0
iso (19.0%), and C
15:0
anteiso (15.0%) as the major components, and a polar lipid profile with phosphatidylethanolamine as the major component supported the affiliation of strain S1-2-2-5
T
to the genus
Hymenobacter
. The DNA-DNA relatedness between strain S1-2-2-5
T
and
H. terrae
KCTC 32554
T
,
H. rubidus
KCTC 32553
T
,
H. soli
KCTC 12607
T
,
H. daeguensis
KCTC 52537
T
, and
H. saemangeumensis
KACC 16452
T
were 49.5, 48.2, 34.1, 28.1, and 31.8% respectively, clearly showing that the isolate is not related to them at the species level. Strain S1-2-2-5
T
could be clearly differentiated from its closest neighbors on the basis of its phenotypic, genotypic and chemotaxonomic features. Therefore, strain S1-2-2-5
T
represents a novel species of the genus
Hymenobacter
, for which the name
Hymenobacter terrigena
sp. nov. is proposed. The type strain is S1-2-2-5
T
(= KCTC 52737
T
= JCM 32195
T
).
A Gram-stain negative, rod shaped, motile by gliding, yellow-pigmented, aerobic bacterium, designated SE-1-e
T
, was isolated from a soil sample collected on Dokdo Island, South Korea. The isolate ...was characterized taxonomically using a polyphasic approach based on the phenotypic and genomic analyses. The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SE-1-e
T
belonged to the genus
Flavobacterium
in the family
Flavobacteriaceae
and had the highest sequence similarity with
Flavobacterium cheongpyeongense
IMCC34759
T
(97.5%),
Flavobacterium arsenitoxidans
S2-3H
T
(97.4%),
Flavobacterium resistens
BD-b365
T
(97.4%), and
Flavobacterium chungangense
CJ7
T
(97.4%). The predominant respiratory quinone of the isolate was found to be MK-6; the main polar lipid was phosphatidylethanolamine; and the major fatty acids were identified as summed feature 3 (C
16:1
ω
7
c
/C
16:1
ω
6
c
), C
15:0
iso, and C
16:0
. The draft genome of strain SE-1-e
T
had a length of 3,715,609 bp and a DNA G + C content of 34.8 mol%. The nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the novel isolate and
F. cheongpyeongense
IMCC34759
T
,
F
.
resistens
BD-b365
T
, and
Flavobacterium chungangense
CJ7
T
ranged from 74.9 to 75.3% and from 20.2 to 20.9%, respectively. On the basis of its phenotypic properties, genotypic distinctiveness, and chemotaxonomic features, strain SE-1-e
T
represents a novel species in the genus
Flavobacterium
, for which the name
Flavobacterium agrisoli
sp. nov. is proposed. The type strain is SE-1-e
T
(= KCTC 82352
T
= JCM 34302
T
).
A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J9-6
T
, was isolated from beach soil on Jeju Island, South Korea. Strain 15J9-6
T
, grew at 10–30°C ...(optimum growth at 25°C) and pH 7–8 (optimum growth at pH 7) on R2A, NA, and TSA agar. Phylogenetically, the strain was closely related to members of the genus
Spirosoma
(92.3–90.1% 16S rRNA gene sequence similarities) and showed highest sequence similarity to
Spirosoma panaciterrae
DSM 21099
T
(92.3%). The G+C content of the genomic DNA of strain 15J9-6
T
was 45.7 mol%. The strain contained phosphatidylethanolamine, two unidentified aminophospholipids, an unidentified phospholipid, and an unidentified lipid as the major polar lipids; menaquinone MK-7 as the predominant respiratory quinone and summed feature 3 (C
16:1
ω
6
c
/C
16:1
ω
7
c
; 30.1%), C
16:1
ω
5
c
(23.1%), iso C
15:0
(13.3%), and C
16:0
(8.4%) as the major fatty acids which supported the affiliation of strain 15J9-6
T
to the genus
Spirosoma
. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 15J9-6
T
from recognized
Spirosoma
species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain 15J9-6
T
represents a novel species of the genus
Spirosoma
, for which the name
Spirosoma daeguensis
sp. nov. is proposed. The type strain is 15J9-6
T
(=KCTC 52036
T
=JCM 31995T)
A novel, Gram-staining negative, yellow pigmented bacterial strain, designated 15J11-2
T
, was isolated from soil sample on Jeju Island, Republic of Korea. The strain was subjected to a taxonomic ...study using a polyphasic approach. The strain was able to grow at temperature range from 10°C to 30°C, pH 7–8, and in presence of 0–1% (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain 15J11-2
T
belongs to the genus
Spirosoma
and levels of 16S rRNA gene sequence similarity ranged from 91.5% to 89.8%. The genomic DNA G + C content of strain 15J11-2
T
was 46.0 mol%. The isolate contained phosphatidylethanolamine and an unidentified aminophospholipid as the main polar lipids, menaquinone MK-7 as the predominant respiratory quinone, and summed feature 3 (C
16:1
ω
6
c
/C
16:1
ω
7
c
; 39.4%), C
16:1
ω
5
c
(27.1%), and C
16:0
(13.0%) as the major fatty acids, which supported the affiliation of strain 15J11-2
T
to the genus
Spirosoma
. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 15J11-2
T
from recognized
Spirosoma
species. On the basis of its phenotypic properties, genotypic distinctiveness, chemotaxonomic features, strain 15J11-2
T
represents a novel species of the genus
Spirosoma
, for which the name
Spirosoma flavus
sp. nov. is proposed. The type strain is 15J11-2
T
(= KCTC 52026
T
= JCM 31998
T
).