Although SARS-CoV-2 primarily targets the respiratory system, patients with and survivors of COVID-19 can suffer neurological symptoms
. However, an unbiased understanding of the cellular and ...molecular processes that are affected in the brains of patients with COVID-19 is missing. Here we profile 65,309 single-nucleus transcriptomes from 30 frontal cortex and choroid plexus samples across 14 control individuals (including 1 patient with terminal influenza) and 8 patients with COVID-19. Although our systematic analysis yields no molecular traces of SARS-CoV-2 in the brain, we observe broad cellular perturbations indicating that barrier cells of the choroid plexus sense and relay peripheral inflammation into the brain and show that peripheral T cells infiltrate the parenchyma. We discover microglia and astrocyte subpopulations associated with COVID-19 that share features with pathological cell states that have previously been reported in human neurodegenerative disease
. Synaptic signalling of upper-layer excitatory neurons-which are evolutionarily expanded in humans
and linked to cognitive function
-is preferentially affected in COVID-19. Across cell types, perturbations associated with COVID-19 overlap with those found in chronic brain disorders and reside in genetic variants associated with cognition, schizophrenia and depression. Our findings and public dataset provide a molecular framework to understand current observations of COVID-19-related neurological disease, and any such disease that may emerge at a later date.
Ischemia/reperfusion injury (IRI) permeates a variety of diseases and is a ubiquitous concern in every transplantation proceeding, from whole organs to modest grafts. Given its significance, efforts ...to evade the damaging effects of both ischemia and reperfusion are abundant in the literature and they consist of several strategies, such as applying pre-ischemic conditioning protocols, improving protection from preservation solutions, thus providing extended cold ischemia time and so on. In this review, we describe many of the latest pharmacological approaches that have been proven effective against IRI, while also revisiting well-established concepts and presenting recent pathophysiological findings in this ever-expanding field. A plethora of promising protocols has emerged in the last few years. They have been showing exciting results regarding protection against IRI by employing drugs that engage several strategies, such as modulating cell-surviving pathways, evading oxidative damage, physically protecting cell membrane integrity, and enhancing cell energetics.
We report on recent advances in the use of inexpensive polymer optical fibres (POFs) for sensing applications in avionics. The sensors analysed in this manuscript take advantage of the unique ...properties of polymers, such as high flexibility, elasticity, and sensitivity, and they range from strain, elongation, and vibration interrogators to level and temperature meters, leading to cost-effective techniques for structural health monitoring in aircraft structures. We also highlight recent power-supply methods using Power-over-POF in order to feed sensors remotely, and we discuss the constraints imposed by connectors on the performance of POF networks in aircrafts.
Poor diet quality and obesity, especially abdominal obesity, have been associated with systemic inflammation. The neutrophil-to-lymphocyte Ratio (NLR) is an available and inexpensive inflammation ...biomarker. The aim of the present study was to determine the association of dietary patterns and obesity with an inflammatory state. A group of 1747 Spanish noninstitutionalized older adults individuals were included, and a food-frequency questionnaire was applied. The Global Food Score (GFS) and Healthy Eating Index for Spanish population (SHEI) were calculated. Weight, height and waist (WC) and hip circumferences were measured, and BMI, waist-to-height ratio (WHtR), and waist-to-hip ratio (WHR) determined. In addition, body-fat percentage was measured by bioimpedance. NLR was calculated (NLR ≥ p80: 2.6; 2.8 and 2.4 as inflammatory status in the entire population, men and women, respectively). The men with inflammatory status presented significative higher values of WC, WHtR, WHR, and body-fat percentage (101.82 ± 10.34 cm, 0.61 ± 0.06, 0.98 ± 0.06, and 31.68 ± 5.94%, respectively) than those with better inflammatory status (100.18 ± 10.22 cm, 0.59 ± 0.06, 0.97 ± 0.07, and 30.31 ± 6.16%, respectively). Those males with worse inflammatory state had lower scores for protein foods (OR = 0.898 (0.812-0.993);
= 0.037). The women with NLR ≥ 2.4 had higher WHtR and WHR (0.62 ± 0.09 and 0.91 ± 0.09) than those with NLR < 2.4 (0.60 ± 0.08 and 0.90 ± 0.08). In multiple linear regression analysis, NLR was positively related with WHtR and negatively related with SHEI score (β = 0.224 ± 0.094;
= 0.060;
< 0.05 and β = -0.218 ± 0.101;
= 0.061;
< 0.05), adjusting by sex, age, marital status, education level, smoking, hours of sleeping and inflammatory diseases. In women, the higher the SHEI and GFS scores were and the better meeting the aims of cereal and vegetable servings, the less the odds of inflammatory status (OR = 0.970 (0.948-0.992);
= 0.008; OR = 0.963 (0.932-0.995);
= 0.024; OR = 0.818 (0.688-0.974);
= 0.024 and OR = 0.829 (0.730-0.942);
= 0.004, respectively). WHtR and quality of diet is related to the inflammation status in older adults regardless to the sex.
This study analyzes tide transformation in the Guadalquivir estuary (SW Spain). When fresh water discharges are less than 40 m3/s, the estuary is tidally‐dominated (flood‐dominated) and well mixed. ...Under such conditions, the estuary can be divided into three stretches, each characterized by a different tide propagation process. In the first stretch of 25 km, the dominant process is diffusion. In the next stretch, approximately over 35 km length, convergence and friction processes are in balance. At the head of the estuary, in the last stretch, the tidal motion is partially standing because of tidal reflection on the Alcalá del Río dam, located 110 km upstream from the estuary mouth. The reflection coefficient R varies with the frequency; for diurnal constituents its magnitude ∣RD∣ is 0.25; this value increases in the case of semi‐diurnal (∣RS∣ ≈ 0.40), and quarter‐diurnal constituents (∣RQ∣ ≈ 0.65), and reaches its minimum at the sixth‐diurnal components (∣RX∣ ≈ 0.10). The tidal reflection can generate residual currents that have consequences in the bed morphology. Furthermore, when the fresh water discharges are greater than 400 m3/s, the estuary is fluvially‐dominated and the water level can be calculated as the linear superposition of tide and river contributions. However, superposition arguments do not hold for currents at any point in the estuary.
Key Points
The prominent tidal reflection at the head controls estuarine morphohydrodynamics
The estuary is divided into 3 stretches based on the dominant tidal processes
Three years of hydrodynamic data from 29 moorings were analyzed
Within the ovary, the obturator bridges the pathway of the pollen tube from the style to the ovule. Despite its widespread presence among flowering plants, its function has only been studied in a ...handful of species, and the molecules involved in pollen tube-obturator cross-talk have not been explored hitherto. This work evaluates the involvement of glucans and glycoproteins on pollen tube growth in the obturator of apple flowers ( Malus × domestica) .
Pollen tube kinetics were sequentially examined in the pistil and related to changes occurring on the obturator using histochemistry and inmunocytochemistry. To discriminate between changes in the obturator induced by pollen tubes from those developmentally regulated, both pollinated and unpollinated pistils were examined.
Pollen tube growth rates were slow in the stigma, faster in the style and slow again in the ovary. The arrival of pollen tubes at the obturator was concomitant with the secretion of proteins, saccharides and glycoprotein epitopes belonging to extensins and arabinogalactan proteins (AGPs). While some of these secretions - extensins and AGPs labelled by JIM13 - were developmentally regulated, others - AGPs labelled by JIM8 - were elicited by the presence of pollen tubes. Following pollen tube passage, all these glycoproteins were depleted.
The results show a timely secretion of glycoproteins on the obturator surface concomitant with pollen tube arrival at this structure. The fact that their secretion is depleted following pollen tube passage strongly suggests their role in regulating pollen tube access to the ovule. Remarkably, both the regulation of the secretion of the different glycoproteins, as well as their association with the performance of pollen tubes exhibit similarities with those observed in the stigma, in line with their common developmental origin.
This paper explores the use of a mixture model for determining the marginal distribution of hydrological variables, consisting of a truncated central distribution that is representative of the ...central or main‐mass regime, which for the cases studied is a lognormal distribution, and of two generalized Pareto distributions for the maximum and minimum regimes, representing the upper and lower tails, respectively. The thresholds defining the limits between these regimes and the central regime are parameters of the model and are calculated together with the remaining parameters by maximum likelihood. After testing the model with a simulation study we concluded that the upper threshold of the model can be used when applying the peak over threshold method. This will yield an automatic and objective identification of the threshold presenting an alternative to existing methods. The model was also applied to four hydrological data series: two mean daily flow series, the Thames at Kingston (United Kingdom), and the Guadalfeo River at Orgiva (Spain); and two daily precipitation series, Fort Collins (CO, USA), and Orgiva (Spain). It was observed that the model improved the fit of the data series with respect to the fit obtained with the lognormal (LN) and, in particular, provided a good fit for the upper tail. Moreover, we concluded that the proposed model is able to accommodate the entire range of values of some significant hydrological variables.
Key Points
A PDF for the entire range of values of some hydrological variables
Automatic selection of the threshold required for applying the POT method
Improved methodology for uncertainty estimation of the threshold
•The impact of Si application to grapevines on red wine composition has been examined.•Si application to grapevines leads to a less oxidized wine.•Wine made with grapes treated with silicon presents ...higher contents of phenolics.•Si application to grapevines may lead to wines with a more complex fruity aroma.
Impact of applying monosilicic acid to grapevines during ripening on chemical composition of Mencía red wines in an area where fungal diseases during summer are common was examined. The foliar application of monosilicic acid to grapevines led to a less oxidized wine, with lower levels of acetic acid, acetaldehyde, ethyl acetate and diacethyl; this should be considered as positive from a sensory point of view. Wines made with silicon-treated grapes also contained lower levels of gluconic acid and glycerol, which are chemical markers of wines made with botrytized grapes, as well as higher contents of total phenols, anthocyanins and tannins. Furthermore, the contents of several mid-chain alcohols were higher (p < 0.05) in wines made with grapes from silicon-treated plants.
We assessed the conservation priority of 18 freshwater ecoregions in southern South America on the basis of Aegla (genus of freshwater crabs) genetic diversity and distribution. Geographical ...distributions for 66 Aegla species were taken from the literature and plotted against ecoregions and main river basins of southern South America. Species richness and number of threatened and endemic species were calculated for each area. To assess taxonomic and phylogenetic diversity, we generated a molecular phylogeny based on DNA sequences for one nuclear (28S) and 4 mitochondrial (12S, 16S, COI, and COII) genes. All species richness and phylogenetic methods agreed, to a large extent, in their rankings of the importance of conservation areas, as indicated by the Spearman's rank correlation coefficient (p < 0.01); nonetheless, some of the lowest correlations were observed between taxonomic and phylogenetic diversity indices. The 5 ecoregions of the Laguna dos Patos Basin (Eastern Brazil), Central Chile, South Brazilian Coast, Chilean Lakes, and Subtropical Potamic Axis (northern Argentina and southern Uruguay and Paraguay) had the highest biodiversity scores. Conservation of these regions will preserve the largest number of species and the greatest amount of genetic diversity within the South American freshwater Aegla fauna. Biodiversity across rivers and within areas was heterogeneously distributed in the ecoregions of Upper Paraná, Ribeira do Iguape, Upper Uruguay, and South Brazilian Coast (i.e., one river showed significantly more biodiversity than any other river from the same ecoregion), but homogeneously distributed in the other ecoregions. Hence, conservation plans in the former regions will potentially require less effort than plans in the latter regions.
The objective of this research was to analyze the progression of breaker types on plane impermeable slopes. This study used dimensional analysis to demonstrate the relative water depth is a key ...explanatory quantity. The dominant breaker types depend on the incident wave characteristics at the foot of the slope. Accordingly, it is possible to combine values of H, T, and m. The physical experiments of Galvin, recent numerical results, and new experiments, performed on an impermeable 1:10 slope, were used to verify the result. It was thus possible to obtain the progression of breaker types in different sequences of pairs of combined wave H and T values. Once a sequence is defined, the expected progression of breaker types is predictable, and is well approximated by the log‐transform of the alternate similarity parameter. Since the classification of breaker types is discontinuous, the data assigned to each type were placed in horizontal lines, based on the value of log(χ). Given that the breaking of a wave train on a slope should be considered a continuous process, the location of some data was corrected to satisfy this assumption. There is thus a functional relationship between the sets of the experimental space and of the breaker types. This research also derives the non‐dimensional energy dissipation on the slope, considering the wave‐reflected energy flux on the slope. It is proportional to a dimensionless bulk dissipation coefficient which depends on the breaker type and, therefore, on the value of χ at the toe of the slope.
Plain Language Summary
The main objective of this research was to analyze the progression of breaker types on a plane impermeable slope. This study used dimensional analysis to demonstrate that the relative water depth is a key explanatory quantity. The dominant breaker types depend on the incident wave characteristics at the foot of the slope, h/L, and H/L, and also on the slope, m. Accordingly, different combinations of these three parameters can be made. Data from different data sets performed on an impermeable slope with a 1:10 slope angle were used to verify that result. After several analyses of different experimental techniques, it can be concluded that there is a functional relationship between the sets of the experimental space and the breaker types.
Key Points
The progression of breaker types depends on the slope and the wave characteristics at the toe of the slope. It is predictable
There is a functional relationship between the sets of the relative water depth and wave steepness and of breaker types
A dimensionless bulk dissipation coefficient is defined which depends on the breaker type and on the relative water depth and wave steepness
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