Ischemic stroke is the major type of cerebrovascular disease usually resulting in death or disability among the aging population globally. Oxidative stress has been closely linked with ischemic ...stroke. Disequilibrium between excessive production of reactive oxygen species (ROS) and inherent antioxidant capacity leads to subsequent oxidative damage in the pathological progression of ischemic brain injury. Acupuncture has been applied widely in treating cerebrovascular diseases from time immemorial in China. This review mainly lays stress on the evidence to illuminate the possible mechanisms of acupuncture therapy in treating ischemic stroke through regulating oxidative stress. We found that by regulating a battery of molecular signaling pathways involved in redox modulation, acupuncture not only activates the inherent antioxidant enzyme system but also inhibits the excessive generation of ROS. Acupuncture therapy possesses the potential in alleviating oxidative stress caused by cerebral ischemia, which may be linked with the neuroprotective effect of acupuncture.
Rechargeable aqueous zinc-organic batteries are promising energy storage systems with low-cost aqueous electrolyte and zinc metal anode. The electrochemical properties can be systematically adjusted ...with molecular design on organic cathode materials. Herein, we use a symmetric small molecule quinone cathode, tetraamino-p-benzoquinone (TABQ), with desirable functional groups to protonate and accomplish dominated proton insertion from weakly acidic zinc electrolyte. The hydrogen bonding network formed with carbonyl and amino groups on the TABQ molecules allows facile proton conduction through the Grotthuss-type mechanism. It guarantees activation energies below 300 meV for charge transfer and proton diffusion. The TABQ cathode delivers a high capacity of 303 mAh g
at 0.1 A g
in a zinc-organic battery. With the increase of current density to 5 A g
, 213 mAh g
capacity is still preserved with stable cycling for 1000 times. Our work proposes an effective approach towards high performance organic electrode materials.
Methodological quality (risk of bias) assessment is an important step before study initiation usage. Therefore, accurately judging study type is the first priority, and the choosing proper tool is ...also important. In this review, we introduced methodological quality assessment tools for randomized controlled trial (including individual and cluster), animal study, non-randomized interventional studies (including follow-up study, controlled before-and-after study, before-after/ pre-post study, uncontrolled longitudinal study, interrupted time series study), cohort study, case-control study, cross-sectional study (including analytical and descriptive), observational case series and case reports, comparative effectiveness research, diagnostic study, health economic evaluation, prediction study (including predictor finding study, prediction model impact study, prognostic prediction model study), qualitative study, outcome measurement instruments (including patient - reported outcome measure development, content validity, structural validity, internal consistency, cross-cultural validity/ measurement invariance, reliability, measurement error, criterion validity, hypotheses testing for construct validity, and responsiveness), systematic review and meta-analysis, and clinical practice guideline. The readers of our review can distinguish the types of medical studies and choose appropriate tools. In one word, comprehensively mastering relevant knowledge and implementing more practices are basic requirements for correctly assessing the methodological quality.
Lignocellulose is the most abundant organic carbon polymer on the earth. Its decomposition and conversion greatly impact the global carbon cycle. Furthermore, it provides feedstock for sustainable ...fuel and other value-added products. However, it continues to be underutilized, due to its highly recalcitrant and heterogeneric structure. Microorganisms, which have evolved versatile pathways to convert lignocellulose, undoubtedly are at the heart of lignocellulose conversion. Numerous studies that have reported successful metabolic engineering of individual strains to improve biological lignin valorization. Meanwhile, the bottleneck of single strain modification is becoming increasingly urgent in the conversion of complex substrates. Alternatively, increased attention has been paid to microbial consortia, as they show advantages over pure cultures, e.g., high efficiency and robustness. Here, we first review recent developments in microbial communities for lignocellulose bioconversion. Furthermore, the emerging area of synthetic ecology, which is an integration of synthetic biology, ecology, and computational biology, provides an opportunity for the bottom-up construction of microbial consortia. Then, we review different modes of microbial interaction and their molecular mechanisms, and discuss considerations of how to employ these interactions to construct synthetic consortia via synthetic ecology, as well as highlight emerging trends in engineering microbial communities for lignocellulose bioconversion.
During an outbreak of respiratory diseases including atypical pneumonia in Wuhan, a previously unknown β‐coronavirus was detected in patients. The newly discovered coronavirus is similar to some ...β‐coronaviruses found in bats but different from previously known SARS‐CoV and MERS‐CoV. High sequence identities and similarities between 2019‐nCoV and SARS‐CoV were found. In this study, we searched the homologous templates of all nonstructural and structural proteins of 2019‐nCoV. Among the nonstructural proteins, the leader protein (nsp1), the papain‐like protease (nsp3), the nsp4, the 3C‐like protease (nsp5), the nsp7, the nsp8, the nsp9, the nsp10, the RNA‐directed RNA polymerase (nsp12), the helicase (nsp13), the guanine‐N7 methyltransferase (nsp14), the uridylate‐specific endoribonuclease (nsp15), the 2'‐O‐methyltransferase (nsp16), and the ORF7a protein could be built on the basis of homology templates. Among the structural proteins, the spike protein (S‐protein), the envelope protein (E‐protein), and the nucleocapsid protein (N‐protein) can be constructed based on the crystal structures of the proteins from SARS‐CoV. It is known that PL‐Pro, 3CL‐Pro, and RdRp are important targets for design antiviral drugs against 2019‐nCoV. And S protein is a critical target candidate for inhibitor screening or vaccine design against 2019‐nCoV because coronavirus replication is initiated by the binding of S protein to cell surface receptors. It is believed that these proteins should be useful for further structure‐based virtual screening and related computer‐aided drug development and vaccine design.
Highlights
High sequence identities between 2019‐nCoV and SARS‐CoV were found. Homology templates of all structural proteins of 2019‐nCoV were identified. Homology templates of all nonstructural proteins of 2019‐nCoV were identified.
•A 2D analytical wind turbine wake model named Jensen–Gaussian wake model is proposed.•The velocity deficit predicted by Jensen–Gaussian model is validated with literature.•The Jensen–Gaussian model ...is improved by a proposed turbulence model.•The Jensen–Gaussian wake model is applied for the turbine layout optimization.•The performance of Jensen–Gaussian model on turbine layout optimization is validated.
The development and validation of a 2D analytical wind turbine wake model based on Jensen’s wake model using Gaussian function is presented in this paper. The velocity deficit predicted by the newly-developed Jensen–Gaussian wake model is compared with wind tunnel experimental measured data in literatures and results show that, the velocity deficit predicted by the model fits well with the measured data at different downwind distances of X=2.5D, X=5D, X=7.5D and X=10D. Considering the turbulence inside the turbine wake, a new turbulence model is developed and based on this, the Jensen–Gaussian wake model was improved and validated. The 2D Jensen–Gaussian wake model is then applied in the wind turbine layout optimizing process within a wind farm based on the multiple populations genetic algorithm (MPGA). The performance of this newly 2D model in the optimization process is validated and compared with the results presented in some typical studies on the turbine layout optimization. The comparison is performed for ‘constant wind speed of 12m/s with variable wind directions’. Using the 2D Jensen–Gaussian wake model instead of Jensen’s wake model in the MPGA turbine layout optimization program, both the total power generation and wind farm efficiency decreased. The wind farm efficiency drop to 77.83%, 78.47% and 81.84% from 96.83%, 96.34% and 96.23% for 38, 39 and 40 wind turbines, respectively which is in accordance with the literatures on the power losses caused by wake effect in large wind farm. The development and application of the 2D Jensen–Gaussian wake model means moretheorysignificance and practicalvalues in wind energy utilization.
Objective
To assess the efficacy of intensive acupuncture (3 times weekly for 8 weeks) versus sham acupuncture for knee osteoarthritis (OA).
Methods
In this multicenter, randomized, sham‐controlled ...trial, patients with knee OA were randomly assigned to receive electroacupuncture (EA), manual acupuncture (MA), or sham acupuncture (SA) 3 times weekly for 8 weeks. Participants, outcome assessors, and statisticians were blinded with regard to treatment group assignment. The primary outcome measure was response rate, which is the proportion of participants who simultaneously achieved minimal clinically important improvement in pain and function by week 8. The primary analysis was conducted using a Z test for proportions in the modified intent‐to‐treat population, which included all randomized participants who had ≥1 post‐baseline measurement.
Results
Of the 480 participants recruited in the trial, 442 were evaluated for efficacy. The response rates at week 8 were 60.3% (91 of 151), 58.6% (85 of 145), and 47.3% (69 of 146) in the EA, MA, and SA groups, respectively. The between‐group differences were 13.0% (97.5% confidence interval 97.5% CI 0.2%, 25.9%; P = 0.0234) for EA versus SA and 11.3% (97.5% CI −1.6%, 24.4%; P = 0.0507) for MA versus SA. The response rates in the EA and MA groups were both significantly higher than those in the SA group at weeks 16 and 26.
Conclusion
Among patients with knee OA, intensive EA resulted in less pain and better function at week 8, compared with SA, and these effects persisted though week 26. Intensive MA had no benefit for knee OA at week 8, although it showed benefits during follow‐up.
Variants of TREM2 are associated with Alzheimer’s disease (AD). To study whether increasing TREM2 gene dosage could modify the disease pathogenesis, we developed BAC transgenic mice expressing human ...TREM2 (BAC-TREM2) in microglia. We found that elevated TREM2 expression reduced amyloid burden in the 5xFAD mouse model. Transcriptomic profiling demonstrated that increasing TREM2 levels conferred a rescuing effect, which includes dampening the expression of multiple disease-associated microglial genes and augmenting downregulated neuronal genes. Interestingly, 5xFAD/BAC-TREM2 mice showed further upregulation of several reactive microglial genes linked to phagocytosis and negative regulation of immune cell activation. Moreover, these mice showed enhanced process ramification and phagocytic marker expression in plaque-associated microglia and reduced neuritic dystrophy. Finally, elevated TREM2 gene dosage led to improved memory performance in AD models. In summary, our study shows that a genomic transgene-driven increase in TREM2 expression reprograms microglia responsivity and ameliorates neuropathological and behavioral deficits in AD mouse models.
•Elevating TREM2 gene dosage altered microglial morphology and interaction with Aβ•Increasing TREM2 gene dosage reprograms microglial responsivity in AD mouse brains•Transcriptomic profiling identified three groups of TREM2 gene-dosage-dependent genes•Extra TREM2 gene dosage ameliorates neuropathology and memory deficits in AD mice
Augmenting TREM2 gene dosage in AD mouse models leads to reduced amyloid burden and neuropathology and improved memory performance. Gene expression profiling reveals a reprogrammed disease-associated microglial response that may underlie the phenotypic improvement in AD models.
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•Material intrinsic property effects on the electrocapacitive performance are studied.•Core/shell morphology variation is minimized and restricted to a sheet-on-sheet one.•NiCo2O4 ...core materials with the NiSx, NiOx, NiMoxOy, and MnOx shells are synthesized.•The NiCo2O4/NiSx electrode got a specific capacitance of 6.12Fcm−2 at 1mAcm−2.•An asymmetric supercapacitor with the NiCo2O4/NiSx and carbon electrodes is made.
Core/shell nanostructures of metal oxides and sulfides are intensively studied as the energy-storage materials. Most researchers focus on a single core/shell system and optimize the morphology of nanomaterials to enhance the electrochemical performance, but limited reports studying the effect of the material category in the same morphology on the performance of the core/shell electrode. In this work, multiple core/shell systems based on the similar sheets-on-sheets morphology are made using the same NiCo2O4 core and different shells of NiSx, NiOx, NiMoxOy and MnOx. By minimizing the effects of the morphology for the core/shell structure, the material category is verified to play important roles on the electrochemical performances of the electrodes. The NiCo2O4/NiSx, NiCo2O4/NiOx, and NiCo2O4/NiMoxOy core/shell electrodes present larger specific capacitance (CF) but an unexpected smaller CF value is obtained for the NiCo2O4/MnOx core/shell electrode as compared with that for the single NiCo2O4 nanosheet electrode, suggesting the depositing of the shell to enhance the surface area for conducting more Faradic reactions is not always helpful on improving the electrocapacitive performance of the electrode. The highest CF value of 6.12Fcm−2 is obtained for the NiCo2O4/NiSx electrode evaluated by using the galvanic charge/discharge curves at the current density of 1mAcm−2, due to the superior intrinsic property of NiSx other than its high surface area of the sheets-on-sheets morphology since the structure variation is almost excluded in the work. The asymmetric supercapacitor (ASC) based on the NiCo2O4/NiSx positive electrode is also made with the CF value of 28.6Fg−1 based on the whole mass of the ASC at the current density of 0.5Ag−1 as well as the maximum energy density of12.83Whkg−1 at a power density of 80Wkg−1.
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