The present experiment was conducted to determine the methionine requirement and the effect of dietary methionine levels on growth, feed utilization, body composition, haematological and ...morphometrical parameters in juvenile grouper. Diet 1 with fish meal as the sole protein source was used as a reference. Diets 2–7 were formulated to contain 48% crude protein and supplemented with graded concentrations of
l-methionine, resulting in six levels of dietary methionine (0.55–1.81% of dry matter) at a constant dietary cystine level of 0.26%. Fish (initial weight of 13.25
±
0.19 g, mean
±
S.D.) were reared in twenty-one 500-l circular fibreglass tanks provided with flow-through seawater at 28–30.5 °C and salinity of 24–28 mg/l for 8 weeks. Fish fed the reference diet had significantly higher weight gain (WG), specific growth rate (SGR), feed utilization efficiency, protein and lipid contents of whole body, plasma protein and cholesterol concentrations (
P
<
0.05). Among other treatments fed the diets containing CAAs, increasing dietary methionine up to 1.34% increased growth performance and feed utilization, whole-body protein and lipid contents, condition factor (CF), viscerosomatic index (VSI) and intraperitoneal fat ratio (IPR), beyond which they remained nearly unchanged. Plasma protein concentration showed no significant differences for fish fed the diet containing more than 1.11% methionine. Triacylglycerol increased with increasing dietary methionine levels, but showed a relatively lower value for fish fed the 1.59% methionine diet. Plasma glucose could not be related to dietary treatments. Cholesterol was the highest for fish fed the 1.34% methionine diet but showed no significant difference among other treatments. Fish fed higher-methionine diets exhibited relatively higher essential amino acid (EAA) contents in muscle than fish fed the lower methionine diet (
P
<
0.05). Broken-line regression analysis of WG against dietary methionine level indicated that optimal dietary methionine requirement for juvenile grouper was 1.31% of the diet (corresponding to 2.73% of dietary protein on a dry weight basis).
Although the discrete Fourier transform (DFT) based pulse shape discrimination (PSD) method, realized by transforming the digitized scintillation pulses into frequency coefficients by using DFT, has ...been proven to effectively discriminate neutrons and γ rays, its discrimination performance depends strongly on the selection of the discrimination parameter obtained by the combination of these frequency coefficients. In order to thoroughly understand and apply the DFT-based PSD in organic scintillation detectors, a comparison of three different discrimination parameters, i.e. the amplitude of zero-frequency component, the amplitude difference between the amplitude of zero-frequency component and the amplitude of base-frequency component, and the ratio of the amplitude of base-frequency component to the amplitude of zero-frequency component, is described in this paper. An experimental setup consisting of an Americium–Beryllium (Am–Be) source, a BC501A liquid scintillator detector, and a 5Gsample/s 8-bit oscilloscope was built to assess the performance of the DFT-based PSD with each of these discrimination parameters in terms of the figure-of-merit (based on the separation of the event distributions). The third technique, which uses the ratio of the amplitude of base-frequency component to the amplitude of zero-frequency component as the discrimination parameter, is observed to provide the best discrimination performance in this research.
•The spectrum difference between neutron pulse and γ-ray pulse was investigated.•The DFT-based PSD with different parameter definitions was assessed.•The way of using the ratio of magnitude spectrum provides the best performance.•The performance differences were explained from noise suppression features.
Using 2.93 fb(-1) of e(+)e(-) collision data taken with the BESIII detector at a center-of-mass energy of 3.773 GeV, the observation of the D-0 -> K-1 (1270)(-)e(+)nu(e) semileptonic decay is ...presented. The statistical significance of the decay D-0 -> K-1 (1270)e(+)nu(e) is greater than 10 sigma. The branching fraction of D-0 -> K-1 (1270)(-)e(+)nu(e) is measured to be (1.09 +/- 0.13(-0.16)(+0.09) +/- 0.12) x 10(-3). Here, the first uncertainty is statistical, the second is systematic, and the third originates from the assumed branching fraction of K-1 (1270)(-) -> K-pi(+)pi(-). The fraction of longitudinal polarization in D-0 -> K-1 (1270)(-)e(+)nu(e) is determined for the first time to be 0.50 +/- 0.19(stat )+/- 0.08(syst).
Using the data samples collected in the energy range from 3.773 to 4.600 GeV with the BESIII detector at the BEPCII collider, we measure the dressed cross sections as a function of center-of-mass ...energy for e(+)e(-) -> K+K- pi(+)pi(-) (pi(0)), K+K-K+K- (pi(0)), pi(+)pi(-) pi(+)pi(-) (pi(0)), and p (p) over bar pi(+)pi(-)(pi(0)). The cross sections for e(+)e(-) -> K+K-K+K- pi(0), p (p) over bar pi(+)pi(-)(pi(0)) are the first measurements. Cross sections for the other five channels are much more precise than previous results in this energy region. We also search for charmonium and charmonium-like resonances, such as the Y(4230), decaying into the same final states. We find evidence of the psi(4040) decaying to pi(+)pi(-) pi(+)pi(-) pi(0) with a statistical significance of 3.6 sigma. Upper limits are provided for other decays since no clear signals are observed.
We report new measurements of the branching fraction B(Dv), where lis either mu or tau (-> pi), based on 6.32 fbof electron-positron annihilation data collected by the BESIII experiment at six ...center-of-mass energy points between 4.178 and 4.226 GeV. Simultaneously floating the Ds -> mu mu components yields B(D -> tau) = (5.21 +/- 0.25 +/- 0.17) x 102, B(D -> mu mu) (5.35 +/- 0.13 +/- 0.16) x 103, and the ratio of decay widths R = Gamma(D -> tau tau)/Gamma -> mu&m)= 9.73-0.58 +/- 0.36, where the first uncertainties are statistical and the second systematic. No evidence of CP asymmetry is observed in the decay rates Ds +/- -> mu +/- v mu and D +/- -> tau +/-= (+2.9 +/- 4.8 +/- 1.0)%. Constraining our measurement to the Standard Model expectation of lepton universality (R = 9.75), we find the more precise results B(D -> tau = (5.22 +/- 0.10 +/- 0.14) x 10-2and A(+/-) = (-0.1 +/- 1.9 +/- 1.0)%. Combining our results with inputs external to our analysis, we determine the C -> (S) over bar quark mixing matrix element, D+ decay constant, and ratio of the decay constants to be vertical bar V vertical bar = 0.973 +/- 0.009 +/- 0.014, f D+ = 249.9 +/- 2.4 +/- 3.5 MeV, and f D+ / f D = 1.232 +/- 0.035, respectively.
To study the clinical and pathological characteristics of HPV-negative cervical cancer patients.
Retrospective analysis of 785 cervical cancer patients in Guangdong Women and Children Hospital from ...Jan. 2005 to Oct. 2015. By detecting high- risk HPV infection by flow-through hybridization genechip technique.
(1)Among 785 cases of cervical cancer, 71 cases were negative for HPV infection tested by genechip technique, accounting for 9.0%(71/785), and the relative light units/cut off(RLU/CO)ratios of these 71 cases were less than 1 by hybird capture Ⅱ(HC-Ⅱ)methods. The results showed that the positive coincident rate of genechip technique detecting result with HC-Ⅱ method was 100.0%(71/71).(2)There was no difference between 43(60.6%)cases from 41-55 years old of 71 cases of HPV-negative patients and 392(54.9%)cases from 41- 55 years old of 714 cases of HPV infection patients(
=15.63,
=0.571). Among 71 cases of HPV-negative patients, 32 cases of patients with doing TCT, 6(18.8%)cases for normal, 10(31.2%)cases fo
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