Bovine mastitis is a costly disease in the dairy sector worldwide. Here the objective was to identify and characterize anti-biofilm compounds produced by Bacillus spp. against S. aureus associated ...with bovine mastitis. Results showed that cell-free supernatants of three Bacillus strains (out of 33 analysed) reduced S. aureus biofilm formation by approximately 40 % without affecting bacterial growth. The anti-biofilm activity was associated with exopolysaccharides (EPS) secreted by Bacillus spp. The EPS decreased S. aureus biofilm formation in a dose-dependent manner, inhibiting biofilm formation by 83 % at 1 mg/mL. The EPS also showed some biofilm disruption activity (up to 36.4 %), which may be partially mediated by increased expression of the aur gene. The characterization of EPS produced by Bacillus velezensis 87 and B. velezensis TR47II revealed macromolecules with molecular weights of 31.2 and 33.7 kDa, respectively. These macromolecules were composed mainly of glucose (mean = 218.5 μg/mL) and mannose (mean = 241.5 μg/mL) and had similar functional groups (pyranose ring, beta-type glycosidic linkage, and alkynes) as revealed by FT-IR. In conclusion, this study shows the potential applications of EPS produced by B. velezensis as an anti-biofilm compound that could contribute to the treatment of bovine mastitis caused by S. aureus.
•Exopolysaccharides synthesized by Bacillus spp. inhibit biofilm formation by S. aureus associated with bovine mastitis.•The inhibition of the biofilm formation is dose-dependent and highest (83 %) at 1000 μg/mL.•Exopolysaccharides have a small effect in stimulating biofilm disruption.•The EPS is mainly composed of glucose and mannose and has a molecular weight of approximately 30 kDa.•This is the first report describing the anti-biofilm activity of EPS against bovine mastitis pathogens.
Grinding wheel content characterization is still mainly based on empirical tests, lacking the necessary scientific approach. To further develop the current existing methodology, this study introduces ...a method to characterize the grinding wheel structure and content, using microscale X-ray tomography allied with image processing techniques. An X-ray tomography characterization was performed on four different conventional grinding wheels, each containing a specific mixture of electro-fused and sol-gel aluminum oxide abrasives as grits. The grinding wheels were evaluated in terms of volumetric phase fraction, representative elementary volume, phase, and pore size distribution. The results showcased that even though the grinding wheels presented the same manufacturing label (in terms of mechanical properties), the volumetric phase fraction between the grinding wheels was significantly different. This study aggregates the empirical knowledge used to characterize grinding wheels, opening possibilities for academic and industrial research.
Graphical abstract
Fetal Alcohol Spectrum Disorders: Health Needs Assessment in Brazil Rocha, Anastácia Guimarães; Souza, Paulo Ricardo Assis; Wachholz, Gabriela Elis ...
Alcoholism, clinical and experimental research,
March 2020, 2020-Mar, 2020-03-00, 20200301, Letnik:
44, Številka:
3
Journal Article
Recenzirano
Background
Fetal alcohol syndrome (FAS) is a disorder caused by alterations in embryo‐fetal development due to prenatal alcohol exposure. It is estimated that between 0.5 and 2 per 1,000 individuals ...are born with FAS every year. In Brazil, there are few studies addressing the extent of the problem of FAS/fetal alcohol spectrum disorders (FASD); these studies are confined to limited geographic areas. Therefore, we decided to perform a health needs assessment for FAS/FASD in Brazil.
Methods
To estimate the prevalence of FAS and FASD in Brazil, we used information from the literature, which estimates between 0.5 and 2/1,000 births per year for FAS and 10 to 50/1,000 for FASD.
Results
We estimated that approximately 1,500 to 6,000 children are born with FAS every year. Considering the whole population, the prevalence would be 95,377 to 380,000 affected people. However, when we consider FASD as a whole, we estimate that between 1,900,000 and 9,500,000 Brazilians might suffer the more severe consequences of alcohol exposure during pregnancy and be living with FASD.
Conclusion
The results of the current study indicate that FAS and FASD are prevalent disorders in Brazil, and more policies targeting alcohol intake during pregnancy must be developed.
SARS-CoV-2 virus was first identified in the beginning of 2020 and has spread all over the world, causing the Coronavirus Disease 2019 (COVID-19) pandemic. The virus is a member of the Coronavirus ...family, which includes viruses that cause common cold, Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). MERS and SARS are known by causing adverse events in pregnancy. Considering that SARS-CoV-2 is a new infection agent, little is known about the risk of its infection to human embryo/fetal development. However, SARS and MERS were associated with negative outcomes, such as miscarriage, preterm birth, intrauterine growth restriction and perinatal death. Here, we raise concerns and possibilities related the harmful potential of SARS-CoV-2 and COVID-19 to pregnancy, discussing symptoms, immunological changes during pregnancy, SARS-CoV-2 mutation rate (and the risks related to it). Finally, we point out recommendations to be performed by the scientific community and health care workers in order to identify and to manage potential risks to pregnant women and their babies.
Caffeine intake during pregnancy is common. Caffeine crosses the placenta, raising concerns about its possible deleterious effects on the developing embryo/fetus. Studies on this subject show ...conflicting results, and still there is no consensus on the recommended dose of caffeine during pregnancy. We performed an integrative review with studies from six databases, using broad MESH terms to allow the identification of publications that addressed the outcomes of caffeine use during pregnancy, with no date limit for publications, in English and Portuguese language. The research returned 16,192 articles. After removing duplicates, screening by title, abstract and full-text, we evaluated 257 and included 59 articles. We found association between caffeine intake and pregnancy loss, low birth weight, cardiac and genital anomalies, higher body mass, and neurodevelopmental and neurobehavioral outcomes. The effects were often dose dependent. No association with prematurity has been demonstrated, but one study showed a small reduction in gestational age with increasing doses of caffeine intake. Defining a safe dose for caffeine intake during pregnancy is a challenging task due to the heterogeneity in study designs and results, as well as the difficulty of reliably assessing the amount of caffeine consumed. In some studies, exposures below the recommended level of caffeine intake during pregnancy (200 mg/day), as suggested by the guidelines, were associated with pregnancy loss, low birth weight, cardiac and genital anomalies, higher body mass, and neurodevelopmental and neurobehavioral outcomes. Well-designed studies with reliable quantification of caffeine intake are needed to assess the safety of low doses during pregnancy.
The present study tested the effects of a newly identified indolin-3-one compound (compound 1), produced by Pseudomonas aeruginosa, on HepG2 cells. The MTT assays demonstrated decreased metabolic ...activities in HepG2 cells treated with compound 1, with dose- and time-dependent intensifying effect, starting at a concentration of 40 µM. The IC
after 24, 48, 72, and 96 h treatments were 41.35, 52.7, 92.79 and 66.65 μM of compound 1, respectively. Below 80 µM, no significative damage on erythrocytes membranes was observed by the hemolytic assays. The RT-qPCR revealed that the compound modulated key genes involved in carcinogenesis process, indicating possible indolin-3-one mechanisms of action. The data showed that gene expression alterations promoted by compound 1, in concentrations up to 60 μM after 48 h, led to a decrease in cellular progression and there was no direct cellular damage. In addition, non-cytotoxic concentrations of compound 1 halved the concentration of the chemotherapeutic doxorubicin, maintaining similar therapeutic effect against HepG2 cells. The novelty of the molecule and the biological activities observed in the present study emphasize the potential of the compound 1 in cancer therapy research.
ABSTRACT
Background:
Cognitive decline is a common condition, but is still underrepresented in studies conducted in developing countries.
Objective:
To calculate the prevalence of cognitive decline ...and depression in an elderly community-dwelling population in a city in southern Brazil.
Methods:
We calculated the prevalences of dementia, cognitive impairment with no dementia (CIND) and symptoms of depression in an elderly population relying on the public healthcare system. This epidemiological study in Pelotas, Brazil, was conducted within the primary care setting. It included 299 older adults (mean age = 69.75 ± 7.6 years) who presented low levels of education (mean = 4.16 ± 3.17 years of education). They underwent cognitive screening and their medical records were analyzed.
Results:
Among these older adults, 142 (47.5%) presented cognitive decline: 104 (34.8%) matching the cognitive criteria for CIND and 38 (12.7%) matching the cognitive criteria for dementia. Among all the individuals who completed the cognitive screening, 141 (48.4%) were positive for symptoms of depression, of whom 99 (34%) did not have any previous diagnosis in their medical records.
Conclusion:
There was high prevalence of cognitive impairment among these older adults in a primary care setting. A large number of older adults were found to have symptoms of depression without any diagnosis.
The discovery of a new boson with a mass of approximately 125 GeV in 2012 at the Large Hadron Collider1, 2, 3 has heralded a new era in understanding the nature of electroweak symmetry breaking and ...possibly completing the standard model of particle physics4, 5, 6, 7, 8, 9. Since the first observation in decays to γγ, WW and ZZ boson pairs, an extensive set of measurements of the mass10, 11 and couplings to W and Z bosons11, 12, 13, as well as multiple tests of the spin-parity quantum numbers10, 11, 13, 14, have revealed that the properties of the new boson are consistent with those of the long-sought agent responsible for electroweak symmetry breaking. An important open question is whether the new particle also couples to fermions, and in particular to down-type fermions, as the current measurements mainly constrain the couplings to the up-type top quark. Determination of the couplings to down-type fermions requires direct measurement of the corresponding Higgs boson decays, as recently reported by the Compact Muon Solenoid (CMS) experiment in the study of Higgs decays to bottom quarks15 and τ leptons16. Here, we report the combination of these two channels, which results in strong evidence for the direct coupling of the 125 GeV Higgs boson to down-type fermions, with an observed significance of 3.8 standard deviations, when 4.4 are expected.
This paper proposes a new optimization methodology to solve the AC optimal power flow (OPF) problem considering renewable energy sources (RES). The formulation of the OPF problem comprises the ...minimization of power generation costs and gas emissions considering a set of operational and physical constraints. This minimization is achieved through controlling power dispatch generators, position changing of the tap transformers, and controllable reactive shunt compensation. RES and demand uncertainties are modeled using the (2m+1) point-estimate method. The mathematical formulation of the OPF problem is a mixed-integer nonlinear programming multiobjective model. A matheuristic algorithm is proposed to solve this problem efficiently, combining a classic nonlinear OPF model and the Variable Neighborhood Descent (VND) metaheuristic algorithm. The potential of the proposed algorithm is shown through numerical experiments carried out using the IEEE 300-bus systems.
In the present study two extreme events that occurred in the East Coast of Northeast Brazil (ENEB) during 2022 and 2023 were evaluated. These events are becoming increasingly frequent in all regions ...of Brazil, associated with significant material and human losses, emphasizing the significance of a deeper comprehension of these events. ERA5 global reanalysis data, GOES-16 satellite imagery and pluviometric stations were used for the analysis. Model simulations were also conducted using the Model for Prediction Across Scales (MPAS) with variable resolution (60–3 km). Both events corresponded to Easterly Wave Disturbances (EWDs) that occurred under opposite large-scale conditions of the ENSO cycle, since extreme events are becoming increasingly frequent in all regions of Brazil and could be responsible for significant material and human losses. Thus, an emphasis was given to characterize the synoptic conditions. Both analyzed cases occurred along the ENEB, specifically over the Alagoas state. The trough axis penetrating the studied area was observed on both examined dates, with a very characteristic relative vorticity of this tropical disturbance. In general, moisture convergence resulted from the high flow of moisture prevailing over the region combined with upward movements caused by the trough present at low levels, which combined with local factors in the region such as topography, contributed to the increase in rainfall over the study area in both analyzed cases. The MPAS showed excellent spatial representation when compared to station data, highlighting intense precipitation over parts of Alagoas.
•The study highlights the impacts of the Easterly Waves under different configurations of ocean-atmosphere conditions.•Local factors such as topography could contribute to the increase in rainfall values in the study area.•This is the first study to use the MPAS model to simulate extreme precipitation events over the NEB.•MPAS simulations was able to simulate both the spatial distribution and the intensity of the observed precipitation.
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