Migrasomes are newly discovered extracellular vesicles (EVs) that are formed in migrating cells and mediate intercellular communication. However, their size, biological generation, cargo packaging, ...transport, and effects on recipient cells by migrasomes are different from those of other EVs. In addition to mediating organ morphogenesis during zebrafish gastrulation, discarding damaged mitochondria, and lateral transport of mRNA and proteins, growing evidence has demonstrated that migrasomes mediate a variety of pathological processes. In this review, we summarize the discovery, mechanisms of formation, isolation, identification, and mediation of cellular communication in migrasomes. We discuss migrasome-mediated disease processes, such as osteoclast differentiation, proliferative vitreoretinopathy, tumor cell metastasis by PD-L1 transport, immune cell chemotaxis to the site of infection by chemokines, angiogenesis promotion via angiogenic factors by immune cells, and leukemic cells chemotaxis to the site of mesenchymal stromal cells. Moreover, as new EVs, we propose the potential of migrasomes for disease diagnosis and treatment. Video Abstract.
Exosomes are critically involved in cancer development and progression. The exosomal contents have been suggested as ideal cancer biomarkers. In this study, we investigated the expression of exosomal ...proteins in the serum of gastric cancer patients and their roles in gastric cancer.
The proteomic profile of exosomes from the serum of gastric cancer patients was detected by using LC-MS/MS. The expression of TRIM3 in exosomes from the serum of gastric cancer patients and healthy controls was assessed by ELISA and western blot. Immunohistochemistry was used to detect TRIM3 expression in gastric cancer tissues and their matching adjacent tissues. The growth and migration abilities of gastric cancer cells with TRIM3 overexpression or knockdown in vitro were evaluated by colony formation assay and transwell migration assay. The effects of TRIM3 overexpression or knockdown on gastric cancer growth and metastasis in vivo were investigated by using subcutaneous xenograft tumor and peritoneal metastasis mouse model. The effects of TRIM3-overexpressing exosomes on gastric cancer growth and metastasis in vitro and in vivo were also evaluated.
We found that the expression levels of TRIM3 mRNA and protein were decreased in gastric cancer tissues compared to the matched control tissues. In addition, the levels of TRIM3 protein in the serum exosomes of gastric cancer patients were lower than that in healthy controls. We demonstrated that TRIM3 overexpression reduced while TRIM3 knockdown promoted the growth and metastasis of gastric cancer in vitro and in vivo through the regulation of stem cell factors and EMT regulators. Moreover, exosomes-mediated delivery of TRIM3 protein could suppress gastric cancer growth and metastasis in vitro and in vivo.
Taken together, our findings suggest that exosomal TRIM3 may serve as a biomarker for gastric cancer diagnosis and the delivery of TRIM3 by exosomes may provide a new avenue for gastric cancer therapy.
Mutations in optineurin (OPTN) are associated with several human disorders including amyotrophic lateral sclerosis (ALS) and primary open-angle glaucoma (POAG). OPTN is known to be a multifunctional ...autophagy receptor that plays important roles in NF-κB signaling, vesicle trafficking, maintenance of the Golgi apparatus and autophagy. Given that a loss of neurons and an abnormal aggregation of disease proteins are two key features of neurodegenerative diseases, protein quality control systems are considered to be tightly associated with neurodegeneration. In this study, we investigated the involvement of the ubiquitin-proteasome system (UPS) and the autophagy-lysosome pathway, two major intracellular protein quality control systems, in the regulation of wild-type (WT) OPTN, ALS-linked mutant E478G OPTN and POAG-linked mutant E50K OPTN. Our data revealed that the UPS, not the autophagy-lysosome pathway, is the major system for degradation and aggregation of OPTN. Moreover, we found that Hrd1, an E3 ubiquitin ligase, could play an important role in the protein quality control of OPTN. Our results demonstrated that overexpression of Hrd1 increased the proteasomal degradation and microtubule-dependent aggresome formation of OPTN in the microtubular organizing center, whereas knockdown of Hrd1 stabilized OPTN and inhibited aggresome formation of OPTN.
Wi-Fi-based person identification (PI) tasks are performed by analyzing the fluctuating characteristics of the Channel State Information (CSI) data to determine whether the person's identity is ...legitimate. This technology can be used for intrusion detection and keyless access to restricted areas. However, the related research rarely considers the restricted computing resources and the complexity of real-world environments, resulting in lacking practicality in some scenarios, such as intrusion detection tasks in remote substations without public network coverage. In this paper, we propose a novel neural network model named SimpleViTFi, a lightweight classification model based on Vision Transformer (ViT), which adds a downsampling mechanism, a distinctive patch embedding method and learnable positional embedding to the cropped ViT architecture. We employ the latest IEEE 802.11ac 80MHz CSI dataset provided by 1. The CSI matrix is abstracted into a special “image” after pre-processing and fed into the trained SimpleViTFi for classification. The experimental results demonstrate that the proposed SimpleViTFi has lower computational resource overhead and better accuracy than traditional classification models, reflecting the robustness on LOS or NLOS CSI data generated by different Tx-Rx devices and acquired by different monitors.
Continuous-variable measure-device-independent quantum key distribution (CV-MDI QKD) is proposed to remove all imperfections originating from detection. However, there are still some inevitable ...imperfections in a practical CV-MDI QKD system. For example, there is a fluctuating channel transmittance in the complex communication environments. Here we investigate the security of the system under the effects of the fluctuating channel transmittance, where the transmittance is regarded as a fixed value related to communication distance in theory. We first discuss the parameter estimation in fluctuating channel transmittance based on these establishing of channel models, which has an obvious deviation compared with the estimated parameters in the ideal case. Then, we show the evaluated results when the channel transmittance respectively obeys the two-point distribution and the uniform distribution. In particular, the two distributions can be easily realized under the manipulation of eavesdroppers. Finally, we analyze the secret key rate of the system when the channel transmittance obeys the above distributions. The simulation analysis indicates that a slight fluctuation of the channel transmittance may seriously reduce the performance of the system, especially in the extreme asymmetric case. Furthermore, the communication between Alice, Bob and Charlie may be immediately interrupted. Therefore, eavesdroppers can manipulate the channel transmittance to complete a denial-of-service attack in a practical CV-MDI QKD system. To resist this attack, the Gaussian post-selection method can be exploited to calibrate the parameter estimation to reduce the deterioration of performance of the system.
Detecting changes in surface air temperature in mid- and low-altitude mountainous regions is essential for a comprehensive understanding of warming trend with altitude. We use daily surface air ...temperature data from 64 meteorological stations in Wuyi Mountains and its adjacent regions to analyze the spatio-temporal patterns of temperature change. The results show that Wuyi Mountains have experienced significant warming from 1961 to 2018. The warming trend of the mean temperature is 0.20°C/decade, the maximum temperature is 0.17°C/decade, and the minimum temperature is 0.26°C/decade. In 1961–1990, more than 63% of the stations showed a decreasing trend in annual mean temperature, mainly because the maximum temperature decreased during this period. However, in 1971–2000, 1981–2010 and 1991–2018, the maximum, minimum and mean temperatures increased. The fastest increasing trend of mean temperature occurred in the southeastern coastal plains, the quickest increasing trend of maximum temperature occurred in the northwestern mountainous region, and the increase of minimum temperature occurred faster in the southeastern coastal and northwestern mountainous regions than that in the central area. Meanwhile, this study suggests that elevation does not affect warming in the Wuyi Mountains. These results are beneficial for understanding climate change in humid subtropical middle and low mountains.
miRNAs play a crucial part in multiple biological processes of cell proliferation, migration, apoptosis, and chemoresistance. In cancer, miRNAs can be divided into oncogenes or tumor suppressors on ...the basis of their functions in the carcinogenic process. The purpose of this study was to explore the roles and clinical diagnostic value of miR-370-3p in breast cancer. Our results demonstrated that miR-370-3p significantly promoted proliferation, metastasis, and stemness of breast cancer in vitro and in vivo. In particular, clinical data revealed that high expression of serum miR-370-3p and exosomal miR-370-3p from breast cancer patients was remarkably correlated with lymphatic metastasis and tumor node metastasis (TNM) stages. Mechanistically, miR-370-3p inhibited FBLN5 expression and activated the NF-κB signaling pathway to promote breast cancer cell proliferation, migration, and stemness. FBLN5 expression was significantly decreased in breast cancer cells and tumor tissues of breast cancer patients. Our research identified that miR-370-3p promoted breast cancer progression by inhibiting FBLN5 expression and activating the NF-κB signaling pathway. Serum exosomal miR-370-3p would provide a potential biomarker for the diagnosis of breast cancer.
In quantum key distribution (QKD), there are some security loopholes opened by the gaps between the theoretical model and the practical system, and they may be exploited by eavesdroppers (Eve) to ...obtain secret key information without being detected. This is an effective quantum hacking strategy that seriously threatens the security of practical QKD systems. In this paper, we propose a new quantum hacking attack on an integrated silicon photonic continuous-variable quantum key distribution (CVQKD) system, which is known as a power analysis attack. This attack can be implemented by analyzing the power originating from the integrated electrical control circuit in state preparation with the help of machine learning, where the state preparation is assumed to be perfect in initial security proofs. Specifically, we describe a possible power model and show a complete attack based on a support vector regression (SVR) algorithm. The simulation results show that the secret key information decreases with the increase of the accuracy of the attack, especially in a situation with less excess noise. In particular, Eve does not have to intrude into the transmitter chip (Alice), and may perform a similar attack in practical chip-based discrete-variable quantum key distribution (DVQKD) systems. To resist this attack, the electrical control circuit should be improved to randomize the corresponding power. In addition, the power can be reduced by utilizing the dynamic voltage and frequency scaling (DVFS) technology.
Primary cardiac tumors are extremely uncommon and primary cardiac lymphoma (PCL) is an even rarer subset. A definite diagnosis can be delayed, which increases the likelihood of a poor prognosis. We ...report a case involving a 64-year-old male who presented with dyspnea, palpitation, and third-degree atrioventricular block (AVB) secondary to primary cardiac B-cell lymphoma that was diagnosed
endomyocardial biopsy (EMB) and multimodality imaging. Chemotherapy was initiated using rituximab, cyclophosphamide, vindesine, and prednisone (R-COP) followed by implantation of an artificial capsule pacemaker. Third-degree AVB vanished, and the subsequent cycle of treatment was adjusted as R-CDOP (rituximab, cyclophosphamide, doxorubicin liposome, vindesine, and prednisone), with aspirin and rosavastatin to prevent ischemic events. So far, the patient had a good clinical course and normal electrocardiogram. This case underscores the importance of EMB in the diagnosis of heart neoplasms. It is worth noting that anthracycline is not contraindicated in PCL.
Background
Heat shock response is a protected mechanism against environmental changes for the organism, which must be tightly regulated. Bromodomain and extra terminal-containing protein family ...(BETs) regulate numerous gene expression in many physiological and pathological conditions, including viral infection. SV40 is considered as a highly human disease-associated virus.
Objective
We aimed to explore whether BETs play a role in heat shock in SV40 large T antigen transfected cells.
Methods
SV40LTA was transfected in HeLa cells using the Lipofectamine 8000. BETs inhibitor JQ1 and I-BET-762 was employed to treat transfected cells and HEK-293 T cells. Heat shock treatment was performed to determine the effect of JQ1 and I-BET-762 on these cells. Western blot and quantitative RT-PCR were carried out to assess the expression of HSP70 and other HSPs.
Results
We found that inhibition of BETs by JQ1 and I-BET-762 protects cells from heat shock-induced death in HEK293T cells. Both JQ1 and I-BET-762 induce the expression of HSPs and HSF1 in HEK-293 T cells. However, neither JQ1 nor I-BET-762 fail to induce the expression of HSPs in either HeLa or HBL-1 cells. When SV40 large T antigen was transfected into HeLa cells, the induction of HSP70 expressing and the protection of heat shock-induced cell death are reproduced by JQ1 and IBET treatment in these transfected cells.
Conclusions
Inhibition of BETs by JQ1 and I-BET-762 prevents heat shock-induced cell death via upregulating HSPs in SV40 large T antigen transfected cells. Our data indicate a novel function of BETs in SV40 large T antigen transformed cells, affecting HSPs and HSF1 as well as its function on heat shock response.
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