Insects are the largest group of animals. They are capable of surviving in virtually all environments from arid deserts to the freezing permafrost of polar regions. This success is due to their great ...capacity to tolerate a range of environmental stresses, such as low temperature. Cold/freezing stress affects many physiological processes in insects, causing changes in main metabolic pathways, cellular dehydration, loss of neuromuscular function, and imbalance in water and ion homeostasis. The neuroendocrine system and its related signaling mediators, such as neuropeptides and biogenic amines, play central roles in the regulation of the various physiological and behavioral processes of insects and hence can also potentially impact thermal tolerance. In response to cold stress, various chemical signals are released either via direct intercellular contact or systemically. These are signals which regulate osmoregulation - capability peptides (CAPA), inotocin (ITC)-like peptides, ion transport peptide (ITP), diuretic hormones and calcitonin (CAL), substances related to the general response to various stress factors - tachykinin-related peptides (TRPs) or peptides responsible for the mobilization of body reserves. All these processes are potentially important in cold tolerance mechanisms. This review summarizes the current knowledge on the involvement of the neuroendocrine system in the cold stress response and the possible contributions of various signaling molecules in this process.
Drosera intermedia grows in acidic bogs in parts of valleys that are flooded in winter, and that often dry out in summer. It is also described as the sundew of the most heavily hydrated habitats in ...peatlands, and it is often found in water and even underwater. This sundew is the only one that can tolerate long periods of submersion, and more importantly produces a typical submerged form that can live in such conditions for many years. Submerged habitats are occupied by D. intermedia relatively frequently. The aim of the study was to determine the environmental conditions and architecture of individuals in the submerged form of D. intermedia. The features of the morphological and anatomical structure and chlorophyll a fluorescence of this form that were measured were compared with analogous ones in individuals that occurred in emerged and peatland habitats. The submerged form occurred to a depth of 20 cm. Compared to the other forms, its habitat had the highest pH (4.71-4.92; Me = 4.71), the highest temperature and substrate hydration, and above all, the lowest photosynthetically active radiation (PAR; 20.4-59.4%). This form differed from the other forms in almost all of the features of the plant's architecture. It is particularly noteworthy that it had the largest main axis height among all of the forms, which exceeded 18 cm. The number of living leaves in a rosette was notable (18.1 ± 8.1), while the number of dead leaves was very low (6.9 ± 3.8). The most significant differences were in the shape of its submerged leaves, in which the length of the leaf blade was the lowest of all of the forms (0.493 ± 0.15 mm; p < 0.001) and usually the widest. The stem cross-sectional area was noticeably smaller in the submerged form than in the other forms, the xylem was less developed and collaterally closed vascular bundles occurred. Our analysis of the parameters of chlorophyll fluorescence in vivo revealed that the maximum quantum yield of the primary photochemistry of photosystem II is the highest for the submerged form (Me = 0.681), the same as the maximum quantum yield of the electron transport (Me φE
= 0.183). The efficiency of energy use per one active reaction center of photosystem II (RC) was the lowest in the submerged form (Me = 2.978), same as the fraction of energy trapped by one active RC (Me = 1.976) and the non-photochemical energy dissipation (DI
/RC; Me = 0.916). The ET
/RC parameter, associated with the efficiency of the energy utilization for electron transport by one RC, in the submerged plant reached the highest value (Me = 0.489). The submerged form of D. intermedia clearly differed from the emerged and peatland forms in its plant architecture. The submerged plants had a thinner leaf blade and less developed xylem than the other forms, however, their stems were much longer. The relatively high photosynthetic efficiency of the submerged forms suggests that most of the trapped energy is utilized to drive photosynthesis with a minimum energy loss, which may be a mechanism to compensate for the relatively small size of the leaf blade.
The short neuropeptide F (sNPF) family of peptides is a multifunctional group of neurohormones involved in the regulation of various physiological processes in insects. They have been found in a ...broad spectrum of species, but the number of isoforms in the precursor molecule varies from one to four. The receptor for sNPF (sNPFR), which belongs to the G protein-coupled receptor family, has been characterized in various insect orders and was shown to be an ortholog of the mammalian prolactin-releasing peptide receptor (PrPR). The sNPF signaling pathway interacts with other neurohormones such as insulin-like peptides, SIFamide, and pigment-dispersing factors (PDFs) to regulate various processes. The main physiological function of sNPF seems to be involved in the regulation of feeding, but the observed effects are species-specific. sNPF is also connected with the regulation of foraging behavior and the olfactory system. The influence of sNPF on feeding and thus energy metabolism may also indirectly affect other vital processes, such as reproduction and development. In addition, these neurohormones are involved in the regulation of locomotor activity and circadian rhythm in insects. This review summarizes the current state of knowledge about the sNPF system in insects.
Tachykinins (TKs) are a group of conserved neuropeptides. In insects, tachykinin-related peptides (TRPs) are important modulators of several functions such as nociception and lipid metabolism. ...Recently, it has become clear that TRPs also play a role in regulating the insect immune system. Here, we report a transcriptomic analysis of changes in the expression levels of immune-related genes in the storage pest Tenebrio molitor after treatment with Tenmo-TRP-7. We tested two concentrations (10
and 10
M) at two time points, 6 and 24 h post-injection. We found significant changes in the transcript levels of a wide spectrum of immune-related genes. Some changes were observed 6 h after the injection of Tenmo-TRP-7, especially in relation to its putative anti-apoptotic action. Interestingly, 24 h after the injection of 10
M Tenmo-TRP-7, most changes were related to the regulation of the cellular response. Applying 10
M Tenmo-TRP-7 resulted in the downregulation of genes associated with humoral responses. Injecting Tenmo-TRP-7 did not affect beetle survival but led to a reduction in haemolymph lysozyme-like antibacterial activity, consistent with the transcriptomic data. The results confirmed the immunomodulatory role of TRP and shed new light on the functional homology between TRPs and TKs.
Animal toxins can have medical and therapeutic applications. Principally, toxins produced by insects, arachnids, snakes and frogs have been characterized. Venomous mammals are rare, and their venoms ...have not been comprehensively investigated. Among shrews, only the venom of
has been analysed so far, and blarina toxin has been proven to be its main toxic component. It is assumed that
employs its venom to hunt larger prey. However, the toxic profile, properties and mode of action of its venom are largely unknown. Therefore, we analysed the cardio-, myo- and neurotropic properties of
venom and saliva of non-venomous
(control tests) in vitro in physiological bioassays carried out on two model organisms: beetles and frogs. For the first time, we fractionated
venom and
saliva by performing chromatographic separation. Next, the properties of selected compounds were analysed in cardiotropic bioassays in the
heart.
The venom of
caused a high decrease in the conduction velocity of the frog sciatic nerve, as well as a significant decrease in the force of frog calf muscle contraction. We also recorded a significant decrease in the frog heart contractile activity. Most of the selected compounds from
venom displayed a positive chronotropic effect on the beetle heart. However, one fraction caused a strong decrease in the
heart contractile activity coupled with a reversible cardiac arrest. We did not observe any responses of the insect heart and frog organs to the saliva of
. Preliminary mass spectrometry analysis revealed that calmodulin-like protein, thymosin β-10, hyaluronidase, lysozyme C and phospholipase A2 are present in the venom of
, whereas thymosin β4, lysozyme C and β-defensin are present in
saliva.
Our results showed that
venom has stronger paralytic properties and lower cardioinhibitory activity. Therefore, it is highly probable that
might use its venom as a prey immobilizing agent. We also confirmed that
is not a venomous mammal because its saliva did not exhibit any toxic effects.
An active heating system has been developed for application in smart clothing for mountain rescuers. It uses a set of sensors and is aimed at gathering necessary data for the elaboration and testing ...of an automatic control algorithm. The system is powered by a lithium-ion battery pack, which can be additionally charged from flexible PV modules. The article presents an estimation of this system’s power supply requirements and its energy budget. Since the system’s maximum operation time strongly depends on the efficiency of its main power converter, the design of the latter was based on a model enabling power loss estimation in its particular components. Characteristics ultimately measured on a prototype showed a high agreement with simulations. Furthermore, five different arrangements of PV modules were studied in order to find the most effective one. The system was tested in real conditions for the three most promising PV module configurations.
Vestibular impairments affect patients' movements and can result in difficulties with daily life activities. The main aim of this study is to answer the question whether a simple and short test such ...as rotation about a vertical axis can be an objective method of assessing balance dysfunction in patients with unilateral vestibular impairments. A 360˚ rotation test was performed using six MediPost devices. The analysis was performed in three ways: (1) the analytical approach based only on data from one sensor; (2) the analytical approach based on data from six sensors; (3) the artificial neural network (ANN) approach based on data from six sensors. For approaches 1 and 2 best results were obtained using maximum angular velocities (MAV) of rotation and rotation duration (RD), while approach 3 used 11 different features. The following sensitivities and specificities were achieved: for approach 1: MAV-80% and 60%, RD-69% and 74%; for approach 2: 61% and 85% and RD-74% and 56%; for approach 3: 88% and 84%. The ANN-based six-sensor approach revealed the best sensitivity and specificity among parameters studied, however one-sensor approach might be a simple screening test used e.g. for rehabilitation purposes.
Model organisms are often used in biological, medical and environmental research. Among insects,
, and
are often used. However, new model organisms still appear. In recent years, an increasing number ...of insect species has been suggested as model organisms in life sciences research due to their worldwide distribution and environmental significance, the possibility of extrapolating research studies to vertebrates and the relatively low cost of rearing. Beetles are the largest insect order, with their representative -
- being the first species with a completely sequenced genome, and seem to be emerging as new potential candidates for model organisms in various studies. Apart from
, additional species representing various Coleoptera families, such as
and many others, have been used. They are increasingly often included in two major research aspects: biomedical and environmental studies. Biomedical studies focus mainly on unraveling mechanisms of basic life processes, such as feeding, neurotransmission or activity of the immune system, as well as on elucidating the mechanism of different diseases (neurodegenerative, cardiovascular, metabolic, or immunological) using beetles as models. Furthermore, pharmacological bioassays for testing novel biologically active substances in beetles have also been developed. It should be emphasized that beetles are a source of compounds with potential antimicrobial and anticancer activity. Environmental-based studies focus mainly on the development and testing of new potential pesticides of both chemical and natural origin. Additionally, beetles are used as food or for their valuable supplements. Different beetle families are also used as bioindicators. Another important research area using beetles as models is behavioral ecology studies, for instance, parental care. In this paper, we review the current knowledge regarding beetles as model organisms and their practical application in various fields of life science.
Solanaceae glycoalkaloids (SGAs) possess cardiomodulatory activity.
This study investigated the potential interaction between verapamil and glycoalkaloids.
The cardioactivity of verapamil and ...glycoalkaloids (α-solanine and α-chaconine) was tested in adult beetle (Tenebrio molitor) myocardium in vitro using microdensitometric methods. The myocardium was treated with pure substances and mixtures of verapamil and glycoalkaloids for 9 min with saline as a control. Two experimental variants were used: simultaneous application of verapamil and glycoalkaloids or preincubation of the myocardium with one of the compounds followed by perfusion with a verapamil solution. We used 9 × 10
−6-5
× 10
−5
M and 10
−9
-10
−5
M concentration for verapamil and glycoalkaloids, respectively.
Verapamil, α-solanine and α-chaconine showed cardioinhibitory activity with IC
50
values equal to 1.69 × 10
−5
, 1.88 × 10
−7
and 7.48 × 10
−7
M, respectively. When the glycoalkaloids were applied simultaneously with verapamil, an antagonistic effect was observed with a decrease in the maximal inhibitory effect and prolongation of t
50
and the recovery time characteristic of verapamil. We also confirmed the expression of two transcript forms of the gene that encodes the α1 subunit of L-type calcium channels in the myocardium and brain with equal transcription levels of both forms in the myocardium and significant domination of the shorter form in the brain of the insect species tested.
The results show that attention to the composition of the daily diet during therapy with various drugs is particularly important. In subsequent studies, the nature of interaction between verapamil and SGAs on the molecular level should be checked, and whether this interaction decreases the efficiency of cardiovascular therapy with verapamil in humans.
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