In this study, the authors present an experimental and theoretical description of the use of first order Raman amplification to improve the performance of a Phase-sensitive optical time domain ...reflectometer (φOTDR) when used for vibration measurements over very long distances. A special emphasis is given to the noise which is carefully characterized and minimized along the setup. A semiconductor optical amplifier and an optical switch are used to greatly decrease the intra-band coherent noise of the setup and balanced detection is used to minimize the effects of RIN transferred from the Raman pumps. The sensor was able to detect vibrations of up to 250 Hz (close to the limits set by the time of flight of light pulses) with a resolution of 10 m in a range of 125 km. To achieve the above performance, no post-processing was required in the φOTDR signal. The evolution of the φOTDR signal along the fiber is also shown to have a good agreement with the theoretical model.
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•Polysulfone membranes doped with 5% w/w of graphene oxide (PS-GO) were prepared.•PS-GO membranes were used as adsorbant of organic contaminants from water.•PS-GO showed much higher ...efficiency with respect to pure PS and GO components.•PS-GO outperformed a commercial granular activated carbon at lower treatment times.
This work explored polysulfone (PS) – graphene oxide (GO) based porous membranes (PS-GO) as adsorber of seven selected organic contaminants of emerging concern (EOCs) including pharmaceuticals, personal care products, a dye and a surfactant from water. PS-GO was prepared by phase inversion method starting from a PS and GO mixture (5% w/w of GO). The porous PS-GO membranes showed asymmetric and highly porous micrometer sized pores on membrane top (diameter ≈20μm) and bottom (diameter ≈2–5μm) surfaces and tens of microns length finger like pores in the section. Nanomechanical mapping reveals patches of a stiffer material with Young modules comprised in the range 15–25GPa, not present in PS pure membranes that are compatible with the presence of GO flakes on the membrane surfaces. PS-GO was immersed in EOCs spiked tap water and the adsorbance efficiency at different contact times and pH evaluated by HPLC analysis. Ofloxacin (OFLOX), benzophenone-3 (BP-3), rhodamine b (Rh), diclofenac (DCF) and triton X-100 (TRX) were removed with efficiency higher than 90% after 4h treatments. Regeneration of PS-GO and reuse possibilities were demonstrated by washing with ethanol. The adsorption efficiencies toward OFLOX, Rh, DCF and carbamazepine (CBZ) were significantly higher than those of pure PS membrane. Moreover, PS-GO outperformed a commercial granular activated carbon (GAC) at low contact times and compared well at longer contact time for OFLOX, Rh, BP-3 and TRX suggesting the suitability of the newly introduced material for drinking water treatment.
Phase-sensitive optical time domain reflectometry (φOTDR) is a simple and effective tool allowing the distributed monitoring of vibrations along single-mode fibers. Up to now, φOTDRs have been used ...mostly for the measurement of sub-kHz vibrations, normally in the context of intrusion sensing. In this paper, the authors present an experimental and theoretical description of a high-visibility φOTDR and its performance when used for ultrasonic vibration measurements. The use of a semiconductor optical amplifier in the setup allows to suppress coherent noise and also to improve the spectral response of the pump pulses. These two advantages greatly decrease the detected intra-band noise thus allowing frequency measurements in the limits set by the time of flight of the light pulses while maintaining the simplicity of the scheme, as no post-processing, extremely high coherence lasers or coherent detection methods are required. The sensor was able to measure vibrations of up to 39.5 kHz with a resolution of 5 m over a range which could go up to 1.25 km. This is the first time to our knowledge that a fully distributed measurement of ultrasonic waves was achieved. The statistical behavior of the system was also described theoretically and characterized experimentally.
Steric blocking antisense oligonucleotides (ASO) are promising tools for splice modulation such as exon-skipping, although their therapeutic effect may be compromised by insufficient delivery. To ...address this issue, we investigated the synthesis of a 20-mer 2'-OMe PS oligonucleotide conjugated at 3'-end with ursodeoxycholic acid (UDCA) involved in the targeting of human
exon 51, by exploiting both a pre-synthetic and a solution phase approach. The two approaches have been compared. Both strategies successfully provided the desired ASO 51 3'-UDC in good yield and purity. It should be pointed out that the pre-synthetic approach insured better yields and proved to be more cost-effective. The exon skipping efficiency of the conjugated oligonucleotide was evaluated in myogenic cell lines and compared to that of unconjugated one: a better performance was determined for ASO 51 3'-UDC with an average 9.5-fold increase with respect to ASO 51.
We have compared the anti-proliferative activity in vitro, of R2SnGala (1-3) R = Me, n-Bu, Ph and novel R3SnGala (4, 5) R = Me, n-Bu with D-(+)-Galacturonic acid HGala; Galaq-, q = (2) and (1) for ...R2SnGala and R3SnGala, respectively compounds, towards human tumor cell lines of intestinal carcinoma (HCT-116) and breast adenocarcinoma (MCF-7). The new synthesized 4 and 5 compounds were characterized, in solution, by 1H, 13C and 119Sn NMR, that showed that HGala acts as monoanionic moiety and evidenced the dynamic behavior of the compounds, due to inter-conversions involving the anomeric carbon atom of the ligand. Cell viability, apoptosis induction and cell cycle distribution were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay and flow cytometry, respectively. The cytotoxicity of the compounds, in the micro-submicromolar range, changed in the order of the organotin(IV) moieties, according to 5 > 3 > 2, while 1 and 4, containing MenSn(IV) (n = 2,3) moieties, were ineffective. Compound 5 showed peculiar cytotoxic effects. It did not cause time dependent inhibition of cell growth nor accumulated into the cells. Cell death induced by the active 2, 3, and 5, was shown to be apoptotic by measuring the exposure of phosphatidylserine to the outer membrane and the loss of mitochondrial potential. All the cytotoxic compounds induced an accumulation of cells in the subG0/G1phase, while only 2 and 3 perturbed the cell cycle confining viable cells in G0/G1phase. Finally, none of the compounds investigated affected the viability of normal intestinal or liver cells, indicating selectivity towards tumor cells.
Di- and tri-organotin(IV) compounds with D-Galacturonic acid showed higher anti-proliferative activity, compared to cisplatin, towards MCF-7 or HCT-116 cell lines, while they were ineffective towards normal-like differentiated Caco-2 and Chang liver cells. Display omitted
•Two organotin(IV) compounds with D-(+)-galacturonic acid synthesized and characterized.•Antiproliferative activity of di- and tri-organotin(IV) – galacturonate compounds tested.•Superior activity of three of the tested compounds relative to cis-platin established.•The compounds studied are then proposed as promising organometallic-based drugs.
We present a fiber optic magnetic field sensor conceived for magnetic resonance imaging (MRI) applications. The sensor is based on the integration of fiber optic strain sensors (fiber Bragg ...gratings-FBGs) with a sensing material (Terfenol-D). The response of an FBG integrated with a block of Terfenol-D was preliminarily investigated by taking into account the dependence of the Terfenol-D magnetostrictive response on both the longitudinal and transversal magnetic fields, with different preloads. Based on the performed characterizations, a triaxial magnetic field sensor was designed, characterized, and fabricated. An algorithm enabling the demodulation of the magnetic field from the readout of the three FBGs was also implemented, by taking into account the interdependence among the different sensor responses. Experimental results demonstrate the ability of the triaxial sensor to measure the magnetic field. Performance assessment and critical analysis are reported as well, elucidating both the abilities and limitations of the implemented sensing configuration. Finally, as proof of principle, a sensing system constituted of 20 triaxial sensors has been fabricated and used to map the magnetic field strength distribution in an MRI diagnostic centre.
This paper summarizes some of our results on the application of oligothiophenes as fluorescent markers for biological studies. The oligomers of thiophene, widely known for their semiconductor ...properties in organic electronics, are also fluorescent compounds characterized by chemical and optical stability, high absorbance and quantum yield. Their fluorescent emission can be easily modulated via organic synthesis by changing the number of thiophene rings and the nature of side-chains. This review shows how oligothiophenes can be derivatized with active groups such as phosphoramidite, N-hydroxysuccinimidyl and 4-sulfotetrafluorophenyl esters, isothiocyanate and azide by which the (bio)molecules of interest can be covalently bound. This paper also describes how molecules such as oligonucleotides, proteins and even nanoparticles, tagged with oligothiophenes, can be used in experiments ranging from hybridization studies to imaging of fixed and living cells. Finally, a few multilabeling experiments are described.
Hepatocellular carcinoma (HCC) is the most common primary liver malignancy in adults and accounts for 85-90% of all primary liver cancer. Based on the estimation by the International Agency for ...Research on Cancer in 2018, liver cancer is the fourth leading cause of cancer death globally. Dihydroartemisinin (DHA), the main active metabolite of artemisinin derivatives, is a well-known drug for the treatment of malaria. Previous studies have demonstrated that DHA exhibits antitumor effects toward a variety of human cancers and has a potential for repurposing as an anticancer drug. However, its short half-life is a concern and may limit the application in cancer therapy. We have reported that UDC-DHA, a hybrid of bile acid ursodeoxycholic acid (UDCA) and DHA, is ∼12 times more potent than DHA against a HCC cell line HepG2. In this study, we found that UDC-DHA was also effective against another HCC cell line Huh-7 with an IC
of 2.16 μM, which was 18.5-fold better than DHA with an IC
of 39.96 μM. UDC-DHA was much more potent than the combination of DHA and UDCA at 1:1 molar ratio, suggesting that the covalent linkage rather than a synergism between UDCA and DHA is critical for enhancing DHA potency in HepG2 cells. Importantly, UDC-DHA was much less toxic to normal cells than DHA. UDC-DHA induced G0/G1 arrest and apoptosis. Both DHA and UDC-DHA significantly elevated cellular reactive oxygen species generation but with different magnitude and timing in HepG2 cells; whereas only DHA but not UDC-DHA induced reactive oxygen species in Huh-7 cells. Depolarization of mitochondrial membrane potential was detected in both HepG2 and Huh-7 cells and may contribute to the anticancer effect of DHA and UDC-DHA. Furthermore, UDC-DHA was much more stable than DHA based on activity assays and high performance liquid chromatography-MS/MS analysis. In conclusion, UDC-DHA and DHA may exert anticancer actions via similar mechanisms but a much lower concentration of UDC-DHA was required, which could be attributed to a better stability of UDC-DHA. Thus, UDC-DHA could be a better drug candidate than DHA against HCC and further investigation is warranted.
Optical cavities transmit light only at discrete resonant frequencies, which are well-separated in micro-structures. Despite attempts at the construction of planar 'white-light cavities', the ...benefits accrued upon optically interacting with a cavity - such as resonant field buildup - have remained confined to narrow linewidths. Here, we demonstrate achromatic optical transmission through a planar Fabry-Pérot micro-cavity via angularly multiplexed phase-matching that exploits a bio-inspired grating configuration. By correlating each wavelength with an appropriate angle of incidence, a continuous spectrum resonates and the micro-cavity is rendered transparent. The locus of a single-order 0.7-nm-wide resonance is de-slanted in spectral-angular space to become a 60-nm-wide achromatic resonance spanning multiple cavity free-spectral-ranges. The result is an 'omni-resonant' planar micro-cavity in which light resonates continuously over a broad spectral span. This approach severs the link between the resonance bandwidth and the cavity-photon lifetime, thereby promising resonant enhancement of linear and nonlinear optical effects over broad bandwidths in ultrathin devices.
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