The role of dietary protein source on growth performance and lipid metabolism in European sea bass (Dicentrarchus labrax) was investigated, by the evaluation of fat content in different tissues, ...plasma metabolites, liver lipogenic activity and fatty acid composition of dorsal muscle tissue.
Five isonitrogenous (490gkg−1 crude protein dry-matter basis) and isolipidic (170gkg−1 lipid dry-matter basis) diets with graded levels of wheat gluten, soybean and pea meal in substitution of fish meal were evaluated in European sea bass (initial body weight 23.9±0.1) for 96days. There was a significant difference (p<0.05) in dorsal muscle and liver lipid contents. Plasma glucose and cholesterol levels decreased significantly (p<0.05) as diet included soybean and wheat gluten meal (p<0.05). The composition of fatty acids in muscle triacylglycerols and phospholipids was affected by vegetable sources as n−6 PUFA percentage was significantly increased and n−3 PUFA percentage was significantly decreased (p<0.05), without modifying EPA/DHA ratio, when wheat gluten and pea meal were included in the diet. The results confirm that the quality and quantity of vegetable protein source could affect lipid metabolism and lipid content in the dorsal muscle of the European sea bass and suggest the possibility of manipulation of the diet for commercial purpose.
► We tested wheat gluten, soybean and pea as ingredients in European sea bass diets. ► The growth performance was not affected by vegetable ingredients. ► Lipid metabolism and tissue distribution were affected by vegetable ingredients. ► PCA of fatty acids allows to space out fish fed fish meal-based diet from fish fed wheat gluten-based diets. ► Dietary soybean decreased lipid, namely cholesterol, in sea bass muscle and plasma.
Few phenomena have been more disruptive to West European politics and society than the accumulative experience of post-WWII immigration. Against this backdrop spring two questions: Why have the ...immigrant-receiving states historically permitted high levels of immigration? To what degree can the social and political fallout precipitated by immigration be politically managed? Utilizing evidence from a variety of sources, this study explores the links between immigration and the surge of popular support for anti-immigrant groups; its implications for state sovereignty; its elevation to the policy agenda of the European Union; and its domestic legacies. It argues that post-WWII migration is primarily an interest-driven phenomenon that has historically served the macroeconomic and political interests of the receiving countries. Moreover, it is the role of politics in adjudicating the claims presented by domestic economic actors, foreign policy commitments, and humanitarian norms that creates a permissive environment for significant migration to Western Europe.
Purpose
Developing stable high concentration monoclonal antibody (mAb) formulations is increasingly important to move toward subcutaneous (SC) administration for better patient experience. Challenges ...stemming from protein–protein interactions in these crowded solutions, such as colloidal instability, limit the feasibility of some formulations because of concerns of safety, product quality, and/or manufacturability. Herein, we report novel random heteropolymer excipients that improve the colloidal stability of a high concentration mAb formulation for SC administration.
Methods
A library of polymers was synthesized and screened by a high-throughput, absorbance-based assay. The lead polymers were selected and characterized for their ability to alter the precipitation kinetics of a mAb in physiologically relevant conditions using two model systems.
Results
Biophysical testing via surface tension measurements, isothermal titration calorimetry (ITC), microscale thermophoresis (MST), and intrinsic fluorescence quenching indicated that the polymers delayed onset of mAb precipitation from a combination of surfactant behaviour and interactions with the protein to prevent protein–protein interactions leading to colloidal instability.
Conclusions
The random heteropolymers described are a new class of excipients that may enable development of SC mAb formulations previously inaccessible to patients.
Abstract Background More than 80% of infrarenal aortic aneurysms are treated by endovascular repair. However, adoption of fenestrated and branched endovascular repair for complex aortic aneurysms has ...been limited, despite high morbidity and mortality associated with open repair. There are few published reports of consecutive outcomes, inclusive of all fenestrated and branched endovascular repairs, starting from the inception of a complex aortic aneurysm program. Therefore, we examined a single center's consecutive experience of fenestrated and branched endovascular repair of complex aortic aneurysms. Methods This is a single-center, prospective, observational cohort study evaluating 30-day and 1-year outcomes in all consecutive patients who underwent fenestrated and branched endovascular repair of complex aortic aneurysms (definition: requiring one or more fenestrations or branches). Data were collected prospectively through an Institutional Review Board-approved registry and a physician-sponsored investigational device exemption clinical trial (G130210). Results We performed 100 consecutive complex endovascular aortic aneurysm repairs (November 2010 to March 2016) using 58 (58%) commercially manufactured custom-made devices and 42 (42%) physician-modified devices to treat 4 (4%) common iliac, 42 (42%) juxtarenal, 18 (18%) pararenal, and 36 (36%) thoracoabdominal aneurysms (type I, n = 1; type II, n = 4; type III, n = 12; type IV, n = 18; arch, n = 1). The repairs included 309 fenestrations, branches, and scallops (average of 3.1 branch arteries/case). All patients had 30-day follow-up for 30-day event rates: three (3%) deaths; six (6%) target artery occlusions; five (5%) progressions to dialysis; eight (8%) access complications; one (1%) paraparesis; one (1%) bowel ischemia; and no instances of myocardial infarction, paralysis, or stroke. Of 10 type I or type III endoleaks, 8 resolved (7 with secondary intervention, 1 without intervention). Mean follow-up time was 563 days (interquartile range, 156-862), with three (3%) patients lost to follow-up. On 1-year Kaplan-Meier analysis, survival was 87%, freedom from type I or type III endoleak was 97%, target vessel patency was 92%, and freedom from aortic rupture was 100%. Average lengths of intensive care unit stay and inpatient stay were 1.4 days (standard deviation, 3.3) and 3.6 days (standard deviation, 3.6), respectively. Conclusions These results show that complex aortic aneurysms can now be treated with minimally invasive fenestrated and branched endovascular repair. Endovascular technologies will likely continue to play an increasingly important role in the management of patients with complex aortic aneurysm disease.
This paper describes the development of a Horizontal Axis Wind Turbine 3D CFD model using the Ansys Fluent solver. The model was developed to predict wind turbine performance and evaluate the ...capabilities of the 1D model (based on BEM Theory) developed by the authors. The two models were compared in terms of accuracy, predictability and calculation time.
The strategy of generating a high quality mesh and optimizing the turbulence models (two equations SST k–ω fully turbulent and four equations Transitional SST models) is presented. In particular, a high quality unstructured 3D grid was generated to optimize spatial discretization and meet turbulence model requirements. The mesh was subsequently converted from a tetrahedral into a polyhedral geometry to considerably reduce the number of cells and better align the cell faces and flow. Polyhedral cells also reduce interpolation errors and false numerical diffusion. The empirical correlations of the Transitional SST turbulence model were modified to improve it for wind turbine applications. A significant number of numerical 2D airfoil tests were implemented to calibrate the turbulence model. The results of these tests were applied to the turbulence model by modifying the local correlation parameters. The same parameters were used in the 3D wind turbine model. A Moving Reference Frame model was used to simulate rotation and evaluate 3D flow along the rotor blades.
The numerical results were compared to the fully turbulent SST k–ω simulation data to demonstrate the superior capabilities of the modified Transitional model.
The 3D CFD model was validated using NREL PHASE VI experimental data available from scientific literature.
An application of the 3D model to a new micro wind turbine is presented at the end of this paper. The micro rotor was designed and optimized using the 1D code and actually built.
► A wind turbine CFD model was developed using an SST Transition turbulence model. ► Computational domain and mesh was optimized to obtain grid independence. ► A comparison between CFD model, 1D BEM model results and experimental data was done. ► An application of the 3D CFD model to a new micro wind turbine was presented.
We report results from searches for new physics with low-energy electronic recoil data recorded with the XENON1T detector. With an exposure of 0.65 tonne-years and an unprecedentedly low background ...rate of 76 ± 2stat events/(tonne × year × keVÞ between 1 and 30 keV, the data enable one of the most sensitive searches for solar axions, an enhanced neutrino magnetic moment using solar neutrinos, and bosonic dark matter. An excess over known backgrounds is observed at low energies and most prominent between 2 and 3 keV. The solar axion model has a 3.4σ significance, and a three-dimensional 90% confidence surface is reported for axion couplings to electrons, photons, and nucleons. This surface is inscribed in the cuboid defined by gae < 3.8 × 10−12, ..., and gaegaγ < 7.7 × 10−22 GeV−1, and excludes either gae = 0 or ... . The neutrino magnetic moment signal is similarly favored over background at 3.2σ, and a confidence interval of μν ∈ (1.4, 2.9) × 10−11 μB (90% C.L.) is reported. Both results are in strong tension with stellar constraints. The excess can also be explained by β decays of tritium at 3.2σ significance with a corresponding tritium concentration in xenon of (6.2 ± 2.0) × 10−25 mol/mol. Such a trace amount can neither be confirmed nor excluded with current knowledge of its production and reduction mechanisms. The significances of the solar axion and neutrino magnetic moment hypotheses are decreased to 2.0σ and 0.9σ, respectively, if an unconstrained tritium component is included in the fitting. With respect to bosonic dark matter, the excess favors a monoenergetic peak at (2.3 ± 0.2) keV (68% C.L.) with a 3.0σ global (4.0σ local) significance over background. This analysis sets the most restrictive direct constraints to date on pseudoscalar and vector bosonic dark matter for most masses between 1 and 210 keV/c2. We also consider the possibility that 37Ar may be present in the detector, yielding a 2.82 keV peak from electron capture. Contrary to tritium, the 37Ar concentration can be tightly constrained and is found to be negligible. (ProQuest: ... denotes formula omitted.)
People with type 2 diabetes mellitus (T2DM) have a 25-fold higher risk of limb loss than non-diabetics due in large part to impaired wound healing. Here, we show that the impaired wound healing ...phenotype found in T2D mice is recapitulated in lethally irradiated wild type recipients, whose hematopoiesis is reconstituted with hematopoietic stem cells (HSCs) from T2D mice, indicating an HSC-autonomous mechanism. This impaired wound healing phenotype of T2D mice is due to a Nox-2-dependent increase in HSC oxidant stress that decreases microRNA let-7d-3p, which, in turn, directly upregulates Dnmt1, leading to the hypermethylation of Notch1, PU.1, and Klf4. This HSC-autonomous mechanism reduces the number of wound macrophages and skews their polarization towards M1 macrophages. These findings reveal a novel inflammatory mechanism by which a metabolic disorder induces an epigenetic mechanism in HSCs, which predetermines the gene expression of terminally differentiated inflammatory cells that controls their number and function.
We report constraints on light dark matter (DM) models using ionization signals in the XENON1T experiment. We mitigate backgrounds with strong event selections, rather than requiring a scintillation ...signal, leaving an effective exposure of (22±3) tonne day. Above ∼0.4 keV_{ee}, we observe <1 event/(tonne day keV_{ee}), which is more than 1000 times lower than in similar searches with other detectors. Despite observing a higher rate at lower energies, no DM or CEvNS detection may be claimed because we cannot model all of our backgrounds. We thus exclude new regions in the parameter spaces for DM-nucleus scattering for DM masses m_{χ} within 3-6 GeV/c^{2}, DM-electron scattering for m_{χ}>30 MeV/c^{2}, and absorption of dark photons and axionlike particles for m_{χ} within 0.186-1 keV/c^{2}.
We report the first dark matter search results from XENON1T, a ∼2000-kg-target-mass dual-phase (liquid-gas) xenon time projection chamber in operation at the Laboratori Nazionali del Gran Sasso in ...Italy and the first ton-scale detector of this kind. The blinded search used 34.2 live days of data acquired between November 2016 and January 2017. Inside the (1042±12)-kg fiducial mass and in the 5,40 keV_{nr} energy range of interest for weakly interacting massive particle (WIMP) dark matter searches, the electronic recoil background was (1.93±0.25)×10^{-4} events/(kg×day×keV_{ee}), the lowest ever achieved in such a dark matter detector. A profile likelihood analysis shows that the data are consistent with the background-only hypothesis. We derive the most stringent exclusion limits on the spin-independent WIMP-nucleon interaction cross section for WIMP masses above 10 GeV/c^{2}, with a minimum of 7.7×10^{-47} cm^{2} for 35-GeV/c^{2} WIMPs at 90% C.L.
We report on the first search for nuclear recoils from dark matter in the form of weakly interacting massive particles (WIMPs) with the XENONnT experiment, which is based on a two-phase time ...projection chamber with a sensitive liquid xenon mass of 5.9 ton. During the (1.09±0.03) ton yr exposure used for this search, the intrinsic ^{85}Kr and ^{222}Rn concentrations in the liquid target are reduced to unprecedentedly low levels, giving an electronic recoil background rate of (15.8±1.3) events/ton yr keV in the region of interest. A blind analysis of nuclear recoil events with energies between 3.3 and 60.5 keV finds no significant excess. This leads to a minimum upper limit on the spin-independent WIMP-nucleon cross section of 2.58×10^{-47} cm^{2} for a WIMP mass of 28 GeV/c^{2} at 90% confidence level. Limits for spin-dependent interactions are also provided. Both the limit and the sensitivity for the full range of WIMP masses analyzed here improve on previous results obtained with the XENON1T experiment for the same exposure.
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