The positive impact of probiotic strains on human health has become more evident than ever before. Often delivered through food, dietary products, supplements, and drugs, different legislations for ...safety and efficacy issues have been prepared. Furthermore, regulatory agencies have addressed various approaches toward these products, whether they authorize claims mentioning a disease's diagnosis, prevention, or treatment. Due to the diversity of bacteria and yeast strains, strict approaches have been designed to assess for side effects and post-market surveillance. One of the most essential delivery systems of probiotics is within food, due to the great beneficial health effects of this system compared to pharmaceutical products and also due to the increasing importance of food and nutrition. Modern lifestyle or various diseases lead to an imbalance of the intestinal flora. Nonetheless, as the amount of probiotic use needs accurate calculations, different factors should also be taken into consideration. One of the novelties of this review is the presentation of the beneficial effects of the administration of probiotics as a potential adjuvant therapy in COVID-19. Thus, this paper provides an integrative overview of different aspects of probiotics, from human health care applications to safety, quality, and control.
Cadmium (Cd)-induced oxidative stress and hepatic injury is one of the major outcomes of chronic Cd toxicity, which can be ameliorated by numerous antioxidants. The present study was undertaken to ...find the therapeutic efficacy of naringenin (NGN) plus vitamins C and E on Cd-induced oxidative hepatotoxicity in Wistar rats. It has been noticed that Cd intoxication significantly elevates the levels of serum hepatic marker enzymes such as alanine amino transferase, aspartate amino transferase, alkaline phosphatase, lactate dehydrogenase, γ glutamyl transferase, total bilirubin, and hepatic thiobarbituric acid reactive substances, lipid hydroperoxides, conjugated dienes and protein carbonyls. In addition, Cd also decreases the activities of hepatic enzymatic antioxidants superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase and glucose-6-phosphate dehydrogenase and the levels of non-enzymatic antioxidants total sulphydryl groups, reduced glutathione, vitamins C and E and histopathological changes in liver. Treatment with NGN and vitamins C and E in combination more significantly improved the altered biochemical and histopathological changes in the liver of Cd-intoxicated rats than the NGN or vitamins C and E treatment alone. Conclusion: The present data suggest that combined administration of NGN with vitamins C and E proved to be more beneficial in the treatment of Cd-hepatotoxicity than NGN treatment alone.
The interest in the study of the gut microbiome has grown exponentially. Indeed, its impact on health and disease has been increasingly reported, and the importance of keeping gut microbiome ...homeostasis clearly highlighted. However, and despite many advances, there are still some gaps, as well as the real discernment on the contribution of some species falls far short of what is needed. Anyway, it is already more than a solid fact of its importance in maintaining health and preventing disease, as well as in the treatment of some pathologies. In this sense, and given the existence of some ambiguous opinions, the present review aims to discuss the importance of gut microbiome in homeostasis maintenance, and even the role of probiotics, prebiotics, and symbiotics in both health promotion and disease prevention.
Cadmium (Cd) induced oxidative stress and hepatic injury is one of the major outcomes of the chronic Cd toxicity, which can be ameliorated by numerous antioxidants. The present study was investigated ...to find out the therapeutic efficacy of naringenin (NGN) plus vitamins C and E on Cd induced oxidative hepatotoxicity in wistar rats. It has been noticed that Cd intoxication significantly elevated the levels of serum hepatic marker enzymes such as alanine amino transferase, aspartate amino transferase, alkaline phosphatase, lactate dehydrogenase, γ glutamyl transferase, total bilirubin, and hepatic thiobarbituric acid reactive substances, lipid hydro-peroxides, conjugated dienes and protein carbonyls. In addition, Cd also decreased the activities of hepatic enzymatic antioxidants superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase and glucose-6-phosphate dehydrogenase and the levels of non-enzymatic antioxidants total sulphydryl groups, reduced glutathione, vitamins C and E and histopathological changes in liver. Treatment with NGN and vitamins C and E combination significantly improved the altered biochemical and histopathological changes in the liver of Cd intoxicated rats than the NGN or Vitamins C and E treatment alone. Conclusion: The present data suggest that combined administration of NGN with vitamins C and E proved to be beneficial in the treatment of Cd-hepatotoxicity than NGN treatment alone.
Arsenic (As) is a naturally occurring semimetallic element that is classified as a toxicant and a human carcinogen. Diallyl trisulphide (DATS), an organosulphur compound, is an antioxidative ...substance that is extracted from garlic (Allium sativum). Erythrocytes are very expedient models to understand the susceptibility of membrane to oxidative damage induced by different xenobiotic compounds. Arsenic has been reported to induce oxidative stress to erythrocytes due to lipid peroxidation and alteration in defence mechanism as erythrocytes are the first target that arsenic compounds attack in the body after systemic absorption. In the light of this fact, the purpose of this study is to characterise the ameliorative effect of DATS on arsenic-induced oxidative stress in rat erythrocytes.
Experimental rats were randomly divided into four groups and treated orally for 28 days: control, As 5 mg/kg body weight (BW) treated, As+DATS (80 mg/kg BW) treated, DATS (80 mg/kg BW) treated and As+vitamin C (100 mg/kg BW) treated. Oxidative stress in erythrocytes was recorded by estimating plasma marker enzymes, plasma and erythrocyte membrane oxidative stress markers, erythrocyte membrane antioxidant enzymes and non-antioxidant enzymes, etc.
Oral administration of arsenic at 5 mg/kg BW per day elevated the levels of plasma marker enzymes, namely, aspartate transaminase (AST), alanine transaminase (ALT), acid phosphatase (ACP), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and γ-glutamyl transferase (γGT) (U/L) with significantly increased lipid peroxidation markers such as thiobarbituric acid reactive substances (TBARS), malondialdehyde (MDA), lipid hydroperoxides (LH), conjugated dienes (CD), and protein carbonyl (PC) contents were also elevated in As-treated rat plasma and erythrocytes. The levels of non-enzymatic antioxidants (reduced glutathione, vitamins C and E) and enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G6PD) were also decreased in As-treated rats. The toxic effect of As significantly decreased the activities of membrane-bound ATPases (Na+/K+-ATPase, Mg2+-ATPase, and Ca2+-ATPase), with a significant increase in% tail DNA of rat lymphocytes measured by means of a single-cell gel electrophoresis assay. Administration of DATS for 28 days significantly reduced the levels of plasma markers. The levels of TBARS, MDA, LH, CD, and PC were significantly decreased and there was a significant increase in ATPase activities and non-enzymatic and enzymatic antioxidants on treatment with DATS in a dose-related manner.
All these changes were supported by reduction of DNA damage in lymphocytes with DATS treatment. DATS at a dose of 80 mg/kg BW was found to be most effective and the results revealed the same. The results of the study showed that DATS shows a protective effect against As-induced oxidative stress in rat erythrocytes and lymphocytes.
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