Context.
It has become clear in recent years that binarity plays a crucial role in many aspects of planetary nebulae (PNe), particularly with regard to the striking morphologies they exhibit. To ...date, there are nearly 60 known binary central stars of PNe (bCSPNe). However, both theory and observation indicate that this figure represents only the tip of the iceberg, with the Galactic PN population hosting orders of magnitude more stars.
Aims.
We are involved in a search for new bCSPNe with the aim of enhancing the statistical validation of the key role of binarity in the formation and shaping of PNe. New discoveries of bCSPNe and their characterization carry important implications not only for understanding PN evolution, but also for studying binary evolution and the common-envelope phase, which is still poorly understood.
Methods.
We used data from the TESS satellite to search for variability in the eight CSPNe that belong to the two-minute cadence of preselected targets in Cycle 1, with their available pipeline-extracted light curves. We identified strong periodicities and analysed them in the context of the binary scenario.
Results.
All the CSPNe but one (Abell 15) show clear signs of periodic variability in TESS. The cause of this variability can be attributed to different effects, some of them requiring the presence of a companion star. We find simple sinusoidal modulations in several of the systems, compatible with irradiation effects. In addition, two of the central stars (PG 1034+001 and NGC 5189) also show photometric variations due to ellipsoidal variations and other signs of variability that are probably caused by star spots or relativistic Doppler-beaming. The case of the well-studied Helix Nebula is of particular interest; here we constructed a series of binary models to explain the modulations we see in the light curve. We find that the variability constrains the possible companion to be very low-mass main-sequence star or sub-stellar object. We also identify, in substantial detail, the individual pulsation frequencies of NGC 246.
Acetylcholine (ACh) plays a crucial role in physiological responses of both the central and the peripheral nervous system. Moreover, ACh was described as an anti-inflammatory mediator involved in the ...suppression of exacerbated innate response and cytokine release in various organs. However, the specific contributions of endogenous release ACh for inflammatory responses in the lung are not well understood. To address this question we have used mice with reduced levels of the vesicular acetylcholine transporter (VAChT), a protein required for ACh storage in secretory vesicles. VAChT deficiency induced airway inflammation with enhanced TNF-α and IL-4 content, but not IL-6, IL-13 and IL-10 quantified by ELISA. Mice with decreased levels of VAChT presented increased collagen and elastic fibers deposition in airway walls which was consistent with an increase in inflammatory cells positive to MMP-9 and TIMP-1 in the lung. In vivo lung function evaluation showed airway hyperresponsiveness to methacholine in mutant mice. The expression of nuclear factor-kappa B (p65-NF-kB) in lung of VAChT-deficient mice were higher than in wild-type mice, whereas a decreased expression of janus-kinase 2 (JAK2) was observed in the lung of mutant animals. Our findings show the first evidence that cholinergic deficiency impaired lung function and produce local inflammation. Our data supports the notion that cholinergic system modulates airway inflammation by modulation of JAK2 and NF-kB pathway. We proposed that intact cholinergic pathway is necessary to maintain the lung homeostasis.
Rabies is a fatal viral zoonosis caused by rabies virus (RABV). RABV infects the central nervous system and triggers acute encephalomyelitis in both humans and animals. Endemic in the Brazilian ...Northeast region, RABV emergence in distinct wildlife species has been identified as a source of human rabies infection and as such, constitutes a public health concern. Here, we performed post‐mortem RABV analyses of 144 encephalic tissues from bats sampled from January to July 2022, belonging to 15 different species. We identified phylogenetically distinct RABV from Phyllostomidae and Molossidae bats circulating in Northeastern Brazil. Phylogenetic clustering revealed the close evolutionary relationship between RABV viruses circulating in bats and variants hosted in white‐tufted marmosets, commonly captured to be kept as pets and linked to human rabies cases and deaths in Brazil. Our findings underline the urgent need to implement a phylogenetic‐scale epidemiological surveillance platform to track multiple RABV variants which may pose a threat to both humans and animals.
Cell-based assays using three-dimensional (3D) cell cultures may reflect the antitumor activity of compounds more accurately, since these models reproduce the tumor microenvironment better.
Here, we ...report a comparative analysis of cell behavior in the two most widely employed methods for 3D spheroid culture, forced floating (Ultra-low Attachment, ULA, plates), and hanging drop (HD) methods, using the RT4 human bladder cancer cell line as a model. The morphology parameters and growth/metabolism of the spheroids generated were first characterized, using four different cell-seeding concentrations (0.5, 1.25, 2.5, and 3.75 × 10
cells/mL), and then, subjected to drug resistance evaluation.
Both methods generated spheroids with a smooth surface and round shape in a spheroidization time of about 48 h, regardless of the cell-seeding concentration used. Reduced cell growth and metabolism was observed in 3D cultures compared to two-dimensional (2D) cultures. The optimal range of spheroid diameter (300-500 μm) was obtained using cultures initiated with 0.5 and 1.25 × 10
cells/mL for the ULA method and 2.5 and 3.75 × 10
cells/mL for the HD method. RT4 cells cultured under 3D conditions also exhibited a higher resistance to doxorubicin (IC
of 1.00 and 0.83 μg/mL for the ULA and HD methods, respectively) compared to 2D cultures (IC
ranging from 0.39 to 0.43).
Comparing the results, we concluded that the forced floating method using ULA plates was considered more suitable and straightforward to generate RT4 spheroids for drug screening/cytotoxicity assays. The results presented here also contribute to the improvement in the standardization of the 3D cultures required for widespread application.
In this work, the quasi-analog to discrete transition occurring in the current-voltage characteristic of oxygen engineered yttrium oxide-based resistive random-access memory (RRAM) devices is ...investigated in detail. In particular, the focus of our research is not on the absolute conductance values of this characteristic but on the magnitude of its conductance changes occurring during the reset process of the device. It is found that the detected changes correspond to conductance values predominantly of the order of the quantum unit of conductance G
= 2e
/h, where e is the electron charge and h the Planck constant. This feature is observed even at conductance levels far above G
, i.e. where electron transport is seemingly diffusive. It is also observed that such behavior is reproducible across devices comprising yttrium oxide layers with different oxygen concentrations and measured under different voltage sweep rates. While the oxygen deficiency affects the total number of quantized conductance states, the magnitude of the changes in conductance, close to 1 G
, is invariant to the oxygen content of the functional layer.
Abstract
We report the discovery of a new emission-line object, named SPH 4-South = (GAIA EDR3 5616553300192230272), toward the dark cloud LDN 1667. This object came to our attention after inspecting ...public images that show a faint diffuse nebula a few arcseconds south of SPH 4, an emission-line object previously classified as a T Tauri star. We present high-resolution spectra and analyzed
JHK
photometry of SPH 4 and SPH 4-South and new narrowband and archival broadband images of these objects. A comparison of the spectra of SPH 4 and SPH 4-South with high-resolution ones of DG Cir and R Mon strongly suggests that SPH 4 and SPH 4-South are Herbig Ae/Be stars. The classification of SPH 4-South is further supported by using a
k
-NN algorithm to its position in an
H
−
K
versus
J
−
H
color–color diagram. Both stars are detected in the four WISE bands and the WISE colors allow us to classify SPH 4 as a Class I and SPH 4-South as a Class II source. We also show that the faint nebula is most probably associated with SPH 4-South. Using published results on LDN 1667 and the Gaia Early Data Release 3, we conclude that SPH 4 is a member of LDN 1667. The case of SPH 4-South is not clear because the determination of its distance and proper motion could be affected by the nebulosity around the star, although membership of SPH 4-South to LDN 1667 cannot be ruled out.
Leishmania parasites have an oxidative and chemical defense mechanism called trypanothione system (TSH2), the most abundant thiol system in trypanosomatids. This system has a central role in ...processing pentavalent antimony and resistance has been related to a better capacity to metabolize it through the activation of TSH2 enzymatic cascade. A biochemical approach was applied to assess the effect of trivalent (SbIII) and pentavalent antimony (SbV) on Trypanothione Reductase (TR) activity of two Leishmania (Viannia) braziliensis clinical isolates, which were labeled as responder (R) and non-responder (NR) after patient treatment with Glucantime®. Both isolates were characterized based on in vitro susceptibility to SbIII and SbV and trypanothione reductase (TR) activity. SbIII and SbV discriminated susceptibility profiles in all parasite forms, since isolate NR had significantly higher EC50 values than isolate R. Differences were observed in TR activity between promastigotes, axenic amastigotes and intracellular amastigotes: R (0.439 ± 0.009, 0.103 ± 0.01 and 0.185 ± 0.01AU.min−1.μg of protein−1) and NR (1.083 ± 0.04, 0.914 ± 0.04 and 0.343 ± 0.04 AU. min−1.μg of protein−1), respectively. Incubation with SbIII and SbV using each form EC50 value caused a time-dependent differential effect on TR activity suggesting that oxidative defense is related to the antimony susceptibility phenotype. Data gathered here shows a biochemical approach able to discriminate two L. (V.) braziliensis clinical isolates measurements TR activity of promastigotes, axenic amastigotes and intracellular amastigotes.
•L.(V.)braziliensis forms have different Trypanothione Reductase (TR) activities.•TR activity is different according to antimony-response phenotype.•Antimony influence the TR activity according to antimony-response phenotype.•Axenic amastigotes are a feasible model to relate TR activity with antimony-response.
We present narrow-band optical and near-IR imaging and optical long-slit spectroscopic observations of Hu 1-2, a Galactic planetary nebula (PN) with a pair of N ii-bright, fast-moving (>340 km s−1) ...bipolar knots. Intermediate-dispersion spectra are used to derive physical conditions and abundances across the nebula, and high-dispersion spectra to study the spatio-kinematical structure. Generally, Hu 1-2 has high He/H (≈0.14) and N/O ratios (≈0.9), typical of Type I PNe. On the other hand, its abundances of O, Ne, S, and Ar are low as compared with the average abundances of Galactic bulge and disc PNe. The position–velocity maps can be generally described as an hour-glass shaped nebula with bipolar expansion, although the morphology and kinematics of the innermost regions cannot be satisfactorily explained with a simple, tilted equatorial torus. The spatio-kinematical study confines the inclination angle of its major axis to be within 10° of the plane of sky. As in the irradiated bow-shocks of IC 4634 and NGC 7009, there is a clear stratification in the emission peaks of O iii, Hα, and N ii in the north-west (NW) knot of Hu 1-2. Fast collimated outflows in PNe exhibit higher excitation than other low-ionization structures. This is particularly the case for the bipolar knots of Hu 1-2, with He ii emission levels above those of collimated outflows in other Galactic PNe. The excitation of the knots in Hu 1-2 is consistent with the combined effects of shocks and UV radiation from the central star. The mechanical energy and luminosity of the knots are similar to those observed in the PNe known to harbour a post-common envelope (post-CE) close binary central star.
ABSTRACT Water fountains are evolved stars showing early stages of collimated mass-loss during transition from the asymptotic giant branch, providing valuable insight into the formation of asymmetric ...planetary nebulae. We report the results of multi-epoch VLBI observations, which determine the spatial and three-dimensional kinematic structure of H2O masers associated with the water fountain IRAS 18113−2503. The masers trace three pairs of high-velocity (∼150–300 km s−1) bipolar bow shocks on a scale of 0${^{\prime\prime}_{.}}$18 (∼2000 au). The expansion velocities of the bow shocks exhibit an exponential decrease as a function of distance from the central star, which can be explained by an episodic, jet-driven outflow decelerating due to drag forces in a circumstellar envelope. Using our model, we estimate an initial ejection velocity ∼840 km s−1, a period for the ejections ∼10 yr, with the youngest being ∼12 yr old, and an average envelope density within the H2O maser region $n_{\text{H}_2}{\approx }10^{6}$ cm−3. We hypothesize that IRAS 18113−2503 hosts a binary central star with a separation of ∼10 au, revealing novel clues about the launching mechanisms of high-velocity collimated outflows in water fountains.
PDF
