The goal of this study was a molecular genetic passportization of the initial breeding material of sugar beet (male sterile lines, synanthous pollinators, and their hybrids). The following SSR ...primers were used: Unigene 24 552, Unigene 2305, Unigene 17 623, Unigene 14 805, and Unigene 62 524. The length range of the obtained DNA fragments varied within 100–3000 bp, and up to 11 polymorphic bands per genotype were amplified. The maximum Polymorphic Information Content (PIC) value was revealed for the Unigene 17 623 (PIC = 0.88), Unigene 2305 (PIC = 0.84), and Unigene 14 805 (PIC = 0.85) loci that provided a possibility to differentiate sugar beet breeding material. Using five of the tested SSR markers, the molecular genetic passportization of 26 genotypes of valuable breeding samples of this crop was carried out that allowed their identification and certification for further use in a marker-assisted selection. The calculated genetic distances between male sterile forms and synanthous pollinators varied from 2.236 to 4.796. Parent samples located at considerable genetic distances from each other were recommended for use in creating heterotic hybrids.
Article
—This article describes the construction, principles of operating, and characteristics of the TOTEM- electron spectrometer developed for the Strannik complex of scientific instruments for the
...Resonance
-
MKA
space project. In the article, analytical characteristics are presented of the qualification model of the instrument, which fully corresponds to the flight unit, and the procedure for functional tests of the instrument is described. The article also describes the structure and principles of functioning of the hardware and software laboratory facility developed for ground calibrations and tests of such a type of instruments. The design of the TOTEM-E instrument offers a new approach to measuring particle fluxes, which allows the accuracy and speed of measurements to be increased. A feature of the proposed scheme is the possibility of simultaneous measurement of electron fluxes in a plane section in the velocity space in the energy range from
E
0
to 6.5 ×
E
0
, where
E
0
is the minimum particle energy recorded by the instrument. This is achieved by using two conical electrostatic mirrors that take electrons from a flat 360° flow section for subsequent energy analysis and using a coordinate-sensitive detector for simultaneous particle registration.
PDF
