Drinking water that is secure to drink is a basic human need that should be met by every-one. Waterborne disease prevention and control begin with ensuring the safety of drinking water. The present ...study was designed to evaluate the physical and chemical quality of ground water in 20 different localities of Tehsil Taunsa DG Khan, Punjab Pakistan. For this purpose, ground water samples were collected from Mangrotha, Sokar, BastiBuzdar, KotQaisrani, JhokeBodo, Litra, Vehova, Bohar, MakwalKalan, Bindi, Dona, NariJanubi, TibbiQaisrani, Morejhangi, Babbi, Nutkani, JaluWali, Lakhani, Kotani and Chulani were physico-chemically (Turbidity, Odor, Color, TDS, HCO3, Alkalinity, Ca, CO3, Cl, EC, Fl, Hardness, Mg, SO4, pH, Na and K) characterized. The results revealed a significant increase in physical and chemical counts. Among Chemical parameters, the concentration of Alkalinity was in range of 205 mg/L to 235 mg/L, the concentration of Calcium was in range of 60 mg/L to 112 mg/L, the concentration of carbonates only in one location (BastiBuzdar) was slightly high 64 mg/L, the value of Electrical Conductivity was in range of 1230 µS/cm to 1260 µS/cm, the concentration of hardness varies between 145 mg/L to 550 mg/L. The levels of all other parameters were within WHO's acceptable ranges. A survey was also done to assess the impact of drinking water on city inhabitants' fitness. Globally, 780 million people, and 100 million people in Pakistan are exposed to insecure water sources. To estimate the health threat of infected water, a total of 160 residents interviewed. The information acquired from this field work will reveal a high prevalence of suspected water borne diseases like diarrhea, nausea & vomiting, gastrointestinal issues, Skin Rash, Skin irritation, Diabetes, Neural diseases, Renal dysfunction, Cholera, malaria, prolonged fever, Cancer, Hepatitis and jaundice. To resolve water and environmental problems, consciousness and regular monitoring programs of water organization and safe removal of waste was proposed. As a result, in all of the research areas, a well-organized waste disposal and management system is required. To make sure that the water is suitable for human consumption, regular drinking water quality assessments of the resource, main allotment tanks, distribution systems and pipes should be used.
sp. (Rhabdiasidae) is a nematode parasite of family Rhabdiasidae infecting the lung of amphibians. The present study provides new morphological details for
isolated from the lungs of the African ...common toad,
based on observations of light and scanning electron microscopy (SEM).
Forty specimens were collected from its natural habitat: the damp, moist fields and gardens at Giza governorate, Egypt. Worms were isolated from the lungs, fixed and then preserved. They were examined using light and SEM with further line drawings.
Fourteen specimens (35%) were found to harbor
with an intensity of three to five worms per host. Bodies of the gravid females were elongated, slender, measured 3.22-9.86 (5.64 ± 0.03) long and 0.09-0.48 (0.23 ± 0.02) wide at mid-length. Buccal capsule was with cylindrical lumen and sclerotized walls. Ovaries were almost straight. The uteri were located anterior and posterior to the vulva. Uterus were filled with a large number of eggs (17-42). Eggs were oval in shape and some of them were with fully developed larvae inside. The tail was comparatively short, gradually tapered. SEM showed that worms possessed an oval anterior end with a simple, slit like oral opening. The lipless edges of the mouth opening were bordered with tiny cuticular elevations, radiated outwards. Two pairs of submedian cephalic papillae were observed around the oral opening as well as two amphids.
The current study presents new morphological details for
isolated from the African common toad. Also, the morphology of the slit-like mouth opening, the two pairs of cephalic papillae, two amphids and the three pairs of cuticular elevations supporting the area around mouth opening were investigated.
Forty-three out of 120 (35.8 %)
Clarias garipenus
fish were found to be naturally infected with
Henneguya suprabanchiae
. The infection appeared as clusters of ovoid to ellipsoidal plasmodia being ...embedded within the hyaline cartilage of the suprabranchial organ of the fish. Histological studies indicate tissue distortion at the sites of infection. The continuous growth of the plasmodium led to deformation of the filament structure as well as disorganization and displacement of the gill lamellae. Also, a severe atrophy occurred in the hyaline cartilage of the gills and also in the gill lamellae. Based on the structure and measurements of fresh spores by light microscopy, this parasite was identified as
H. suprabranchiae.
Spores are oval in shape, and they measure 13 (11–14) × 3 (2–5) μm length by width. It has two polar capsules inside, each measures 3 (2.5–5) × 1 (1.5–4) μm length by width. Each polar capsule has a spirally coiled (7–9 turns) polar filament. The ultrastructural analysis showed that the plasmodia were surrounded by single-unit membrane. The generative cells and the early developmental stages were arranged at the periphery of the plasmodia, while immature and mature spores were centrally arranged. The developmental stages characterizing sporogenesis, capsulogenisis, and valvogenesis of the present parasite were ultrastructurally studied. The small subunit 18 S rDNA (SSU rDNA) gene sequences from different isolates was sequenced and compared with the sequence of the same gene from the
Henneguya
sp. isolated from GenBank. The phylogenetic position of the present
Henneguya
sp. within the genus was determined using sequence analysis of all related taxa available in GenBank, and the phylogenetic tree derived from this study is elucidated and compared with the current taxonomy of the available myxosporeans. Comparison of the nucleotide sequences and divergence showed that the SSU rDNA gene of this
Henneguya
species revealed 92.2 % sequence identity with
Henneguya exilis
(acc. no. AF021881) differing in 30 nucleotides with lower divergence value.
Colorectal cancer is reported as the third major incentive of cancer doom. The present work is meant to examine the anticancer potential of Origanum majorana leaf acetone extract (OMAE) to fight ...HT-29 human colon cancer cells. Biomolecules in OMAE were examined using Fourier Transform Infrared (FT-IR) spectroscopy. Reactive oxygen species (ROS) in OMAE was determined using an immunosobant assay. The cytotoxic effect of OMAE was tested by MTT assay. p53 gene expression level of OMAE-tread cells was measured using quantitative real-time PCR (qPCR). Apoptotic and cell cycle arrest effects of OMAE on HT-29 were analyzed by flow cytometry. Results revealed the presence of many functional groups and considerable amount of ROS in the extract. The extract could raise p53 expression level five folds over control. OMAE arrested the HT-29 at G2/M phase. OMAE has an apoptotic effect rather than necrotic effects Our discoveries give solid proof that O. majorana acetone extract has a capacity to stop colon cancer activity, at least, through the enlistment of cell division arrest as well as apoptosis. These findings can suggest the use of OMAE as a natural therapeutic candidate against the colon cancer.
The growing global demand for energy, particularly petroleum-based fuels, has stimulated a long-term quest for an optimal source of sustainable energy. This barrier is removed by lignocellulosic ...biomass, which is an economical, easily accessible, and renewable fuel source that fits sustainability standards. However, large-scale use of most of the techniques results in significant handling costs and decontamination of the inhibitors released. Taken together, these limits increase the efficacy of present solutions and create a need for the development of a novel, environmentally sustainable, productive, and cost-effective technology for lignocellulose biomass conversion. In this context, the use of nanotechnology in the treatment of lignocellulose biomass to bioenergy exchange has gained significant attention and has been extensively researched in recent years. This review discussed how nanotechnology can be used to turn biomass into energy. It gives new ideas and tools for developing new industries, which will help the economy, grow in the long run. This careful examination will also shed light on some of the minor details surrounding the different ways of biomass conversion previously explored by other experts.
is a heteroxynematid nematoda infecting most of the laboratory animals, occasionally mice which represent the mostly used animal for biological, medical, and pharmacological studies. The present ...study aimed to investigate the prevalence of nematode parasites infection in the laboratory mice
in Egypt. Morphologically, this oxyurid possessed four distinct cephalic papillae on a cephalic plate, with three small rudimental lips carrying two sessile poorly developed labial papillae and one pair of amphidial pores. Esophagus divided into cylindrical corpus and globular bulb. Distinct cervical alae interrupted at the level of esophago–intestinal junction forming an acute angle. At the caudal end, twelve caudal papillae in male worms while an ovijector apparatus opening and a vulva surrounded by protruded lips in females were observed. The general morphological criteria include this nematode with other
species which were compared in the present study. Molecular characterization based on 18SSU rDNA sequencing performed to confirm the taxonomic position of this species and to documents the morphological data. Sequence alignment detects a percent of identity up to 88.0% with other Heteroxynematidae species. Phylogenetic analysis showed that the present recorded is a putative sister taxon to
recorded in a previous study. The SSU rDNA sequence has been deposited in the GenBank under the accession no. MG019400.
The consumption of inadequately thermally treated fish is a public health risk due to the possible propagation of Anisakis larvae. The present study demonstrated the physiological and ...histopathological changes that accompanied an oral inoculation of crude extracts from fresh and thermally treated Anisakis Type II (L3) in rats. Worms were isolated from a marine fish and examined and identified using light and scanning electron microscopy. The study was performed in 6 rat groups: control (I), garlic oil (GO) inoculated (II), fresh L3 inoculated (III), thermally treated L3 inoculated (IV), fresh L3 + GO inoculated (V), and a thermally treated L3 + GO inoculated (VI) groups. Rats inoculated with fresh and thermally treated L3 showed abnormal liver and kidney functions associated with the destruction of normal architecture. GO produced a protective effect in rat groups inoculated with L3 extracts + GO via the amelioration of liver and kidney functions, which was confirmed by the marked normal structure on histology. Cooking of L3-infected fish induced severe alterations compared to uncooked fish. The administration of garlic before and after fish eating is recommended to avoid the dangerous effect of anisakids, even if they are cooked.
The present study evaluated the possible ameliorative efficacy of selenium nanoparticles (SeNPs) on AlCl
3
-induced hepatorenal injury in rats. Animals were randomly divided into four groups (
n
= ...6): group 1, the control; group 2, received SeNPs (0.4 mg/kg b.wt) for 21 days; group 3, injected with three doses of AlCl
3
intraperitoneally (30 mg/kg/body weight) every 5 days; group 4, received SeNPs for 7 days prior to AlCl
3
and then received SeNPs concurrently with AlCl
3
for the following 14 days. It was observed that AlCl
3
increased the levels of AST, ALT, ALP, LDH, total bilirubin, creatinine, urea, uric acid, and MDA significantly; as well as the reduction in the levels of GSH, SOD, GPx stores in comparison with the control group. These biochemical alterations were accompanied and confirmed by the lesion appeared in histological sections in addition to the increase in the expression of caspase-3 and the decrease of the Bcl-2expression. Treatment with SeNPs ameliorates the hepatorenal dysfunction, replenishes the endogenous antioxidant system, downregulates the expression of caspase-3, and upregulates the expression of Bcl-2. This hepatorenal ameliorative role may be due to the ability of SeNPs to equilibrate the oxidant/antioxidant system besides its ability to attenuate apoptosis process.
Monogenea is a class of ectoparasitic flatworms on the skin, gills, or fins of fish. Microcotylidae is a family of polyopisthocotylean monogeneans parasitising only marine fishes. This work describes ...and taxonomically determines a microcotylid polyopisthocotylean monogenean in an important fish in Saudi aquaculture.
Thirty gilt-head sea bream captured alive from the Red Sea of Saudi Arabia were examined for monogenean infection. Worms were described morphologically and morphometrically by light microscopy and multiple sequence alignments and phylogenetic trees were also constructed after maximum likelihood analysis of the 28S rRNA sequences.
Seventeen fish were infected by a monogenean parasite in the gill lamellae. It showed a bilobed anterior extremity, two rows of numerous unequal clamps of microcotylid type, and paired muscular vaginae crowned by differently sized spines. The vaginal number and its relative armature suggested the species' affiliation to group D; the parasite possessed large, muscular vaginae with a full corona of spines over almost the entire width resembling
Murray (1931). The molecular analysis of the parasite 28s rRNA revealed 97% homology with
(AJ577461.1).
The results confirmed the taxonomic status of the parasite recorded. On the basis of morphology and molecular data, we consider that several conclusions on the systematic status of microcotylids from Red Sea fishes in Saudi Arabia should be discussed.
Background. Malaria is still a public health problem in Saudi Arabia specifically in the Jazan region. Plasmodium falciparum knob-associated histidine-rich proteins (PfKAHRPs) play an important role ...in cerebral malaria pathophysiology as well as pathogenesis of P. falciparum infections. The repeat region of PfKAHRP C-terminal interaction domain has been found to bind to the infected red blood cells and the vascular endothelium. Thus, this study aimed to assess the allelic variations, genetic diversity, and natural selection acting at the C-terminal PfKAHRP between parasite isolates from Saudi Arabia. Materials and Methods. The PfKHARP C-terminal interaction domain was successfully PCR-amplified and sequence data from 441 clinical isolates from Saudi Arabia were obtained. The DnaSP v5.10 software was used to determine the genetic diversity, polymorphism, haplotype, and natural selection. Haplotype network analysis was constructed by using the median-joining method in the NETWORK version 5.0.0.1 software. Results. Alignment and analysis of 441 C-terminal PfKAHRP-deduced amino acid sequences identified 5 genotypes (I–V) based on the decapeptide repeat arrangements (TKEASTSKEA, TKEASTSKGA, TKEASTTEGA, and TKEASTSKRA). Among the repeat types, Type I (49.43%, 218/441) was the most abundant in Saudi Arabia, followed by Type II (48.29%, 213/441). Overall, the nucleotide diversity in the PfKHARP C-terminal region was found to be low in Saudi Arabia (π = 0.00142); however, natural selection tests indicated positive selection (dN-dS = 1.64, P<0.05) which was due to the variations within the repeat motifs. Genealogical relationship haplotype network of PfKAHRP from 4 different countries (i.e., Saudi Arabia, Iran, Burundi, and India) revealed 1 major shared haplotype cluster (H_1) with samples representative from all 4 countries (Saudi Arabia; n = 441, Burundi; n = 4, Iran; n = 13, and India; n = 1). Conclusion. Since this is the first study to report on genetic diversity of C-terminal PfKAHRP interaction domain and the repeat motifs from clinical samples in Saudi Arabia, it will contribute towards the rational design of antiadhesion drug therapies for P. falciparum malaria.
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