Fruits are vital portion of healthy diet owed to rich source of vitamins, minerals, and dietary fibers, which are highly favorable in keeping individual fit. Unfortunately, these days, one-third of ...fruits were infested with fungi and their toxic metabolites called mycotoxins, which is most annoying and pose significant health risk. Therefore, there is a need to suggest appropriate mitigation strategies to overcome the mycotoxins contamination in fruits. In the present study, detoxification efficiency of irradiation on zearalenone (ZEA) mycotoxin was investigated in distilled water and fruit juices (orange, pineapple, and tomato) applying statistical program response surface methodology (RSM). The independent factors were distinct doses of irradiation and ZEA, and response factor was a percentage of ZEA reduction in content. A central composite design (CCD) consists of 13 experiments were planned applying software program Design expert with distinct doses of irradiation (up to 10 kGy) and ZEA (1-5 μg). The results revealed that independent factors had a positive significant effect on the response factor. The analysis of variance (ANOVA) was followed to fit a proper statistical model and suggested that quadratic model was appropriate. The optimized model concluded that doses of irradiation and ZEA were the determinant factors for detoxification of ZEA in fruit juices. Further, toxicological safety of irradiation mediated detoxified ZEA was assessed in the cell line model by determining the cell viability (MTT and live/dead cell assays), intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), nuclear damage, and caspase-3 activity. The higher level of live cells and MMP, lower extent of intracellular ROS molecules and caspase-3, and intact nuclear material were noticed in cells treated with irradiation mediated detoxified ZEA related to non-detoxified ZEA. The results confirmed that toxicity of ZEA was decreased with irradiation treatment and detoxification of ZEA by irradiation is safe. The study concluded that irradiation could be a potential post-harvest food processing technique for detoxification of ZEA mycotoxin in fruit juices. However, irradiation of fruit juices with high dose of 10 kGy has minimally altered the quality of fruit juices.
Application of synthetic fungicides in agricultural commodities has been restricted due to development of fungicide resistance fungi and deleterious impact on environment and health of farm animals ...and humans. Hence, there is an urge for development of mycobiocides, and the present study was undertaken to determine the antifungal activity of
essential oil (CMEO) on post-harvest pathogen
. The CMEO was extracted by hydrodistillation and GC-MS chemical profile revealed the presence of 46 compounds and abundant was geraniol (19.06%). The minimum inhibitory concentration and minimum fungicidal concentration of CMEO were determined as 421.7 ± 27.14 and 618.3 ± 79.35 ppm, respectively. The scanning electron microscopic observation of CMEO exposed macroconidia was exhibited a detrimental morphology with vesicles, craters, protuberance, and rough surfaces related to control fungi. The CMEO induced the death of fungi through elevating intracellular reactive oxygen species and lipid peroxidation, and depleting ergosterol content. Regrettably, essential oils are highly volatile and become unstable and lose their biological features on exposure to light, heat, pH, moisture, and oxygen. To overcome these issues, chitosan encapsulated CMEO nanoparticles (Ce-CMEO-NPs) were prepared. The synthesized Ce-CMEO-NPs have spherical morphology with Zeta potential of 39.3-37.2 mV and their corresponding size was found in range of 455-480 nm. The Fourier transform infrared analysis confirmed that bio-active constituents of CMEO were well stabilized due to chitosan conjugation and successfully formed Ce-CMEO-NPs. The
release assay observed that the release of CMEO is stabilized due to the complex formation with chitosan and thereby, increases the lifetime antifungal activity of CMEO by gradual release of antifungal constituents of Ce-CMEO-NPs. In conclusion, antifungal and antimycotoxin activities of CMEO and Ce-CMEO-NPs against
were assessed in maize grains under laboratory conditions over a storage period of 28 days. Interestingly, Ce-CMEO-NPs were presented efficient and enhanced antifungal and antimycotoxin activities related to CMEO, and it could be due to perseverance of antifungal activity by controlled release of antifungal constituents from Ce-CMEO-NPs. The study concluded that Ce-CMEO-NPs could be highly appropriate as mycobiocides in safeguarding the agricultural commodities during storage period in agricultural and food industries.
Downy mildew of pearl millet caused by the biotrophic oomycete Sclerospora graminicola is the most devastating disease which impairs pearl millet production causing huge yield and monetary losses. ...Chitosan nanoparticles (CNP) were synthesized from low molecular weight chitosan having higher degree of acetylation was evaluated for their efficacy against downy mildew disease of pearl millet caused by Sclerospora graminicola. Laboratory studies showed that CNP seed treatment significantly enhanced pearl millet seed germination percentage and seedling vigor compared to the control. Seed treatment with CNP induced systemic and durable resistance and showed significant downy mildew protection under greenhouse conditions in comparison to the untreated control. Seed treatment with CNP showed changes in gene expression profiles wherein expression of genes of phenylalanine ammonia lyase, peroxidase, polyphenoloxidase, catalase and superoxide dismutase were highly upregulated. CNP treatment resulted in earlier and higher expression of the pathogenesis related proteins PR1 and PR5. Downy mildew protective effect offered by CNP was found to be modulated by nitric oxide and treatment with CNP along with NO inhibitors cPTIO completely abolished the gene expression of defense enzymes and PR proteins. Further, comparative analysis of CNP with Chitosan revealed that the very small dosage of CNP performed at par with recommended dose of Chitosan for downy mildew management.
The present study was aimed to determine the inhibitory effects of Curcuma longa L. essential oil (CLEO) against the growth and zearalenone (ZEA) production of Fusarium graminearum. Chemical ...profiling of CLEO was carried out by GC-FID analysis and major compound was ar-turmerone (53.10%). Antifungal activity of CLEO was assessed by using micro-well dilution method, and minimum inhibitory (MIC) and minimum fungicidal concentrations (MFC) were determined as 2450 and 3300 μg/mL, respectively. Interestingly, ROS played a major role in antifungal activity as evidenced by reactive oxygen species estimation from CLEO treated fungal cultures. Further, Scanning electron microscopy observations were carried at MIC and MFC concentrations. Results indicated that, CLEO significantly affected the morphology of mycelia and spore structures compared to untreated control culture. Effects of CLEO on fungal biomass as well as ZEA production were assessed by dry-weight and UHPLC analysis, respectively. Results indicated that, fungal biomass and zearalenone production was completely inhibited at 3500 and 3000 μg/mL, respectively. The results of the study conclude that, CLEO may find a potential application in the food industry to control the fungal infestation and ZEA contamination.
•GC–MS analysis of Curcuma longa essential oil (CLEO) revealed presence of 35 compounds.•ABTS and DPPH antioxidant activities of CLEO were 48.35 and 33.81 μg/mL.•MIC and MFC values of CLEO on Fusarium graminearum were determined as 2450 and 3300 μg/mL.•CLEO inhibited fungal biomass and zearalenone production at 3500 and 3000 μg/mL.•CLEO showed antifungal activity through ROS induced apoptotic cell death.
Cancer is a major cause of death worldwide, with an increasing number of cases being reported annually. The elevated rate of mortality necessitates a global challenge to explore newer sources of ...anticancer drugs. Recent advancements in cancer treatment involve the discovery and development of new and improved chemotherapeutics derived from natural or synthetic sources. Natural sources offer the potential of finding new structural classes with unique bioactivities for cancer therapy. Endophytic fungi represent a rich source of bioactive metabolites that can be manipulated to produce desirable novel analogs for chemotherapy. This review offers a current and integrative account of clinically used anticancer drugs such as taxol, podophyllotoxin, camptothecin, and vinca alkaloids in terms of their mechanism of action, isolation from endophytic fungi and their characterization, yield obtained, and fungal strain improvement strategies. It also covers recent literature on endophytic fungal metabolites from terrestrial, mangrove, and marine sources as potential anticancer agents and emphasizes the findings for cytotoxic bioactive compounds tested against specific cancer cell lines.
In the present study, activated carbon (AC) was derived from seed shells of
and applied to decontaminate the zearalenone (ZEA) mycotoxin. The AC of
(ACJC) was prepared by ZnCl
chemical activation ...method and its crystalline structure was determined by X-ray diffraction analysis. The crystalline graphitic nature of ACJC was confirmed from the Raman spectroscopy. Scanning electron microscope showed the porous surface morphology of the ACJC surface with high pore density and presence of elemental carbon was identified from the energy dispersive X-ray analysis. From Brunauer-Emmett-Teller (BET) analysis, S
, micropore area, and average pore diameter of ACJC were calculated as 822.78 (m
/g), 255.36 (m
/g), and 8.5980 (Å), respectively. The adsorption of ZEA by ACJC was accomplished with varying contact time, concentration of ZEA and ACJC, and pH of media. The ACJC has adsorbed the ZEA over a short period of time and adsorption of ZEA was dependent on the dose of ACJC. The effect of different pH on adsorption of ZEA by ACJC was not much effective. Desorption studies confirmed that adsorption of ZEA by ACJC was stable. The adsorption isotherm of ZEA by ACJC was well fitted with Langmuir model rather than Freundlich and concluded the homogeneous process of sorption. The maximum adsorption of ZEA by ACJC was detected as 23.14 μg/mg. Finally, adsorption property of ACJC was utilized to establish ACJC as an antidote against ZEA-induced toxicity under
in neuro-2a cells. The percentage of live cells was high in cells treated together with a combination of ZEA and ACJC compared to ZEA treated cells. In a similar way, ΔΨ
was not dropped in cells exposed to combination of ACJC and ZEA compared to ZEA treated cells. Furthermore, cells treated with a combination of ZEA and ACJC exhibited lower level of intracellular reactive oxygen species and caspase-3 compared to ZEA treated cells. These
studies concluded that ACJC has successfully protected the cells from ZEA-induced toxicity by lowering the availability of ZEA in media as a result of adsorption of ZEA. The study concluded that ACJC was a potent decontaminating agent for ZEA and could be used as an antidote against ZEA-induced toxicity.
is a leading plant pathogen that causes Fusarium head blight, stalk rot, and Gibberella ear rot diseases in cereals and posing the immense threat to the microbiological safety of the food. Herein, we ...report the green synthesis of zinc oxide nanoparticles from
(SaZnO NPs) flower bud extract by combustion method and investigated their application for controlling of growth and mycotoxins of
. Formation of SaZnO NPs was confirmed by spectroscopic methods. The electron microscopic (SEM and TEM) analysis revealed the formation of triangular and hexagonal shaped SaZnO NPs with size range 30-40 nm. The synthesized SaZnO NPs reduced the growth and production of deoxynivalenol and zearalenone of
in broth culture. Further analysis revealed that treatment of mycelia with SaZnO NPs resulted in the accumulation of ROS in the dose-dependent manner. Also, SaZnO NPs treatment enhanced lipid peroxidation, depleted ergosterol content, and caused detrimental damage to the membrane integrity of fungi. Moreover, SEM observations revealed that the presence of diverged micro-morphology (wrinkled, rough and shrank surface) in the macroconidia treated with SaZnO NPs. Taken together, SaZnO NPs may find a potential application in agriculture and food industries due to their potent antifungal activity.
Deoxynivalenol (DON) is Fusarium mycotoxin that is frequently found in many cereal-based foods, and its ingestion has a deleterious impact on human health. In this investigation, we studied the ...mechanism of DON-induced neurotoxicity and followed by cytoprotective efficacy of quercetin (QUE) in contradiction of DON-induced neurotoxicity through assessing the oxidative stress and apoptotic demise in the human neuronal model, i.e. SH-SY5Y cells. DON diminished the proliferation of cells in the manner of dose and time-dependent as revealed by cell viability investigations, i.e. MTT and lactate dehydrogenase assays. Additional studies, such as intracellular reactive oxygen species (ROS), lipid peroxidation (LPO), mitochondrial membrane potential (MMP), DNA damage, cell cycle, and neuronal biomarkers (amino acid decarboxylase, tyrosine hydroxylase, and brain-derived neurotrophic factor) demonstrated that DON induces apoptotic demise in neuronal cells through oxidative stress intermediaries. On another hand, pre-treatment of neuronal cells with 1 mM of quercetin (QUE) showed decent viability upon exposure to 100 µM of DON. In detailed studies demonstrated that QUE (1 mM) pre-treated cells show strong attenuation efficiency against DON-induced ROS generation, LPO, MMP loss, DNA impairment, cell cycle arrest, and down-regulation of neuronal biomarkers. The consequences of the investigation concluded that QUE mitigates the DON-induced stress viz., decreased ROS production and LPO generation, upholding MMP and DNA integrity and regulation of neuronal biomarker gene expression in SH-SY5Y cells.
Resistant Starch (RS), plays a crucial role in human health and nutrition by controlling glucose metabolism. RS or dietary fibre content in rice is low because it goes through a variety of process ...before it is ready for cooking and consumption. Hence, this study was carried out to develop a rice mutant with increased RS. The rice mutant (γ278) with increased RS was developed by utilizing gamma (γ) rays as a mutagen. Mutant γ278 was characterized for mutations in the starch biosynthetic genes
viz
.,
GBSSI, SSI, SSIIa, SSIIIa, SBEIa
, and
SBEIIb
to reveal the functional mutations/variations led to high RS content in rice. A total of 31 sequence variants/mutations in six genes were identified. We report the discovery of three deleterious mutation/variants each in
GBSSI, SSIIa
, and
SSIIIa
with the potential to increase RS content in rice. Further,
wild
×
mutant
crosses were made to develop an F
2
population to study the effect of combination of deleterious mutations. The SNP (
GBSSI
:
ssIIa
:
ssIIIa
) combination responsible for high RS content in F
2
population was identified and recorded highest amylose content (AC) (26.18%) and RS (8.68%) content. In conclusion, this marker combination will be highly useful to develop a rice variety with increased RS.
Nowadays, contamination of agricultural commodities with fungi and their mycotoxins is one of the most annoying with regard to food safety and pose serious health risk. Therefore, there is a ...requisite to propose suitable mitigation strategies to reduce the contamination of fungi and mycotoxins in agricultural commodities. In the present study, combinational inhibitory effect of
L. essential oil (HSEO) and radiation was established on growth rate, production of deoxynivalenol (DON) and zearalenone (ZEA) by
in maize grains. The HSEO was obtained from rhizomes by hydrodistillation technique and chemical composition was revealed by GC-MS analysis. A total of 48 compounds were identified and major compounds were 1,8-cineole (23.15%), linalool (12.82%), and β-pinene (10.06%). The discrete treatments of HSEO and radiation were effective in reducing the fungal growth rate and mycotoxins content, and the complete reduction was noticed at 3.15 mg/g of HSEO and 6 kGy of radiation. Response surface methodology (RSM) was applied to evaluate the combinational inhibitory effect of HSEO and radiation treatments on fungal growth rate and mycotoxins content. A total of 13 experiments were designed with distinct doses of HSEO and radiation by central composite design (CCD) of Stat-Ease Design-Expert software. In combinational approach, complete reductions of fungal growth, DON, and ZEA content were noticed at 1.89 mg/g of HSEO and 4.12 kGy of radiation treatments. The optimized design concluded that combinational treatments of HSEO and radiation were much more effective in reducing the fungal growth and mycotoxins content compared to their discrete treatments (
< 0.05). Responses of the design were assessed by second-order polynomial regression analysis and found that quadratic model was well fitted. The optimized design has larger
-value and adequate precision, smaller
-value, decent regression coefficients (
) and found statistically significant (
< 0.05). In addition, correlation matrix, normal plot residuals, Box-Cox, and actual vs. predicted plots were endorsed that optimized design was accurate and appropriate. The proposed combinational decontamination technique could be highly applicable in agriculture and food industry to safeguard the food and feed products from fungi and mycotoxins.
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