Imiquimod (IMQ) induced human-like psoriasis in mice has been shown to be effective in testing and development of novel treatments. The IMQ psoriasis model has become widely used animal model, ...however, it is not completely characterized in different rat strains. We aimed to evaluate IMQ and betamethasone treatment for induction and reversal of psoriatic lesions on macroscopic, histological, genetic as well as cytokines and chemokines activation levels. Wistar rats were treated topically with IMQ. Adopted Psoriasis Area Severity Index (PASI) was calculated at the baseline, after the IMQ-symptoms induction and after betamethasone-symptoms reversal. Systematic effects were studied on cytokines and chemokines levels in plasma. Skin biopsy was taken to assess histological symptoms and selected inflammatory cytokines and receptors genes expression levels. Reversal of skin lesions, after betamethasone treatment, was significant (p = 0.03). Histological differences between untreated and IMQ-treated skin were significant for some markers (p < 0.05) though not significantly decreased by betamethasone treatment. Fourteen genes were significantly up-regulated after the IMQ and four genes were down-regulated after skin lesions reversal by betamethasone. This work provides new insights on biological effects of imiquimod induced psoriasis and its reversal by betamethasone treatment in Wistar rats. It also contributes to general knowledge of the rat model usage for testing of novel anti-psoriasis drugs.
is a globally significant parasitic nematode in ruminants, with widespread resistance to benzimidazole due to its excessive and prolonged use. Given the extensive use of benzimidazole anthelmintics ...in Bosnia and Herzegovina, we hypothesized that resistance is prevalent. The aim of this study was to identify the presence of anthelmintic resistance to benzimidazole in
from naturally infected sheep, goats and cattle in Bosnia and Herzegovina through the detection of the Phe/Tyr polymorphism in the amino acid at position 200 of the β-tubulin protein. From 19 locations in Bosnia and Herzegovina, a total of 83 adult
were collected from the abomasum of ruminants. Among these, 45
specimens were isolated from sheep, 19 from goats and 19 from cattle. Results showed that 77.8% of
in sheep exhibited homozygous resistant genotypes at position 200 of the β-tubulin gene, with 15.5% being heterozygous. In goats, all tested
(100%) were homozygous resistant, and no heterozygous resistant or homozygous sensitive genotypes were found. Cattle had 94.7% homozygous resistant
, with no heterozygous resistant genotypes detected. In
from sheep and cattle, 6.7% and 5.3%, respectively, displayed homozygous sensitive genotypes. This study, for the first time, highlights the presence of a resistant population of
in sheep, goats and cattle in Bosnia and Herzegovina, using the rt-qPCR method. The resistance likely spread from sheep or goats to cattle, facilitated by shared pastures and the practice of transhumance, indicating a widespread and growing issue of anthelmintic resistance.
Boron and boron containing compounds are known for their biological and protective roles being non-toxic and non-mutagenic in low concentrations. Male rats were exposed to halogenated boroxine (HB), ...dipotassium-trioxohydroxytetrafluorotriborate K2B3O3F4OH, a potential new boron-containing therapeutic, aiming to determine concentrations with no adverse effects on selected serum biochemical parameters and histomorphological features.
HB was prepared by reacting potassium hydrofluoride (KHF2) with boric acid in molar ratios 2:3 at room temperature and its primary structure contains 4 fluorine atoms substituted in 6-membered ring. In concentrations of 10, 25, 35 and 45 mg/kg, HB was administered intraperitoneally as a single dose. Biochemical parameters were observed 24 and 96 h following the treatment. Effects of HB on biochemical blood parameters were also observed 24 h following continuous nine days application in concentrations of 10 mg/kg intraperitoneally and 50 mg/kg per os. Histomorphological observation of kidneys, liver, spleen, lungs and heart was performed for all treated animals.
Administration of single high dose of HB (35 mg/kg–45 mg/kg) effected high levels of urea and creatinine, which indicated renal injury that appeared to be temporary. Possible cause of concern is pancreatic injury indicated by elevated levels of serum amylase in the groups of animals that received the highest dosages of the substance. Histopathological examination of selected tissues revealed mild to moderate lesions in the kidneys and livers associated with administration of HB.
Observation of biochemical serum parameters or histopathology of examined tissues revealed no adverse effects of HB either after the administration of single dose lower than 35 mg/kg or following repeated administration at 10 mg/kg. These dosages should be further considered for potential therapeutic applications.
Nowadays, in order to maintain cleanliness in our houses, as well as to maintain personal hygiene, numerous chemical agents are used every day. Dishwashing detergents are used the most, followed by ...laundry detergents, various soaps and hair sham?poos, and not infrequently descaling agents, for cleaning ovens, unclogging drainage and sewage pipes, neutralizing unpleasant odours, etc. Although their number is growing day by day, most people do not realize the consequences, or at least not enough, and especially not about their potentially toxic effects. In fact, a great deal of the population do not consider household chemicals a particular problem, which is true, if they are used in the pre?scribed manner. However, the fact that these agents can cause harmful effects in humans and animals, and even participate in environmental pollution, is more than a sufficient reason to pay more attention to these agents, that is, to talk about them more and obtain more information for that matter. Particularly since there are authors who believe that some of the mentioned agents can even cause cancer, asthma or birth defects, i.e. infertility. Household chemicals can be divided in several ways, and one of the classifications is the one made according to the place of use. According to this division, the products we use every day at home can be roughly divided into those used for cleaning and maintaining hygiene in kitch?ens, used for the same purposes in bathrooms (including those used for personal hygiene ), and the products used in rooms and on terraces (flower protection).
Antimicrobial agents in laying hens Cupic, Vitomir; Ivanovic, Sasa; Borozan, Suncica ...
Zbornik Matice Srpske Za Prirodne Nauke,
2022, Letnik:
2022, Številka:
142
Journal Article
Recenzirano
Odprti dostop
The European Union permitted 6 antimicrobial agents that can be used in
laying hens. These are colistin, tyrosine, neomycin, oxytetracycline,
chlortetracycline, and erythromycin. Antimicrobial drugs ...are used today
primarily for the prevention and treatment of diseases in poultry and often
(not in the EU) to stimulate growth. Because these drugs are often used
irrationally, there are good chances that their residues will be found not
only in poultry meat but also in the eggs within a certain period after the
termination of treatment. In addition to the administration of authorised
VMPs, the residues in eggs can be the result of erroneously applied
medicated food, the contamination of the food with some antimicrobial drug
in the mixing unit, as well as ?extra-label? use of drugs in poultry. The
antimicrobial agents are distributed in the body and deposited in the eggs,
mainly in the yolk where they persist longer than in the albumen. Drugs that
are poorly absorbed from the gastrointestinal tract (aminoglycosides,
aminocyclitols, polymyxins) cannot be detected in the eggs, while the
residues of some antimicrobial drugs can be detected for up to two months
(chloramphenicol) after the last treatment. The rational use of drugs in
veterinary medicine has manifold significance. When using drugs only when
they are really necessary (indicated), in the right dose and route of
administration, the potential damage can be reduced and efficiency
increased, while the risk of microorganism resistance development would be
significantly decreased. All of this becomes more important when these drugs
are used in food animals.
Haemonchus contortus is a globally significant parasitic nematode in ruminants, with widespread resistance to benzimidazole due to its excessive and prolonged use. Given the extensive use of ...benzimidazole anthelmintics in Bosnia and Herzegovina, we hypothesized that resistance is prevalent. The aim of this study was to identify the presence of anthelmintic resistance to benzimidazole in H. contortus from naturally infected sheep, goats and cattle in Bosnia and Herzegovina through the detection of the Phe/Tyr polymorphism in the amino acid at position 200 of the β-tubulin protein. From 19 locations in Bosnia and Herzegovina, a total of 83 adult H. contortus were collected from the abomasum of ruminants. Among these, 45 H. contortus specimens were isolated from sheep, 19 from goats and 19 from cattle. Results showed that 77.8% of H. contortus in sheep exhibited homozygous resistant genotypes at position 200 of the β-tubulin gene, with 15.5% being heterozygous. In goats, all tested H. contortus (100%) were homozygous resistant, and no heterozygous resistant or homozygous sensitive genotypes were found. Cattle had 94.7% homozygous resistant H. contortus, with no heterozygous resistant genotypes detected. In H. contortus from sheep and cattle, 6.7% and 5.3%, respectively, displayed homozygous sensitive genotypes. This study, for the first time, highlights the presence of a resistant population of H. contortus in sheep, goats and cattle in Bosnia and Herzegovina, using the rt-qPCR method. The resistance likely spread from sheep or goats to cattle, facilitated by shared pastures and the practice of transhumance, indicating a widespread and growing issue of anthelmintic resistance.
Nitrofurans' residues in food of animal origin Smajlović, A., Fakultet veterinarske medicine, Sarajevo (Bosnia and Herzegovina); Mujezinović, I., Fakultet veterinarske medicine, Sarajevo (Bosnia and Herzegovina); Ćupić, V., Fakultet veterinarske medicine, Beograd (Serbia) ...
Veterinarski glasnik,
(2011), Letnik:
65, Številka:
3-4
Journal Article
Recenzirano
Odprti dostop
Nitrofurans are synthetic broad-spectrum antimicrobial agents that are often used in commercial animal production because of their excellent antibacterial and pharmacokinetic properties. However, ...nitrofurans and their metabolites have been shown to have potentially carcinogenic and mutagenic characteristics which has led to a ban on the use of nitrofurans in preventive and therapeutic treatment of animals used for food production. Metabolites of nitrofurans that can be determined after their application are: a metabolite of furazolidone, furaltadone metabolite, a metabolite of nitrofurantoin and nitrofurazone metabolite. The presence of residues of nitrofuran antibiotics in meat, fish and shrimps, and milk and eggs originating from countries outside the European Union is monitored and recorded by the RASFF system of the European Union. Furthermore, since nitrofurans are used in some countries as prophylactic agents and growth promoters, it is necessary to carry out constant control of various types of food of animal origin, in order to reduce to the minimum potential carcinogenic and mutagenic effects of these supstances for the health of consumers. In Bosnia and Herzegovina, there is no permanent control of nitrofurans in food of animal origin. The provisions of the Regulation on the maximum allowable amounts of veterinary drugs and pesticides in products of animal origin, published in the Official Gazette of Bosnia and Herzegovina state the prohibiting of the use of certain veterinary drugs in animals intended for human consumption, including nitrofurans. The European Union has established the minimum required limit (MRLP) for performance which is 1 microg/kg of nitrofurans for edible tissues of animal origin. Taking all this into account, methods for nitrofurans detection should be accreditated and validated, both for screening and confirmatory methods, and further research into the presence of nitrofurans in food of animal origin in Bosnia and Herzegovina should be performed.
Serotonin or 5-hydroxytryptamine (5-HT), is a monoamine neurotransmitter synthesised from L-tryptophan in serotonergic neurons and enterochromaffin cells of the gastrointestinal tract. This ...neurotransmitter is widely distributed in the animal and plant kingdom and regulates some central and peripheral functions through several types of specific serotonergic (5-HT) receptors. Since it is known that the effect of serotonin, especially in pathological conditions, is very important, we believe that determining the types of receptors for this substance would make it possible to use their agonist or antagonists, which would undoubtedly enhance the pharmacotherapy of functional disruption of the small intestine in broilers. Investigations were carried out on isolated smooth muscle strips of the circular and longitudinal layer of the broiler small intestine (strip dimension 3-4 mm x 2 cm). The muscle strips were placed in an isolated organ bath. The mechanical activity of the preparations was recorded via an isotonic force transducer coupled to a pen recorder. This was done following the addition of serotonin (nonselective 5-HT agonist), 8-OH-DPAT (selective 5-HT1A agonist) and spiroxatrin (selective 5-HT1A antagonist). The sensitivity of the tissues to acetylcholine was tested before starting the experiments. Using the obtained results, it can be concluded that 5HT1A type receptors are present in smooth muscles of the broiler small intestine, duodenum and ileum, especially in the longitudinal smooth muscle layer which reacted with contractions even to low serotonin concentration (10-6), but not in the jejunum.
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