•Sesame straw biochar demonstrated an excellent adsorption capacity for heavy metals.•Adsorption fitted best with Langmuir model as compared to the Freundlich model.•High interference among the ...metals was observed under multimetal sorption condition.•The highest reduction in adsorption capacity was found for Cd.•Adsorption behaviors was well described by three-dimensional simulation.
Objective of this research was to evaluate adsorption of heavy metals in mono and multimetal forms onto sesame straw biochar (SSB). Competitive sorption of metals by SSB has never been reported previously. The maximum adsorption capacities (mgg−1) of metals by SSB were in the order of Pb (102)≫Cd (86)≫Cr (65)>Cu (55)≫Zn (34) in the monometal adsorption isotherm and Pb (88)≫Cu (40)≫Cr (21)>Zn (7)⩾Cd (5) in the multimetal adsorption isotherm. Based on data obtained from the distribution coefficients, Freundlich and Langmuir adsorption models, and three-dimensional simulation, multimetal adsorption behaviors differed from monometal adsorption due to competition. Especially, during multimetal adsorption, Cd was easily exchanged and substituted by other metals. Further competitive adsorption studies are necessary in order to accurately estimate the heavy metal adsorption capacity of biochar in natural environments.
Organic photovoltaics are an important part of a next‐generation energy‐harvesting technology that uses a practically infinite pollutant‐free energy source. They have the advantages of light weight, ...solution processability, cheap materials, low production cost, and deformability. However, to date, the moderate photovoltaic efficiencies and poor stabilities of organic photovoltaics impede their use as replacements for inorganic photovoltaics. Recent developments in bulk‐heterojunction organic photovoltaics mean that they have almost reached the lower efficiency limit for feasible commercialization. In this review article, the recent understanding of the ideal bulk‐heterojunction morphology of the photoactive layer for efficient exciton dissociation and charge transport is described, and recent attempts as well as early‐stage trials to realize this ideal morphology are discussed systematically from a morphological viewpoint. The various approaches to optimizing morphologies consisting of an interpenetrating bicontinuous network with appropriate domain sizes and mixed regions are categorized, and in each category, the recent trends in the morphology control on the multilength scale are highlighted and discussed in detail. This review article concludes by identifying the remaining challenges for the control of active layer morphologies and by providing perspectives toward real application and commercialization of organic photovoltaics.
The morphology of the bulk‐heterojunction photoactive layer critically influences charge‐carrier dynamics and therefore the overall photovoltaic performance of organic photovoltaics. The morphological issues reported so far are discussed and future directions toward further optimization are suggested.
Acinetobacter baumannii is an opportunistic pathogen that causes serious nosocomial infection in intensive care units. In particular, carbapenem-resistant A. baumannii (CRAB) strains have been ...increasing in the past decade, and they have caused major medical problems worldwide. In this study, a novel A. baumannii lytic phage, the YMC 13/03/R2096 ABA BP (phage Βϕ-R2096), which specifically causes the lysis of CRAB strains, was characterized in detail in vitro and in silico, and the in vivo effectiveness of phage therapy was evaluated using Galleria mellonella and a mouse model of acute pneumonia.
The A. baumannii phage Βϕ-R2096 was isolated from sewage water using CRAB clinical strains selected from patients at a university hospital in South Korea. The complete genome of the phage Βϕ-R2096, which belongs to the Myoviridae family, was analyzed. Phage Βϕ-R2096 inhibited bacterial growth in a dose-dependent manner and exhibited high bacteriolytic activity at MOI = 10. In the evaluation of its therapeutic potential against CRAB clinical isolates using two in vivo models, phage Βϕ-R2096 increased the survival rates of both G. mellonella larvae (from 0 to 50% at 24 h) and mice (from 30% with MOI = 0.1 to 100% with MOI = 10 for 12 days) in post-infection of CRAB. In particular, phage Βϕ-R2096 strongly ameliorated histologic damage to infected lungs, with bacterial clearance in the lungs observed on day 3 postinfection in the mouse acute pneumonia model. Moreover, in vivo studies revealed no mortality or serious side effects in phage-treated groups.
The results of this study strongly suggest that phage Βϕ-R2096, a novel A. baumannii lytic phage, could be an alternative antibacterial agent to control CRAB infections. This study is the first report to compare in vivo evaluations (G. mellonella larvae and a mouse acute pneumonia model) of the therapeutic efficacy of a phage against CRAB infections.
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This study investigated the characteristics of biochars derived using various pine tree residues and pyrolysis temperatures and evaluated their Cd adsorption behaviors. The ...characteristics of pine tree residue biochars (PRBs) were dominantly affected by the pyrolysis temperature, and the optimum pyrolysis temperature for Cd adsorption was 600 °C. The adsorption of Cd by PRBs was divided into two stages: rapid adsorption on the initial boundary layer and slow adsorption by intraparticle diffusion. The Cd adsorption characteristics of all the PRBs were well described by pseudo-second-order and Langmuir isotherm models, and the maximum adsorption capacity was the highest in pine bark biochar (85.8 mg/g). The amounts of the cations released from the mixed pine tree residue biochars (M-PRBs) during Cd adsorption were increased, while the amount of phosphate released was decreased, indicating that exchangeable cations and phosphate on the biochar affected the Cd adsorption. In particular, the amount of Cd removed by the exchangeable cations corresponds to 23.6% of the total adsorption amount. Spectroscopic analyses using FTIR showed that the Cd adsorption on M-PRB was associated with functional groups such as CC, COH and COOH. Overall, the use of biochars derived from pine tree residue as an adsorbent is considered to be effective for both the treatment of wastewater containing heavy metals and the recycling of forest residues.
Coronavirus disease 2019 (COVID-19) is a newly emerged human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In a global pandemic, development of a cheap, ...rapid, accurate, and easy-to-use diagnostic test is necessary if we are to mount an immediate response to this emerging threat. Here, we report the development of a specific lateral flow immunoassay (LFIA)-based biosensor for COVID-19. We used phage display technology to generate four SARS-CoV-2 nucleocapsid protein (NP)-specific single-chain variable fragment-crystallizable fragment (scFv-Fc) fusion antibodies. The scFv-Fc antibodies bind specifically and with high affinity to the SARS-CoV-2 NP antigen, but not to NPs of other coronaviruses. Using these scFv-Fc antibodies, we screened three diagnostic antibody pairs for use on a cellulose nanobead (CNB)-based LFIA platform. The detection limits of the best scFv-Fc antibody pair, 12H1 as the capture probe and 12H8 as the CNB-conjugated detection probe, were 2 ng antigen protein and 2.5 × 104 pfu cultured virus. This LFIA platform detected only SARS-CoV-2 NP, not NPs from MERS-CoV, SARS-CoV, or influenza H1N1. Thus, we have successfully developed a SARS-CoV-2 NP-specific rapid diagnostic test, which is expected to be a simple and rapid diagnostic test for COVID-19.
•SARS-CoV-2 NP-specific scFv-Fc antibodies were generated.•Antibody pairs for the specific and sensitive detection of SARS-CoV-2 were found.•Lateral flow immunoassay-based rapid diagnostic tests sensitively detected NP antigen and SARS-CoV-2 virus.•The COVID-19 biosensor showed no cross-reactivity with other coronaviruses, SARS-CoV and MERS-CoV.
Enhanced performance of an inverted‐type polymer solar cell is reported by controlling the surface energy of a zinc oxide (ZnO) buffer layer, on which a photoactive layer composed of a ...polymer:fullerene‐derivative bulk heterojunction is formed. With the approach based on a mixed self‐assembled monolayer, the surface energy of the ZnO buffer layer can be controlled between 40 mN m−1 and 70 mN m−1 with negligible changes in its work function. For the given range of surface energy the power conversion efficiency increases from 3.27% to 3.70% through enhanced photocurrents. The optimized morphology obtained by surface energy control results in the enhanced photocurrent and transmission electron microscopy analysis verifies the correlation between the surface energy and the phase morphology of the bulk heterojunction. These results demonstrate that surface energy control is an effective method for further improving the performance of polymer solar cells, with potentially important implications for other organic devices containing an interface between a blended organic active layer and a buffer or an electrode layer.
Enhanced power conversion efficiency in polymer solar cells is achieved by surface energy control. Surface modification based on a mixed self‐assembled monolayer allows the independent control of the surface energy and work function. These results demonstrate that controlling the surface energy of a buffer layer that lies underneath a photoactive layer is an effective method for improving the performance in polymer solar cells.
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•FSB achieved 99.7% of OG removal efficiency within 2 h in Fenton-like oxidation.•Optimum ratio of H2O2:FSB:OG (g/L) for Fenton-like oxidation was 0.75:5:1.•OG removal by FSB showed ...initial induction followed by fast decomposition.•FSB use in Fenton-like oxidation offers greater benefits of efficiency, low-cost and reusability.
This study was conducted to evaluate the catalytic activity of Fe-impregnated sugarcane biochar (FSB) for removing azo dye Orange G (OG) from solution under various Fenton-like oxidation conditions. The optimum molar Fe concentration for impregnation to achieve maximum catalytic activity of FSB in Fenton-like reaction with acceptable effluent Fe release was 0.25 M (163.4 Fe mg/g in FSB). High removal efficiency of 99.7% was achieved within 2 h of reaction at optimum conditions of 0.075 g/L H2O2, 0.5 g/L FSB for 0.1 g/L OG at initial pH 5.5 under 25 °C. For every 10 °C increase, the time for maximum OG degradation efficiency decreased by 0.5 h. The OG removal by FSB exhibited a slow induction reaction followed by fast OG decomposition. FSB can be used successively for at least 4 runs with >89.3% OG removal. The FSB was more economical, efficient, and recyclable than other conventional Fenton oxidation catalysts.
Biochar has been used to remove heavy metals from aqueous solutions. In this study, a sulfurized wood biochar (SWB) by direct impregnation with elemental sulfur was produced and evaluated along with ...pristine wood biochar (WB) for adsorption characteristics and mechanism of mercury. Mercury adsorption by WB and SWB was well described by Langmuir model and pseudo second order model and the maximum adsorption capacities of WB and SWB were 57.8 and 107.5 mg g−1, respectively. Intraparticle diffusion model showed that mercury adsorption was fast due to boundary layer and slow adsorption due to diffusion into biochar pores. Although, mercury adsorption by both WB and SWB was predominantly influenced by the pH, temperature, salt concentration, and biochar dosage, the SWB showed a relatively stable mercury adsorption compared to WB under different conditions, suggesting the strong affinity of SWB for mercury. The XPS analysis showed different adsorption mechanisms of mercury between WB and SWB. In particular, mercury adsorption in WB was due to Hg-Cπ bond formation and interaction with carboxyl and hydroxyl groups, whereas in SWB it is primarily due to mercury interaction with C-SOx-C and thiophenic groups in addition to Hg-Cπ bond formation and interaction with carboxyl groups. The SEM-EDS mapping also demonstrated that mercury in SWB was related to carbon, oxygen and sulfur. Overall, the sulfurized biochar was effective for removing mercury from aqueous solution, and its direct production through pyrolysis with elemental sulfur impregnation of wood chips could make it an economic option as absorbent for treating mercury-rich wastewater.
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•A sulfurized biochar (SWB) was produced by pyrolysis of wood chip with elemental S.•SWB was an effective adsorbent for removing Hg than pristine biochar (WB).•Hg adsorbed by SWB was more stable and less susceptible to environment changes.•Hg adsorption by WB was dominated by interactions with C=C, COH and COO.•Hg by SWB involved thiophenic and C-SOx-C forms in addition to C=C and COO.
Helicobacter pylori colonization of the stomach affects about half of the world population and is associated with the development of gastritis, ulcers, and cancer. Polymorphisms in the IL1B gene are ...linked to an increased risk of H. pylori associated cancer, but the bacterial and host factors that regulate interleukin (IL)‐1β production in response to H. pylori infection remain unknown. Using murine BM‐derived DCs, we show that the bacterial virulence factors cytotoxin‐associated genes pathogenicity island and CagL, but not vacuolating cytotoxin A or CagA, regulate the induction of pro‐IL‐1β and the production of mature IL‐1β in response to H. pylori infection. We further show that the host receptors, Toll‐like receptor 2 (TLR2) and nucleotide‐binding oligomerization domain 2 (NOD2), but not NOD1, are required for induction of pro‐IL‐1β and NOD‐like receptor pyrin domain containing 3 (NLRP3) in H. pylori infected DCs. In contrast, NLRP3 and the adaptor ASC were essential for the activation of caspase‐1, processing of pro‐IL‐1β into IL‐1β, and IL‐1β secretion. Finally, we show that mice deficient in caspase‐1, IL‐1β, and IL‐1 receptor, but not NLRP3, are impaired in the clearance of CagA‐positive H. pylori from the stomach when compared with WT mice. These studies identify bacterial cag pathogenicity island and the cooperative interaction among host innate receptors TLR2, NOD2, and NLRP3 as important regulators of IL‐1β production in H. pylori infected DCs.
Many studies have focused on global hypomethylation or hypermethylation of tumor suppressor genes, but less is known about the impact of promoter hypomethylation of oncogenes. We previously showed ...that promoter methylation may gradually increase or decrease during the transition from gastric mucosa (GM) to intestinal metaplasia (IM) to gastric cancer (GC). In our study, we focused on regional CpG hypomethylation of the promoter‐proximal DNA of the transcription factor ONECUT2 (OC2) in IM and GC cells. We validated the hypomethylation of promoter‐proximal DNA of OC2 in 160 primary GCs, in which methylation level correlated negatively with OC2 mRNA level. IM and GC cells stained positively for OC2, whereas GM cells did not. Stable transfection of OC2 in GC cells promoted colony formation, cell migration, invasion and proliferation. Moreover, OC2 knockdown with a short hairpin RNA suppressed tumorigenesis in nude mice. In addition, chromatin immunoprecipitation coupled with DNA sequencing and RNA‐seq analyses revealed that OC2 triggered ACSL5, which is strongly expressed in IM of the stomach but not in GM, indicating that OC2 and ACSL5 are early‐stage biomarkers for GC. We also observed a high correlation between the levels of OC2 and ACSL5 mRNAs in the GENT database These results suggest that epigenetic alteration of OC2 upregulates its expression, which then activates ACSL5; thus, OC2 is induced in IM by epigenetic alteration and triggers ACSL5 expression, and thus OC2 and ACSL5 may cooperatively promote intestinal differentiation and GC progression.
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DNA hypomethylation can promote cancer development through activation of genes with oncogenic potential. Here, the authors found that CpGs in the promoter‐proximal DNA of ONECUT2 were hypomethylated in intestinal metaplasia and gastric cancers, and that hypomethylation was associated with ONECUT2 upregulation. Functional analysis demonstrated that ONECUT2 has oncogenic potential and could activate ACSL5, which is also expressed in intestinal metaplasia, suggesting that ONECUT2 and ACSL5 may cooperate to promote intestinal differentiation or development of gastric cancer. Taken together, the findings suggest that ONECUT2 and its downstream target ACSL5 could be used to develop early detection biomarkers and prevent gastric carcinogenesis.