Abstract
The gut microbiome is an important determinant in various diseases. Here we perform a cross-sectional study of Japanese adults and identify the
Blautia
genus, especially
B. wexlerae
, as a ...commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus. Oral administration of
B. wexlerae
to mice induce metabolic changes and anti-inflammatory effects that decrease both high-fat diet–induced obesity and diabetes. The beneficial effects of
B. wexlerae
are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and
l
-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition. These findings reveal unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders.
To increase the accuracy of microbiome data analysis, solving the technical limitations of the existing sequencing machines is required. Quality trimming is suggested to reduce the effect of the ...progressive decrease in sequencing quality with the increased length of the sequenced library. In this study, we examined the effect of the trimming thresholds (0-20 for QIIME1 and 0-30 for QIIME2) on the number of reads that remained after the quality control and chimera removal (the good reads). We also examined the distance of the analysis results to the gold standard using simulated samples.
Quality trimming increased the number of good reads and abundance measurement accuracy in Illumina paired-end reads of the V3-V4 hypervariable region.
Our results suggest that the pre-analysis trimming step should be included before the application of QIIME1 or QIIME2.
Atrial fibrillation (AF) reduces the quality of life by triggering stroke and heart failure. The association between AF onset and gut metabolites suggests a causal relationship between AF and gut ...microbiota dysbiosis; however, the relationship remains poorly understood. We prospectively enrolled 34 hospitalized patients with AF and 66 age-, sex-, and comorbidity-matched control subjects without a history of AF. Gut microbial compositions were evaluated by amplicon sequencing targeting the 16S ribosomal RNA gene. We assessed differences in dietary habits by using a brief-type self-administered diet history questionnaire (BDHQ). Gut microbial richness was lower in AF patients, although the diversity of gut microbiota did not differ between the two groups. At the genus level,
Enterobacter
was depleted, while
Parabacteroides
,
Lachnoclostridium
,
Streptococcus,
and
Alistipes
were enriched in AF patients compared to control subjects. The BDHQ revealed that the intake of n-3 polyunsaturated fatty acids and eicosadienoic acid was higher in AF patients. Our results suggested that AF patients had altered gut microbial composition in connection with dietary habits.
Memory isolation is an essential technology for safeguarding the resources of lightweight embedded systems. This technique isolates system resources by constraining the scope of the processor's ...accessible memory into distinct units known as domains. Despite the security offered by this approach, the Memory Protection Unit (MPU), the most common memory isolation method provided in most lightweight systems, incurs overheads during domain switching due to the privilege level intervention. However, as IoT environments become increasingly interconnected and more resources become required for protection, the significant overhead associated with domain switching under this constraint is expected to be crucial, making it harder to operate with more granular domains. To mitigate these issues, we propose DEMIX, which supports efficient memory isolation for multiple domains. DEMIX comprises two mainelements-
and
-with the primary idea of enabling granular access control for memory by validating the domain state of the processor and the executed instructions. By achieving fine-grained validation of memory regions, our technique safely extends the supported domain capabilities of existing technologies while eliminating the overhead associated with switching between domains. Our implementation of eight user domains shows that our approach yields a hardware overhead of a slight 8% in Ibex Core, a very lightweight RISC-V processor.
Inter-individual variations in gut microbiota composition are observed even among healthy populations. The gut microbiota may exhibit a unique composition depending on the country of origin and race ...of individuals. To comprehensively understand the link between healthy gut microbiota and host state, it is beneficial to conduct large-scale cohort studies. The aim of the present study was to elucidate the integrated and non-redundant factors associated with gut microbiota composition within the Japanese population by 16S rRNA sequencing of fecal samples and questionnaire-based covariate analysis.
A total of 1596 healthy Japanese individuals participated in this study via two independent cohorts, NIBIOHN cohort (n = 954) and MORINAGA cohort (n = 642). Gut microbiota composition was described and the interaction of these microorganisms with metadata parameters such as anthropometric measurements, bowel habits, medical history, and lifestyle were obtained. Thirteen genera, including Alistipes, Anaerostipes, Bacteroides, Bifidobacterium, Blautia, Eubacterium halli group, Faecalibacterium, Fusicatenibacter, Lachnoclostridium, Parabacteroides, Prevotella_9, Roseburia, and Subdoligranulum were predominant among the two cohorts. On the basis of univariate analysis for overall microbiome variation, 18 matching variables exhibited significant association in both cohorts. A stepwise redundancy analysis revealed that there were four common covariates, Bristol Stool Scale (BSS) scores, gender, age, and defecation frequency, displaying non-redundant association with gut microbial variance.
We conducted a comprehensive analysis of gut microbiota in healthy Japanese individuals, based on two independent cohorts, and obtained reliable evidence that questionnaire-based covariates such as frequency of bowel movement and specific dietary habit affects the microbial composition of the gut. To our knowledge, this was the first study to investigate integrated and non-redundant factors associated with gut microbiota among Japanese populations.
In this paper, we present a high-performance architecture for elliptic curve cryptography (ECC) over Curve448, which to the best of our knowledge, is the fastest implementation of ECC point ...multiplication over Curve448 to date. Firstly, we introduce a novel variant of the Karatsuba formula for asymmetric digit multiplier, suitable for typical DSP primitive with asymmetric input. It reduces the number of required DSPs compared to previous work and preserves the performance via full parallelization and pipelining. We then construct a 224-bit pipelined multiplier and interleaved fast reduction algorithm, yielding a total of 12 stages of pipelined modular multiplication with four stages of input delay. Additionally, we present an efficient Montgomery ladder scheduling with no additional register is required. The implementation on the Xilinx 7-series FPGA: Virtex-7, Kintex-7, Artix-7, and Zynq 7020 yields execution times of 0.12, 0.13, 0.24, and 0.24 ms, respectively. It increases the throughput by 242% compared to the best previous work on Zynq 7020 and by 858% compared to the best previous work on Virtex-7. Furthermore, the proposed architecture optimizes nearly 63% efficiency improvement in terms of Area <inline-formula> <tex-math notation="LaTeX">\times </tex-math></inline-formula> Time tradeoff. Lastly, we extend our architecture with well-known side-channel protections such as scalar blinding, base-point randomization, and continuous randomization.
It is important to determine the cause of increasing oral cancer occurrence and mortality rates in Japan, because the mortality rate has recently decreased in other developed countries. The impact of ...microbiota in carcinogenesis, especially in the digestive tract has been reported. This study aimed to clarify the relationship between oral cancer and oral microbiota in Japanese patients.
DNA was extracted from salivary samples of 60 oral cancer patients and 80 non-cancer individuals as controls. We performed metagenomic analysis using 16S rRNA amplicon sequencing. Statistical analysis in this study was performed using R (version 3.5.0).
Oral cancer patients showed higher α-diversity compared to the control group, and the β-diversity between the two groups differed significantly. Further, there was a significant difference in the abundance ratio of bacterial genera between the two groups. Peptostreptococcus, Fusobacterium, Alloprevotella, and Capnocytophaga were more abundant in the cancer group compared to the control, whereas Rothia and Haemophilus were less abundant (p < 0.01). A negative correlation in the microbiota composition was confirmed between the operational taxonomic units (OTU) of genus Rothia and T-stage progression using the TNM classification method. We performed logistic regression analysis to investigate the impact factor for the oral cancer group, and the result showed that Chao 1 index and sex are statistically significant variables.
In this study, we observed an increased bacterial diversity in oral cancer patients and found distribution changes for some bacteria.
With an ever-increasing interest in understanding the relationships between the microbiota and the host, more tools to map, analyze and interpret these relationships have been developed. Most of ...these tools, however, focus on taxonomic profiling and comparative analysis among groups, with very few analytical tools designed to correlate microbiota and the host phenotypic data. We have developed a software program for creating a web-based integrative database and analysis platform called MANTA (Microbiota And pheNoType correlation Analysis platform). In addition to storing the data, MANTA is equipped with an intuitive user interface that can be used to correlate the microbial composition with phenotypic parameters. Using a case study, we demonstrated that MANTA was able to quickly identify the significant correlations between microbial abundances and phenotypes that are supported by previous studies. Moreover, MANTA enabled the users to quick access locally stored data that can help interpret microbiota-phenotype relations. MANTA is available at https://mizuguchilab.org/manta/ for download and the source code can be found at https://github.com/chenyian-nibio/manta.
The pathogenesis of pulmonary arterial hypertension is closely associated with dysregulated inflammation. Recently, abnormal alterations in gut microbiome composition and function were reported in a ...pulmonary arterial hypertension experimental animal model. However, it remains unclear whether these alterations are a result or the cause of pulmonary arterial hypertension. The purpose of this study was to investigate whether alterations in the gut microbiome affected the hemodynamics in SU5416/hypoxia rats. We used the SU5416/hypoxia rat model in our study. SU5416/hypoxia rats were treated with a single SU5416 injection (30 mg/kg) and a three-week hypoxia exposure (10% O2). Three SU5416/hypoxia rats were treated with a combination of four antibiotics (SU5416/hypoxia + ABx group) for four weeks. Another group was exposed to hypoxia (10% O2) without the SU5416 treatment, and control rats received no treatment. Fecal samples were collected from each animal, and the gut microbiota composition was analyzed by 16S rRNA sequencing. The antibiotic treatment significantly suppressed the vascular remodeling, right ventricular hypertrophy, and increase in the right ventricular systolic pressure in SU5416/hypoxia rats. 16S rRNA sequencing analysis revealed gut microbiota modification in SU5416/hypoxia + ABx group. The Firmicutes-to-Bacteroidetes ratio in SU5416/hypoxia rats was significantly higher than that in control and hypoxia rats. Compared with the control microbiota, 14 bacterial genera, including Bacteroides and Akkermansia, increased, whereas seven bacteria, including Rothia and Prevotellaceae, decreased in abundance in SU5416/hypoxia rats. Antibiotic-induced modification of the gut microbiota suppresses the development of pulmonary arterial hypertension. Dysbiosis may play a causal role in the development and progression of pulmonary arterial hypertension.
Works on quantum computing and cryptanalysis have increased significantly in the past few years. Various constructions of quantum arithmetic circuits as one of the primary elements in the field have ...also been proposed. However, there have only been a few studies on finite field inversion despite its essential use in realizing quantum algorithms, such as in Shor's algorithm for Elliptic Curve Discrete Logarithm Problem (ECDLP). In this study, we propose to reduce the depth of the existing quantum Fermat's Little Theorem (FLT)-based inversion circuit for the binary finite field. In particular, we propose to follow a complete waterfall approach to translate the Itoh-Tsujii's variant of FLT to the corresponding quantum circuit and remove the inverse squaring operations employed in the previous work by Banegas et al., lowering the number of CNOT gates (i.e., CNOT count) as well as slightly reducing the <inline-formula> <tex-math notation="LaTeX">T </tex-math></inline-formula> depth, which contributes to a reduced overall depth and gate count. Furthermore, we concretely verify our method and compare it with the previous work in Qiskit, a quantum computer simulation environment, by constructing both our method and the previous work from scratch and performing the resource analysis. Additionally, we propose employing the relative-phase Toffoli gate by Gidney as opposed to the standard Toffoli implementation, which yields a significantly lower <inline-formula> <tex-math notation="LaTeX">T </tex-math></inline-formula> depth while further reducing the overall depth. Our approach can serve as an alternative for a time-efficient implementation.
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