This study examines the relationship between house museums and ICTs by looking at well-known examples which demonstrate that the use of ICTs in this context is not generalised. The benefits derived ...from the implementation of ICTs prove that this implementation is necessary to allow house museums to take a step forward and integrate data analysis into their communication plans with the objective of placing museum visitors at the centre of museum services. House museums will thereby be able to optimise their decision-making and adopt strategies that will improve the quality of visitors' museum experiences. Obtaining and analysing data about museum visitors and their experiences is made easier with Data technology, which can forecast patterns that illustrate types of visitor behaviour, helping museums to better understand and more fully profile visitors. In an increasingly digitalised market, house museums must generate interaction and interest to improve visitors' museum experiences.
Summary
The circadian clock of Arabidopsis thaliana controls many physiological and molecular processes, allowing plants to anticipate daily changes in their environment. However, developing a ...detailed understanding of how oscillations in mRNA levels are connected to oscillations in co/post‐transcriptional processes, such as splicing, has remained a challenge. Here we applied a combined approach using deep transcriptome sequencing and bioinformatics tools to identify novel circadian‐regulated genes and splicing events. Using a stringent approach, we identified 300 intron retention, eight exon skipping, 79 alternative 3′ splice site usage, 48 alternative 5′ splice site usage, and 350 multiple (more than one event type) annotated events under circadian regulation. We also found seven and 721 novel alternative exonic and intronic events. Depletion of the circadian‐regulated splicing factor AtSPF30 homologue resulted in the disruption of a subset of clock‐controlled splicing events. Altogether, our global circadian RNA‐seq coupled with an in silico, event‐centred, splicing analysis tool offers a new approach for studying the interplay between the circadian clock and the splicing machinery at a global scale. The identification of many circadian‐regulated splicing events broadens our current understanding of the level of control that the circadian clock has over this co/post‐transcriptional regulatory layer.
Significance Statement
In this work, we performed a global circadian transcriptomic study on alternative splicing in Arabidopsis thaliana; the first done on a member of the plant kingdom. Using a stringent approach with an in‐house‐developed bioinformatics tool, we identify novel clock‐controlled genes and 1513 splicing events that are under circadian regulation. Furthermore, we show that disruption of a circadian‐regulated splicing factor (AT2G02570/AtSPF30) affects a specific subset of the circadian‐controlled splicing events.
An important factor affecting acquisition of pollution elements could be the lichen growth form. The Brunauer–Emmett–Teller theory approach has been used to determinate the specific area surface ...(BET-area) of solids by gas multilayer adsorption. Taking this standard method as a new tool, we measure the specific thallus area in foliose and fruticose lichens to evaluated area/volume relation for bioaccumulation prospects. Some preliminary results of elemental contents such as REEs (La, Sc, Sr) and pollutants (Cd, Co, Pb) were also measured to support the importance to use for the analysis of these thallus attributes.
The mechanisms by which poikilothermic organisms ensure that biological processes are robust to temperature changes are largely unknown. Temperature compensation, the ability of circadian rhythms to ...maintain a relatively constant period over the broad range of temperatures resulting from seasonal fluctuations in environmental conditions, is a defining property of circadian networks. Temperature affects the alternative splicing (AS) of several clock genes in fungi, plants, and flies, but the splicing factors that modulate these effects to ensure clock accuracy throughout the year remain to be identified. Here we show that GEMIN2, a spliceosomal small nuclear ribonucleoprotein assembly factor conserved from yeast to humans, modulates low temperature effects on a large subset of pre-mRNA splicing events. In particular, GEMIN2 controls the AS of several clock genes and attenuates the effects of temperature on the circadian period in Arabidopsis thaliana . We conclude that GEMIN2 is a key component of a posttranscriptional regulatory mechanism that ensures the appropriate acclimation of plants to daily and seasonal changes in temperature conditions.
RNA processing, an important step in the regulation of gene expression, is mediated by proteins and RNA molecules that are highly sensitive to variations in temperature conditions. Most organisms do not control their own body temperature. Therefore, molecular mechanisms must have evolved that ensure that biological processes are robust to temperature changes. Here we identify a protein that buffers the effect of temperature on biological timing by enhancing the assembly of the spliceosome, a large ribonucleoprotein complex involved in RNA processing in organisms ranging from yeast to humans, and thereby controlling the alternative splicing of clock genes.
Red flags are increasingly being raised over the contribution of short term tourism rentals (mediated by platforms such as Airbnb) to socio-spatial inequalities and residential displacement. In many ...cases, the most vocal reactions have come from social movements claiming the Right to the City through particular geographies of resistance that underpin protest counter-narratives in the digital and non-digital spheres. In order to evaluate this relationship, a digital content analysis based on a sample of around 16,000 tweets illustrates the depth and diversity of tourism counter-narratives within the Twitter activity of social movements in opposition to tourism saturation in the city of Barcelona. This approach is triangulated with a spatial analysis of Airbnb-mediated rentals in Barcelona, based on GIS mapping, as well as correlations with a variety of geo-referenced data sources and the application of different socio-economic variables. The relationship between these two dimensions is key for policymakers in influencing regulatory approaches to collaborative platform activity and mitigating socio-spatial inequalities generated by short term rentals and the platforms that mediate them.
There is little research that analyses the contribution of tourism‐related digital platforms, and particularly Airbnb, to the creation and projection of international destinations' images. This study ...seeks to address this gap by developing a content analysis of the Airbnb Guides to more than 500 global urban neighbourhoods (globalhoods). We analysed Airbnb users' descriptions posted in the period following the Great Recession up to the COVID‐19 pandemic. Content analysis shows how Airbnb projects the images of these globalhoods through a narrative based on creating a perception of authenticity but that finally projects a commodified image of destination identities and their communities.
The introduction of 3D bioprinting to fabricate living constructs with tailored architecture has provided a new paradigm for biofabrication, with the potential to overcome several drawbacks of ...conventional scaffold‐based tissue regeneration strategies. Hydrogel‐based materials are suitable candidates regarding cell biocompatibility but often display poor mechanical properties. Self‐assembling peptides are a promising source of biomaterials to be used as 3D scaffolds based on their similarity to extracellular matrices (structurally and mechanically). In this study, an advanced bioink for biofabrication is presented based on the optimization of a RAD16‐I‐based biomaterial. The strategy followed to build 3D predefined structures by 3D printing is based on an enhancement of bioink viscosity by adding methylcellulose (MC) to a RAD16‐I solution. The resultant constructs display high shape fidelity and stability and embedded human mesenchymal stem cells present high viability after 7 days of culture. Moreover, cells are also able to differentiate to the adipogenic lineage, suggesting the suitability of this novel biomaterial for soft tissue engineering applications.
A bioink based on a self‐assembled peptide and methylcellulose is developed. The addition of methylcellulose allows the bioprinting of the self‐assembled peptide. The bioink exhibits good printability, print fidelity, and biocompatibility. The developed bioink maintains the differentiation potential of the encapsulated cells.
Airbnb, as the world's best-known short-stay rentals' platform, is disrupting tourism everywhere, but studies into its growth and impact have been mostly limited to international urban destinations. ...To cover this gap, this study uses a database of 32,421 Airbnb listings obtained from its website to analyse, using a correlational and a linear regressions analysis, their distribution and nature across Catalonia's 946 municipalities (excluding Barcelona city) and measuring their embeddedness in the formal accommodation sector and in the regulated tourist system in the region. Results show that Airbnb has particularly grown in mature traditional destinations, presenting a predominantly commercial model and playing an additive role. This study also reveals Airbnb as a new actor in the regulated tourist system in Catalonia thanks to the use of a legal figure (HUT) to channel its development, which also gives the platform a great potential for expansion. This study can be useful for municipalities and regulatory agencies' decision-making in those destinations where Airbnb's presence could be particularly disruptive.
Regenerative medicine and disease models have evolved in recent years from two to three dimensions, providing in vitro constructs that are more similar to in vivo tissues. By mimicking native ...tissues, cell‐derived matrices (CDMs) have emerged as new modifiable extracellular matrices for a variety of tissues, allowing researchers to study basic cellular processes in tissue‐like structures, test tissue regeneration approaches, and model disease development. In this review, different fabrication techniques and characterization methods of CDMs are presented and examples of their application in cell behavior studies, tissue regeneration, and disease models are provided. In addition, future guidelines and perspectives in the field of CDMs are discussed.
The engineering of cell‐derived matrices has already evidenced to be a feasible and reproducible approach. Beyond 2D to 3D transition, considering in vivo tissue as inspiration, cell‐derived matrices have the potential to become a serious alternative for tissue regeneration and disease modeling. Their feasibility and plasticity toward varied tissue‐platforms supports their use as the next in vitro gold standard.
This study aims to explore the relations between attachment styles and pain intensity and certain emotional variables (anxiety, depression and alexithymia) in a sample of fibromyalgia patients, in ...comparison with healthy women. Data were collected from 146 women with fibromyalgia and 122 healthy women. The variables studied were attachment style, pain intensity, anxiety, depression and alexithymia dimensions. Patients with fibromyalgia showed lower percentages of secure attachment style (69.9% vs. 86%) whilst showing higher avoidant attachment (19.8% vs. 7.4%), as well as increased numbers of anxious–ambivalent attachment (10.3% vs. 6.6%) than healthy women (X2 = 9.915, p = .007). Also, fibromyalgia patients showed significantly higher scores in two of the insecure attachment factors (p < .000; p = .020) and lower scores on the secure attachment factor (p = .008) in comparison with healthy women. Higher scores of alexithymia were found in women showing anxious–ambivalent and avoidant attachment styles in comparison with those showing a secure attachment style, regardless of the group they belonged to. In fibromyalgia patients, higher anxiety (p = .005) was found among the women with anxious–ambivalent attachment styles (Mean = 15.15; SD = 1.15) in comparison with those with secure attachment style (Mean = 11.18; SD = .45). No relation was found between attachment style and pain intensity. Avoidant attachment seems to carry out a contradictory role and warrants further research. The results found seem to highlight the need for the Attachment–Diathesis Model of Chronic Pain to include attachment styles as a predictor of the emotional experience of pain in fibromyalgia patients.
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