This study reports cytomorphological, histomorphological, and immunological characterization of 608 biopsy cases of canine malignant lymphoma, with epidemiological and clinical data, collected from 7 ...French veterinary pathology laboratories. It compares morphological characteristics of malignant lymphoma in canines, per the updated Kiel classification system, with those reported in humans, per the World Health Organization (WHO) classification system. Of tumors described, 24.5% and 75.5% were classified as low- and high-grade malignant lymphomas, respectively. Presenting clinical signs included generalized or localized lymphadenopathy (82.4%) and extranodal diseases (17.6%) involving the skin (12.34%) and other sites (5.26%). Immunohistochemistry confirmed 63.8% B-cell (CD3–, CD79a+), 35.4% T-cell (CD3+, CD79a–), and 0.8% null-cell (CD3–, CD79a–) lymphomas. Most B-cell cases (38.49%) were of high-grade centroblastic polymorphic subtype; most T-cell cases (8.55%), high-grade pleomorphic mixed and large T-cell lymphoma subtypes. Some B-cell tumors showed morphologic characteristics consistent with follicular lymphomas and marginal zone lymphomas per the Revised European American Classification of Lymphoid Neoplasms and WHO canine classification systems and the WHO human classification system. Unusual high-grade B-cell subtypes included an atypical high-grade small B-cell lymphoma (0.66%), Burkitt-type B-cell lymphoma (1.64%), plasmacytoid lymphoma (0.99%), and mediastinal anaplastic large B-cell lymphoma (0.16%). Unusual T-cell subtypes included a previously undescribed high-grade canine immunoblastic T-cell type (1.15%), a rare low-grade prolymphocytic T-cell lymphoma (0.16%), and a recently described high-grade canine T-cell entity—aggressive granulocytic large-cell lymphoma (0.16%). Marginal zone lymphomas were common (10.86%); follicular lymphomas were rare (0.49%). Canine primary cutaneous malignant lymphoma subtypes were present (11.84%). There was no significant difference between B- and T-cell malignant lymphoma in regard to canine age and sex. A significant overrepresentation of Boxers (24.19%) was found for T-cell lymphomas.
Purpose
To compare adhesion scores, repair strength and histological findings among sublay, onlay and primary repair incisional hernioplasty techniques. Surgical repairs were employed directly on ...healthy animals, without previous hernia induction, to avoid confounding factors related to hernia development.
Methods
Forty Wistar rats were divided into four groups, control, simulation, onlay and sublay. After 42 days, adhesion intensity, tensile strength of the abdominal wall and anatomopathological histological substrate were compared.
Results
SL group presented greater adhesion scores (
p
< 0.0001), higher tensiometric (
p
< 0.0001), and was characterized by more histiocytes, mononuclear cells, macrovacuolar granulomas and type I collagen on histological analysis. Pearson correlation between adhesions and tensiometry, and between tensiometry and neocollagenization showed a strong positive association (
r
= 0.8905 and 0.6757, respectively in SL group,
p
< 0.05).
Conclusion
Mesh positioning in sublay compartment was followed by increased adhesion development and provides a stronger mesh–tissue attachment, in addition, resulted in a different histological profile of the inflammation/repair substrate. The intensity of these findings was directly correlated, suggesting they could be the result of a common biological phenomenon. Our findings indicate that mesh placement following the retromuscular technique generates a superior repair response, and give clues to a better understanding of the superiority of sublay repair in achieving lower recurrence rates. Characterization of the cellular and molecular elements responsible for the superiority of this technique is in our view an essential prerequisite aiming for improvements in the therapeutic options for the treatment of this disease.
The small dimensions of superconducting tunnel junction (STJ) radiation detectors have motivated the development of STJ arrays to increase detection efficiency and statistical accuracy. We have used ...a pulsed UV laser and X-rays to characterize and understand non-uniformities in the response of different pixels in Ta-based STJ arrays. To separate the contributions of the detector and the readout electronics, we have also injected the pulses from an arbitrary waveform generator into the array’s amplifier chain. We discuss the magnitude and the sources of the non-uniformity and approaches to reduce it.
We present direct imaging measurements of charge transport across a 1 cm × 1 cm × 4 mm-thick crystal of high purity silicon (∼15 kΩ-cm) at temperatures of 5 K and 500 mK. We use these data to measure ...lateral diffusion of electrons and holes as a function of the electric field applied along the 111 crystal axis and to verify our low-temperature Monte Carlo software. The range of field strengths in this paper exceed those used in our previous study R. A. Moffatt et al., Appl. Phys. Lett. 114, 032104 (2019) by a factor of 10 and now encompass the region in which some recent silicon dark matter detectors operate R. Agnese et al., Phys. Rev. Lett. 121, 051301 (2018). We also report on a phenomenon of surface charge trapping, which can reduce expected charge collection.
Background. Fracture risk assessment tool (FRAX) index was developed for estimating of the 10-year risk of major or hip osteoporotic fracture. To date, there is insufficient information regarding the ...correlation between FRAX and serum bone turnover markers (BTMs), such as soluble ligand of receptor activator of nuclear factor-κB (sRANKL), osteoprotegerin (OPG), and other molecules related with secondary osteoporosis in rheumatoid arthritis (RA). Therefore, this study is aimed at assessing the correlation between the FRAX and serum levels of sRANKL, OPG, sRANKL/OPG ratio, Dickkopf-1 (DKK-1), and sclerostin (SOST) in RA. Methods. Cross-sectional study included 156 postmenopausal women with RA. Bone mineral density (BMD) was measured at lumbar spine (L1-L4) and total hip using dual-energy X-ray absorptiometry (DXA). RA patients were divided into (A) RA + osteoporosis and (B) RA without osteoporosis. FRAX scores were calculated including the total hip BMD. Serum sRANKL, OPG, DKK-1, and SOST levels were measured by ELISA. Pearson tests were used for assessing the correlation between serum levels of these molecules and FRAX scores in RA. Results. The RA + osteoporosis group had elevated sRANKL levels (p=0.005), higher sRANKL/OPG ratio (p=0.017), decreased DKK-1 (p=0.028), and lower SOST levels (p<0.001). Low total hip BMD correlated with high sRANKL (p=0.001) and sRANKL/OPG ratio (p=0.005). Total hip and lumbar spine BMD correlated with DKK-1 (p=0.009 and p=0.05, respectively) and SOST levels (p<0.001 and p<0.001, respectively). Higher sRANKL levels and sRANKL/OPG ratio correlated with estimated 10-year risk of a major osteoporotic fractures (p=0.003 and p=0.003, respectively) and hip fracture (p=0.002 and p=0.006, respectively). High serum SOST levels were associated with a low estimated 10-year risk of a major osteoporotic fracture (p=0.003) and hip fracture (p=0.009). Conclusion. High sRANKL levels and sRANKL/OPG ratio can be useful to detect a subgroup of RA patients who has an increased 10-year risk of major and hip osteoporotic fractures.
Correlation between morphotypes and prognosis of canine lymphomas presented discordant results in literature, leading to some dilemma for application in clinical practice. The aim of this study was ...to present a systematic review of literature on the prognostic significance of morphotypes in canine lymphomas. Standardized Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) criteria were applied. Retrospective and prospective studies were included. Level of evidence was determined for each study. Some acceptable evidence suggested a significant prognostic impact of morphotypes in canine lymphomas. However, the evidence is not sufficiently robust to determine with precision the most appropriate classification scheme. Updated Kiel and World Health Organization (WHO) classifications seem to remain the most appropriate classification systems with regards to the number of available studies and their levels of evidence. Limitations included lack of randomized control trial, and relative lack of prospective studies available. Current recommended classification of canine lymphoma is the systematic determination of morphotype in each new case. The Updated Kiel and the WHO classifications adapted to dog both remain the schemes with the most valuable interest. Prospective studies in larger population, and international consensus to define precisely each morphotype, are warranted, with application of standardized staging method and treatment.
El objetivo del presente trabajo fue identificar polimorfismos de nucleótido simple (PNSs) en genes de proteínas asociadas a las queratinas (KRTAPs) en alpacas (Vicugna pacos). Se realizó el estudio ...en un total de 34 KRTAPs que se encuentran anotados en el genoma de referencia VicPac3.1 en la base de datos del National Center for Biotechnology Information (NCBI). El genoma de referencia VicPac3.1 de alpaca, nueve genomas (NCBI) y reads de 300 bibliotecas de representación reducida de ADN de alpacas se usaron para comparar secuencias de KRTAPs e identificar PNSs mediante el uso del BLASTN. Se halló la frecuencia del alelo menor (MAF) y la tasa de genotipificacion (TG) de PNSs, mediante el uso de los programas KGD y PLINK. Así como, el Illumina Score para cada PNS, mediante el uso del programa Illumina Design Studio. Se seleccionaron marcadores con MAF ≥ 0.05, TG ≥ 45% por PNS basado en secuencias reads e Illumina Score ≥ 0.6. Se identificaron 67 PNSs ubicados en las regiones intrónicas, exónicas y/o no traducidas de los genes de proteínas asociadas a las queratinas, que cumplen con dichos parámetros. De estos, 35 PNSs fueron incluidos en una micromatriz de 76 mil PNSs de alpaca y 32 PNSs fueron validados en una población de 936 alpacas.
This study aimed to identify single nucleotide polymorphisms (SNPs) in keratin-associated protein genes (KRTAPs), in alpacas (Vicugna pacos). The study was conducted on 34 KRTAPs genes that are annotated in the National Center for Biotechnology Information (NCBI) database. The reference genome VicPac3.1, nine genomes (NCBI) and reads from 300 alpaca reduce representation DNA libraries were used to compare KRTAPs sequences to identify SNPs, using BLASTN. The minor allele frequency (MAF) and the genotyping rate were calculated using KGD and PLINK software. The Illumina Score was calculated with the Illumina Design Studio software for each SNP. Markers with minor allele frequency ≥0.05, a genotyping rate > 45% per SNP based on read sequences and an Illumina Score ≥ 0.6 were selected. Sixty-seven SNPs identified in intron, exon and/or untranslated regions of the keratin-associated protein genes met these parameters. Of these, 35 SNPs were included in the 76K alpaca SNP microarray and 32 SNPs were confirmed in a population of 936 alpacas.
Abstract
Parkinson’s disease (PD) is the second most common chronic degenerative brain disorder in the world preceded by Alzheimer’s disease, affecting one in every hundred people over sixty ...worldwide. This research shows the evaluation of hydrolyzed collagen specimens that emulate the viscoelastic properties of brain tissue. Also, an electromechanical clamp-type device is proposed for data acquisition as well as a graphic interface that displays samples values.
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