In the last few decades, attention on new natural antimicrobial compounds has arisen due to a change in consumer preferences and the increase in the number of resistant microorganisms. Macroalgae ...play a special role in the pursuit of new active molecules as they have been traditionally consumed and are known for their chemical and nutritional composition and their biological properties, including antimicrobial activity. Among the bioactive molecules of algae, proteins and peptides, polysaccharides, polyphenols, polyunsaturated fatty acids and pigments can be highlighted. However, for the complete obtaining and incorporation of these molecules, it is essential to achieve easy, profitable and sustainable recovery of these compounds. For this purpose, novel liquid-liquid and solid-liquid extraction techniques have been studied, such as supercritical, ultrasound, microwave, enzymatic, high pressure, accelerated solvent and intensity pulsed electric fields extraction techniques. Moreover, different applications have been proposed for these compounds, such as preservatives in the food or cosmetic industries, as antibiotics in the pharmaceutical industry, as antibiofilm, antifouling, coating in active packaging, prebiotics or in nanoparticles. This review presents the main antimicrobial potential of macroalgae, their specific bioactive compounds and novel green extraction technologies to efficiently extract them, with emphasis on the antibacterial and antifungal data and their applications.
Fucoxanthin is a pigment present in diverse marine organisms such as micro and macro-algae. The most relevant source of fucoxanthin has been described to be the group of the brown macroalgae, also ...known as Phaeophyceae. The presence of the fucoxanthin, a xanthophyll, found as an accessory pigment in the chloroplasts of the brown algae is responsible for providing them their characteristic color. The market size of this carotenoid, expected to reach US$ 120 million by 2022, reflects its industrial importance, especially remarkable as a food or cosmetic ingredient.
Therefore, it is critical to recognize the main sources of fucoxanthin as well as the most efficient extraction and purification methods that allow obtaining the best production ratio for such a valuable molecule. Throughout this review very different preventive properties of the fucoxanthin have been included, such as antioxidant, anticancer, antiangiogenic, anti-inflammatory, cytoprotective, antiobesity, neuroprotective and its skin protective effects. The stability, bioavailability and toxicity of the fucoxanthin have also been reviewed through diverse biological, in vitro and in vivo assays.
Thus, the main aim of this work is to provide a wide and global vision of the fucoxanthin in terms of productive species, efficient recovery techniques and multiple industrial applications.
The main sources of fucoxanthin are brown algae (Phaeophytes) and some microalgae. Several techniques have been employed to extract this compound, such as maceration extraction (ME), enzyme assisted extraction (EAE), microwave assisted extraction (MAE), pressurized liquid extraction (PLE) or supercritical fluid extraction (SFE). Following the extraction, the identification step has been carried out using different chromatographic and spectroscopic techniques. Fucoxanthin has gained attention in the last decades, due to the wide variety of attributed beneficial activities, such as antioxidant, anti-inflammatory, anticancer or neuroprotective effects. These bioactivities are interesting for its diverse applications in the industry, including the development of innovative pharmaceutical, cosmetic and food products. Display omitted
•Review of fucoxanthin main matrixes and bioactive properties.•Approximation to fucoxanthin extraction and quantification methods.•Review of fucoxanthin stability, bioavailability and toxicity in diverse biological assays.•Content of fucoxanthin in micro and macroalgae (notably in chloroplasts of brown algae).•Fucoxanthin industrial importance (US$ 120 million by 2022) as ingredient (food or cosmetic).
The nuclear radio emission of low-luminosity active galactic nuclei (LLAGNs) is often associated with unresolved cores. In this paper we show that most LLAGNs present extended jet radio emission when ...observed with sufficient angular resolution and sensitivity. They are thus able to power, at least, parsec-scale radio jets. To increase the detection rate of jets in LLAGNs, we analyze subarcsecond resolution data of three low-ionization nuclear emission regions. This yields the detection of extended jet-like radio structures in NGC 1097 and NGC 2911 and the first resolved parsec-scale jet of NGC 4594 (Sombrero). The three sources belong to a sample of nearby LLAGNs for which high-spatial-resolution spectral energy distribution of their core emission is available. This allows us to study their accretion rate and jet power (Q sub(jet)) without drawing on (most) of the ad hoc assumptions usually considered in large statistical surveys. We find that those LLAGNs with large-scale radio jets (> 100 pc) have Q sub(jet) > 10 super(42) erg s super(-1), while the lowest Q sub(jet) correspond to those LLAGNs with parsec-scale (< or =, slant100 pc) jets. The Q sub(jet) is at least as large as the radiated bolometric luminosity for all LLAGN, in agreement with previous statistical studies. Our detection of parsec-scale jets in individual objects further shows that the kinematic jet contribution is equally important in large- or parsec-scale objects. We also find that the Eddington-scaled accretion rate is still highly sub-Eddingtonian (<10 super(-4)) when adding the Q sub(jet) to the total emitted luminosity (radiated plus kinetic). This indicates that LLAGNs are not only inefficient radiators but that they also accrete inefficiently or are very efficient advectors.
•The extracts of Arbutus unedo L. fruits showed high concentration of anthocyanins.•Two different techniques were compared to maximize the extraction.•Heat extraction proved to be the most effective ...method.•Optimal conditions of variables to maximize anthocyanins extraction were found.
The goal of this study was to compare anthocyanin extraction from Arbutus unedo L. fruits, by using two techniques assisted by different energy sources, heat and ultrasound. In order to obtain the conditions that maximize anthocyanin extraction, a response surface methodology was applied using the circumscribed central composite design of three variables with five levels. Three anthocyanin compounds were identified by HPLC-DAD-ESI/MS, being cyanidin-3-glucoside the main molecule. Heat assisted extraction proved to be the most effective method at 5 min, 90 °C and 80% of ethanol, yielding 51.2% of extract, with a total anthocyanin content of 382.4 µg/g dried fruit, and 744.6 µg/g extract. These response values were slightly improved by studying the solid/liquid effect at the optimal conditions in dose-response format, showing steady extraction values from 5 to 40 g/L. Results show the possibility of using A. unedo fruits as a source of anthocyanin compounds for industrial applications.
With more than 240 million people infected, hepatitis B virus (HBV) is a major health concern. The inability to mimic the complexity of the liver using cell lines and regular primary human hepatocyte ...(PHH) cultures pose significant limitations for studying host/pathogen interactions. Here, we describe a 3D microfluidic PHH system permissive to HBV infection, which can be maintained for at least 40 days. This system enables the recapitulation of all steps of the HBV life cycle, including the replication of patient-derived HBV and the maintenance of HBV cccDNA. We show that innate immune and cytokine responses following infection with HBV mimic those observed in HBV-infected patients, thus allowing the dissection of pathways important for immune evasion and validation of biomarkers. Additionally, we demonstrate that the co-culture of PHH with other non-parenchymal cells enables the identification of the cellular origin of immune effectors, thus providing a valuable preclinical platform for HBV research.
We present archival high-spatial-resolution VLA and VLBA data of the nuclei of seven of the nearest and brightest Seyfert galaxies in the Southern Hemisphere. At VLA resolution (∼0.1 arcsec), the ...nuclei of the Seyfert galaxies are unresolved, with the exception of MCG-5-23-16 and NGC 7469, which show a core–jet structure. Three Seyfert nuclei are surrounded by diffuse radio emission related to star-forming regions. VLBA observations with parsec-scale resolution demonstrate that in MRK 1239 the nucleus is clearly resolved into two components separated by ∼30 pc, whereas the nucleus of NGC 3783 is unresolved. Further comparison between VLA and VLBA data of these two sources shows that the flux density at parsec scales is only 20 per cent of that measured by the VLA. This suggests that the radio emission is not concentrated in a single central component, as in elliptical radio galaxies, and an additional low-surface-brightness component must be present. A comparison of Seyfert nuclei with different radio spectra shows that the ‘presence’ of undetected flux on a milliarcsecond scale is common in steep-spectrum objects, whereas in flat-spectrum objects essentially all the radio emission is recovered. In the steep-spectrum objects, this ‘missing’ flux probably results from non-thermal radiation related to active galactic nuclei, perhaps from a jet that is disrupted in Seyfert galaxies because of the denser environment of their spiral hosts.
Azo dyes, including Tartrazine, Sunset Yellow, and Carmoisine, are added to foods to provide color, but they have no value with regard to nutrition, food preservation, or health benefits. Because of ...their availability, affordability, stability, and low cost, and because they provide intense coloration to the product without contributing unwanted flavors, the food industry often prefers to use synthetic azo dyes rather than natural colorants. Food dyes have been tested by regulatory agencies responsible for guaranteeing consumer safety. Nevertheless, the safety of these colorants remains controversial; they have been associated with adverse effects, particularly due to the reduction and cleavage of the azo bond. Here, we review the features, classification, regulation, toxicity, and alternatives to the use of azo dyes in food.
•The availability, affordability, stability and safety of azo dyes make them desirable for food use.•Tartrazine, Sunset Yellow or Carmoisine are the most widely used azo dyes due to their coloring capacity.•The safety of these artificial colorants is controversial due to possible adverse and toxic effects.•Further research on these dyes is needed to provide consistent data, alternatives to their use and safety.
Aquaculture industry exposes fish to acute stress events, such as high stocking density, and a link between stress and higher susceptibility to diseases has been concluded. Several studies have ...demonstrated increased stress tolerance of fish treated with probiotics, but the mechanisms involved have not been elucidated. Shewanella putrefaciens Pdp11 is a strain isolated from healthy gilthead seabream (Sparus aurata L.) and it is considered as probiotics. The aim of this study was to evaluate the effect of the dietary administration of this probiotics on the stress tolerance of Solea senegalensis specimens farmed under high stocking density (PHD) compared to a group fed a commercial diet and farmed under the same conditions (CHD). In addition, during the experiment, a natural infectious outbreak due to Vibrio species affected fish farmed under crowding conditions. Changes in the microbiota and histology of intestine and in the transcription of immune response genes were evaluated at 19 and 30 days of the experiment. Mortality was observed after 9 days of the beginning of the experiment in CHD and PHD groups, it being higher in the CHD group. Fish farmed under crowding stress showed reduced expression of genes at 19 day probiotic feeding. On the contrary, a significant increase in immune related gene expression was detected in CHD fish at 30 day, whereas the gene expression in fish from PHD group was very similar to that showed in specimens fed and farmed with the conventional conditions. In addition, the dietary administration of S. putrefaciens Pdp11 produced an important modulation of the intestinal microbiota, which was significantly correlated with the high number of goblet cells detected in fish fed the probiotic diet.
•Shewanella putrefaciens Pdp11 enhances tolerance to crowding stress in Solea senegalensis.•Dietary administration of S. putrefaciens Pdp11 modulates immune gene transcription.•Some intestinal changes are related to microbiota modulation by S. putrefaciens Pdp11.
We present the discovery of ASASSN-18ey (MAXI J1820+070), a new black hole low-mass X-ray binary (LMXB) discovered by the All-Sky Automated Survey for SuperNovae (ASAS-SN). A week after ASAS-SN ...discovered ASASSN-18ey as an optical transient, it was detected as an X-ray transient by MAXI/GCS. Here, we analyze ASAS-SN and Asteroid Terrestrial-impact Last Alert System pre-outburst optical light curves, finding evidence of intrinsic variability for several years prior to the outburst. While there was no long-term rise leading to the outburst, as has been seen in several other systems, the start of the outburst in the optical preceded that in the X-rays by 7.20 0.97 days. We analyze the spectroscopic evolution of ASASSN-18ey from pre-maximum to >100 days post-maximum. The spectra of ASASSN-18ey exhibit broad, asymmetric, double-peaked H emission. The Bowen blend (λ 4650 ) in the post-maximum spectra shows highly variable double-peaked profiles, likely arising from irradiation of the companion by the accretion disk, typical of low-mass X-ray binaries. The optical and X-ray luminosities of ASASSN-18ey are consistent with black hole low-mass X-ray binaries, both in outburst and quiescence.
•The triangulation of analytical methods improves the detection of errors in judo.•The results contribute to optimizing the knowledge of performance.•Knowing the technical errors perfects feedback ...mechanisms.•Different correctional methods favor the improvement of confidence.
The aim of this article is to identify the factors involved in learning judo based on an observational study of technical errors and their relationships. The analysis was carried out using a combination of a self-generated observational instrument (OI-JUDO-TG) and a recording instrument (developed with the Lince software) using a sample of 78 novice students. In addition, descriptive statistics and sequential analysis with polar coordinates and T-Patterns were obtained using the Hoisan and Theme software program. The implementation of the various analytical methods optimizes the knowledge of performance and the concretion of feedback, like the process of communication between the teacher and the student, that which will activate new neuronal connections in learning. The results confirmed that the most frequent chain of errors during the performance of the technique were as follows: (1) the lack of an initial imbalance, the incorrect placement of the feet, the head and (2) the head after the tsukuri of the technique, as well as the absence of load of the body during this phase and the lack of balance of the performer when finishing the projection. At the initiation of judo, analytical methods are not applied, but the personal experience or the immediate feedback from the teacher is applied. It proposes a new analytical method to learn the technical errors and the different ways to implement their correction. Through the observational analysis generated, intense relationships between the detected errors have been demonstrated, determining the presence of the ones that causes the occurrence of the others.
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