1.
Connexin 36 is Expressed in Beta and Connexins 26 and 32 in Acinar Cells at the End of the Secondary Transition of Mouse Pancreatic Development and Increase During Fetal and Perinatal Life
Pérez-Armendariz, Elia Martha; Cruz-Miguel, Lourdes; Coronel-Cruz, Cristina ...
Anatomical record (Hoboken, N.J. : 2007),
06/2012, Letnik:
295, Številka:
6
Journal Article
To identify when during fetal development connexins (Cxs) 26 (Cx26) 32 (Cx32), and 36 (Cx36) begin to be expressed, as well as to characterize their spatial distribution, real time polymerase chain ...
reaction and immunolabeling studies were performed. Total RNA from mouse pancreases at 13 and 18 days postcoitum (dpc) and 3 days postpartum (dpp) was analyzed. In addition, pancreatic sections of mouse at 13, 14, 15, 16, 18 dpc and 3 dpp and of rat at term were double labeled with either anti‐insulin or anti‐α‐amylase and anti‐Cx26 or ‐Cx32 or ‐Cx36 antibodies and studied with confocal microscopy. From day 13 dpc, Cxs 26, 32, and 36 transcripts were identified and their levels increased with age. At 13–14 dpc, Cxs 26 and 32 were localized in few acinar cells, whereas Cx36 was distributed in small beta cell clumps. From day 14 dpc onwards, the number of labeled cells and relative immunofluorescent reactivity of all three Cxs at junctional membranes of the respective cell types increased. Cxs 26 and 32 colocalized in fetal acinar cells. In rat pancreas at term, a similar connexin distribution was found. Relative Cxs levels evaluated by immunoblotting also increased (two‐fold) in pancreas homogenates from day 18 dpc to 3 dpp. The early cell specific, wide distribution, and age dependent expression of Cxs 26, 32, and 36 during fetal pancreas ontogeny suggests their possible involvement in pancreas differentiation and prenatal maturation. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc., Inc.
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2.
Use of three depression scales for evaluation of pretreatment severity and of improvement after treatment
Senra Rivera, C; Rancaño Pérez, C; Sánchez Cao, E ...
Psychological reports,
10/2000, Letnik:
87, Številka:
2
Journal Article
We investigated the Castilian Spanish versions of three scales (the Hamilton Rating Scale for Depression, the Carroll Rating Scale for Depression, and the Montgomery-Asberg Depression Rating Scale) ...
as to their suitability for classifying major depression patients in four levels of severity prior to treatment with tricyclic antidepressants, and also as to their suitability for classifying these patients in four improvement levels when administered posttreatment. Although the scales behaved quite similarly, each was most efficient at the task for which it had been designed, i.e., severity rating for the Hamilton and Carroll scales, improvement rating for the Montgomery-Asberg scale.
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3.
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