We present the high-quality genome sequence of a ∼45,000-year-old modern human male from Siberia. This individual derives from a population that lived before-or simultaneously with-the separation of ...the populations in western and eastern Eurasia and carries a similar amount of Neanderthal ancestry as present-day Eurasians. However, the genomic segments of Neanderthal ancestry are substantially longer than those observed in present-day individuals, indicating that Neanderthal gene flow into the ancestors of this individual occurred 7,000-13,000 years before he lived. We estimate an autosomal mutation rate of 0.4 × 10(-9) to 0.6 × 10(-9) per site per year, a Y chromosomal mutation rate of 0.7 × 10(-9) to 0.9 × 10(-9) per site per year based on the additional substitutions that have occurred in present-day non-Africans compared to this genome, and a mitochondrial mutation rate of 1.8 × 10(-8) to 3.2 × 10(-8) per site per year based on the age of the bone.
By sequencing 523 ancient humans, we show that the primary source of ancestry in modern South Asians is a prehistoric genetic gradient between people related to early hunter-gatherers of Iran and ...Southeast Asia. After the Indus Valley Civilization's decline, its people mixed with individuals in the southeast to form one of the two main ancestral populations of South Asia, whose direct descendants live in southern India. Simultaneously, they mixed with descendants of Steppe pastoralists who, starting around 4000 years ago, spread via Central Asia to form the other main ancestral population. The Steppe ancestry in South Asia has the same profile as that in Bronze Age Eastern Europe, tracking a movement of people that affected both regions and that likely spread the distinctive features shared between Indo-Iranian and Balto-Slavic languages.
This paper presents new stable carbon (δ13C) and nitrogen (δ15N) isotope data obtained from human and animal remains from the Kamennyi Ambar 5 cemetery (KA-5) (Southeastern Urals, Russian Federation) ...and represents one of the largest stable isotope datasets from a single prehistoric site in the steppes of Central Eurasia. These results are compared with other regional faunal, botanical and archaeological datasets to examine late prehistoric trends in pastoralism and human dietary patterns. The findings of this research emphasize a subsistence regime consisting of broad-spectrum resources that include domestic and wild animal species, wild plants and fish. This study contributes to current knowledge regarding the diversity in isotopic values of human and animal remains and indicates that variation in subsistence was related to distinct local resource biomes and economic strategies. These results suggest a more complex model of late prehistoric subsistence trends in the steppes that emphasizes the need for enhanced micro-regional studies combining environmental, biological, and archaeological datasets. The study presented here also provides information on the most detailed bioarchaeological study of human remains and stable isotopes to date related to the Sintashta archaeological pattern.
•New (δ13C) and (δ15N) stable isotope data from prehistoric human and animal remains are presented.•Findings emphasize a prehistoric subsistence regime consisting of broad-spectrum resources.•Increases awareness to diversity of isotopic values (flora, fauna, fish) in prehistoric Eurasian steppes.•Findings support model of distinct local biomes and variable pastoralist economic strategies.
Objectives
This paper investigates infant feeding practices through stable carbon (δ13C) and nitrogen (δ15N) isotopic analyses of human bone collagen from Kamennyi Ambar 5, a Middle Bronze Age ...cemetery located in central Eurasia. The results presented are unique for the time period and region, as few cemeteries have been excavated to reveal a demographic cross‐section of the population. Studies of weaning among pastoral societies are infrequent and this research adds to our knowledge of the timing, potential supplementary foods, and cessation of breastfeeding practices.
Materials and methods
Samples were collected from 41 subadults (<15 years) and 27 adults (15+ years). Isotopic reference sets from adult humans as well as faunal remains were utilized as these form the primary and complementary foods fed to infants.
Results
Slight shifts in δ13C and δ15N values revealed that weaning was a multi‐stage process (breastfeeding, weaning, and complete cessation of nursing) that began at 6 months of age, occurred over several years of early childhood, and was completed by 4 years of age.
Discussion
Our results indicate that weaning was a multi‐stage process that was unique among late prehistoric pastoralist groups in Eurasia that were dependent on milk products as a supplementary food. Our discussion centers on supporting this hypothesis with modern information on central and east Eurasian herding societies including the age at which complementary foods are introduced, the types of complementary foods, and the timing of the cessation of breastfeeding. Integral to this work is the nature of pastoral economies and their dependence on animal products, the impact of complementary foods on nutrition and health, and how milk processing may have affected nutrition content and digestibility of foods. This research on Eurasian pastoralists provides insights into the complexities of weaning among prehistoric pastoral societies as well as the potential for different complementary foods to be incorporated into infant diets in the past.
In order to investigate the genetic features of ancient West Siberian people of the Middle Ages, we studied ancient DNA from bone remains excavated from two archeological sites in West Siberia: ...Saigatinsky 6 (eighth to eleventh centuries) and Zeleny Yar (thirteenth century). Polymerase chain reaction amplification and nucleotide sequencing of mitochondrial DNA (mtDNA) succeeded for 9 of 67 specimens examined, and the sequences were assigned to mtDNA haplogroups B4, C4, G2, H and U. This distribution pattern of mtDNA haplogroups in medieval West Siberian people was similar to those previously reported in modern populations living in West Siberia, such as the Mansi, Ket and Nganasan. Exact tests of population differentiation showed no significant differences between the medieval people and modern populations in West Siberia. The findings suggest that some medieval West Siberian people analyzed in the present study are included in direct ancestral lineages of modern populations native to West Siberia.
Objectives
We tested the hypothesis that the purported unstable climate in the South Urals region during the Middle Bronze Age (MBA) resulted in health instability and social stress as evidenced by ...skeletal response.
Methods
The skeletal sample (n = 99) derived from Kamennyi Ambar 5 (KA‐5), a MBA kurgan cemetery (2040‐1730 cal. BCE, 2 sigma) associated with the Sintashta culture. Skeletal stress indicators assessed included cribra orbitalia, porotic hyperostosis, dental enamel hypoplasia, and tibia periosteal new bone growth. Dental disease (caries, abscess, calculus, and periodontitis) and trauma were scored. Results were compared to regional data from the nearby Samara Valley, spanning the Early to Late Bronze Age (EBA, LBA).
Results
Lesions were minimal for the KA‐5 and MBA‐LBA groups except for periodontitis and dental calculus. No unambiguous weapon injuries or injuries associated with violence were observed for the KA‐5 group; few injuries occurred at other sites. Subadults (<18 years) formed the majority of each sample. At KA‐5, subadults accounted for 75% of the sample with 10% (n = 10) estimated to be 14‐18 years of age.
Conclusions
Skeletal stress markers and injuries were uncommon among the KA‐5 and regional groups, but a MBA‐LBA high subadult mortality indicates elevated frailty levels and inability to survive acute illnesses. Following an optimal weaning program, subadults were at risk for physiological insult and many succumbed. Only a small number of individuals attained biological maturity during the MBA, suggesting that a fast life history was an adaptive regional response to a less hospitable and perhaps unstable environment.
PDF
