Lactating Holstein cows (n=141) were synchronized to receive their first timed artificial insemination (TAI). Blood and milk samples were collected 25 and 32d after TAI, and pregnancy status was ...determined 32d after TAI using transrectal ultrasonography. Cows diagnosed pregnant with singletons (n=48) continued the experiment in which blood and milk samples were collected and pregnancy status was assessed weekly using transrectal ultrasonography from 39 to 102d after TAI. Plasma and milk samples were assayed for pregnancy-associated glycoprotein (PAG) levels using commercial ELISA kits. Compared to ultrasonography, accuracy was 92% for the plasma PAG ELISA test and 89% for the milk PAG ELISA test 32d after TAI. Plasma and milk PAG levels for pregnant cows increased from 25d to an early peak 32d after TAI. Plasma and milk PAG levels then decreased from 32d after TAI to a nadir from 53 to 60d after TAI for the plasma PAG assay and from 46 to 67d after TAI for the milk PAG assay followed by an increase from 74 to 102d after TAI. Overall, plasma PAG levels were approximately 2-fold greater compared with milk PAG levels, and primiparous cows had greater PAG levels in plasma and milk compared with multiparous cows. The incidence of pregnancy loss from 32 to 102d after TAI based on ultrasonography was 13% for cows diagnosed with singleton pregnancies, and plasma and milk PAG levels decreased to nonpregnant levels within 7 to 14d after pregnancy loss. Both plasma and milk PAG levels were negatively correlated with milk production for both primiparous and multiparous cows. We conclude that stage of gestation, parity, pregnancy loss, and milk production were associated with plasma and milk PAG levels after TAI similarly. Based on plasma and milk PAG profiles, the optimal time to conduct a first pregnancy diagnosis is around 32d after AI, coinciding with an early peak in PAG levels. Because of the occurrence of pregnancy loss, all pregnant cows should be retested 74d after AI or later when plasma and milk PAG levels in pregnant cows have rebounded from their nadir.
Glucose and mannose have a different degree of sweetness, implying different affinity to the sweet taste receptor. While the receptor structure is still undefined, there are several geometrical ...models for their binding mechanism. A detailed study of the hydration structure of sugars with known degree of sweetness is bound to provide information on the accuracy of such models. Our neutron diffraction study on the hydration of glucose and mannose show that both α- and β-glucose form strong hydrogen bonds with water, and that the steric hindrance of their first hydration shell matches the receptor geometrical model. The α-anomer of mannose has a similar, well-defined first hydration shell, but with fewer and weaker hydrogen bonds compared to glucose. Conversely, the hydration shell of β-mannose (reported as bitter) does not match the receptor geometrical model. These findings suggest a link between the hydration shell of sugars and their degree of sweetness.
The replacement of toxic solvents with greener alternatives in Heck–Cassar–Sonogashira (HCS) cross-couplings was investigated. The fine-tuning of the HCS protocol allowed to achieve complete ...conversions and high speed under mild conditions. N-Hydroxyethylpyrrolidone (HEP) gave the best results. Moreover, the methodology was successfully applied to the synthesis of an intermediate of the anticancer drug Erlotinib, demonstrating the versatility of the new green protocol.
Although hormonal synchronization programs can improve reproductive efficiency of dairy herds, some farmers question the economics of these programs based on the upfront cost of hormonal treatments ...as opposed to the economic value of the resulting reproductive performance. Our aim was to compare the economic impact of reproductive management programs that incorporate varying degrees of detection of estrus and timed artificial insemination (AI) in dairy herds with year-round calving in confinement total mixed ration systems. A reproductive economic analysis simulation model was used to compare the economic impact of pairs of reproductive management programs. We simulated sets of scenarios for 2 analyses. In the first analysis, we calculated the economic impact of switching from a Presynch-Ovsynch program to a Double-Ovsynch program that included a second PGF2α treatment during the Breeding-Ovsynch portion of the program (Double-Ovsynch+PGF). In the second analysis, we conducted a break-even analysis in which the cost of hormonal treatments was incrementally increased within various reproductive management programs. Our analyses revealed that a Double-Ovsynch+PGF program, the most intensive program evaluated, was more profitable than other programs compared, including a Presynch-Ovsynch program with 100% timed AI or a Presynch-Ovsynch program that incorporated detection of estrus, despite the higher upfront cost incurred by using more hormonal treatments. This advantage remained until the cost of hormones was increased 5 to 14 times current US market prices and 2 to 6 times current European market prices. The cost of GnRH had a greater impact on net profit gain than the cost of PGF2α. In conclusion, more intensive reproductive programs that use more hormonal treatments but result in substantially increased reproductive performance are more profitable than less intensive programs and remain so even if hormone prices are unusually high.
The solvation of caffeine in water is examined with neutron diffraction experiments at 353 K. The experimental data, obtained by taking advantage of isotopic H/D substitution in water, were analyzed ...by empirical potential structure refinement (EPSR) in order to extract partial structure factors and site–site radial distribution functions. In parallel, molecular dynamics (MD) simulations were carried out to interpret the data and gain insight into the intermolecular interactions in the solutions and the solvation process. The results obtained with the two approaches evidence differences in the individual radial distribution functions, although both confirm the presence of caffeine stacks at this temperature. The two approaches point to different accessibility of water to the caffeine sites due to different stacking configurations.
Dystocic parturitions have an adverse impact on animal productivity and therefore the profitability of the farm. In this regard, accurate prediction of calving is essential since it allows for ...efficient and prompt assistance of the dam and the calf. Numerous approaches to predict parturition have been studied, among these, measurement of intravaginal temperature (IVT) is the most effective method at the field level. Thus, objectives of this experiment were, 1) to find an IVT cut-off to predict calving within 24 h, and 2) to clarify the use of IVT as an automated method of calving detection in housed beef cows. A commercial intravaginal electronic device (Medria Vel’Phone®) with a sensor that measures the IVT every 12 h was used. Piedmontese cows (n = 211; 27 primiparous and 184 multiparous) were included in this study. One-way analysis of variance was used to assess the temperature differences at 0, 12, 24, 36, 48 and 60 h before parturition. Receiving operator characteristic curves were built to determine the temperature cut-off which predicts calving within 24 h with the highest summation of sensitivity (Se) and specificity (Sp). Binomial logistic regression models were computed to identify factors that may affect the IVT before calving. Mean gestation length was 291.5 ± 13.7 d (primiparous, 292 ± 14.1 d; multiparous, 289 ± 9.2 d). A decrease (P < 0.001) in the average IVT was found from 60 h before calving until the expulsion of the IVT device. A significant (P < 0.05) reduction in the IVT was noticeable from 24 h before until parturition. The IVT drop to predict parturition 24 h before calving was 0.21 °C (area under the curve AUC = 0.72; Se = 66%, Sp = 76%). Furthermore, the IVT cut-off value to predict parturition within 24 h was 38.2 °C (AUC = 0.89; Se = 86%, Sp = 91%). None of the evaluated fixed effects (parity, dystocia, season or length of gestation) affected (P ˃ 0.05) the IVT variation from 60 h before and up to calving. To conclude, the IVT average seems to be a better parameter than the drop in temperature to predict parturition within 24 h. In this regard, a cut-off of 38.2 °C showed a high Se and Sp for predicting calving. This study demonstrates the usefulness of a commercially available device to predict calving to improve management in stabled beef farms.
•An intravaginal temperature cut off to predict partum is studied.•IVT cut-off works better than the IVT drop to predict the parturition within 24 h.•Accurate partum prediction can help to assist the cow at the delivery time.•Vel’Phone®, is useful to predict calving to improve management in farms.
Cross-sectional with follow-up case-control component.
To measure the prevalence of back pain (BP) and back pain exacerbations, describe BP features and functional impairment, estimate BP-related ...lost productive time (LPT) and costs, and assess the relation between pain exacerbations and lost productive time.
BP is associated with substantial lost work time. However, little is known about the extent to which BP with or without exacerbation explains lost work time.
A national telephone survey of the U.S. workforce identified 320 workers 40 to 65 years of age with BP defined by NHANES I criteria and 91 matched non-BP controls. Participants self-reported pain characteristics, lost productive time (absenteeism and presenteeism) in the previous 2 weeks, activity limitations, and demographics. A population-weighting adjustment was applied to estimates to account for selection bias and ensure that estimates of certain sample demographic subgroups' totals conformed to the Current Population Survey.
The 2-week period prevalence of BP was 15.1%; 42% of workers with BP experienced pain exacerbations. BP prevalence was associated with demographic factors, but BP exacerbations were not. BP was reported by 42.6% of all workers. Workers with exacerbations reported more days with BP than those without exacerbations. Workers with exacerbations were significantly more likely than those without such exacerbations to report activity limitation (88.4% vs. 60.7%; P < 0.0001) and BP-related LPT (22.1% vs. 13.0%; P = 0.0259). BP in workers 40 to 65 years of age costs employers an estimated $7.4 billion/year. Workers with BP exacerbations account for 71.6% of this cost.
Workers with BP exacerbations account for a disproportionate share of the cost of BP-related lost productive time.
Modern architectural concept of “vertical forest” boosts the tendency of including permeable and green spaces in buildings and its surroundings. However, whilst green spaces generally make buildings ...and cities more ecofriendly, it is insufficiently known to which extent the surrounding environment and the vegetation may positively or negatively influence the pedestrian-level wind comfort quality of buildings amidst historical and well-settled urban layouts. This was the scope of the present study for which wind-tunnel (WT) testing and 3D RANS simulations were performed on two facing towers in Medellìn (Colombia), in order to (i) investigate the wind field and the pedestrian-level wind comfort around the towers; (ii) to compare the performance of WT and 3D RANS for wind comfort analysis; (iii) to evaluate the impact of the environment surrounding the two towers and (iv) the mitigation effects of vegetation on wind comfort. WT tests were performed on a scaled model (1:250) without surrounding, for 12 wind directions and 3 sets of measurement positions. The same case study without (i.e. CFDs) and with (i.e. CFDd) surrounding environment was simulated by 3D RANS. Next, an implemented CFDd case including vegetation (i.e., CFDd-V) was simulated for three LAD values. Overall, the surrounding had a significant impact on wind comfort assessment. The CFDd case showed a better-quality class than WT and CFDs. The CFDs and WT case showed a satisfactory agreement and the similar comfort quality class. The vegetation provided a remarkable improvement of comfort quality class mainly for set 1 and 2.
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•A newly designed tower with vertical green park and permeable spaces is presented.•Pedestrian-level wind comfort with/without environment and vegetation is analyzed.•Wind-tunnel tests and RANS simulations are used for predicting the wind flow field.•The surrounding environment plays a key role in pedestrian-level wind comfort study.•Vegetation can improve the comfort in permeable spaces highly exposed to winds.
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