The state of Puebla has the social marginalization and ecological conditions for the transmission of infectious agents to be effective. Until a few years ago, the state of Puebla was considered ...non-endemic to the presence of Trypanosoma cruzi as there are no official reports of chronic cases. The objective of this work was to carry out a preliminary study on the prevalence of anti-T. cruzi antibodies in rural areas of the Huatlatlauca municipality in the Mixtec sierra of the state of Puebla.
A total of 196 serum samples from 12 rural localities were tested by using four tests: two enzyme-linked immunosorbent assays, an xenodiagnoses and PCR assay.
Overall, 28 (14.2%) of 196 samples were positive for T. cruzi by ≥ 2 tests (95% CI 6.6-20.8%). Our results suggested that the municipality of Huatlatlauca in the Mixteca Sierra of the state of Puebla is an area with endemic potential for the disease with a high prevalence rate in the adult population and with cases in newborns, these high transmission rates are probably associated with problems of congenital and vector transmission.
Additional studies should be conducted to generate adequate campaigns for the control of Chagas disease in this area.
5-Azido-3-oxa-pentyl beta-D-galactopyranoside was prepared from diethylene glycol monochlorohydrin and used as a model of oligosaccharide hapten. After deprotection, a series of amides bearing ...thiophilic groups had been obtained through the terminal amino function and essayed in coupling reactions with thiolated BSA. Also several Lewis human blood group oligosaccharides had been conjugated with thiolated BSA demonstrating the usefulness of the methodology.
Trastornos temporomandibulares en adictos al qat Guerra Cobián, Orlando; Pupo Triguero, Raúl J; Sarracent Pérez, Humberto ...
Revista Cubana de estomatologia,
12/2012, Letnik:
49, Številka:
4
Journal Article
Recenzirano
Odprti dostop
Introducción: los trastornos temporomandibulares se asocian frecuentemente al hábito de masticar qat, pero se carece de estudios que detallan sus particularidades, por lo que se realiza este trabajo, ...con los objetivos de determinar la prevalencia de trastornos temporomandibulares en pacientes adictos al qat, identificar los hallazgos clínicos- radiográficos asociados y clasificar los trastornos temporomandibulares presentes en la población estudiada. Métodos: se realizó un estudio prospectivo, descriptivo, de corte transversal, en una muestra de 352 pacientes, del total de pacientes adictos, atendidos en el Departamento de Cirugía, Colegio Dental. Ibb,Yemen en el periodo octubre 2010-abril 2011. Se determinaron los hallazgos clínicos y radiográficos presentes, y se diagnosticó el tipo de trastorno temporomandibular. Resultados: se evidenció que estaban afectados por trastornos temporomandibulares el 55,7 % de los pacientes. Se encontró dolor articular agudo en 14,1 % y aplanamiento condilar anterior en 35,6 %. Conclusiones: los trastornos temporomandibulares afectaron más de la mitad de la población estudiada, adicta al qat. Los desórdenes en la relación cóndilo-disco fueron los más encontrados (41 %). El aplanamiento condilar anterior dominó en el análisis radiográfico.
Se demostró mediante un ensayo de incorporación de bromodeoxiuridina (BrdU), que un anticuerpo monoclonal murino, de la subclase IgG1 contra la proteína de superficie SAG2 de Toxoplasma gondii, es ...capaz de reducir la invasión y multiplicación del parásito en células de la línea altamente diferenciada secretora de mucina HT29-18N2, proveniente de un adenocarcinoma de colon humano. Este resultado demuestra la importancia de la proteína de superficie SAG2 de T. gondii en la invasión y posterior multiplicación del parásito en la célula hospedera.
Con el objetivo de determinar los niveles de IgA en las secreciones, los autores se propusieron obtener anticuerpos monoclonales (AcMs) contra esta inmunoglobulina y demostrar su posible utilización ...en el desarrollo de métodos de cuantificación. Se obtuvieron 6 AcM, 1 contra el componente secretor (CS) y 5 contra cadena pesada (CP) de la inmunoglobulina A (IgA) humana. Se utilizó como inmunógeno IgA purificada a partir de calostro humano. A 2 AcMs, uno contra CS y otro contra CP se les determinó el isotipo de inmunoglobulina (IgG2a e IgG1, respectivamente), se les realizó electroforesis en gel de poliacrilamida en presencia de duodecilsulfato de sodio y b- mercaptoetanol, e inmunotransferencia para la determinación de su especificidad. Se detectó IgA secretora en muestras de saliva comparando el AcM anti-CS obtenido en el laboratorio del Instituto de Medicina Tropical "Pedro Kourí" (IPK) con uno comercial (Sigma Chemical Co.) y se obtuvo similitud de comportamiento. El AcM contra CS (IPK) se utilizó para detectar IgA específica a LPS ogawa en personas vacunadas con una cepa atenuada de Vibrio cholerae y se encontraron diferencias evidentes entre los niveles antes y después de la inmunización. El AcM anti-CP fue utilizado para la confección de un sistema simulado, tipo ELISA. El límite de detectabilidad fue de 9,89 ng/mL.
Introducción: los trastornos temporomandibulares se asocian frecuentemente al hábito de masticar qat, pero se carece de estudios que detallan sus particularidades, por lo que se realiza este trabajo, ...con los objetivos de determinar la prevalencia de trastornos temporomandibulares en pacientes adictos al qat, identificar los hallazgos clínicos- radiográficos asociados y clasificar los trastornos temporomandibulares presentes en la población estudiada. Métodos: se realizó un estudio prospectivo, descriptivo, de corte transversal, en una muestra de 352 pacientes, del total de pacientes adictos, atendidos en el Departamento de Cirugía, Colegio Dental. Ibb,Yemen en el periodo octubre 2010-abril 2011. Se determinaron los hallazgos clínicos y radiográficos presentes, y se diagnosticó el tipo de trastorno temporomandibular. Resultados: se evidenció que estaban afectados por trastornos temporomandibulares el 55,7 % de los pacientes. Se encontró dolor articular agudo en 14,1 % y aplanamiento condilar anterior en 35,6 %. Conclusiones: los trastornos temporomandibulares afectaron más de la mitad de la población estudiada, adicta al qat. Los desórdenes en la relación cóndilo-disco fueron los más encontrados (41 %). El aplanamiento condilar anterior dominó en el análisis radiográfico.Introduction: the temporomandibular disorders has been associated frequently to the habit of chewing qat, but in previous studies, there are lack of specific details of this problem, for this reason this study is carried out ,to determine it prevalence, to identify clinical and radiografical findings associated , and to classify the temporomandibular disorders observed in the sample. Methods: a cross-sectional, descriptive and prospective study was done, in a sample of 352 patients of the total qat addicted patients which came to the Department of surgery, Dentistry College .Ibb Yemen October2010-April 2011. The clinical and radiographical findings were recorded and the temporomandibular disorders were classified. Results: 55.7% of patients were affected of temporomandibular disorders. Acute Joint pain (14.1) and anterior condylar flattening (35.6%) were the most frequents. Conclusions: the Temporomandibular disorders affected more than half of the population of studied qat addicted population, with prevalence of disorders in the disc-condyle relation (41%).The anterior condylar flattening was very evident.
Black Sigatoka caused by Mycosphaerella fijiensis Morelet is the most dangerous and devastating disease of banana around the world. Disease control is carried out by integrating cultural, genetic and ...chemical measures. Mycosphaerella musicola has been replaced by M. fijiensis wherever Black Sigatoka has been introduced in America and elsewhere, and remains as a significant problem at sites located at relatively high altitudes. To optimize fungicide applications for disease management a presymptomatic quantification of fungal proteins in leaves is desirable. In this study, we describe the generation of a mouse monoclonal antibody (Mab) reactive to a high-molecular weight antigens from M. fijiensis mycelial single ascospore in vitro culture, but not reactive to mycelial antigens from M. musicola, M. musae and M. minima single ascospore cultures. A rabbit-specific polyclonal antibody against the same M. fijiensis antigen, reactive also to fungal secreted proteins, was able to discriminate naturally M. fijiensis infected from healthy leaves as well as other concomitant fungi. The Mab was used as a capturing reagent and the polyclonal preparation as a second antibody for a triple antibody enzyme-linked immunosorbent assay able to quantify mycelial protein antigens in a range of 10-40 μg / ml and differentiate it from healthy banana leaf extracts. The aim of this study was the analytical setting up of a Mab-based immunoassay for the quantification of mycelial and secreted proteins of M. fijiensis. It is intended for the further establishment and optimization of an asymptomatic leaf assay for an improved forecast and warning system applied to Black Sigatoka disease management.
In order to determine the IgA levels in secretions, the authors engaged themselves to obtain monoclonal antibodies against this immunoglobulin and to show their possible utilization in the ...development of quantitation methods. 6 monoclonal antibodies were obtained: one against the secretory component (SC) and five against the heavy-chain (HC) of IgA. IgA purified from human colostrum was used as immunogen (HC). The immunoglobulin isotypes (IgG2a and IgG1) were determined in 2 monoclonal antibodies, one against SC and the other against HC. Polyacrylamide gel electrophoresis was performed in the presence of sodium dodecyl sulphate and b-mercapto-ethanol. Immunotransference was also carried out to determine their specificity. Secretory IgA was detected in saliva specimens. The anti-SC monoclonal antibody obtained in-the laboratory of the "Pedro Kourí" Institute of Tropical Medicine (IPK) was compared with a commercial one (Sigma Chemical Co.), and a similar behaviour was observed. The monoclonal antibody against SC (IPK) was used to detect IgA specific to LPS ogawa in persons vaccinated with an attenuated strain of Vibrio cholerae. Marked differences were found between the levels before and after immunization. The anti-HC monoclonal antibody was utilized to make an ELISA-like simulated system. The detection limit was 9.89 ng/mL.
The mechanism of inhibitory action of disodium cromoglycate (DSCG) and Ketotifen (Ke) on smooth muscle against various spasmogens remains uncertain. In an attempt to elucidate it, we have studied the ...effects of both drugs on cumulative dose response curves (CDRC) to CaCl2 in guinea pig ileum. We found that they induce a shift to the right and a non surmountable depression of the maximal response of these CDRC which suggests non competitive antagonism. Furthermore, DSCG inhibits 45Ca++ uptake by guinea pig ileum after acetylcholine stimulation which constitutes strong evidence that inhibition of calcium ion utilization by smooth muscle cells plays a major role in the mode of action of DSCG.
