Laparoscopic sleeve gastrectomy(LSG) is the most popular bariatric surgery worldwide. Postoperative de-novo acid reflux is one of the major common complications of the procedure. Different additive ...anti-reflux surgical techniques have been tried to decrease the complication although no favorable outcome is obtained. This study was conducted to evaluate effects of concurrent cruroplasty during LSG on postoperative de-novo acid reflux incidence rate.
In current participant-blinded randomised controlled trial total of 80 subjects who were candidate for LSG were enrolled from the September 2018 to the December 2019. Following matching patients by gender and age, simple randomization method was held to allocate participants to LSG alone and LSG + cruroplasty groups with equal 40 members in each. Demographic data, length of hospital stay, and operation time was registered. Presence of acid reflux was looked by using gastroesophageal reflux disease-health related quality of life(GERD-HRQL) questionnaire prior and 6 months after surgery in follow-up visit.
Finally 12/28 and 14/26 male/females with 38.5 ± 10.7 and 39.7 ± 8.2 years of age were recruited in LSG alone and LSG + cruroplasty, respectively.(p > 0.05) The length of operative time was significantly shorter in LSG alone(p < 0.01) although no obvious difference was existed in length of hospital stay between groups.(p = 0.7) Postoperative de-novo acid reflux also was not considerably lesser after cruroplasty compared with controls.(p = 0.1) The GERD-HRQL scores were not remarkable between subjects of study groups.(p > 0.05).
Equipping LSG with concurrent cruroplasty to diminish postoperative de-novo gastroesophageal acid reflux is not effective and not recommended in absence of other indications.
•Data of incidence and reinforcement of diaphragmatic crura to inhibit de-novo GERD after LSG is varied and insufficient.•Adding cruroplasty to LSG for preventing de-novo gastroesophageal reflux is not advised.•It is recommended to focus researches on details of surgical technique for LSG to prevent postoperative reflux.
Biliary atresia (BA) is the primary cause of neonatal jaundice with various pathological mechanisms. Many BA patients may experience progressive liver dysfunction and eventually need a liver ...transplant. Therefore, identifying potential non-invasive biomarkers for BA is crucial. miR-122, the most abundant microRNA in the liver, plays significant roles in different liver diseases. This study aimed to assess miR-122 levels in BA patients. Eighteen patients with biliary atresia were selected at random from the Shiraz Pediatric Liver Cirrhosis Cohort Study (SPLCCS), along with 18 healthy controls. Blood samples were collected, and biochemical parameters (such as liver function tests) were measured. Quantitative reverse-transcription PCR (RT-PCR) was conducted on serum samples from both the case and control groups to analyze miR-122 levels. The study results indicated that serum miR-122 expression in BA patients was elevated compared to the control group, although it did not reach statistical significance. Additionally, no correlation was found between miR-122 expression and serum levels of liver enzymes or other laboratory findings in BA cases. miR-122 could be a potential target for diagnosing BA; however, further research with a larger population is necessary to determine if miR-122 could serve as a useful biomarker for diagnosing BA.Biliary atresia (BA) is the primary cause of neonatal jaundice with various pathological mechanisms. Many BA patients may experience progressive liver dysfunction and eventually need a liver transplant. Therefore, identifying potential non-invasive biomarkers for BA is crucial. miR-122, the most abundant microRNA in the liver, plays significant roles in different liver diseases. This study aimed to assess miR-122 levels in BA patients. Eighteen patients with biliary atresia were selected at random from the Shiraz Pediatric Liver Cirrhosis Cohort Study (SPLCCS), along with 18 healthy controls. Blood samples were collected, and biochemical parameters (such as liver function tests) were measured. Quantitative reverse-transcription PCR (RT-PCR) was conducted on serum samples from both the case and control groups to analyze miR-122 levels. The study results indicated that serum miR-122 expression in BA patients was elevated compared to the control group, although it did not reach statistical significance. Additionally, no correlation was found between miR-122 expression and serum levels of liver enzymes or other laboratory findings in BA cases. miR-122 could be a potential target for diagnosing BA; however, further research with a larger population is necessary to determine if miR-122 could serve as a useful biomarker for diagnosing BA.
Various traditional dairy products could be considered as an abundant source for Isolation/collection of new lactic acid bacteria (LAB) with unique characteristics. The present researchaimed to ...investigate the morphological, biochemical and genotypic characterization of dominant lactic acid bacteria that were isolated from traditional dairy products in Iranian tribes . A total of 75 samples of traditional yogurt, doogh from dairy units in Fars province were randomly collected. Isolation of lactic acid bacteria, biochemical and genotypic identification were conducted. Totally, 157 LAB isolates were selected. Cocci and rod shape LAB were 53.50% and 46.49%, respectively. Biochemical tests showed the occurrence of 40.6% of the strains were Streptococcus, 2.25% of the strains were enterococci and 20.3% of the strains were lactococci. Most isolated lactobacilli were related to Lactobacillus plantarum and Lactobacillus bulgaricus. Genetically the presence of the following species was verified: Lactococcus lactis subsp. cremoris, Lactobacillus plantarum, Streptococcus thermophilus, Lactobacillus fermentum, Lactococcus lactis subsp. lactis, Lactobacillus bulgaricus, Lactobacillus helveticus, and Lactobacillus casei. The current study showed that thestrains which were isolated from traditional dairy products were not only appropriatefor use as starter adjuncts or cultures, but also they may provide a valuable gene pool for research and production of commercial starters with specific traits.
Matrix metalloproteinase 7 (MMP7) has been suggested as a promising biomarker in diagnosing biliary atresia (BA). This study aimed to assess the diagnostic accuracy of serum MMP7 in BA in the Middle ...Eastern population.
In this cross-sectional study, neonates and infants with direct hyperbilirubinemia admitted to Namazi referral hospital, Shiraz, Iran, were studied. Baseline demographic and clinical characteristics and blood samples were obtained on admission. MMP7 serum concentration was measured using an enzyme-linked immunosorbent assay (ZellBio GmbH, Ulm, Germany).
44 infants with a mean age of 65.59 days were studied. Of these patients, 13 cases were diagnosed with BA, and 31 cases' cholestasis related to other etiologies. Serum MMP7 concertation was 2.13 ng/mL in the BA group and 1.85 ng/mL in the non-BA group. MMP7 was significantly higher in those presented with either dark urine or acholic stool. The predictive performance capability of the MMP7 was not significant in the discrimination of BA from the non-BA group based on receiver operating characteristic curve analysis (area under curve: 0.6, 95% confidence interval: 0.45–0.75). In the optimal cut of point 1.9, the sensitivity and specificity were 84.6% and 45.1%, respectively. Further combination of MMP7 with Gamma-glutamyl transferase (GGT), alkaline phosphatase, direct and total bilirubin, and dark urine or acholic stool was not remarkably boosted the diagnostic accuracy of the test. Interestingly, GGT at a cut-off point of 230 U/L was 84.6% sensitive and 90.3% specific for BA.
Our results are not consistent with previous studies on this subject. Considering more conventional and available tests like GGT besides conducting future studies with greater samples and different geographical areas is recommended.
Background: Complementary and alternative medicine is defined as “diagnosis, prevention, and treatment, which complements mainstream medicine by contributing to a common whole, by satisfying a demand ...not met by belief or by diversifying the conceptual frameworks of medicine”. Alternative medicine may include acupuncture, homeopathy, phytotherapy, leech therapy, and other methods. According to the public popularity of these methods, we conducted this study to investigate the views of the public about these treatments. Methods: To perform this study, we designed a questionnaire scoring on a 10 five-point Likert scale. The goal of the study was explained to patients before filling out the questionnaire. Results: In this research, 100 cases with an average age of 30±8.47 years were studied. One percent of the studied population was uneducated, 41% had a diploma, and 58% had a university degree. Respectively, 28%, 10%, 40%, 15% of the participants expressed their confidence in Iranian traditional medicine as follows: very high, high, medium, and low, and 7% did not believe in alternative medicine. Also, 24% of respondents had used at least one of the methods of alternative medicine, like leech therapy. In addition, 33% usually and 42% sometimes used herbal tea for relieving their physical and mental illness without consulting the physician, and 25% never used it without consulting. According to the opinion of respondents, 69% believed that both modern and traditional medicine is effective, 10% expressed that modern medicine is more effective than traditional medicine, 9% were not familiar with traditional medicine, and 7% stated that traditional medicine is more effective than modern medicine. Conclusion: According to the results, most of the participants believed in alternative methods, especially along with modern medicine. For this reason, accurate and scientific education about alternative methods seems necessary for the healthcare team and the public.
Nanotechnology could be a very important field of recent research dealing with medical, industrial, environment. Silver nanoparticles are the topics of researchers because of their distinctive ...properties (size, shape depending optical, antimicrobial, and electrical properties). The synthesis of safe nanoparticles by biological organisms is recommended due to the environmental friendliness and low costs compared to physical and chemical methods. Recently, researchers tend to use a biological synthesis of nanoparticles by biological methods such as plants. This research aimed to study on the rainfall level on the biosynthesis of silver nanoparticles by Lamiaceae family plants in different regions of Iran. T-test student was used to examining the relationship between the size of nanoparticles and rainfall, using spss version 22 software. The study shows phenol concentration in low rainfall areas is more than high rainfall area, which is the main factor for the synthesis of nanoparticles. The result of the study demonstrated that the highest size of synthesized nanoparticle is 50 nm and the lowest size 10 nm, which are related to high rainfall and low rainfall regions respectively. The results showed the relationship between synthetic nanoparticles sizes and rainfall are significant p-value
Background: Menarche is a woman’s first menstruation and is determined by multiple factors. Objectives: The aim of this study was to evaluate the effect of exercise on the age of menarche in girls at ...guidance schools of Shiraz, Iran. Patients and Methods: This cross-sectional study attempted to evaluate the effect of regular exercise on the age of menarche in relation to demographic data in 483 randomly selected girls from guidance school in Shiraz, Iran, using statistical analysis including independent t-test, variance analysis and linear regression tests. Results: Participants were divided in two groups including 181 and 302 athletic and non-athletic girls, respectively. A statistically significant difference was found in the mean age at menarche between athletic 12.4 ± 0.9 SD and non-athletic girls 12.1 ± 1.0 SD years (P = 0.003). Linear regression indicated that mother’s age (P = 0.049) and exercise (P = 0.001) both have significant association with age at menarche. Conclusions: The age at menarche for the athletic group was significantly delayed. Exercise and mother’s age were significantly associated with age at menarche, whereas no significant association was observed between age at menarche and weight, BMI, father’s age, and mother’s and father’s educational levels.
As low back pain incidence is increasing, noninvasive modalities are gaining attention for their ability to achieve the best possible outcome with the least complications. Percutaneous laser disc ...decompression (PLDD) is currently popular for this purpose. This study aims to evaluate the effect of PLDD on disability and pain reduction in patients with lumbar disc herniation.
Thirty patients were enrolled in this study. Spinal nerve blocks were conducted by laser discectomy single stage injection of a needle into the disc space. The nucleus pulposus of herniated discs were irradiated with laser in order to vaporize a small part of the nucleus pulposus of the intervertebral discs and reduce the voluminosity of diseased discs. Patients were treated with 1000 J of 980 nm diode laser with 5 W energy. In order to measure the severity of pain, visual analog scale (VAS) and also ODI (Oswestry Disability Index) were used. Data were analyzed using SPSS version 12.
Thirty patients participated in this trial including 11 men and 19 women with a mean age (SD) of 40.8 (10.8) years. The mean patients VAS score and ODI level before and after discectomy showed statistically significant differences. The mean VAS and ODI scores showed no statistical difference between males and females (
<0.05) and percutaneous laser discectomy decreased the VAS and ODI at both groups of patients similarly.
We found the use of PLDD reduces pain and disability in patients as a noninvasive procedure.
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