The proximal tibiofibular joint (PTFJ) is easily ignored, although many diseases of the knee are caused by PTFJ injuries. Therefore, studying PTFJ biomechanics is very important. The effects of PTFJ ...injury on ankle function have been reported. However, few studies have assessed the effects of PTFJ injury on the knee joint. This study was performed to describe the biomechanical effects of PTFJ on the knee joint according to a three-dimensional finite element model.
The knee joint of a healthy volunteer was scanned by CT and MRI. CT and MRI scanning data in DICOM format were imported into Mimics software. Subsequently, 3D models of the normal and PTFJ injured knee, including the bone, cartilage, meniscus and ligament structures were established, and their validity was verified on the basis of available studies in literature. The biomechanical changes in the two knee models under different conditions were compared.
The validity of the intact model was verified. No significant difference was observed in tibial mobility in the two models under the conditions of 134 N forward, 10 N·m internal rotation and 10 N·m valgus load. After application of 134 N backward, 10 N·m varus and external rotation load with respect to the tibia, the posterior movement of the tibia and the varus and external rotation angles of the tibia were 3.583±0.892 mm, 4.799±0.092° and 18.963±0.027° in the normal knee model, and 5.127±1.224 mm, 5.277±0.104° and 21.399±0.031° in the PTFJ injury model, respectively, and a significant statistical difference was observed.
PTFJ played an important role in maintaining the posterolateral stability of the knee joint and thus deserves more attention in clinical operations.
The outbreak of goose gout disease caused by novel goose astrovirus type 1 (GAstV-1) has resulted in huge economic losses to the goose industry in China since 2017. However, little is known about the ...B cell epitopes in major antigen of GAstV-1 and the serological approach for detection of GAstV-1 is not available. In this study, three novel monoclonal antibodies (mAbs) against the ORF2 protein were first generated and designated as 3G6, 5H7, and 6C6, respectively. Epitope mapping revealed that mAb 3G6, 5H7, and 6C6 recognized
695
AVRFEKGGHE
704
,
685
EKALSAPQAG
694
, and
635
DDDPLSDVTS
644
in ORF2, respectively. Sequence alignments found that the three epitopes were highly conserved in GAstV-1 but not in other AAstV members. Moreover, a mAb-based sandwich ELISA for the detection of GAstV-1 was first developed using mAb 6C6. The sandwich ELISA only reacted with GAstV-1 but not with GAstV-2 and the other goose-associated viruses tested. The limit of the detection of the sandwich ELISA reaches 1.58 × 10
3
TCID
50
/mL of GAstV-1. Notably, mAb 6C6 could also efficiently react with the GAstV-1 in tissue frozen sections of the clinical infected goose through IFA. The mAbs generated in this study pave the way for further studying on the role of ORF2 in the infection and pathogenesis of GAstV, and the sandwich ELISA and the tissue frozen section-IFA approaches established here provide efficient and rapid serological diagnostic tools for detection of GAstV-1.
Key points
•
Three novel B cell epitopes were identified in ORF2 of GAstV-1.
•
mAb-based ELISA and IFA for detection of GAstV-1 were developed.
African swine fever (ASF) is one of the highly contagious and lethal diseases among domestic pigs and wild boars. The capsid protein P72 of African swine fever virus (ASFV) is very important for the ...diagnosis and vaccine development. However, the epitope of the protein is not clear. In this study, capsid protein P72 was expressed in Sf9 cells along with its chaperone B602L. A total of ten monoclonal antibodies (mAbs) specific to P72 protein were developed by fusions between SP2/0 cells and spleen cells of mice immunized with the recombinant-P72&B602L proteins expressed in Sf9 cells. Four linear B cell epitopes
31
SNIKNVNKSY
40
,
41
GKPDP
45
,
56
HLVHFNAH
63
and
185
ERLYE
189
were identified. Biological information analysis illustrated that epitopes
31
SNIKNVNKSY
40
,
41
GKPDP
45
and
185
ERLYE
189
were highly conserved within different ASFV strains. These findings may lead to a better understanding of the antibody-antigen interaction and provide new insights into the vaccine research and serological diagnosis of ASF.
Several kinds of one-dimensional TiO2 nanostructures including nanorod arrays, nanobelt arrays, and fan-shaped rectangular parallelepiped rods were prepared by alkali hydrothermal method. Their ...morphology and crystalline phase could be controlled by changing the concentration of alkali solution. They were used as photoanodes to assemble front-side illumination dye-sensitized solar cells, and the cell performance was correlated with the nanostructure. The highest conversion efficiency (6.0%) was achieved with highly ordered TiO2 nanorod arrays. We demonstrate that the oriented nanorods with appropriate lengths are beneficial to improve the electron transport property and thus lead to the increase of photocurrent, together enhancing the power conversion efficiency.
Abstract
Mie-resonant metasurfaces composed of subwavelength dielectric resonators enable an efficient route for electromagnetic wave manipulation. Among these manipulations, a localized mode with a ...high-quality factor coexisting with a continuous spectrum of radiating waves termed bound state in the continuum (BIC) can arouse many exotic applications in photonics. Here, we demonstrate the terahertz BIC in a dielectric membrane metasurface and analyze its resonant nature based on Mie-resonant multipoles and vector spherical harmonics. The intrinsic splitting of the resonances under oblique incidence is also explored, in which the conversion of multipole radiation patterns versus the oblique angle will drive the resonances from BIC to leaky modes or vice versa. Both Γ and off-Γ point BICs could be identified as the superposition cancellation of vector spherical harmonics for both p-wave and s-wave. Our research not only provides a novel perspective for exploring the essence of BIC metasurfaces in the terahertz regime, but also points new opportunities for achieving terahertz BIC metasurfaces with ultra-high quality factors.
Avian infectious bronchitis (IB) is a highly contagious disease caused by infectious bronchitis virus (IBV). Vaccination is an effective approach for controlling IBV. Therefore, reliable immune ...monitoring for IB is critical for poultry. In this study, a novel peptide derived from S2 protein was used to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of broadly cross-reactive antibodies against IBV. The peptide-based ELISA (pELISA) showed good specificity and sensitivity in detecting IBV antibodies against different serotypes. A semilogarithmic regression method for determining IBV antibody titers was also established. Antibody titers detected by pELISA and calculated with this equation were statistically similar to those evaluated by indirect fluorescence assay (IFA). Moreover, the comparison analysis showed a 96.07% compatibility between the pELISA and IDEXX ELISA. All these data demonstrate that the pELISA generated here can be as a rapid and reliable serological surveillance tool for monitoring IBV infection or vaccination.
Yield gap analysis could provide management suggestions to increase crop yields, while the understandings of resource utilization efficiency could help judge the rationality of the management. Based ...on more than 110 published papers and data from Food and Agriculture Organization (FAO, www.fao.org/faostat) and the Global Yield Gap and Water Productivity Atlas (www.yieldgap.org), this study summarized the concept, quantitative method of yield gap, yield-limiting factors, and resource utilization efficiency of the three major food crops (wheat, maize and rice). Currently, global potential yields of wheat, maize and rice were 7.7, 10.4 and 8.5 t ha–1, respectively. However, actual yields of wheat, maize and rice were just 4.1, 5.5 and 4.0 t ha–1, respectively. Climate, nutrients, moisture, crop varieties, planting dates, and socioeconomic conditions are the most mentioned yield-limiting factors. In terms of resource utilization, nitrogen utilization, water utilization, and radiation utilization efficiencies are still not optimal, and this review has summarized the main improvement measures. The current research focuses on quantitative potential yield and yield gap, with a rough explanation of yield-limiting factors. Subsequent research should use remote sensing data to improve the accuracy of the regional scale and use machine learning to quantify the role of yield-limiting factors in yield gaps and the impact of change crop management on resource utilization efficiency, so as to propose reasonable and effective measures to close yield gaps.
•A novel IBV variant CK/CH/2010/JT-1 was identified.•A novel genotypic cluster IBV has emerged in China.•Isolate CK/CH/2010/JT-1 originated from homologous RNA recombination.•Isolate CK/CH/2010/JT-1 ...is highly virulent.
The emergence of new infectious bronchitis virus (IBV) variants is often disastrous in the poultry industry. In this study, an IBV, CK/CH/2010/JT-1, was isolated from an H120- and 4/91-IBV-vaccinated flock in China. Antisera against vaccine strains H120 and 4/91 could not provide effective protection against CK/CH/2010/JT-1 in virus neutralization assays. CK/CH/2010/JT-1 could cause 43.75% mortality with respiratory and severe renal lesions in inoculated chickens. Phylogenetic analysis of the S1 gene showed that CK/CH/2010/JT-1 and 31 other isolates could be grouped as a new genotypic cluster. Recombination analysis revealed that three recombination events could be found in the genome of CK/CH/2010/JT-1 at positions 24709-365, 17160-19811 and 21136-21770. Whole-genome sequence analysis showed that CK/CH/2010/JT-1 originated from multiple template switches among QX-like, CK/CH/LSC/99I-, tl/CH/LDT3/03- and 4/91-type IBVs. All of these data demonstrated that CK/CH/2010/JT-1 is a new recombinant genotype IBV with high virulence. Our findings suggest that the surveillance of new genotype strains of IBV is very important for developing more effective anti-IBV strategies.
Tris(2,4-di-tert-butylphenyl) phosphite (AO168), an organophosphite antioxidant, can be oxidized to tris(2,4-di-tert-butylphenyl) phosphate (AO168 = O) during the production, processing, and ...application of plastics. AO168 = O can be further transformed to bis(2,4-di-tert-butylphenyl) phosphate and 2,4-di-tert-butylphenol. Here, we discovered the contamination of AO168 and its transformation products in dairy products for the first time. More samples contained AO168 (mean concentration: 8.78 ng/g wet weight ww), bis(2,4-di-tert-butylphenyl) phosphate (mean:11.1 ng/g ww) and 2,4-di-tert-butylphenol (mean: 46.8 ng/g ww) than AO168 = O (mean: 40.2 ng/g ww). The concentrations of AO168 and its transformation products were significantly correlated, and differed with the packaging material and storage conditions of the product. Estimated daily intakes (EDIs) of AO168 and its transformation products were calculated. Although the overall dietary risks were below one, transformation products accounted for 96.7% of the total hazard quotients. The high-exposure EDIs of total AO168 were above the threshold of toxicological concern (300 ng/kg bw/day), and deserve continual monitoring.
Display omitted
•AO168 and its transformation products were widespread in dairy products.•Significant correlations were observed among AO168 and its transformation products•Packaging material and storage conditions of the product mainly associated with the concentrations•Dietary risk of AO168 and its transformation products from dairy consumption is low
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