Ovarian cancer is currently the most life‑threatening type of gynecological malignancy with limited treatment options. Therefore, improved targeted therapies are required to combat ovarian cancer ...across the world. Sulforaphane is found in raw cruciferous vegetables. The chemotherapeutic and anti‑carcinogenic properties of sulforaphane have been demonstrated, however, the underlying mechanisms remain to be fully elucidated, particularly in ovarian cancer. In the present study, the possibility of repurposing sulforaphane as an anti‑ovarian cancer agent was examined. Cell viability and colony formation assay were used to test the anticancer efficiency of sulforaphane. Then wound healing assay, migration assay, cell cycle and apoptosis assays were used to detect how the drug worked on the cells. The mechanism of sulforaphane was investigated by western blot analysis. It was found that sulforaphane effectively suppressed the progression of human ovarian cancer cell proliferation, migration and cell cycle, and promoted apoptosis. Sulforaphane inhibited multiple cancer‑associated signaling pathways, including B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein, cytochrome c, Caspase‑3, phosphorylated AKT, phosphorylated nuclear factor‑κB, P53, P27, Cyclin‑D1 and cMyc, and reduced the expression levels of human epidermal growth factor receptor 2 in human ovarian cancer cells. Sulforaphane synergized with cisplatin to suppress the cancer cell proliferation and enhance ovarian cancer cell apoptosis. Xenograft experiments in vivo confirmed that sulforaphane effectively suppressed tumor growth by inhibiting ovarian cancer cell proliferation through targeting tumor‑related signals. The results indicated that sulforaphane may be repurposed as an effective anti‑ovarian cancer agent, with further preclinical or clinical investigations required.
There are three main surgical techniques to treat humeral shaft fractures: open reduction and plate fixation (ORPF), intramedullary nail (IMN) fixation, and minimally invasive percutaneous ...osteosynthesis (MIPO). We performed a network meta-analysis to compare three surgical procedures, including ORPF, IMN fixation, and MIPO, to provide the optimum treatment for humerus shaft fractures.
MEDLINE, EMBASE, Cochrane Bone, Joint and Muscle Trauma Group Specialised Register, and Cochrane library were researched for reports published up to May 2016. We only included randomized controlled trials (RCTs) comparing two or more of the three surgical procedures, including the ORPF, IMN, and MIPO techniques, for humeral shaft fractures in adults. The methodological quality was evaluated based on the Cochrane risk of bias tool. We used WinBUGS1.4 to conduct this Bayesian network meta-analysis. We used the odd ratios (ORs) with 95% confidence intervals (CIs) to calculate the dichotomous outcomes and analyzed the percentages of the surface under the cumulative ranking curve.
Seventeen eligible publications reporting 16 RCTs were included in this study. Eight hundred and thirty-two participants were randomized to receive one of three surgical procedures. The results showed that shoulder impingement occurred more commonly in the IMN group than with either ORPF (OR, 0.13; 95% CI, 0.03-0.37) or MIPO fixation (OR, 0.08; 95% CI, 0.00-0.69). Iatrogenic radial nerve injury occurred more commonly in the ORPF group than in the MIPO group (OR, 11.09; 95% CI, 1.80-124.20). There were no significant differences among the three procedures in nonunion, delayed union, and infection.
Compared with IMN and ORPF, MIPO technique is the preferred treatment method for humeral shaft fractures.
Gear mechanism transmits the motion and power of parallel axes, intersecting axes and staggered axes, which has been widely employed in production and life. Gear-type mobile mechanism is a type of ...robot that can achieve motion through gear transmission. Due to its unique motion mode, it can handle various tasks in certain unconventional environments, such as particularly steep surfaces. Cylindrical gear, bevel gear and non-cylindrical gear are taken as the main parts of the mechanism to form a novel research series, respectively. The models of gear-type mobile mechanism are established in this paper, and the degrees of freedom of the mechanism are briefly calculated based on the screw theory. Simultaneously, the influence of centroid trajectory on motion stability is discussed to solve the possible problem that opposite rotation occur during the movement. Furthermore, the trajectory model of zero moment point is calculated considering the motion of the gear-type mobile mechanism. After that, the simulation and experimental analysis of its motion capability show that the gear-type mobile mechanism has excellent characteristics in stability, flexibility and continuity.
Submerged fermentations of Monascus anka were performed with different nitrogen sources at different pH in 3 L bioreactors. The results revealed that the Monascus pigments dominated by different ...color components (yellow pigments, orange pigments or red pigments) could be selectively produced through pH control and nitrogen source selection. A large amount of intracellular pigments dominated by orange pigments and a small amount of water-soluble extracellular yellow pigments were produced at low pH (pH 2.5 and 4.0), independently of the nitrogen source employed. At higher pH (pH 6.5), the role of the nitrogen source became more significant. In particular, when ammonium sulfate was used as nitrogen source, the intracellular pigments were dominated by red pigments with a small amount of yellow pigments. Conversely, when peptone was used, intracellular pigments were dominated by yellow pigments with a few red pigments derivatives. Neither the presence of peptone nor ammonium sulfate promoted the production of intracellular orange pigments while extracellular pigments with an orangish red color were observed in both cases, with a higher yield when peptone was used. Two-stage pH control fermentation was then performed to improve desirable pigments yield and further investigate the effect of pH and nitrogen sources on pigments composition. These results provide a useful strategy to produce Monascus pigments with different composition and different color characteristics.
Multiple meta-analyses have been published to compare intramedullary nail and plate for treating humeral shaft fractures; however, results are discordant.The purposes of current study were to perform ...a systematic review of overlapping meta-analyses comparing intramedullary nail and plate fixation for the treatment of humeral shaft fractures, to appraise the methodological quality and the quality of reporting of meta-analyses, and to propose a guide through the currently discordant available evidence.This systematic review was performed according to the guidelines of Preferred Reporting Items for Systematic Reviews and Meta-analysis. The literature was systematically reviewed to identify meta-analyses comparing intramedullary nail and plate fixation for the treatment of humeral shaft fractures. Only meta-analyses exclusively including randomized clinical trials (RCTs) met eligibility criteria in this systematic review. Methodological quality for each included study was assessed using the Oxford Levels of Evidence and the Assessment of Multiple Systematic Reviews Instrument. We extracted all variables from the included studies and listed the results reported by them. Heterogeneity information of each variable was extracted for the included studies. An I of <60% is accepted in this systematic review. The Jadad algorithm was then applied to determine which meta-analyses provided the best evidence.Seven studies met the inclusion criteria in this study. All studies included RCTs or quasi-RCT and were Level II of evidence. Assessment of Multiple Systematic Reviews scores varied from 6 to 10 with a median of 7.86. Heterogeneity of each outcome was acceptable in those meta-analyses pooled results. The Jadad algorithm suggested that the meta-analyses can be selected based on the search strategies and application of selection. As a result, 2 meta-analyses with more RCTs were selected in this systematic review. The best available evidence suggested that the differences between intramedullary nail and plate fixation were not significant in fracture union, radial nerve injury, and infection. But intramedullary nail significantly increased the risk of shoulder complications (shoulder impingement and restriction of shoulder movement) and reoperation.We concluded that plate fixation is superior to intramedullary nail for the treatment of humeral shaft fractures.
Although transformational leadership (TL) is considered a kind of positive leadership, which can elevate followers in the long term, the mechanism of how TL influences employee well‐being remains a ...relatively untouched area. Based on survey data collected from 745 employees from the People's Republic of China (Beijing, n= 297; Hong Kong, n= 448), results revealed that employees' trust in the leader and self‐efficacy partially mediated the influence of TL on job satisfaction, and fully mediated the influence of TL on perceived work stress and stress symptoms. Implications of these findings for research and practice are discussed.
Bien que le leadership transformationnnel (TL) soit considéré comme une sorte de leadership positif qui peut faire progresser les suiveurs sur le long terme, le mécanisme par lequel TL contribue à leur bien être reste relativement inexploré. Les résultats basés sur des données collectées auprès d’un échantillon de 745 employés de la République Populaire de Chine (Beijin, n = 297; Hong Kong, n = 448), montrent que la confiance des suiveurs dans le leader et l’auto‐efficacité sont en partie dus à l’influence du TL sur la satisfaction au travail et sont entièrement dus à l’influence du TL sur le stress perçu au travail et les symptômes de stress. Les implications de ces résultats pour la recherche et la pratique sont discutées.
Extreme environments, for example high-salt-stress condition, that can induce secondary metabolite biosynthesis in fungi are a promising and effective strategy for producing natural
Monascus
pigments ...used as food colourants and nutraceutical supplements. In this study, the relationship between the mycelial morphology and expression of pigment biosynthetic genes in high-salt-stress fermentation (HSF) with
Monascus ruber
CGMCC 10910 was investigated. The
Monascus
fungus grew well under HSF conditions with 35 g/l NaCl, and the intracellular yellow pigment yield in HSF was 40% higher than that in conventional batch fermentation (CBF). Moreover, the mycelial morphology was maintained in a better state, with a hyphal diameter of 5–6 μm in HSF, indicating good biocatalytic activity for pigment synthesis. The rate of the relative content of intracellular orange pigments to yellow pigments (O/Y) significantly (
p
< 0.05) changed, and the extracellular yellow pigments were transformed into each other, indicating that the pigment biosynthesis pathway was changed to promote yellow pigment accumulation in HSF. The pigment biosynthesis genes
MpPKS5
,
MpFasB2
,
mppE
,
mppD
and
mppB
were significantly (
p
< 0.05) up-regulated by approximately 58.4–106.1%, whereas the regulatory genes
mppR1
and
mppR2
were significantly (
p
< 0.05) down-regulated by approximately 23.2% and 59.0% in HSF. Notably, the
mppE
gene was highly correlated with (
r
> 0.95,
p
< 0.05) hyphal diameter. These findings indicated that the cultivation of the
Monascus
fungus under high-salt-stress conditions was beneficial for pigment biosynthesis by controlling the mycelial morphology to regulate gene expression. This study first described the relationship between the mycelial morphology and expression of pigment biosynthetic genes in
Monascus
during fermentation.
Key Points
•
High-salt-stress fermentation (HSF) was first performed to improve Monascus pigment yield
.
•
Pigment biosynthesis was enhanced by maintaining the mycelial morphology in an improved state in HSF
.
•
Gene expression was up-/downregulated to promote yellow pigment accumulation in HSF
.
•
The mycelial morphology was highly related to the expression of pigment biosynthetic genes in HSF
.
Background COVID-19, as a significant public health issue, has had a major impact on the mental health of people worldwide. Research shows a significant positive correlation between individuals’ risk ...perception levels and negative emotions during the outbreak of COVID-19. However, some studies also suggest that the relationship between the two is not significant. Therefore, we will conduct a meta-analysis to explore the relationship between risk perception and negative emotions from cultural, temporal, and individual psychological perspectives. Methods Searches were conducted in the Web of Science, Pub Med, Google Scholar, PsycINFO, Scopus, and China National Knowledge Infrastructure databases, focusing on publications from January 2020 onwards, specifically targeting studies examining the relationship between risk perception and negative emotion during COVID-19. Results A total of 58 papers with 85 effect sizes were meta-analyzed using Comprehensive Meta-Analysis 3.0 software, with a combined sample of 83,948 individuals. Risk perception of COVID-19 showed a moderate positive correlation with negative emotions ( r = 0.211, 95%CI 0.18, 0.24). There was no moderating effect of tight-loose cultures on the relationship between risk perception of COVID-19 and negative emotions. However, the epidemic period, gender ratio, and measurement methods did have moderating effects on the relationship between risk perception of COVID-19 and negative emotions. Conclusion In future research, we can further develop theories related to the risk perception of COVID-19 and negative emotions, and based on these, formulate interventions to promote people’s mental health.
Oliver is widely distributed in China. This species has been used mainly in medicine due to the high concentration of chlorogenic acid (CGA), flavonoids, lignans, and other compounds in the leaves ...and barks. However, the categories of metabolites, dynamic changes in metabolite accumulation and overall molecular mechanisms involved in metabolite biosynthesis during
leaf growth and development remain unknown. Here, a total of 515 analytes, including 127 flavonoids, 46 organic acids, 44 amino acid derivatives, 9 phenolamides, and 16 vitamins, were identified from four
samples using ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) (for widely targeted metabolites). The accumulation of most flavonoids peaked in growing leaves, followed by old leaves. UPLC-MS analysis indicated that CGA accumulation increased steadily to a high concentration during leaf growth and development, and rutin showed a high accumulation level in leaf buds and growing leaves. Based on single-molecule long-read sequencing technology, 69,020 transcripts and 2880 novel loci were identified in
. Expression analysis indicated that isoforms in the flavonoid biosynthetic pathway and flavonoid metabolic pathway were highly expressed in growing leaves and old leaves. Co-expression network analysis suggested a potential direct link between the flavonoid and phenylpropanoid biosynthetic pathways via the regulation of transcription factors, including MYB (v-myb avian myeloblastosis viral oncogene homolog) and bHLH (basic/helix-loop-helix). Our study predicts dynamic metabolic models during leaf growth and development and will support further molecular biological studies of metabolite biosynthesis in
In addition, our results significantly improve the annotation of the
genome.
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•Medicinal values of guava leaves were upgraded through microbial co-fermentation.•Polyphenols from guava leaves was successfully identified.•α-Glucosidase inhibitory activities of ...guava leaves were promoted.•Methanol performed well in extracting bioactive components.
Although phenolic compounds linked to proteins or polysaccharides in the cell wall from herbal plant through β-glycoside bonds are difficult to extract in aqueous solution, microorganisms that produce some key enzymes may be useful for enhancing the release of phenolic compounds. Solid-state fermentation with a combination of different microorganisms was used to promote the bioactivities of the polyphenol component in GL. The results showed that co-fermentation of GL with Monascus anka GIM 3.592 and Saccharomyces cerevisiae GIM 2.139 led to a significant increase in contents of quercetin and total polyphenols. Including gallic acid, chlorogenic acid, rutin, isoquercitrin, avicularin, quercitrin, kaempferol, and quercetin, all polyphenols extracted from fermented guava leaves (FGL) were quantitatively identified by high-performance liquid chromatography time-of-flight electrospray ionization mass spectrometry (HPLC-TOF-ESI/MS). Quercetin, which presented the highest bioactivity, was significantly increased after microbial conversion. Total polyphenols extracted from FGL showed higher bioactivities in scavenging 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2-Azino-bis 3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS) caption radicals, anti-bacterial activity, and α-glucosidase inhibitory activity than those from GL. The activities of total polyphenols extracted from FGL by methanol were determined by calculating the IC50, with the highest values found to be 30.57μg/mL, 24.53μg/mL, and 0.09mg/mL for the scavenging of DPPH and ABTS+ radicals and inhibition of α-glucosidase, respectively. Methanol was more effective for extracting components with high α-glucosidase inhibitory activities than acetone. The methanol extract (0.20mg/mL) efficiently inhibited 92.05% of α-glucosidase activity, which was significantly higher than that obtained using acarbose solution at an equal concentration. This microbial co-fermentation method can be used to upgrade the nutritional or medicinal values of herb leaves efficiently.
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