To evaluate aerobic capacity, strength and other physiological, nutritional, and psychological variables which may influence the performance of transgender women (TW) athletes and compare them to ...cisgender women (CW) and cisgender men (CM) athletes, as well as changes in TW performance over the course of a year. Prospective cohort study including three groups: TW, CW and CM volleyball athletes. Subjects will be comprehensively assessed at two different moments: baseline and after 6-12 months of adequate hormonal therapy. Evaluation will comprise clinical, medical, nutritional and psychological interviews, incremental treadmill cardiopulmonary exercise testing, hand grip strength test, vertical jump test, analysis of sleep quality (Pittsburgh Sleep Quality Index), hormonal profile, echocardiogram, analysis of resting energy expenditure, assessment of bone mass and body composition through dual-energy X-ray absorptiometry scans, and untargeted metabolomic analysis. CW and CM matched by age, body mass index and level of physical activity will undergo a similar evaluation. The assessment of the strength, aerobic capacity, haematological, nutritional and psychological status of TW using gold-standard tests will contribute to understanding the impact of oestrogen therapy on the exercise performance of these athletes and how they compare with CW and CM.
Summary
Despite adequate immunization and penicillin prophylaxis, bacterial infections remain a leading cause of morbidity and mortality in patients with sickle cell disease (SCD). Besides ...hyposplenism, inflammatory and genetic factors might modulate their susceptibility to bacterial infections. We performed a candidate gene association of single nucleotide polymorphisms (SNPs) located in Toll‐like receptor (TLR) genes, encoding prominent molecules for innate immune responses, with the occurrence of bacterial infections in patients with SCD. A cohort followed in centres in Brazil, France and Senegal (n = 430) was divided in two groups: patients who presented at least one episode of bacterial infection (n = 235) and patients who never had bacterial infections (n = 195). There were no differences in gender or age distribution among the groups. The frequency of the TLR2 rs4696480 TA genotype was significantly lower in the infected group (50% vs. 67%, odds ratio OR = 0·50, 95% confidence interval CI 0·34–0·75, P < 0·001), and the TT genotype was significantly higher in the infected group (15% vs. 5%, OR = 3·18, 95% CI 1·53–6·61, P < 0·001). Previous reports demonstrated higher secretion of inflammatory factors in cells from AA individuals, lower occurrence and severity of immune diseases in T carriers. The rs4696480 TA genotype might stand between deleterious effects of over inflammatory response (AA genotype) and inefficient responses (TT genotype) to infectious agents in SCD settings.
Resumo Objetivou-se compreender as concepções e o processo de alta de crianças e adolescentes acompanhados em Centros de Atenção Psicossocial Infantojuvenis sob a perspectiva de terapeutas ...ocupacionais trabalhadores desses equipamentos. Foi realizada pesquisa de campo qualitativa, descritiva e exploratória, com 15 terapeutas ocupacionais que atuam em Centros de Atenção Psicossociais Infantojuvenis do município de São Paulo. Os dados foram coletados por meio de entrevista semiestruturada e analisados a partir da técnica do discurso do sujeito coletivo. A reflexão crítica sobre o conceito da alta na saúde mental infantojuvenil, o planejamento para a alta e a efetivação da articulação desses equipamentos com a rede intra e intersetorial durante todo o acompanhamento são determinantes para que o processo de alta de crianças e adolescentes que vivenciam o sofrimento psíquico aconteça de forma implicada, corresponsável e segura. O estudo avança ao pautar o tema da alta no contexto do cuidado em saúde mental infantojuvenil, revelando desafios e perspectivas para o aprimoramento de ações e de políticas públicas, e fortalecendo a Reforma Psiquiátrica brasileira.
ABSTRACT The objective was to understand the conceptions and process of discharging children and adolescents in Child Psychosocial Care Centers from the perspective of occupational therapists working in these locations. Qualitative, descriptive and exploratory field research was carried out with 15 occupational therapists who work at Child Psychosocial Care Centers in the city of São Paulo. Data were collected through a semi-structured interview and analyzed using the Collective Subject Discourse technique. Critical reflection on the concept of discharge in child and adolescent mental health, planning for discharge and the articulation of these services with the intra and intersectoral network throughout the follow-up are determinants for the discharge process of children and adolescents who experience psychological distress happen in an implicated, co-responsible and safe way. The study advances by addressing the issue of discharge in the context of child and adolescent mental health care, revealing challenges and perspectives for the improvement of actions and public policies and strengthening the Brazilian Psychiatric Reform.
Invertases are used for several purposes; one among these is the production of fructooligosaccharides. The aim of this study was to biochemically characterize invertase from industrial Saccharomyces ...cerevisiae CAT-1 and Rhodotorula mucilaginosa isolated from Cerrado soil. The optimum pH and temperature were 4.0 and 70 °C for Rhodotorula mucilaginosa invertase and 4.5 and 50 °C for Saccharomyces cerevisiae invertase. The pH and thermal stability from 3.0 to 10.5 and 75 °C for R. mucilaginosa invertase, respectively. The pH and thermal stability for S. cerevisiae CAT-1 invertase from 3.0 to 7.0, and 50 °C, respectively. Both enzymes showed good catalytic activity with 10% of ethanol in reaction mixture. The hydrolysis by invertases occurs predominantly when sucrose concentrations are ≤5%. On the other hand, the increase in the concentration of sucrose to levels above 10% results in the highest transferase activity, reaching about 13.3 g/L of nystose by S. cerevisiae invertase and 12.6 g/L by R. mucilaginosa invertase. The results demonstrate the high structural stability of the enzyme produced by R. mucilaginosa, which is an extremely interesting feature that would enable the application of this enzyme in industrial processes.
Harvestmen (Arachnida, Opiliones) are arachnids that rely on chemicals for communication and are particularly dependent on high humidity. The harvestman Jussara spec. (Sclerosomatidae) clearly ...prefers to rest on the stem and leaves of Psychotria suterella Muell. Arg. (Rubiaceae), a plant having a complex architecture of overlapping branches. So far, few studies have focused on understanding how harvestmen find their host plant and the benefits associated with the selected plant. Here, we investigated cues harvestmen may exploit to find P. suterella and the potential benefits for harvestmen of this interaction. To address how harvestmen find the plant, we tested two non‐exclusive hypotheses: harvestmen use chemicals from conspecifics, and/or harvestmen use chemicals from plants. For the first hypothesis, we assessed the number of harvestmen choosing P. suterella with or without chemicals of Jussara spec. on the main stem. We did not find evidence that Jussara spec. uses chemicals from conspecifics to select P. suterella, at least in isolation, without any mechanical stimuli such as stem texture or size. For the second hypothesis, we tested harvestman behavior exposed to volatiles from P. suterella, a non‐preferred host plant – Impatiens walleriana Hook.f. (Balsaminaceae) – and a blank treatment, both in a triangular arena and in a Y‐tube olfactometer. We also found no evidence that the harvestmen use plant volatiles to find it. We also tested two non‐exclusive hypotheses regarding the benefits of selecting P. suterella for harvestmen. The first hypothesis is that P. suterella offers a higher humidity than other plants in the micro‐environment surrounding the leaves because of a high leaf transpiration rate. Harvestmen could benefit from this because they rest with the body in contact with the leaf or at less than 5 mm from it. We did not find evidence that leaf transpiration rate is important for Jussara spec., as P. suterella did not present high rates compared to other local species. The second hypothesis is that the leaves of P. suterella provide a better shadow than other plants, acting as a sun shade due to its complex architecture with overlapping leaves. We measured light passage through the leaves of P. suterella and three other local species and found that less light passes through the leaves of P. suterella, which therefore provides darker shadow. This potentially provides a favorable micro‐climate for harvestmen, which might help to explain the micro‐habitat preference of Jussara spec.
The origin of the Cretaceous laminites of the Crato Formation, Araripe Basin, northeastern Brazil, has been intensely debated since the beginning of the last century. The monotonous, up to 10-m-thick ...succession composed of very fine fossiliferous laminites in the middle of the unit lacked diagnostic features for assigning a chemically or biologically induced origin for this facies. The presence of a highly diverse and very well preserved allochthonous to parautochthonous fossil assemblage, associated with scattered halite pseudomorphs throughout the succession, led many authors to believe that these limestones were chemically deposited in a highly stressful, evaporitic shallow-water environment, such as a hypersaline lake close to marine environments. Recently, a micro- and ultrastructural analysis of the laminites yielded structures undoubtedly associated with a biological origin. Several examples of lithified in situ preserved coccoid and filamentous cells and extracellular polymeric substances suggest that the deposition of the laminated limestones was, at some levels, strongly influenced by microbial activity. Here, we record various examples of stromatolite microbialites (mounds, domes, and pseudo-columns) found at distinct stratigraphic levels in the middle part of the Crato Formation. Macro-, meso-, and microscopic features confirm the biologically induced mineralization and the existence of metabolic activity of microbes during the formation of the laminites. Biomat growth may also have played a major role in the excellent preservation of fossils in this famous Cretaceous
Konservat
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Lagerstätte
from Brazil.
Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the ...proliferative processes. The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women. A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP). Profile determination and the concentration of hyaluronic acid was performed by the biochemical method of the fluorimetric assay (ELISA-like). Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting. Statistical analyses were performed with one-way ANOVA, followed by the Bonferroni test. Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase. We suggest that PCOS patients have different composition of hyaluronic acid in relation to a regular cycle in the proliferative phase.
In Brazil dengue has been a major public health problem since DENV-1 introduction and spread in 1986. After a low or silent co-circulation, DENV-1 re-emerged in 2009 causing a major epidemic in the ...country in 2010 and 2011. In this study, the phylogeny of DENV-1 strains isolated in RJ after its first introduction in 1986 and after its emergence in 2009 and 2010 was performed in order to document possible evolutionary patterns or introductions in a re-emergent virus.
The analysis of the E gene sequences demonstrated that DENV-1 isolated during 2009/2010 still belong to genotype V (Americas/Africa) but grouping in a distinct clade (lineage II) of that represented by earlier DENV-1 (lineage I). However, strains isolated in 2011 grouped together forming another distinct clade (lineage III).
The monitoring of DENV is important to observe the spread of potentially virulent strains as well to evaluate its impact over the population during an outbreak. Whether explosive epidemics reported in Brazil caused mainly by DENV-1 was due to lineage replacement, or due the population susceptibility to this serotype which has not circulated for almost a decade or even due to the occurrence of secondary infections in a hyperendemic country, is not clear. This is the first report of multiple lineages of DENV-1 detected in Brazil.
A twelve-year-old male Saint-Bernard dog weighing 46.0kg was referred to the pathology laboratory at the veterinary hospital for an autopsy. The patient had a history of apathy, hyporexia, ...polydipsia, increased rectal temperature, decreased body condition score, muffled cardiac auscultation, hypoglycemia and hepatomegaly. The dog developed cardiorespiratory arrest on the same day and was subsequently referred for post-mortem examination. In the necropsy, the macroscopic evaluation hepatopathy with the liver in the aspect of “nutmeg”, scrotal sac with abrasions, and the presence of a nodular lesion in the testes. Histopathology confirmed a diagnosis of testicular seminoma with liver metastasis. Testes are the third most common localization for dog tumors. However, metastasis of seminoma is uncommon. Thus, the case brings clinicopathological description of a rare case of testicular seminoma with liver metastasis in a Saint-Bernard.
Calcium is essential for plants, yet as its mobility is limited, the understanding of the rate of Ca2+ accumulation and deposition in tissues of tubers, as well as the interactions with other ...critical nutrients prompted this study. To assess the interactions and differential accumulation of micro and macronutrients in the tissues of tubers, Solanum tuberosum L. varieties Agria and Rossi were cultivated and, after the beginning of tuberization, four foliar sprayings (at 8–10 day intervals) with CaCl2 (3 and 6 kg ha−1) or Ca(NO3)2 (2 and 4 kg ha−1) solutions were performed. It was found that both fertilizers increased Ca accumulation in tubers (mostly in the parenchyma tissues located in the center of the equatorial region). The functioning of the photosynthetic apparatus was not affected until the 3rd application but was somewhat affected when approaching the end of the crop cycle (after the 4th application), although the lower dose of CaCl2 seemed to improve the photochemical use of energy, particularly when compared with the greater dose of Ca(NO3)2. Still, none of these impacts modified tuber height and diameter. Following the increased accumulation of Ca, in the tubers of both varieties, the mean contents of P, K, Na, Fe, and Zn revealed different accumulation patterns. Moreover, accumulation of K, Fe, Mn, and Zn prevailed in the epidermis, displaying a contrasting pattern relative to Ca. Therefore, Ca accumulation revealed a heterogeneous trend in the different regions analyzed, and Ca enrichment of tubers altered the accumulation of other nutrients.
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