The domain name system (DNS) that maps alphabetic names to numeric Internet Protocol (IP) addresses plays a foundational role in Internet communications. By default, DNS queries and responses are ...exchanged in unencrypted plaintext, and hence, can be read and/or hijacked by third parties. To protect user privacy, the networking community has proposed standard encryption technologies such as DNS over TLS (DoT), DNS over HTTPS (DoH), and DNS over QUIC (DoQ) for DNS communications, enabling clients to perform secure and private domain name lookups. We survey the DNS encryption literature published from 2016 to 2021, focusing on its current landscape and how it is misused by malware, and highlighting the existing techniques developed to make inferences from encrypted DNS traffic. First, we provide an overview of various standards developed in the space of DNS encryption and their adoption status, performance, benefits, and security issues. Second, we highlight ways that various malware families can exploit DNS encryption to their advantage for botnet communications and/or data exfiltration. Third, we discuss existing inference methods for profiling normal patterns and/or detecting malicious encrypted DNS traffic. Several directions are presented to motivate future research in enhancing the performance and security of DNS encryption.
Here we use the SCIREQ InExpose system to simulate a biologically relevant vaping model in mice to investigate the role of calcium signaling in vape-dependent pulmonary disease as well as to ...investigate if there is a gender-based difference of disease. Male and female mice were vaped with JUUL Menthol (3% nicotine) using the SCIREQ InExpose system for 2 weeks. Additionally, 2-APB, a known calcium signaling inhibitor, was administered as a prophylactic for lung disease and damage caused by vaping. After 2 weeks, mice were exposed to lipopolysaccharide (LPS) to mimic a bacterial infection. Post-infection (24 h), mice were sacrificed, and bronchoalveolar lavage fluid (BALF) and lungs were taken. Vaping primed the lungs for worsened disease burden after microbial challenge (LPS) for both males and females, though females presented increased neutrophilia and inflammatory cytokines post-vape compared to males, which was assessed by flow cytometry, and cytokine and histopathological analysis. This increased inflammatory burden was controlled by calcium signaling inhibition, suggesting that calcium dysregulation may play a role in lung injury caused by vaping in a gender-dependent manner.
Cyber-security risks for Internet of Things (IoT) devices sourced from a diversity of vendors and deployed in large numbers, are growing rapidly. Therefore, management of these devices is becoming ...increasingly important to network operators. Existing network monitoring technologies perform traffic analysis using specialized acceleration on network switches, or full inspection of packets in software, which can be complex, expensive, inflexible, and unscalable. In this paper, we use SDN paradigm combined with machine learning to leverage the benefits of programmable flow-based telemetry with flexible data-driven models to manage IoT devices based on their network activity. Our contributions are three-fold: (1) We analyze traffic traces of 17 real consumer IoT devices collected in our lab over a six-month period and identify a set of traffic flows (per-device) whose time-series attributes computed at multiple timescales (from a minute to an hour) characterize the network behavior of various IoT device types, and their operating states ( i.e., booting, actively interacted with user, or being idle); (2) We develop a multi-stage architecture of inference models that use flow-level attributes to automatically distinguish IoT devices from non-IoTs, classify individual types of IoT devices, and identify their states during normal operations. We train our models and validate their efficacy using real traffic traces; and (3) We quantify the trade-off between performance and cost of our solution, and demonstrate how our monitoring scheme can be used in operation for detecting behavioral changes (firmware upgrade or cyber attacks).
This paper investigates the opportunities and challenges in the use of dynamic radio transmit power control for prolonging the lifetime of body-wearable sensor devices used in continuous health ...monitoring. We first present extensive empirical evidence that the wireless link quality can change rapidly in body area networks, and a fixed transmit power results in either wasted energy (when the link is good) or low reliability (when the link is bad). We quantify the potential gains of dynamic power control in body-worn devices by benchmarking off-line the energy savings achievable for a given level of reliability.We then propose a class of schemes feasible for practical implementation that adapt transmit power in real-time based on feedback information from the receiver. We profile their performance against the offline benchmark, and provide guidelines on how the parameters can be tuned to achieve the desired trade-off between energy savings and reliability within the chosen operating environment. Finally, we implement and profile our scheme on a MicaZ mote based platform, and also report preliminary results from the ultra-low-power integrated healthcare monitoring platform we are developing at Toumaz Technology.
To increase engagement of historically underrepresented groups in health sciences research, we created the North Carolina Diversity and Inclusion Pathway Program (NC-DIPP). This year-long, paid ...internship provides undergraduate and graduate students from 2 historically Black colleges and universities an opportunity to gain real-world experience under the mentorship of expert faculty.
To evaluate the early experiences with the NC-DIPP program, we conducted semi-structured interviews with interns and program leaders. Faculty mentors completed an online questionnaire to describe their experiences to date. A thematic approach was used to analyze the findings.
In March-April 2023, 7 of 8 interns (88%), 6 of 11 mentors (54%), and 4 of 4 program leaders (100%) participated in various evaluation components. Overall, respondents agreed about the importance of programs like NC-DIPP, which further engage historically underrepresented groups in the health sciences. Interns had positive feedback about the internship, including real-world work experience, connections to experienced mentors, and early career planning. On a scale of 1 (poor) to 10 (excellent), interns rated their experience as a median of 8.3 (range: 4.5-10.0). Mentors had favorable but slightly lower scores (median: 7.0, range: 5.0-8.0). Areas for improvement were noted, including clearer expectations, improved logistical support, and central engagement of interns across projects.
This early evaluation of NC-DIPP was generally favorable across all stakeholder groups. By providing a long-term experience in health science research, such programs can contribute to work experience, career planning, and professional networking.
As electronic cigarette (E-cig) use, also known as "vaping", has rapidly increased in popularity, data regarding potential pathologic effects are recently emerging. Recent associations between vaping ...and lung pathology have led to an increased need to scrutinize E-cigs for adverse health impacts. Our previous work (and others) has associated vaping with Ca2+-dependent cytotoxicity in cultured human airway epithelial cells. Herein, we develop a vaped e-liquid pulmonary exposure mouse model to evaluate vaping effects in vivo. Using this model, we demonstrate lung pathology through the use of preclinical measures, that is, the lung wet: dry ratio and lung histology/H&E staining. Further, we demonstrate that acute vaping increases macrophage chemotaxis, which was ascertained using flow cytometry-based techniques, and inflammatory cytokine production, via Luminex analysis, through a Ca2+-dependent mechanism. This increase in macrophage activation appears to exacerbate pulmonary pathology resulting from microbial infection. Importantly, modulating Ca2+ signaling may present a therapeutic direction for treatment against vaping-associated pulmonary inflammation.
A growing number of inexpensive, publicly available, validated air quality monitors are currently generating granular and longitudinal data on air quality. The expansion of interconnected networks of ...these monitors providing open access to longitudinal data represents a valuable data source for health researchers, citizen scientists, and community members; however, the distribution of these data collection systems will determine the groups that will benefit from them. Expansion of these and other exposure measurement networks represents a unique opportunity to address persistent inequities across racial, ethnic, and class lines, if the distribution of these devices is equitable. We present a lean template for local implementation, centered on groups known to experience excess burden of pulmonary disease, leveraging five resources, (a) publicly available, inexpensive air quality monitors connected via Wi-Fi to a centralized system, (b) discharge data from a state hospital repository (c) the U.S. Census, (d) monitoring locations generously donated by community organizations and (e) NIH grant funds. We describe our novel approach to targeting air-quality mediated pulmonary health disparities, review logistical and analytic challenges encountered, and present preliminary data that aligns with a growing body of research: in a high-burden zip code in Durham North Carolina, the census tract with the highest proportions of African Americans experienced worse air quality than a majority European-American census tract in the same zip code. These results, while not appropriate for use in causal inference, demonstrate the potential of equitably distributed, interconnected air quality sensors.
The importance of intracellular calcium (Ca
) in regulating integral biological functions such as cell division, cell motility, autophagy, apoptosis and gene transcription through its capacity as a ...ubiquitous second messenger is clear ....
Inhalation of Yersinia pestis causes primary pneumonic plague, a highly lethal syndrome with mortality rates approaching 100%. Pneumonic plague progression is biphasic, with an initial ...pre-inflammatory phase facilitating bacterial growth in the absence of host inflammation, followed by a pro-inflammatory phase marked by extensive neutrophil influx, an inflammatory cytokine storm, and severe tissue destruction. Using a FRET-based probe to quantitate injection of effector proteins by the Y. pestis type III secretion system, we show that these bacteria target alveolar macrophages early during infection of mice, followed by a switch in host cell preference to neutrophils. We also demonstrate that neutrophil influx is unable to limit bacterial growth in the lung and is ultimately responsible for the severe inflammation during the lethal pro-inflammatory phase.
Though the current preponderance of evidence indicates the toxicity associated with the smoking of tobacco products through conventional means, less is known about the role of "vaping" in respiratory ...disease. "Vaping" is described as the use of electronic cigarettes (E-Cigarettes or E-Cigs), which has only more recently been available to the public (∼10 years) but has quickly emerged as a popular means of tobacco consumption worldwide. The World Health Organization (WHO) declared the SARS-CoV-2 outbreak as a global pandemic in March 2020. SARS-CoV-2 can easily be transmitted between people in close proximity through direct contact or respiratory droplets to develop coronavirus infectious disease 2019 (COVID-19). Symptoms of COVID-19 range from a mild flu-like illness with high fever to severe respiratory distress syndrome and death. The risk factors for increased disease severity remain unclear. Herein, we utilize a murine-tropic coronavirus (beta coronavirus) MHV-A59 along with a mouse model and measures of pathology (lung weight/dry ratios and histopathology) and inflammation (ELISAs and cytokine array panels) to examine whether vaping may exacerbate the pulmonary disease severity of coronavirus disease. While vaping alone did result in some noted pathology, mice exposed with intranasal vaped e-liquid suffered more severe mortality due to pulmonary inflammation than controls when exposed to coronavirus infection. Our data suggest a role for vaping in increased coronavirus pulmonary disease in a mouse model. Furthermore, our data indicate that disease exacerbation may involve calcium (Ca
) dysregulation, identifying a potential therapeutic intervention.
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