Oxidative stress has been demonstrated to play a pivotal role in the pathological processes of many neurodegenerative diseases. In the present study, we demonstrated that Chrysanthemum boreale Makino ...extract (CBME) suppresses oxidative stress-induced neurotoxicity in human neuroblastoma SH-SY5Y cells and elucidated the underlying molecular mechanism. Our observations revealed that CBME effectively protected neuronal cells against H2O2-induced cell death by preventing caspase-3 activation, Bax upregulation, Bcl-2 downregulation, activation of three mitogen-activated protein kinases (MAPKs), cAMP response element-binding protein (CREB) and NF-κB phosphorylation, and iNOS induction. These results provide evidence that CBME has remarkable neuroprotective properties in SH-SY5Y cells against oxidative damage, suggesting that the complementary or even alternative role of CBME in preventing and treating neurodegenerative diseases is worth further studies.
Tg2576 transgenic mice for Alzheimer's disease (AD) exhibited significant phenotypes for neuropathological constipation, but no research has been conducted on the association of the fecal microbiota ...with dysbiosis. The correlation between fecal microbiota composition and neuropathological constipation in Tg2576 mice was investigated by examining the profile of fecal microbiota and fecal microbiota transplantation (FMT) in 9-10-month-old Tg2576 mice with the AD phenotypes and constipation. Several constipation phenotypes, including stool parameters, colon length, and histopathological structures, were observed prominently in Tg2576 mice compared to the wild-type (WT) mice. The fecal microbiota of Tg2576 mice showed decreases in Bacteroidetes and increases in the Firmicutes and Proteobacteria populations at the phylum level. The FMT study showed that stool parameters, including weight, water content, and morphology, decreased remarkably in the FMT group transplanted with a fecal suspension of Tg2576 mice (TgFMT) compared to the FMT group transplanted with a fecal suspension of WT mice (WFMT). The distribution of myenteric neurons and the interstitial cells of Cajal (ICC), as well as the enteric nervous system (ENS) function, remained lower in the TgFMT group. These results suggest that the neuropathological constipation phenotypes of Tg2576 mice may be tightly linked to the dysbiosis of the fecal microbiota.
Biomineralization, a well-known natural phenomenon associated with various microbial species, is being studied to protect and strengthen building materials such as concrete. We characterized ...Rhodococcus erythreus S26, a novel urease-producing bacterium exhibiting CaCO3-forming activity, and investigated its ability in repairing concrete cracks for the development of environment-friendly sealants. Strain S26 grown in solid medium formed spherical and polygonal CaCO3 crystals. The S26 cells grown in a urea-containing liquid medium caused culture fluid alkalinization and increased CaCO3 levels, indicating that ureolysis was responsible for CaCO3 formation. Urease activity and CaCO3 formation increased with incubation time, reaching a maximum of 2054 U/min/mL and 3.83 g/L, respectively, at day four. The maximum CaCO3 formation was achieved when calcium lactate was used as the calcium source, followed by calcium gluconate. Although cell growth was observed after the induction period at pH 10.5, strain S26 could grow at a wide range of pH 4–10.5, showing its high alkali tolerance. FESEM showed rhombohedral crystals of 20–60 µm in size. EDX analysis indicated the presence of calcium, carbon, and oxygen in the crystals. XRD confirmed these crystals as CaCO3 containing calcite and vaterite. Furthermore, R. erythreus S26 successfully repaired the artificially induced large cracks of 0.4–0.6 mm width.
Polymicrobial wound infections are a major cause of infectious disease-related morbidity and mortality worldwide. In this study, we prepared a nitric oxide (NO)-releasing oxidized bacterial ...cellulose/chitosan (BC
/CHI) crosslinked hydrogel to effectively treat polymicrobial wound infections. Linear polyethyleneimine diazeniumdiolate (PEI/NO) was used as the NO donor. The aldehyde group of BC
and the amine of CHI were used as crosslinked hydrogel-based materials; their high NO loading capacity and antibacterial activity on the treatment of polymicrobial-infected wounds were investigated. The blank and NO-loaded crosslinked hydrogels, namely BC
-CHI and BC
-CHI-PEI/NO, were characterized according to their morphologies, chemical properties, and drug loading. BC
-CHI-PEI/NO exhibited sustained drug release over four days. The high NO loading of BC
-CHI-PEI/NO enhanced the bactericidal efficacy against multiple bacteria compared with BC
-CHI. Furthermore, compared with blank hydrogels, BC
-CHI-PEI/NO has a favorable rheological property due to the addition of a polymer-based NO donor. Moreover, BC
-CHI-PEI/NO significantly accelerated wound healing and re-epithelialization in a mouse model of polymicrobial-infected wounds. We also found that both crosslinked hydrogels were nontoxic to healthy mammalian fibroblast cells. Therefore, our data suggest that the BC
-CHI-PEI/NO developed in this study improves the efficacy of NO in the treatment of polymicrobial wound infections.
This paper presents the VCHP platform as a solution to address PV curtailment and line congestion in scenarios of increasing renewable energy penetration. Solar PV generation profiles and load ...profiles were generated for three scenarios (2025, 2030, and 2035) using data provided by KPX. Modifications were made to the IEEE 30 Bus model to accurately reflect the Korean power system, including the introduction of PCA and LCA at relevant buses. Line congestion was evaluated using metrics such as TUR, STUR, and TLR. The research findings indicate that integrating the VCHP platform in all scenarios effectively alleviates line congestion, as shown by the TUR remaining below 1. Importantly, the reduction in line losses exceeds the decrease in power flow, demonstrating the effectiveness of VCHP in reducing power losses. The results suggest that as renewable energy sources increase, line congestion issues may arise in the existing power system. However, integrating the proposed VCHP platform is a valuable solution for effectively utilizing surplus PV energy and improving the stability of the power grid. The adoption of such a platform can significantly enhance the operation and management of the power system.
The purpose of this study was to evaluate the structural performance of composite deck slabs containing macro-synthetic fibers. after a fire by proposing a deflection estimation method for ...non-fireproof structural decks. Therefore, this study evaluated the fire resistance performance and deflection of deck slabs mixed with macro-synthetic fibers. Afterward, the deflection estimation method considering the thermal characteristics of concrete and deck plates was proposed. A material test was first conducted to evaluate the mechanical properties of concrete mixed with macro-synthetic fibers. This test found that the compressive strength and elasticity modulus of concrete mixed with macro-synthetic fibers was greater than that of general concrete. A flexural tensile test confirmed that residual strength was maintained after the maximum strength was achieved. The fire resistance of the deck slab was adequate even when a fire-resistant coating was not applied. The internal temperature was lowest for the specimen with macro-synthetic fibers. Deflection was evaluated using previously published equations and standards. The deflection evaluation confirmed that the temperature distribution should be applied differently in the estimation method that uses the thermal load of the deck slab.
Biocalcification is a natural biochemical process, which has been regarded as a promising method for sequestering heavy metals or carbon dioxide in the environment, healing cracks in concrete ...structures, and stabilizing soil. One of the key factors in this process is calcium carbonate-producing bacteria. The purpose of this study was to maximize the production of calcium carbonate by alkaliphilic Bacillus psychrodurans LC40 isolated from a limestone cave, by manipulating the medium composition for fast and non-detrimental crack healing, and to investigate the mechanism of its production. Strain LC40 could grow well in the strongly alkaline region (pH 9.5–11), indicating its alkaliphilic nature. The optimal medium for calcium carbonate production contained 2% tryptone, 1.5% urea, 0.15% NaHCO3, and 150 mM calcium formate (pH 6). Using this medium, the yield of calcium carbonate at 72 h was approximately 8.6-fold higher than that obtained through Urea-CaCl2 medium. In this culture, the urease and carbonic anhydrase activities were observed simultaneously, and the pH of the medium was found to have increased to 9.4, leading to maximum calcium carbonate production. This suggests that this pH value is achieved by the synergistic action of the two enzymes, resulting in a high calcium carbonate yield. The crystals were characterized by FESEM, EDS and XRD, which confirmed the production of rhombohedral and spherical calcium carbonate crystals containing vaterite and calcite. Strain LC40 completely healed a 0.75 mm wide crack in a very short time of 3 days using the optimized medium as a cementation solution. Our findings indicate that B. psychrodurans LC40 could be a promising candidate for the development of eco-friendly biosealant applicable to environmentally stressed concrete structures.
Display omitted
•Novel alkaliphilic B. psychrodurans LC40 was characterized.•CaCO3 production by strain LC40 was optimized.•Strain LC40 produced both urease and carbonic anhydrase, and CaCO3 was produced by their synergy.•Strain LC40 was able to heal relatively large cracks on concrete in a short time.
The marine bacterium,
Bacillus
sp. SY-1, produced algicidal compounds that are notably active against the bloom-forming alga
Cochlodinium polykrikoides
. We isolated three algicidal compounds and ...identified these as mycosubtilins with molecular weights of 1056, 1070, and 1084 (designated MS 1056, 1070, and 1084, respectively), based on amino acid analyses and
1
H,
13
C, and two-dimensional nuclear magnetic resonance spectroscopy, including
1
H-
15
N heteronuclear multiple bond correlation analysis. MS 1056 contains a β-amino acid residue with an alkyl side chain of C
15
, which has not previously been seen in known mycosubtilin families. MS 1056, 1070, and 1084 showed algicidal activities against
C. polykrikoides
with 6-h LC
50
values of 2.3 ± 0.4, 0.8 ± 0.2, and 0.6 ± 0.1 μg/ml, respectively. These compounds also showed significant algicidal activities against other harmful algal bloom species. In contrast, MS 1084 showed no significant growth inhibitory effects against other organisms, including bacteria and microalgae, although does inhibit the growth of some fungi and yeasts. These observations imply that the algicidal bacterium
Bacillus
sp. SY-1 and its algicidal compounds could play an important role in regulating the onset and development of harmful algal blooms in natural environments.
Poultry feathers are widely discarded as waste worldwide and are considered an environmental pollutant and a reservoir of pathogenic bacteria. Therefore, developing sustainable and environmentally ...friendly methods for managing feather waste is one of the important environmental protection requirements. In this study, we investigated a rapid and eco–friendly method for the degradation and valorization of feather waste using keratinase–producing Pseudomonas geniculata H10, and evaluated the applicability of keratinase in environmentally hazardous chemical processes. Strain H10 completely degraded chicken feathers within 48 h by producing keratinase using them as sources of carbon, nitrogen, and sulfur. The culture contained a total of 402.8 μM amino acids, including 8 essential amino acids, which was higher than the chemical treatment. Keratinase was a serine-type metalloprotease with optimal temperature and pH of 30 °C and 9, respectively, and showed relatively high stability at 10–40 °C and pH 3–10. Keratinase was also able to degrade various insoluble keratins such as duck feathers, wool, human hair, and nails. Furthermore, keratinase exhibited more efficient depilation and wool modification than chemical treatment, as well as novel functionalities such as nematicidal and exfoliating activities. This suggests that strain H10 is a promising candidate for the efficient degradation and valorization of feather waste, as well as the improvement of current industrial processes that use hazardous chemicals.
Display omitted
•P. geniculata H10 completely degraded the feather waste in a short time of 48 h.•Valorizing low-value feather waste was achieved by strain H10.•Keratinase from strain H10 was able to degrade various recalcitrant keratin wastes.•The keratinase exhibited depilating, nematicidal and exfoliating activities.•They are promising biological tools for improving environment-harmful processes.
Cracking and erosion are critical factors that reduce the mechanical properties and stability of concrete structures and soil, respectively. They are recognized worldwide as severe disasters causing ...the collapse of many structures including stone heritage and dams, and landslides. Therefore, it is essential to propose effective and environment-friendly management methods to prevent them. Carbonatogenesis has recently received considerable attention as a reliable biological process for remediating cracks in calcareous structures, stabilizing loose soils, and sequestering CO2 in the environment. Isolating and characterizing carbonatogenic bacteria with excellent performance is crucial for applying this process to the field of environmental and civil engineering. The aim of this study was to isolate new CaCO3–precipitating bacteria and investigate various properties for their use as bioconsolidants. Furthermore, the possibility of restoring damaged structures and stabilizing loose sandy soil using isolated strain was investigated. Strain LC13 with urease and CaCO3–precipitating activity was isolated from limestone cave soil in Korea and identified as Arthrobacter sulfureus by phenotypic characterization and 16S rRNA gene analysis. Although cell growth was observed after an adaptation period at pH 11, strain LC13 grew well at pH 7–11, indicating alkali tolerance. The optimal conditions for CaCO3 precipitation were 1.0% yeast extract, 2.5% urea, 0.35% NaHCO3, and 400 mM CaCl2, with an initial pH of 6.5 at 30 °C. Under optimized conditions, maximal CaCO3 (22.92 ± 0.14 g/l) precipitated after 3 days, which was 10.8–fold higher than the value in a urea–CaCl2 medium. CaCO3 precipitation by strain LC13 was associated with an increased pH due to ureolysis and protein deamination. Using an optimized medium as a cementation solution, strain LC13 completely remediated 340–760 μm wide cracks over 3 days, and also restored the spalling of concrete surfaces. Furthermore, the sand treated with LC13 solidified with a surface strength of 14.9 kPa. Instrumental analysis confirmed that the crystals precipitated were a mixture of CaCO3 polymorphs composed of rhombohedral calcite and spherical vaterite. These results suggest that A. sulfureus LC13 may be useful for implementing sustainable biorestoration and environmental management technologies such as the in situ remediation of structural cracks and in situ prevention of soil erosion.
Display omitted
•Novel alkali-tolerant A. sulfureus LC13 that can precipitate CaCO3 was isolated.•Environmental parameters for CaCO3 precipitation were optimized.•Role of ureolysis and protein deamination on CaCO3 precipitation was determined.•Strain LC13 restored cracks and surface spalling in concrete over 3 days.•Strain LC13 was able to solidify sandy soil.
PDF
