Alien hand syndrome and dystonia in a pediatric patient Soman, Teesta; Steeves, Thomas D L; Lang, Anthony E
Movement disorders : official journal of the Movement Disorder Society,
07/2009, Letnik:
24, Številka:
10
Report
While microplastics have been recently detected in human blood and the placenta, their impact on human health is not well understood. Using a mouse model of environmental exposure during pregnancy, ...our group has previously reported that exposure to polystyrene micro- and nanoplastics throughout gestation results in fetal growth restriction. While polystyrene is environmentally relevant, polyethylene is the most widely produced plastic and amongst the most commonly detected microplastic in drinking water and human blood. In this study, we investigated the effect of maternal exposure to polyethylene micro- and nanoplastics on fetal growth and placental function. Healthy, pregnant CD-1 dams were divided into three groups: 10
ng/L of 740-4990 nm polyethylene with surfactant in drinking water (n = 12), surfactant alone in drinking water (n = 12) or regular filtered drinking water (n = 11). At embryonic day 17.5, high-frequency ultrasound was used to investigate the placental and fetal hemodynamic responses following exposure. While maternal exposure to polyethylene did not impact fetal growth, there was a significant effect on placental function with a 43% increase in umbilical artery blood flow in the polyethylene group compared to controls (p < 0.01). These results suggest polyethylene has the potential to cause adverse pregnancy outcomes through abnormal placental function.
Mosquitoes are important vectors for human and animal diseases. Genetic markers, like the mitochondrial COI gene, can facilitate the taxonomic classification of disease vectors, vector-borne disease ...surveillance, and prevention. Within the control region (CR) of the mitochondrial genome, there exists a highly variable and poorly studied non-coding AT-rich area that contains the origin of replication. Although the CR hypervariable region has been used for species differentiation of some animals, few studies have investigated the mosquito CR. In this study, we analyze the mosquito mitogenome CR sequences from 125 species and 17 genera. We discovered four conserved motifs located 80 to 230 bp upstream of the 12S rRNA gene. Two of these motifs were found within all 392 Anopheles (An.) CR sequences while the other two motifs were identified in all 37 Culex (Cx.) CR sequences. However, only 3 of the 304 non-Culicidae Dipteran mitogenome CR sequences contained these motifs. Interestingly, the short motif found in all 37 Culex sequences had poly-A and poly-T stretch of similar length that is predicted to form a stable hairpin. We show that supervised learning using the frequency chaos game representation of the CR can be used to differentiate mosquito genera from their dipteran relatives.
Globally, the production of marine bivalves has been steadily increasing over the past several decades. As the effects of human population growth are magnified, bivalves help provide food security as ...a source of inexpensive protein. However, as climate change alters sea surface temperatures (SST), the physiology, and thus the survival, growth, and distribution of bivalves are being altered. Challenges with managing bivalves may become more pronounced, as the uncertainty associated with climate change makes it difficult to predict future production levels. Modelling techniques, applied to both climate change and bivalve bioenergetics, can be used to predict and explore the impacts of changing ocean temperatures on bivalve physiology, and concomitantly on aquaculture production. This study coupled a previously established high resolution climate model and two dynamic energy budget models to explore the future growth and distribution of two economically and ecologically important species, the eastern oyster (Crassotrea virginica), and the blue mussel (Mytilus edulis) along the Atlantic coast of Canada. SST was extracted from the climate model and used as a forcing variable in the bioenergetic models. This approach was applied across three discreet time periods: the past (1986-1990), the present (2016-2020), and the future (2046-2050), thus permitting a comparison of bivalve performance under different temporal scenarios. Results show that the future growth is variable both spatially and interspecifically. Modelling outcomes suggest that warming ocean temperatures will cause an increase in growth rates of both species as a result of their ectothermic nature. However, as the thermal tolerance of C. virginica is higher than M. edulis, oysters will generally outperform mussels. The predicted effects of temperature on bivalve physiology also provided insight into vulnerabilities (e.g. mortality) under future SST scenarios. Such information is useful for adapting future management strategies for both farmed and wild shellfish. Although this study focused on a geographically specific area, the approach of coupling bioenergetic and climate models is valid for species and environments across the globe.
Poly- and perfluoroalkyl substances (PFAS) are a group of compounds with uses in industry and many consumer products. Concerns about the potential health effects of these compounds resulted in ...regulation by the Stockholm Convention on the use of three of the most common PFAS, including perfluorooctanoic acid (PFOA). Thousands of PFAS remain in production that are unregulated and for which their toxicity is unknown. Our group recently identified a new class of PFAS, fluorotelomer ethoxylates (FTEOs), in indoor dust and industrial wastewater. In this study, we investigated the effect of PFAS on placental metabolism by exposing healthy, pregnant CD-1 mice to PFOA or FTEOs at one of three concentrations (0 ng/L (controls), 5 ng/L, 100 ng/L) (n = 7–8/group). While PFOA is banned and PFOA concentrations in human blood are decreasing, we hypothesize that FTEOs will cause adverse pregnancy outcomes similar to PFOA, the compounds they were meant to replace. Placental tissue samples were collected at embryonic day 17.5 and 1H solid-state magic angle spinning nuclear magnetic resonance spectroscopy was used to determine the relative concentration of placental metabolites (n = 18–20/group). At the highest concentration, the relative concentrations of glucose and threonine were increased and the relative concentration of creatine was decreased in the PFOA-exposed placentas compared to controls (p < 0.05). In contrast, the relative concentrations of asparagine and lysine were decreased and the relative concentration of creatine was increased in the FTEOs-exposed placentas compared to controls (p < 0.05). Partial least squares - discriminant analysis showed the FTEOs-exposed and control groups were significantly separated (p < 0.005) and pathway analysis found four biochemical pathways were perturbed following PFOA exposure, while one pathway was altered following FTEOs exposure. Maternal exposure to PFOA and FTEOs had a significant impact on the placental metabolome, with the effect depending on the pollutant. This work motivates further studies to determine exposure levels and evaluate associations with adverse outcomes in human pregnancies.
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•Fluorotelomer ethoxylates (FTEOs) were previously found to be environmentally persistent.•The health impacts of FTEOs are unknown.•Exposure of pregnant mice to FTEOs results in abnormal placental metabolism.
Shallow coastal environments are becoming more susceptible to marine heatwaves, particularly in Atlantic Canada, which is experiencing higher rates of ocean warming compared with the global average. ...Understanding bivalves’ tolerance to heatwaves in Atlantic Canada is especially important, given the magnitude of bivalve aquaculture in this region. In this study, the physiological state of four commercially important bivalve species—blue mussel ( Mytilus edulis Linnaeus, 1758), eastern oyster ( Crassostrea virginica (Gmelin, 1791)), soft shell clam ( Mya arenaria Linnaeus, 1758), and hard clam ( Mercenaria mercenaria (Linnaeus, 1758))—was observed during a naturally occurring heatwave in Prince Edward Island, Canada. The metabolism, feeding physiology, and scope for growth of each species were compared. Results from this study, and comparisons with the literature, show interspecific variability in the metabolic rate and feeding physiology between species, but generally oysters, soft shell clams, and hard clams were less impacted during the heatwave compared with the blue mussels. Additionally, only the scope for growth of mussels declined throughout this experiment, which may be explained by cumulative heat stress, as temperatures experienced during this heatwave exceeded the thermal tolerance limits of mussels.
Macroalgal blooms in eutrophic coastal waters around the globe constitute a rising issue for ecosystems and economic activities. Sometimes leading to anoxic events, a better understanding of its ...growth dynamics is necessary to develop mitigation strategies and inform policies on nutrient runoff management. The development of a Dynamic Energy Budget (DEB) model for the sea lettuce, Ulva lactuca, provides a generic mechanistic description of energy and matter fluxes within the macroalgae and between macroalgae and the environment. Forcing variables consist of seawater temperature, light intensity, and seawater concentrations of dissolved carbon and nitrogen. The model includes a self-shading module for light reduction under heavy biomass and simulation outputs consist of (but are not limited to) total algae biomass, nutrient uptake (carbon and nitrogen), photosynthetic rate, and state of nutrient reserve. Model parameters were estimated using data-sets from the literature and laboratory experiments, then validated using an independent field study in two estuaries with contrasting nutrient loads that feed into Malpeque Bay, PEI (Canada). The validation step yielded accurate temporal predictions of sea lettuce biomass in both of these estuaries. These results indicate that the present mechanistic modelling approach for predicting sea lettuce dynamics captures salient patterns along a spectrum of nutrient loading and could therefore be of use for managing across diverse ecological conditions, which is particularly relevant for a widespread species like Ulva lactuca.
Ptr ToxA (ToxA) is a proteinaceous necrotizing host-selective toxin produced by Pyrenophora tritici-repentis, a fungal pathogen of wheat (Triticum aestivum). In this study, we have found that ...treatment of ToxA-sensitive wheat leaves with ToxA leads to a light-dependent accumulation of reactive oxygen species (ROS) that correlates with the onset of necrosis. Furthermore, the accumulation of ROS and necrosis could be inhibited by the antioxidant N-acetyl cysteine, providing further evidence that ROS production is required for necrosis. Microscopic evaluation of ToxA-treated whole-leaf tissue indicated that ROS accumulation occurs in the chloroplasts. Analysis of total protein extracts from ToxA-treated leaves showed a light-dependent reduction of the chloroplast protein RuBisCo. In addition, Blue native-gel electrophoresis followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis revealed that ToxA induces changes in photosystem I (PSI) and photosystem II (PSII) in the absence of light, and therefore, the absence of ROS. When ToxA-treated leaves were exposed to light, all proteins in both PSI and PSII were extremely reduced. We propose that ToxA induces alterations in PSI and PSII affecting photosynthetic electron transport, which subsequently leads to ROS accumulation and cell death when plants are exposed to light.
Several reviews in the past decade have heralded the benefits of embracing high‐throughput sequencing technologies to inform conservation policy and the management of threatened species, but few have ...offered practical advice on how to expedite the transition from conservation genetics to conservation genomics. Here, we argue that an effective and efficient way to navigate this transition is to capitalize on emerging synergies between conservation genetics and primary industry (e.g., agriculture, fisheries, forestry and horticulture). Here, we demonstrate how building strong relationships between conservation geneticists and primary industry scientists is leading to mutually‐beneficial outcomes for both disciplines. Based on our collective experience as collaborative New Zealand‐based scientists, we also provide insight for forging these cross‐sector relationships.
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