Genome-wide platforms for high-throughput profiling of circulating miRNA (oligoarray or miR-Seq) offer enormous promise for agnostic discovery of circulating miRNA biomarkers as a pathway for ...development in breast cancer detection. By harmonizing data from 15 previous reports, we found widespread inconsistencies across prior studies. Whether this arises from differences in study design, such as sample source or profiling platform, is unclear. As a reproducibility experiment, we generated a genome-wide plasma miRNA dataset using the Illumina oligoarray and compared this to a publically available dataset generated using an identical sample size, substrate and profiling platform. Samples from 20 breast cancer patients, 20 mammography-screened controls, as well as 20 breast cancer patients after surgical resection and 10 female lung or colorectal cancer patients were included. After filtering for miRNAs derived from blood cells, and for low abundance miRNAs (non-detectable in over 10% of samples), a set of 522 plasma miRNAs remained, of which 46 were found to be differentially expressed between breast cancer patients and healthy controls (p<0.05), of which only 3 normalized to baseline levels in post-resection cases and were unique to breast cancer vs. lung or colorectal cancer (miR-708*, miR-92b* and miR-568, none previously reported). We were unable to demonstrate reproducibility by various measures between the two datasets. This finding, along with widespread inconsistencies across prior studies, highlight the need for better understanding of factors influencing circulating miRNA levels as prerequisites to progress in this area of translational research.
In 2016, Lemonade was lauded as “Black girl magic” for the ways the hour-long HBO special (and subsequent album) celebrated Black women and the Southern gothic tradition. It also was the first hint ...of Beyoncé paying homage to West African Yoruba traditions. At the 2017 Grammys, her performance was both an invocation of the sacred in Western art history and further homage to Yoruba. The performance opened with poetry by Warsan Shire, and snapshots of her daughter, Blue Ivy, but the highlight was Beyoncé’s gold gown, and crown, and gold accessories, all of which symbolized the African goddess Osun. Released just before her Grammys performance, the I Have Three Hearts photo-series circulated as pregnancy images (she was pregnant with twins), but it also functioned as a repository of Beyoncé’s invocation of the sacred in Western culture, as embodied in Venus, and the African goddess, often labelled as “Black Venus.” This article is an examination of three images in the I Have Three Hearts series, taken by Awol Erizku, and the series’ accompanying poetry by Shire. I argue that it raises important questions about the role of visual culture in fashion and popular culture. Is Beyoncé the Venus of the twenty-first century? Does this photographic series remap Western visual culture to reimagine Black womanhood in the discourse on sexuality? Or, it is an example of pastiche in postmodern culture wherein truncated information is authorized, making everyone an expert without the demand for historical context?
Late-stage breast cancer metastasis is driven by dysregulated TGF-β signaling, but the underlying molecular mechanisms have not been fully elucidated. We attempted to recapitulate tumor and ...metastatic microenvironments via the use of biomechanically compliant or rigid 3D organotypic cultures and combined them with global microRNA (miR) profiling analyses to identify miRs that were upregulated in metastatic breast cancer cells by TGF-β. Here we establish miR-181a as a TGF-β-regulated "metastamir" that enhanced the metastatic potential of breast cancers by promoting epithelial-mesenchymal transition, migratory, and invasive phenotypes. Mechanistically, inactivation of miR-181a elevated the expression of the proapoptotic molecule Bim, which sensitized metastatic cells to anoikis. Along these lines, miR-181a expression was essential in driving pulmonary micrometastatic outgrowth and enhancing the lethality of late-stage mammary tumors in mice. Finally, miR-181a expression was dramatically and selectively upregulated in metastatic breast tumors, particularly triple-negative breast cancers, and was highly predictive for decreased overall survival in human breast cancer patients. Collectively, our findings strongly implicate miR-181a as a predictive biomarker for breast cancer metastasis and patient survival, and consequently, as a potential therapeutic target in metastatic breast cancer.
Beauty in a box Thompson, Cheryl
Beauty in a box,
2019, 2019, 2018, 2019-04-17
eBook
One of the first transnational, feminist studies of Canada's black beauty culture and the role that media, retail, and consumers have played in its development, Beauty in a Box widens our ...understanding of the politics of black hair. The book analyzes advertisements and articles from media--newspapers, advertisements, television, and other sources--that focus on black communities in Halifax, Montreal, Toronto, and Calgary. The author explains the role local black community media has played in the promotion of African American-owned beauty products; how the segmentation of beauty culture (i.e., the sale of black beauty products on store shelves labelled "ethnic hair care") occurred in Canada; and how black beauty culture, which was generally seen as a small niche market before the 1970s, entered Canada's mainstream by way of department stores, drugstores, and big-box retailers. Beauty in a Box uses an interdisciplinary framework, engaging with African American history, critical race and cultural theory, consumer culture theory, media studies, diasporic art history, black feminism, visual culture, film studies, and political economy to explore the history of black beauty culture in both Canada and the United States.
Circulating exosomes provide a promising approach to assess novel and dynamic biomarkers in human disease, due to their stability, accessibility and representation of molecules from source cells. ...However, this potential has been stymied by lack of approaches for molecular profiling of individual exosomes, which have a diameter of 30-150 nm. Here we report a rapid analysis approach to evaluate heterogeneous surface protein expression in single circulating exosomes from human blood. Our studies show a differential CD47 expression in blood-derived individual circulating exosomes that is correlated with breast cancer status, demonstrating a great potential of individual exosome profiles in biomarker discovery. The sensitive and high throughput platform of single exosome analysis can also be applied to characterizing exosomes derived from other patient fluids.
Since improvements in cancer screening, diagnosis, and therapeutics, cancer disparities have existed. Marginalized populations (e.g., racial and ethnic minorities, sexual and gender minorities, ...lower-income individuals, those living in rural areas, and persons living with disabilities) have worse cancer-related outcomes. Early detection of cancer substantially improves outcomes, yet uptake of recommended cancer screenings varies widely. Multi-cancer early detection (MCED) tests use biomarkers in the blood to detect two or more cancers in a single assay. These assays show potential for population screening for some cancers-including those disproportionally affecting marginalized communities. MCEDs may also reduce access barriers to early detection, a primary factor in cancer-related outcome disparities. However, for the promise of MCEDs to be realized, during their development and testing, we are obligated to be cautious to design them in a way that reduces the myriad of structural, systematic, and personal barriers contributing to disparities. Further, they must not create new barriers. Population studies and clinical trials should include diverse populations, and tests must work equally well in all populations. The tests must be affordable. It is critical that we establish trust within marginalized communities, the healthcare system, and the MCED tests themselves. Tests should be expected to have high specificity, as a positive MCED finding will trigger additional, oftentimes invasive and expensive, imaging or other diagnosis tests and/or biopsies. Finally, there should be a way to help all individuals with a positive test to navigate the system for follow-up diagnostics and treatment, if warranted, that is accessible to all.
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