To investigate the effect of enoxaparin on ovarian reserve and serum antimüllerian hormone (AMH) levels in a rat ovarian torsion model.
Experimental study.
Experimental surgery laboratory in a ...training and research hospital.
Fourteen female Wistar Hannover rats.
1) Control group received no special treatment other than abdominal exposure; 2) detorsion-only group received bilateral adnexal torsion (3-hour ischemia), and then after 3-hour torsion period, detorsion (reperfusion) was performed; and 3) detorsion-enoxaparin group received 0.5 mg/kg enoxaparin subcutaneously 2 hours before the same surgery as the detorsion-only group and a second 0.5 mg/kg dose of enoxaparin 24 hours after the first surgeries. Apart from the surgeries, preoperative and postoperative 1-mL blood samples were drawn from the right jugular vein of each rat.
Preoperative and postoperative serum AMH levels, histopathologic damage scores, and follicle counts in the ovarian tissue of the rats.
Vascular congestion and hemorrhage scores were higher in the detorsion-enoxaparin group than in the detorsion-only and control groups. The number of small antral follicles was smaller in the detorsion-only group than in the control group. The difference in the pre- and postoperative AMH levels was higher in the detorsion-only group than in the control and detorsion-enoxaparin groups.
The combination of enoxaparin therapy with conventional ovarian detorsion is more effective in protecting the ovarian reserve than detorsion alone.
Objectives: This study aims to report the incidence of inadvertent parathyroid gland removal during thyroidectomies, as documented by pathology
reports, and investigate its association with ...hypocalcemia.
Materials and Methods: The patients who underwent thyroidectomy at our department between 2009 and 2013 were retrospectively reviewed. The
patients who had standard total thyroidectomy were included in the study. Data on patient demographics, surgical reports, experience of surgeons,
anatomical localization of excised parathyroid glands (PG) and laboratory results were evaluated. Patients were divided into two groups according
to the presence or absence of an excised PG in the histopathology report (group P: presence of PG, and group T: absence of PG).
Results: A total of 343 patients were included to study. Of these, 26 patients were in group P, and 317 in group T. Post-operative hypocalcemia was
encountered in 37 patients. Hypocalcemia rates were similar between two groups (p=0.45). Surgical experience was found to decrease the rate of
Inadvertent parathyroidectomy (IP) (p=0.04), however, it wasn’t associated with post-operative hypocalcemia (p=0.72).
Conclusion: IP is an undesirable finding that can be encountered by surgeons. Our findings showed that IP was found to be associated with surgical
experience. However, these results were not associated with post-operative hypocalcemia.
Abstract Background context Spinal extradural angiolipomas are rare benign tumors, and most of them are noninfiltrating tumors located in the extradural space. However, there are 17 cases with ...extradural infiltrating spinal angiolipomas extending into the vertebral bodies or posterior vertebral arches in literature. These 17 tumors are mostly located at the thoracic region, and anterior or in the anterolateral extradural space, and they generally infiltrate only one vertebra. Only two of them are located at the lumbar region, four are mainly located in the posterior extradural space, and three infiltrate more than one vertebra. Purpose To present an exceptional case with infiltrating extradural spinal angiolipoma involving two lumbar segments and mainly located in the posterior extradural space. Study design A case report. Methods A 41-year-old woman with infiltrating spinal angiolipoma was treated by incomplete surgical removal of the tumor. Results There were no complaints nor recurrence after 18 months follow-up. Conclusions Outcome after surgery for spinal angiolipomas is very good overall even in the cases with infiltrating tumors. Although complete removal is certainly preferred, outcomes remained favorable despite incomplete resections.
Abstract Objective To investigate the effects of tubal sterilization techniques on the ovarian reserve and serum anti-Müllerian hormone (AMH) levels in rats. Study design Wistar Hannover rats ( n = ...21) were assigned to the following 3 groups (7 rats/group): Pomeroy technique, bipolar electrocauterization, and control. Pre- and postoperative serum AMH levels, ovarian damage and ovarian follicle counts were assessed. Results Total ovarian damage was higher in the electrocauterization group than in the Pomeroy ( p = 0.008) and control ( p < 0.0001) groups. The large antral follicle count was higher in the Pomeroy group than in the electrocauterization ( p = 0.023) and control ( p = 0.003) groups. The electrocauterization group had a greater decrease in postoperative serum AMH levels than the Pomeroy and control groups ( p = 0.031). Conclusion Bipolar electrocauterization may have a negative effect on the ovarian structure and ovarian reserve compared to the Pomeroy technique.
To investigate the relationship between diffusion tensor imaging (DTI) parameters such as fractional anisotropy (FA), mean diffusivity (MD), relative anisotropy (RA), and volume ratio (VR) values, ...and prognostic factors of invasive breast cancer.
This retrospective study examined 63 patients with pathologically confirmed invasive breast cancers. The patients underwent pre-operative diffusion-weighted magnetic resonance imaging (MRI) at 3.0 Tesla. The relationship between DTI parameters and tumor size, histologic and nuclear grade, axillary lymph node status, lymphovascular and perineural invasion status, estrogen receptor (ER), progesterone receptor (PR), CERB-B2, and Ki-67 were analyzed.
Patients with lymph node metastasis (p = 0.018; p < 0.05) and/or lymphovascular invasion (p = 0.001; p < 0.01) and/or histologic grade 3 tumors (p < 0.05) had statistically significantly low MD values. There was a statistically significant relationship between ER and MD (r = 0.452, p < 0.01), PR and MD (p = 0.001, p < 0.01); CERB-B2 and RA (p = 0.047, p < 0.05); Ki-67 and RA (p = 0.026; p < 0.05); Ki-67 and VR (p = 0.021; p < 0.05); and lymphovascular invasion and FA (p = 0.045, p < 0.05) values.
DTI parameters of malignant masses in breast cancer patients correlate with tumor size, lymph node status, histologic grade, lymphovascular invasion, Ki-67, CERB B2, ER, and PR.
•Diffusion tensor imaging (DTI) can provide valuable information on tissue microstructure and pathophysiology.•Malignant tumors with high cellularity exhibit low ADC values due to their inverse relationship with tumor cellularity.•DTI parameters of breast cancer tumors correlate with tumor size, lymph node status, histologic grade, lymphovascular invasion, Ki-67, CERB B2, ER, and PR
Abstract Objective We assess follicular reserve changes by follicle count in torsion–detorsion rat model. Study design 30 albino rats were randomly divided into 3 groups: sham group (SG), detorsion ...after 24-hour torsion group (24hTG) and detorsion after 72-hour torsion group (72hTG). Ovaries were torsioned and fixed. They were untwisted 24 and 72 h later. Oophorectomies were performed at 14th day after detorsion. Tissue damage scoring and follicle counts were evaluated microscopically. Results Tissue damage scores (TDSs) were higher in 72hTG and 24hTG compared to SG. In addition, as we increase torsion duration, TDSs also increased. There was no statistically significant difference in follicle numbers (primordial, primary, secondary and tertiary). Conclusion Duration of torsion and intensity of ovarian damage do not affect follicular reserve in a rat model. Regardless of their macroscopic appearance, ovaries maintain their follicle reserves after torsion. Thus, surgeons should be reassured and encouraged to untwist torsioned ovaries rather than removing them.
We aimed to evaluate the role of a synthetic somatostatin analogue in delay procedure of experimental skin flaps. Thirty-six rats were randomly divided into 2 groups of 18 each to compare the ...possible local ischemic effect of octreotide with that of surgical delay in the dorsal random pattern skin flap model. The inducible nitric oxide synthase gene expression was assessed in the flap territory at intervals of immediate, 24 and 48 hours after preconditioning. Histologic analysis was performed in rats at 48th hour and 3 additional rats were used for microangiography. A gradual increase of daily transcript levels was detected in both groups (P < 0.05). The differences of molecular and histologic findings between the groups were not distinctive. Pharmacologically preconditioned rat displayed relevant microvascular features. Forty rats were further grouped randomly into 4 groups of 10 each. In group 1 rats, flaps were raised and reinserted without any prior intervention. Group 2 rats underwent surgical delay procedure, whereas flap territories of the others received either saline solution or octreotide 1 week before the ultimate flap harvest. After another 7-day period, both delay procedures were found effective in improving flap viability (P < 0.01). Ischemia induced by octreotide favored to investigate its utility in delay phenomenon. Although it was not as effective as the surgical delay procedure, it may be a safe pharmacologic alternative to improve the flap survival.
Juvenile hyaline fibromatosis (JHF) is a rare systemic disease characterized by papulonodular skin lesions, gingival hyperplasia, joint contractures, and osteolytic lesions on long bones and the ...skull. It has recently been reported that the disease is caused by mutations in the gene encoding capillary morphogenesis protein-2 (CMG-2). To date, fewer than 60 cases have been published in the literature. Partial disease expression is common, but no cases featuring a solitary calvarial lesion have been reported. The authors discuss this 4-year-old boy with a solitary calvarial osteolytic lesion whose histopathological examination exhibited findings characteristic of JHF. Mutational analysis, however, revealed that there were no mutations in the CMG-2 gene. Two years after surgery, he was free of any complaints as well as gingival hyperplasia, joint contractures, and new skull or skin lesions. This patient's condition may represent clinical or genetic heterogeneity associated with JHF. Whether solitary lesions mimicking JHF can arise from somatic mutation of the CMG-2 gene remains to be proven.
Castleman disease (CD) is a benign lymphoepithelial disorder characterised by abnormal growth of a lymph node. This disease may be seen anywhere along the lymphatic chain but it usually occurs in the ...mediastinum. Two clinical types (localised and generalised) and three histopathological types (hyaline vascular, plasma cell, and mixed) have been described. We present a case of a 35-year-old female patient with parotid CD of the hyaline-vascular type as an extremely rare entity. We also discuss recent updates on this disease.
PDF
