Forming Limit Curve (FLC) is a well-known tool for the evaluation of failure in sheet metal process. However, its experimental determination and evaluation are rather complex. From theoretical point ...of view, FLC describes initiation of the instability not fracture. During the last years Digital Image Correlation (DIC) techniques have been developed extensively. Throughout this paper, all the measurements were done using DIC and as it is reported in the literature, different approaches to capture necking and fracture phenomena using Cross Section Method (CSM), Time dependent Method (TDM) and Thinning Method (TM) were investigated. Each aforementioned method has some advantages and disadvantages. Moreover, a cruciform specimen was used in order to cover whole FLC in the range between uniaxial to equi-biaxial tension and as an alternative for Nakajima test. Based on above-mentioned uncertainty about the fracture strain, some advanced numerical failure models can describe necking and fracture phenomena accurately with consideration of anisotropic effects. It is noticeable that in this paper, dog-bone, notch and circular disk specimens are used to calibrate Johnson-Cook (J-C) fracture model. The results are discussed for mild steel DC01.
The most common complications related to the treatment of childhood acute lymphoblastic leukemia (ALL) are infections. The aim of the study was to analyze the incidence and mortality rates among ...pediatric patients with ALL who were treated in 17 Polish pediatric hematology centers in 2020-2021 during the pandemic. Additionally, we compared these results with those of our previous study, which we conducted in the years 2012-2017. The retrospective analysis included 460 patients aged 1-18 years with newly diagnosed ALL. In our study, 361/460 (78.5%) children were reported to have microbiologically documented bacterial infections during chemotherapy. Ten patients (2.8%) died due to sepsis. Fungal infections were reported in 99 children (21.5%), of whom five (5.1%) died due to the infection. We especially observed an increase in bacterial infections during the pandemic period compared to the previous study. The directions of our actions should be to consider antibiotic prophylaxis, shorten the duration of hospitalization, and educate parents and medical staff about complications (mainly infections) during anticancer therapy. It is necessary to continue clinical studies evaluating infection prophylaxis to improve outcomes in childhood ALL patients.
The open science movement has gained significant momentum within the last few years. This comes along with the need to store and share research artefacts, such as publications and research data. For ...this purpose, research repositories need to be established. A variety of solutions exist for implementing such repositories, covering diverse features, ranging from custom depositing workflows to social media-like functions.
In this article, we introduce the FAIREST principles, a framework inspired by the well-known FAIR principles, but designed to provide a set of metrics for assessing and selecting solutions for creating digital repositories for research artefacts. The goal is to support decision makers in choosing such a solution when planning for a repository, especially at an institutional level. The metrics included are therefore based on two pillars: (1) an analysis of established features and functionalities, drawn from existing dedicated, general purpose and commonly used solutions, and (2) a literature review on general requirements for digital repositories for research artefacts and related systems. We further describe an assessment of 11 widespread solutions, with the goal to provide an overview of the current landscape of research data repository solutions, identifying gaps and research challenges to be addressed.
Mycotoxins are present in everyday diet as common food and feed pollutants. A part of them is still concerned as so-called emerging mycotoxins. Due to the lack of toxicity data, the safety limits and ...detail molecular mechanism have been not established yet for all of them. Alternariol (AOH), as one of these mycotoxins, produced by
species, is so far reported as an estrogenic, genotoxic, and immunomodulatory agent; however, its direct effect on human health is not known. Especially, in the case of hormone-dependent tissues which are sensitive to both endogenic, as well as external estrogenic agents, it might be crucial to assess the effect of AOH. Thus, this study evaluated how exposure to AOH affects viability and motility of the human normal mammary gland epithelial in vitro model. We observed that AOH significantly affects viability of cells in a time- and dose-dependent manner. Moreover, the induction of oxidative stress, DNA damage, and cell cycle arrest in the G2/M cell cycle phase was observed. The motility of 184A1 cells was also significantly affected. On the molecular level, AOH induced antioxidative stress response via activation of Nuclear factor erythroid 2-related factor 2 (NRF2) signaling pathway agents, as well as decrease in the phosphorylation of protein kinase B (Akt) and p44/42 (ERK 1-2) molecules, indicating that AOH might affect crucial signaling pathways in both physiological and pathophysiological processes in breast tissue.
The 2016 World Thrombosis Day in Poland Zawilska, Krystyna; Urbanek, Tomasz
Polskie Archiwum Medycyny Wewnętrznej,
2016-Dec-23, Letnik:
126, Številka:
12
Journal Article
•The paper describes a technique for detection of modulations in vibroacoustic signals.•Detection of rolling elements bearing faults indicators in high noise environment.•The problem of practical ...implementation of proposed tool in the industrial condition monitoring system.
Modulations present in vibration signals generated by rotating machinery might carry a lot of useful information about objects’ technical condition. It has been proven that both gearboxes and rolling element bearing (REB) faults manifest themselves as modulations. The paper describes a technique for detection of modulations in vibroacoustic signals, called modulation intensity distribution (MID), which is a function that combines multiple spectral correlation densities in one way or another, depending on the application. Additionally, the paper describes a functional obtained by integrating an MID (denoted by IMID) that has the advantage of being a function of only one frequency variable instead of two. The paper investigates the utility of the MID as an indicator for detection of the presence of rolling element bearing faults in high noise environments. For the purpose of testing, a wind turbine that suffered both advanced gearbox fault and early stage of bearing fault was chosen. Additionally, the paper undertakes the problem of application of the proposed tool in an industrial condition-monitoring system. In order to show the behavior of cyclic components generated by the turbine under study over a long period of time, the set of MIDs integrated over full range of potential carrier signals was presented as a cascade plot.
Abstract
Background
Doxorubicin (DOX) is an effective anticancer drug, but its application is hampered by cardiotoxicity with asymptomatic diastolic dysfunction as the earliest manifestation. ...Interstitial fibrosis leads to impaired relaxation, but the timing of fibrotic process and the mechanisms that operate shortly after DOX exposure are not clear. While changes in energy metabolism by DOX is recognized in cardiomyocytes, the effects of DOX on cardiac fibroblasts (CFs) metabolism are unknown.Thus, in a model of anthracycline cardiomyopathy, we asked whether the activation of CFs anticipates the onset of DOX-induced early diastolic dysfunction and evaluated the effects of DOX on the activation and metabolism of CFs.
Methods
Fischer 344 rats were exposed to 6 injections of 2.5 mg/kg of DOX over 2 weeks. Heart function was assessed by echocardiography and left ventricular catheterization. To test the very early in vivo effects of DOX on CFs, primary CFs were isolated from hearts of DOX-treated rats after the first injection of DOX. In another set of experiments, CFs were exposed to DOX in vitro. Cell metabolism was measured by a Seahorse-XF Real-Time ATP assay and cell phenotype was determined by immunocytochemistry and molecular biology.
Results
Early effects of DOX consisted of unchanged ejection fraction and the evidence of diastolic dysfunction. Markers of pro-fibrotic remodeling and histological evidence of CFs transformation were present in the heart immediately after treatment completion. Importantly, at the very beginning of the DOXO exposure, when cardiac function was normal and there was no histological evidence of increased fibrosis, the conversion of CFs to myofibroblasts, evidenced by SMA, was detected. Oxygen consumption rate and extracellular acidification, revealed the increased metabolic activity of CFs after DOX exposure and the switch to the glycolytic energy production. These effects were consistent in CFs isolated from the hearts of DOX-treated animals and in naive CFs exposed to DOX in vitro. The metabolic switch was paralleled with the phenotype change of CFs towards extracellular matrix-producing cells. Upon DOX, CFs upregulated markers of myofibroblast differentiation and the activation of pro-fibrotic signaling (aSMA, TGFb and phospho-SMAD). CFs maintained their functional properties in scratch assay. CFs upregulated also NOX2, indicating these cells as an intramyocardial source of reactive oxygen in the initial phase of the disease.
Conclusion
The metabolic switch and activation of CFs anticipate the onset of DOX-induced early diastolic dysfunction. The effect of DOX on the metabolic profile in CFs represents a novel and potentially targetable component of the very early phase of the pathogenesis of anthracycline cardiomyopathy.
The presented study examines the question of colour categorization in relation to the hypothesis of linguistic relativity. The study is based on research conducted by Gilbert et al. (2006) and their ...claim that linguistic colour categorization in a particular language helps colour recognition and speeds the process of colour discrimination for colours from different linguistic categories but only for the right visual field. Our study approached the research question differently. We used the same methodology as Gilbert’s team et al. (2006), but we used different colour categories in the Czech language and significantly enlarged the number of participants to 106 undergraduate psychology students. Our results show that the fastest reaction times were in trials when the target was located in the left visual field, quite opposite from the Gilbert’s et al. (2006) study. We argue that this finding is based on different processes than simple colour linguistic categorisation and attentional processes actually play an important role in the task.
Wrist-worn accelerometry metrics are not well defined in older adults. Accelerometry data from 720 participants (mean age 70 years, 55% women) were summarized into (a) total activity counts per day, ...(b) active minutes per day, (c) active bouts per day, and (d) activity fragmentation (the reciprocal of the mean active bout length). Linear regression and mixed-effects models were utilized to estimate associations between age and gait speed with wrist accelerometry. Activity counts per day, daily active minutes per day, and active bouts per day were negatively associated with age among all participants, while positive associations with activity fragmentation were only observed among those ≥65 years. More activity counts, more daily active minutes, and lower activity fragmentation were associated with faster gait speed. There were baseline age interactions with annual changes in total activity counts per day, active minutes per day, and activity fragmentation (Baseline age × Time, p < .01 for all). These results help define and characterize changes in wrist-based physical activity patterns among older adults.
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