Foodomics evaluation of bioactive compounds in foods Valdés, Alberto; Cifuentes, Alejandro; León, Carlos
TrAC, Trends in analytical chemistry (Regular ed.),
November 2017, 2017-11-00, Letnik:
96
Journal Article
Recenzirano
Foodomics involves the study of food and nutrition domains through the application and integration of advanced omics technologies, such as transcriptomics, proteomics and metabolomics. This ...integration, together with biostatistics, chemometrics and bioinformatics tools allows the evaluation of complex biological systems, like those involved in the underlying mechanisms of bioactive food compounds that help to interpret the association between dietary exposure and health. In this review, we present a critical discussion of the recent developments in Foodomics approaches (including metabolomics, proteomics and transcriptomics) to understand the bioactivity of food and food ingredients on health as well as for the discovery of novel biomarkers related to such activity.
•Advanced analytical techniques to study the relationship between food and health.•Omics approaches to investigate the molecular mechanisms of bioactive components.•Foodomics strategies to evaluate the health effects of food and food components.
SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is the coronavirus strain causing the respiratory pandemic COVID-19 (coronavirus disease 2019). To understand the pathobiology of ...SARS-CoV-2 in humans it is necessary to unravel the metabolic changes that are produced in the individuals once the infection has taken place. The goal of this work is to provide new information about the altered biomolecule profile and with that the altered biological pathways of patients in different clinical situations due to SARS-CoV-2 infection. This is done via metabolomics using HPLC-QTOF-MS analysis of plasma samples at COVID-diagnose from a total of 145 adult patients, divided into different clinical stages based on their subsequent clinical outcome (25 negative controls (non-COVID); 28 positive patients with asymptomatic disease not requiring hospitalization; 27 positive patients with mild disease defined by a total time in hospital lower than 10 days; 36 positive patients with severe disease defined by a total time in hospital over 20 days and/or admission at the ICU; and 29 positive patients with fatal outcome or deceased). Moreover, follow up samples between 2 and 3 months after hospital discharge were also obtained from the hospitalized patients with mild prognosis. The final goal of this work is to provide biomarkers that can help to better understand how the COVID-19 illness evolves and to predict how a patient could progress based on the metabolites profile of plasma obtained at an early stage of the infection. In the present work, several metabolites were found as potential biomarkers to distinguish between the end-stage and the early-stage (or non-COVID) disease groups. These metabolites are mainly involved in the metabolism of carnitines, ketone bodies, fatty acids, lysophosphatidylcholines/phosphatidylcholines, tryptophan, bile acids and purines, but also omeprazole. In addition, the levels of several of these metabolites decreased to "normal" values at hospital discharge, suggesting some of them as early prognosis biomarkers in COVID-19 at diagnose.
Reduction of food waste provides important environmental and economic benefits. Valorization of food by-products into edible materials is one of the most interesting strategies in this field. ...However, food by-products and wastes can contain chemical contaminants or potential pathogens that may endanger consumers’ health. Therefore, assuring quality and safety of these by-products is of utmost importance to take advantage of this valorization strategy.
In this review, safety evaluation of valorized by-products intended for human and animal consumption has been revised and critically discussed. With this aim, the most relevant applications of valorized by-products intended for livestock feed or food, nutraceutical, and pharmaceutical industries, in which quality and safety were assessed have been compiled. Moreover, the most common strategies for the analysis and removal of undesirable substances in these valorized by-products have been pointed out, as well as the requirements established by current regulations.
Despite the great number of applications found on food by-products valorization, only in a reduced number of works safety evaluation studies, such as physicochemical and microbiological assessments or the determination of toxic contaminants, were carried out. Among them, the development of decontamination procedures and processing approaches that avoid the generation of such undesirable substances, as well as effective analysis methods have been reported. However, in most cases the evaluation of results has been very complex and difficult due to the lack of specific legislation that regulates the suitability and safety of the new products to guarantee consumers’ safety.
•By-products valorization presents important economic and environmental benefits.•Food by-products require safety assessment for their use in humans or animals.•Safety evaluation of valorized by-products has been scarce up to date.•Physicochemical, microbiological and toxicological studies are discussed.•There is a lack of specific regulations to assure safety of valorized by-products.
Viral infections cause large problems in the world and deeper understanding of the disease mechanisms is needed. Here we present an analytical strategy to investigate the host cell protein changes ...during human adenovirus type 2 (HAdV-C2 or Ad2) infection of lung fibroblasts by stable isotope labelling of amino acids in cell culture (SILAC) and nanoLC-MS/MS. This work focuses on early phase of infection (6 and 12 h post-infection (hpi)) but the data is combined with previously published late phase (24 and 36 hpi) proteomics data to produce a time series covering the complete infection. As many as 2169 proteins were quantitatively monitored from 6 to 36 hpi, while some proteins were time-specific. After applying different filter criteria, 2027 and 2150 proteins were quantified at 6 and 12 hpi and among them, 431 and 544 were significantly altered at the two time points. Pathway analysis showed that the De novo purine and pyrimidine biosynthesis, Glycolysis and Cytoskeletal regulation by Rho GTPase pathways were activated early during infection while inactivation of the Integrin signalling pathway started between 6 and 12 hpi. Moreover, upstream regulator analysis predicted MYC to be activated with time of infection and protein and RNA data for genes controlled by this transcription factor showed good correlation, which validated the use of protein data for this prediction. Among the identified phosphorylation sites, a group related to glycolysis and cytoskeletal reorganization were up-regulated during infection. The results show specific aspects on how the host cell proteins, the final products in the genetic information flow, are influenced by Ad2 infection, which would be overlooked if only knowledge derived from mRNA data is considered.
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•More than 450 natural compounds have been identified in orange by-products extract.•PLE100 extract reduced β-amyloid proteotoxicity in CL4176 Caenorhabditis elegans.•294 genes are ...differentially expressed after PLE100 extract treatment.•Out of 818 identified intracellular metabolites, 54 were significantly altered.•Neuroprotective mechanisms related to the lipid metabolism and the stress response.
Citrus sinensis by-products are a promising source of neuroprotective molecules. In this study, a pressurized liquid extract of Citrus by-products (PLE100) has been extensively characterized, and its neuroprotective capacity tested in the Caenorhabditis elegans strain CL4176, a validated in vivo model of Alzheimer’s disease (AD). More than 450 compounds have been annotated in the extract, being triacylglycerols (TGs), stigmastanes, fatty acids (FAs) and carbohydrates the most abundant. The results demonstrate that worms PLE100-treated are significantly protected in a dose-dependent manner against the Aβ-peptide paralysis toxicity. The RNA-Seq data showed an alteration of 294 genes mainly related to the stress response defense along with genes involved in the lipid transport and metabolism. Moreover, the comprehensive metabolomics study allowed the identification of 818 intracellular metabolites, of which 54 were significantly altered (mainly lipids). The integration of these and previous results provides with new evidences of the protection mechanisms of this promising extract.
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•A urea-based buffer extracts more proteins than conventional Tris-HCl methods.•SCG from espresso machines have more proteins than SCG from other coffeemakers.•Medium/Dark roasted SCG ...protein hydrolysates have in vitro ACE-inhibitory activity.•Peptide identification in SCG protein hydrolysates by RP-HPLC-ESI-Q-TOF technology.
Several works have been focused on the extraction of polysaccharides, polyphenols and caffeine from spent coffee grounds (SCG) and their application in food formulations, but the peptide bioactivity from SCG protein hydrolysates has never been addressed. In the present work and for the first time, two different methods to isolate proteins from SCG have been compared, demonstrating that a urea-based extraction buffer provides a higher yield. This extraction method was then applied to compare the protein content in SCG from different coffee-brewing preparations, showing a higher protein content in SCG from espresso coffee machines. In addition, a polyphenol extraction step to remove interferences has been evaluated and the hydrolysis of the extracted proteins using alcalase and thermolysin enzymes has been compared. The effect of roasting degree on the antioxidant and in vitro angiotensin-converting enzyme (ACE)-inhibitory activity has been evaluated. The results show that the ACE-inhibitory activity is higher when SCG proteins are obtained from medium and dark roasted coffees and then hydrolyzed with thermolysin. Finally, the peptides contained in these hydrolysates have been identified by reversed-phase high-performance liquid chromatography coupled via electrospray ionization to a quadrupole time-of-flight mass spectrometer (RP-HPLC-ESI-Q-TOF).
Nonislet-cell tumor hypoglycemia (NICTH) is a rare paraneoplastic phenomenon well described in dogs and humans. Tumors associated with NICTH secrete incompletely processed forms of insulin-like ...growth factor-II (IGF-II), commonly named big IGF-II. These forms have increased bioavailability and interact with the insulin and IGF-I receptor causing hypoglycemia and growth-promoting effects. Immunoassays designed for human samples have been used to measure canine IGF-I and -II, but they possess some limitations. In addition, there are no validated methods for measurement of big IGF-II in dogs. In the present study, a targeted parallel reaction monitoring MS-based method previously developed for cats has been optimized and applied to simultaneously quantify the serum levels of IGF-I, IGF-II, and IGFBP-3, and for the first time, the levels of big IGF-II in dogs. This method allows the absolute quantification of IGF proteins using a mixture of QPrEST proteins previously designed for humans. The method possesses good linearity and repeatability and has been used to evaluate the IGF-system in a dog with NICTH syndrome. In this dog, the levels of big IGF-II decreased by 80% and the levels of IGF-I and IGFBP-3 increased approximately 20- and 4-times, respectively, after removal of the tumor.
In this work, a proteomics strategy based on nanoliquid chromatography–tandem mass spectrometry (nano-LC–MS/MS) using an Orbitrap high-resolution mass spectrometer together with stable isotope ...dimethyl labeling (DML) is applied to quantitatively examine relative changes in the protein fraction of HT-29 human colon cancer cells treated with different concentrations of a polyphenol-enriched rosemary extract over the time. The major objective of this study was to gain insights into the antiproliferative mechanisms induced by rosemary polyphenols. Using this methodology, 1909 and 698 proteins were identified and quantified in cell extracts. The polyphenol-enriched rosemary extract treatment changed the expression of several proteins in a time- and concentration-dependent manner. Most of the altered proteins are implicated in the activation of Nrf2 transcription factor and the unfolded protein response. In conclusion, rosemary polyphenols induced proteomic changes that were related to the attenuation of aggresome formation and activation of autophagy to alleviate cellular stress.
Agrifood by-products and microalgae represent a low-cost and valuable source of bioactive compounds with neuroprotective properties. However, the neuroprotective effectiveness of therapeutic ...molecules can be limited by their capacity to cross the blood-brain barrier (BBB) and reach the brain. In this research, various green extracts from
(ASFE),
(T33),
(PPC1),
., (OL-SS),
(PLE100) by-products and from the microalgae
(DS) that have demonstrated in vitro neuroprotective potential were submitted to an in vitro BBB permeability and transport assay based on an immortalized human brain microvascular endothelial cells (HBMEC) model. Toxicity and BBB integrity tests were performed, and the transport of target bioactive molecules across the BBB were evaluated after 2 and 4 h of incubation using gas and liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (GC/LC-Q-TOF-MS). The HBMEC-BBB transport assay revealed a high permeability of representative neuroprotective compounds, such as mono- and sesquiterpenoids, phytosterols and some phenolic compounds. The obtained results from the proposed in vitro BBB cellular model provide further evidence of the neuroprotective potential of the target natural extracts, which represent a promising source of functional ingredients to be transferred into food supplements, food additives, or nutraceuticals with scientifically supported neuroprotective claims.
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