We present spot properties on 32 periodic young stellar objects in IC 5070. Long term, \(\sim\)5 yr, light curves in the \(V\), \(R\), and \(I\)-bands are obtained through the HOYS (Hunting ...Outbursting Young Stars) citizen science project. These are dissected into six months long slices, with 3 months oversampling, to measure 234 sets of amplitudes in all filters. We fit 180 of these with reliable spot solutions. Two thirds of spot solutions are cold spots, the lowest is 2150 K below the stellar temperature. One third are warm spots that are above the stellar temperature by less than \(\sim\)2000 K. Cold and warm spots have maximum surface coverage values of 40 percent, although only 16 percent of warm spots are above 20 percent surface coverage as opposed to 60 percent of the cold spots. Warm spots are most likely caused by a combination of plages and low density accretion columns, most common on objects without inner disc excess emission in \(K-W2\). Five small hot spot solutions have \(<3\) percent coverage and are 3000 - 5000 K above the stellar temperature. These are attributed to accretion, and four of them occur on the same object. The majority of our objects are likely to be accreting. However, we observe very few accretion hot spots as either the accretion is not stable on our timescale or the photometry is dominated by other features. We do not identify cyclical spot behaviour on the targets. We additionally identify and discuss a number of objects that have interesting amplitudes, phase changes, or spot properties.
This paper is one in a series reporting results from small telescope observations of variable young stars. Here, we study the repeating outbursts of three likely Be stars based on long-term optical, ...near-infrared, and mid-infrared photometry for all three objects, along with follow-up spectra for two of the three. The sources are characterised as rare, truly regularly outbursting Be stars. We interpret the photometric data within a framework for modelling light curve morphology, and find that the models correctly predict the burst shapes, including their larger amplitudes and later peaks towards longer wavelengths. We are thus able to infer the start and end times of mass loading into the circumstellar disks of these stars. The disk sizes are typically 3-6 times the areas of the central star. The disk temperatures are ~40%, and the disk luminosities are ~10% of those of the central Be star, respectively. The available spectroscopy is consistent with inside-out evolution of the disk. Higher excitation lines have larger velocity widths in their double-horned shaped emission profiles. Our observations and analysis support the decretion disk model for outbursting Be stars.
Studying rotational variability of young stars is enabling us to investigate a multitude of properties of young star-disk systems. We utilise high cadence, multi-wavelength optical time series data ...from the Hunting Outbursting Young Stars citizen science project to identify periodic variables in the Pelican Nebula (IC5070). A double blind study using nine different period-finding algorithms was conducted and a sample of 59 periodic variables was identified. We find that a combination of four period finding algorithms can achieve a completeness of 85% and a contamination of 30% in identifying periods in inhomogeneous data sets. The best performing methods are periodograms that rely on fitting a sine curve. Utilising GaiaEDR3 data, we have identified an unbiased sample of 40 periodic YSOs, without using any colour or magnitude selections. With a 98.9% probability we can exclude a homogeneous YSO period distribution. Instead we find a bi-modal distribution with peaks at three and eight days. The sample has a disk fraction of 50%, and its statistical properties are in agreement with other similarly aged YSOs populations. In particular, we confirm that the presence of the disk is linked to predominantly slow rotation and find a probability of 4.8\(\times\)10\(^{-3}\) that the observed relation between period and presence of a disk has occurred by chance. In our sample of periodic variables, we also find pulsating giants, an eclipsing binary, and potential YSOs in the foreground of IC5070.
Global biodiversity in freshwater and the oceans is declining at high rates. Reliable tools for assessing and monitoring aquatic biodiversity, especially for rare and secretive species, are important ...for efficient and timely management. Recent advances in DNA sequencing have provided a new tool for species detection from DNA present in the environment. In this study, we tested whether an environmental DNA (eDNA) metabarcoding approach, using water samples, can be used for addressing significant questions in ecology and conservation. Two key aquatic vertebrate groups were targeted: amphibians and bony fish. The reliability of this method was cautiously validated in silico, in vitro and in situ. When compared with traditional surveys or historical data, eDNA metabarcoding showed a much better detection probability overall. For amphibians, the detection probability with eDNA metabarcoding was 0.97 (CI = 0.90–0.99) vs. 0.58 (CI = 0.50–0.63) for traditional surveys. For fish, in 89% of the studied sites, the number of taxa detected using the eDNA metabarcoding approach was higher or identical to the number detected using traditional methods. We argue that the proposed DNA‐based approach has the potential to become the next‐generation tool for ecological studies and standardized biodiversity monitoring in a wide range of aquatic ecosystems.
ABSTRACT
Compact sources can cause scatter in the scaling relationships between the amplitude of the thermal Sunyaev–Zel’dovich Effect (tSZE) in galaxy clusters and cluster mass. Estimates of the ...importance of this scatter vary – largely due to limited data on sources in clusters at the frequencies at which tSZE cluster surveys operate. In this paper, we present 90 GHz compact source measurements from a sample of 30 clusters observed using the MUSTANG2 instrument on the Green Bank Telescope. We present simulations of how a source’s flux density, spectral index, and angular separation from the cluster’s centre affect the measured tSZE in clusters detected by the Atacama Cosmology Telescope (ACT). By comparing the MUSTANG2 measurements with these simulations we calibrate an empirical relationship between 1.4 GHz flux densities from radio surveys and source contamination in ACT tSZE measurements. We find 3 per cent of the ACT clusters have more than a 20 per cent decrease in Compton-y but another 3 per cent have a 10 per cent increase in the Compton-y due to the matched filters used to find clusters. As sources affect the measured tSZE signal and hence the likelihood that a cluster will be detected, testing the level of source contamination in the tSZE signal using a tSZE-selected catalogue is inherently biased. We confirm this by comparing the ACT tSZE catalogue with optically and X-ray-selected cluster catalogues. There is a strong case for a large, high-resolution survey of clusters to better characterize their source population.
The place of tonsillectomy in the management of sore throat in adults remains uncertain.
To establish the clinical effectiveness and cost-effectiveness of tonsillectomy, compared with conservative ...management, for tonsillitis in adults, and to evaluate the impact of alternative sore throat patient pathways.
This was a multicentre, randomised controlled trial comparing tonsillectomy with conservative management. The trial included a qualitative process evaluation and an economic evaluation.
The study took place at 27 NHS secondary care hospitals in Great Britain.
A total of 453 eligible participants with recurrent sore throats were recruited to the main trial.
Patients were randomised on a 1 : 1 basis between tonsil dissection and conservative management (i.e. deferred surgery) using a variable block-stratified design, stratified by (1) centre and (2) severity.
The primary outcome measure was the total number of sore throat days over 24 months following randomisation. The secondary outcome measures were the number of sore throat episodes and five characteristics from Sore Throat Alert Return, describing severity of the sore throat, use of medications, time away from usual activities and the Short Form questionnaire-12 items. Additional secondary outcomes were the Tonsil Outcome Inventory-14 total and subscales and Short Form questionnaire-12 items 6 monthly. Evaluation of the impact of alternative sore throat patient pathways by observation and statistical modelling of outcomes against baseline severity, as assessed by Tonsil Outcome Inventory-14 score at recruitment. The incremental cost per sore throat day avoided, the incremental cost per quality-adjusted life-year gained based on responses to the Short Form questionnaire-12 items and the incremental net benefit based on costs and responses to a contingent valuation exercise. A qualitative process evaluation examined acceptability of trial processes and ramdomised arms.
There was a median of 27 (interquartile range 12-52) sore throats over the 24-month follow-up. A smaller number of sore throats was reported in the tonsillectomy arm median 23 (interquartile range 11-46) than in the conservative management arm median 30 (interquartile range 14-65). On an intention-to-treat basis, there were fewer sore throats in the tonsillectomy arm (incident rate ratio 0.53, 95% confidence interval 0.43 to 0.65). Sensitivity analyses confirmed this, as did the secondary outcomes. There were 52 episodes of post-operative haemorrhage reported in 231 participants undergoing tonsillectomy (22.5%). There were 47 re-admissions following tonsillectomy (20.3%), 35 relating to haemorrhage. On average, tonsillectomy was more costly and more effective in terms of both sore throat days avoided and quality-adjusted life-years gained. Tonsillectomy had a 100% probability of being considered cost-effective if the threshold for an additional quality-adjusted life year was £20,000. Tonsillectomy had a 69% probability of having a higher net benefit than conservative management. Trial processes were deemed to be acceptable. Patients who received surgery were unanimous in reporting to be happy to have received it.
The decliners who provided data tended to have higher Tonsillectomy Outcome Inventory-14 scores than those willing to be randomised implying that patients with a higher burden of tonsillitis symptoms may have declined entry into the trial.
The tonsillectomy arm had fewer sore throat days over 24 months than the conservative management arm, and had a high probability of being considered cost-effective over the ranges considered. Further work should focus on when tonsillectomy should be offered. National Trial of Tonsillectomy IN Adults has assessed the effectiveness of tonsillectomy when offered for the current UK threshold of disease burden. Further research is required to define the minimum disease burden at which tonsillectomy becomes clinically effective and cost-effective.
This trial is registered as ISRCTN55284102.
This award was funded by the National Institute for Health and Care Research (NIHR) Health Technology Assessment programme (NIHR award ref: 12/146/06) and is published in full in
; Vol. 27, No. 31. See the NIHR Funding and Awards website for further award information.
Tonsillectomy is regularly performed in adults with acute tonsillitis, but with scarce evidence. A reduction in tonsillectomies has coincided with an increase in acute adult hospitalisation for ...tonsillitis complications. We aimed to assess the clinical effectiveness and cost-effectiveness of conservative management versus tonsillectomy in patients with recurrent acute tonsillitis.
This pragmatic multicentre, open-label, randomised controlled trial was conducted in 27 hospitals in the UK. Participants were adults aged 16 years or older who were newly referred to secondary care otolaryngology clinics with recurrent acute tonsillitis. Patients were randomly assigned (1:1) to receive tonsillectomy or conservative management using random permuted blocks of variable length. Stratification by recruiting centre and baseline symptom severity was assessed using the Tonsil Outcome Inventory-14 score (categories defined as mild 0–35, moderate 36–48, or severe 49–70). Participants in the tonsillectomy group received elective surgery to dissect the palatine tonsils within 8 weeks after random assignment and those in the conservative management group received standard non-surgical care during 24 months. The primary outcome was the number of sore throat days collected during 24 months after random assignment, reported once per week with a text message. The primary analysis was done in the intention-to-treat (ITT) population. This study is registered with the ISRCTN registry, 55284102.
Between May 11, 2015, and April 30, 2018, 4165 participants with recurrent acute tonsillitis were assessed for eligibility and 3712 were excluded. 453 eligible participants were randomly assigned (233 in the immediate tonsillectomy group vs 220 in the conservative management group). 429 (95%) patients were included in the primary ITT analysis (224 vs 205). The median age of participants was 23 years (IQR 19–30), with 355 (78%) females and 97 (21%) males. Most participants were White (407 90%). Participants in the immediate tonsillectomy group had fewer days of sore throat during 24 months than those in the conservative management group (median 23 days IQR 11–46 vs 30 days 14–65). After adjustment for site and baseline severity, the incident rate ratio of total sore throat days in the immediate tonsillectomy group (n=224) compared with the conservative management group (n=205) was 0·53 (95% CI 0·43 to 0·65; <0·0001). 191 adverse events in 90 (39%) of 231 participants were deemed related to tonsillectomy. The most common adverse event was bleeding (54 events in 44 19% participants). No deaths occurred during the study.
Compared with conservative management, immediate tonsillectomy is clinically effective and cost-effective in adults with recurrent acute tonsillitis.
National Institute for Health Research.
Hollow fibre membrane contactor (HFMC) technology has been developed for CO2 absorption primarily using synthetic gas, which neglects the critical impact that trace contaminants might have on ...separation efficiency and robustness in industrial gases. This study, therefore, commissioned a demonstration-scale HFMC for CO2 separation at a full-scale anaerobic digester facility to evaluate membrane integrity over six months of operation on real biogas. The CO2 capture efficiency identified using real biogas was benchmarked at comparable conditions on synthetic gas of an equivalent partial pressure, and an equivalent performance identified. Two HFMC were subsequently compared, one with and one without a pre-treatment stage that targeted particulates, volatile organic compounds (VOCs) and humidity. Similar CO2 separation efficiency was again demonstrated, indicating limited impact within the timescale evaluated. However, gas phase pre-treatment is advised in order to ensure robustness in the long term. Over longer-term operation, a decline in CO2 separation efficiency was observed. Membrane autopsy identified shell-side deposition, where the structural morphology and confirmation of amide I and II groups, indicated biofouling. Separation efficiency was reinstated via chemical cleaning, which demonstrated that proactive maintenance could minimise process risk.
We designed and synthesized
, which is ∼21.6% shorter than native
, the smallest chromosome in
.
was designed for attachment to another synthetic chromosome due to concerns surrounding potential ...instability and karyotype imbalance and is now attached to
, yielding the first synthetic yeast fusion chromosome. Additional fusion chromosomes were constructed to study nuclear function.
and
fusion chromosomes have twisted structures, which depend on silencing protein Sir3. As a smaller chromosome,
also faces special challenges in assuring meiotic crossovers required for efficient homolog disjunction. Centromere deletions into fusion chromosomes revealed opposing effects of core centromeres and pericentromeres in modulating deposition of the crossover-promoting protein Red1. These effects extend over 100 kb and promote disproportionate Red1 enrichment, and thus crossover potential, on small chromosomes like
. These findings reveal the power of synthetic genomics to uncover new biology and deconvolute complex biological systems.
Methylmalonic aciduria (MMAuria) is an inborn error of metabolism leading to neurological deterioration. In this study, we used 3D organotypic brain cell cultures derived from embryos of a ...brain-specific Mut−/− (brain KO) mouse to investigate mechanisms leading to brain damage. We challenged our in vitro model by a catabolic stress (temperature shift).
Typical metabolites for MMAuria as well as a massive NH4+ increase were found in the media of brain KO cultures. We investigated different pathways of intracerebral NH4+ production and found increased expression of glutaminase 2 and diminished expression of GDH1 in Mut−/− aggregates. While all brain cell types appeared affected in their morphological development in Mut−/− aggregates, the most pronounced effects were observed on astrocytes showing swollen fibers and cell bodies. Inhibited axonal elongation and delayed myelination of oligodendrocytes were also noted. Most effects were even more pronounced after 48 h at 39 °C. Microglia activation and an increased apoptosis rate suggested degeneration of Mut−/− brain cells. NH4+ accumulation might be the trigger for all observed alterations. We also found a generalized increase of chemokine concentrations in Mut−/− culture media at an early developmental stage followed by a decrease at a later stage.
We proved for the first time that Mut−/− brain cells are indeed able to produce the characteristic metabolites of MMAuria. We confirmed significant NH4+ accumulation in culture media of Mut−/− aggregates, suggesting that intracellular NH4+ concentrations might even be higher, gave first clues on the mechanisms leading to NH4+ accumulation in Mut−/− brain cells, and showed the involvement of neuroinflammatory processes in the neuropathophysiology of MMAuria.
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