Nastava koja učenicima daje širok spektar mogućnosti za postizanje boljega razumijevanja nastavnih sadržaja i pristup nastavnom sadržaju sa širega aspekta jest integrirana nastava, a nastava glazbe ...pruža mnoštvo mogućnosti pri međupredmetnom povezivanju. U okviru rada provedeno je istraživanje na uzorku od 110 učitelja razredne nastave i učitelja predmetne nastave Glazbene kulture iz četiriju hrvatskih županija. Cilj istraživanja bio je utvrditi postoje li statistički značajne razlike u provođenju integrirane nastave Glazbene kulture i drugih predmeta između učitelja razredne nastave i učitelja Glazbene kulture, kao i razlike u njihovim mišljenjima o takvoj nastavi. Rezultati istraživanja upozorili su na potrebu intenzivnijega provođenja međupredmetne povezanosti nastave Glazbene kulture i drugih predmeta, koja doprinosi cjelovitosti, učinkovitosti i suvremenosti nastave u korist dobrobiti učenika.
Teaching that provides students with a wide range of opportunities to achieve a better understanding of curriculum content and an approach to curriculum content from a wider perspective is called integrated teaching, and teaching music offers many opportunities for cross-curricular integration. As part of the work, research was conducted on a sample of 110 classroom teachers and Music Culture subject teachers from four Croatian counties. The goal of the research was to determine whether there are statistically significant differences in the implementation of integrated teaching of Music Culture and other subjects between classroom teachers and Music Culture teachers, as well as differences in their opinions about such teaching. The results of the research pointed to the need for more intensive implementation of cross-curricular integration between the teaching of Music Culture and other subjects, which contributes to the completeness, efficiency, and modernity of the teaching for the benefit of the students.
High prevalence and mortality of prostate cancer (PCa) are well known global health issues. Novel biomarkers for better identifying patients with PCa are the subject of extensive research. Prostate ...specific antigen (PSA) shows low specificity in screening and diagnostics, leading to unnecessary biopsies and health costs. Eighty patients with PCa and benign prostate hyperplasia (BPH) were included in the study. We analyzed CAV1 gene expression and methylation in tissue. CAV1 cfDNA methylation from blood and seminal plasma was accessed as a potential PCa biomarker. Although methylation in blood plasma did not differ between PCa and BPH patients, methylation in seminal plasma showed better PCa biomarker performances than tPSA (AUC 0.63 vs. AUC 0.52). Discrimination of BPH and Gleason grade group 1 PCa patients from patients with higher Gleason grade groups revealed very good performance as well (AUC 0.72). CAV1 methylation is useful biomarker with potential for further seminal plasma cfDNA research, but its diagnostic accuracy should be improved, as well as general knowledge about cfDNA in seminal plasma.
A 31-year-old man with left-sided testicular pain lasting a couple of months was referred to our urology department due to a suspected testicular tumor. Physical examination showed a hard, thickened, ...and small left testis on palpation with a diffuse, inhomogeneous ultrasonographic appearance. After a urologic examination, a left-sided inguinal orchiectomy was performed. The testis, epididymis, and spermatic cord were sent to pathology. Gross examination revealed a cystic cavity filled with brown fluid and the surrounding brownish parenchyma measuring up to 3.5 cm in diameter. Histologic examination showed a cystically dilated rete testis lined with cuboidal epithelium and a positive immunohistochemical reaction to cytokeratins. Microscopically, the cystic cavity was a pseudocyst filled with extravasated erythrocytes and abundant clusters of siderophages. The siderophages extended into the testicular parenchyma, surrounding the seminiferous tubules and spreading out around the ducts of the epididymis, which were also cystically dilated with siderophages inside their lumina. On the basis of clinical data, histological, and immunohistochemical analysis, the patient was diagnosed with cystic dysplasia of the rete testis. The literature shows an association between cystic dysplasia of the rete testis and ipsilateral genitourinary anomalies. Therefore, our patient underwent a multi-slice computed tomography scan, which revealed ipsilateral renal agenesis, a right seminal vesicle cyst reaching up to the iliac arteries, and a multicystic formation cranial to the prostate.
The unmet challenge in prostate cancer (PCa) management is to discriminate it from benign prostate hyperplasia (BPH) due to the lack of specific diagnostic biomarkers. Contemporary research on ...potential PCa biomarkers is directed toward methylated cell-free DNA (cfDNA) from liquid biopsies since epigenetic mechanisms are strongly involved in PCa development.
In the present research, cfDNA methylation of the LGALS3 gene in blood and seminal plasma of PCa and BPH patients was assessed using pyrosequencing, as well as LGALS3 DNA methylation in tissue biopsies. Liquid biopsy samples were taken from patients with clinical suspicion of PCa, who were subsequently divided into two groups, that is, 42 with PCa and 55 with BPH, according to the histopathological analysis.
Statistically significant higher cfDNA methylation of LGALS3 in seminal plasma of BPH than in PCa patients was detected by pyrosequencing. ROC curve analysis showed that it could distinguish PCa and BPH patients with 56.4% sensitivity and 70.4% specificity, while PSA did not differ between the two patient groups. In contrast, there was no statistically significant difference in LGALS3 cfDNA methylation in blood plasma between the two patient groups. In prostate tumor tissue, there was a statistically significant DNA hypermethylation of LGALS3 compared to surrounding nontumor tissue and BPH tissue.
The DNA hypermethylation of the LGALS3 gene represents an event specific to PCa development. In conclusion, LGALS3 cfDNA methylation in seminal fluid discriminates early PCa and BPH presenting itself as a powerful novel PCa biomarker highly outperforming PSA.
