Three different LED spectra (W: White light; WFR: W + far-red light; WB: W + blue light) with similar photosynthetic photon flux density (PPFD) were designed to explore the effects of supplementary ...far-red and blue lights on leaf color, biomass and phytochemicals of two cultivars of red-leaf lettuce ("Yanzhi" and "Red Butter") in an artificial lighting plant factory. Lettuce plants under WB had redder leaf color and significantly higher contents of pigments, such as chlorophyll a, chlorophyll b, chlorophyll (a + b) and anthocyanins. The accumulation of health-promoting compounds, such as vitamin C, vitamin A, total phenolic compounds, total flavonoids and anthocyanins in the two lettuce cultivars were obviously enhanced by WB. Lettuce under WFR showed remarkable increase in fresh weight and dry weight; meanwhile, significant decreases of pigments, total phenolic compounds, total flavonoids and vitamin C were found. Thus, in the plant factory system, the application of WB can improve the coloration and quality of red leaf lettuce while WFR was encouraged for the purpose of elevating the yield of lettuce.
MicroRNAs (miRNA) play a vital role in insects’ growth and development and have significant potential value in pest control. Previously, we identified miR-306 from small RNA libraries within the ...English grain aphid, Sitobion avenae, a devasting insect pest for wheat. miR-306 not only involves in wing morphogenesis, but also is critically important for aphid survival. Its specific impacts on the life history traits, however, remain unclear. Here, we evaluate the impact of miR-306 perturbation on S. avenae populations using a two-sex life table approach. This comprehensive analysis revealed that miR-306 perturbation significantly prolongs the developmental stages (9.64% and 8.20%) and adult longevity of S. avenae, while decreasing pre-adult survival rate (41.45% and 38.74%) and slightly reducing average fecundity (5.80% and 13.05%). Overall, miR-306 perturbation negatively affects the life table parameters of the aphid population. The population prediction models show a significant decline in the aphid population 60 days post interference, compared to the control groups (98.14% and 97.76%). Our findings highlight the detrimental effects of miR-306 perturbation on S. avenae population growth and suggest potential candidate genes for the development of RNAi-based biopesticides targeted specifically at this pest species.
Compared with traditional rigid gripper with joint-linkage structure, novel soft robotic gripper gives rise to continuous concern for the advantages of no-damage grasping, convenient manufacture, ...easy control, and low cost. In this study, we design and built two kinds of soft robotic grippers with four fiber-reinforced soft actuators which are distributed in circular and rectangle shapes for single and twin contacts grasping. A novel hybrid valve pneumatic control scheme combining proportional and solenoid valves is proposed. Also, a mode controllable hybrid valve pressure control method is proposed to adjust internal pressure of soft robotic grippers to adapt to different grasping tasks. The experiment results verify that the performances of hybrid valve outperform those of individual proportional valve or solenoid valve in the aspects of response time and steady-state accuracy. The hybrid valve has wide range of pressure regulation, result in that the soft robotic grippers are qualified to grasp various objects with different shapes, sizes, and weights.
A novel variable stiffness soft robotic hand (SRH) consists of three pieces of layer jamming structure (LJS) is proposed. The mechanism is driven by the motor-based tendon along the surface of the ...pieces that connect to individual gas channel. Each LJS is optimised by adhering a thin layer of hot melt adhesive and overlapping the spring steel sheet as inner layer material. It can be switched between rigid and compliant independently. The structures of variable stiffness and tendon-driven lead to various deformation poses. Then the control system of SRH and the performance analysis of the LJS are introduced. Finally, the experiments are implemented to prove the superiority of the proposed LJS and the demonstrations show that the designed robotic hand has multiple configurations to successfully grasp various objects.
The investigation of genetics-based biopesticides has become a central focus in pesticide studies due to their inherent advantages, including species specificity, environmental safety, and a wide ...range of target genes. In this study, a mixture of miR-184 agomir and nanomaterial star polycation (SPc) was used to treat the nymphs of the English grain aphid, Sitobion avenae (F.). The life parameters of the aphids at various developmental stages were analyzed using an age–stage two-sex life table to assess the effect of miR-184 agomir on the experimental population. The results indicated that miR-184 agomir had a significant negative effect on four key life parameters, including the intrinsic rate of increase, the finite rate of increase, the net rate of increase, and the mean generation time. The population prediction revealed a substantial reduction (91.81% and 95.88%) in the population size of S. avenae at 60 d after treatment with miR-184 agomir, compared to the control groups. Our findings suggest that the miR-184 agomir has the potential to reduce the survival rate and mean longevity of S. avenae, highlighting its potential as a promising candidate for the development of an effective genetics-based biopesticide.
This paper focuses on how to improve the operation ability of a soft robotic hand (SRH). A trigger-based dexterous operation (TDO) strategy with multimodal sensors is proposed to perform autonomous ...choice operations. The multimodal sensors include optical-based fiber curvature sensor (OFCS), gas pressure sensor (GPS), capacitive pressure contact sensor (CPCS), and resistance pressure contact sensor (RPCS). The OFCS embedded in the soft finger and the GPS series connected in the gas channel are used to detect the curvature of the finger. The CPCS attached on the fingertip and the RPCS attached on the palm are employed to detect the touch force. The framework of TDO is divided into sensor detection and action operation. Hardware layer, information acquisition layer, and decision layer form the sensor detection module; action selection layer, actuator drive layer, and hardware layer constitute the action operation module. An autonomous choice decision unit is used to connect the sensor detecting module and action operation module. The experiment results reveal that the TDO algorithm is effective and feasible, and the actions of grasping plastic framework, pinching roller ball pen and screwdriver, and handshake are executed exactly.
Land use change plays an important role in regional socio-economic development and global environmental change. Whether the land is effectively and efficiently used is not only related to the income ...level of the people in the surrounding cities but also closely related to the local economy and national economy. Intelligent environment refers to the indoor environment with a variety of data acquisition equipment. Combined with related technologies, the reasoning and analysis of the data can be used to realize the functions of activity identification, data perception, and control. In addition, the Yangtze River Delta is an economically developed area in China, and its land use situation is related to the economic development in the next ten or even decades. Therefore, it is necessary to analyze the spatial and temporal pattern of land use in Yangtze River Delta region by remote sensing image technology and GIS in intelligent environment. Based on intelligent environment, this paper uses RS and GIS technology to interpret remote sensing image and map land use in multitemporal coastal zone. The land use dynamic degree model and spatial interpolation method were used to analyze and evaluate the spatial and temporal evolution characteristics of land use in the Yangtze River Delta region, and the landscape pattern changes in the Yangtze River Delta region were analyzed and evaluated. This study found that the land use types in the Yangtze River Delta have transformed each other, and the land use change speed is fast, which is inseparable from the rapid economic development. In the future, in addition to maintaining the rapid and stable development of industry, the rational use of limited land resources, the improvement of agricultural development short board, and the improvement of tourism economic benefits will make the economy of the Yangtze River Delta region to a new level.
In this paper, investigations on gallium indium nitride (GaInN) quantum well structures as optochemical transducers in (bio)chemical sensing are presented. In contrast to the conventional electrical ...read-out of III-nitride-based sensors, a purely optical photoluminescence read-out is performed. A significant spectral shift of the quantum well photoluminescence is observed with varying surface modification. The spectral photoluminescence shift can be attributed to an externally induced quantum confined Stark effect caused by the adsorbed species deposited on the quantum well surface. In order to improve the sensitivity of the transducer elements, different chemical surface treatments are studied. In particular, optical sensing experiments with reducing and oxidizing gases are performed in order to investigate the quantum well photoluminescence response. Additionally, optical investigations of the iron-storage molecule ferritin with varying iron load are presented. The iron load of this molecule is generally considered as a superior biomarker for severe illnesses, such as Alzheimer's disease. In contrast to conventional fluorescent labels, GaInN quantum wells provide a much more stable luminescence signal, and hence, are promising candidates for next generation bioanalytical sensor structures.
Four light treatments (W: white light; EOD-B: end-of-day enhanced blue light; EOD-FR: end-of-day supplementary far-red light; EOD-UV: end-of-day supplementary ultraviolet-A light) were designed to ...explore the effects of end-of-day (EOD) lightings (30 min before dark period) on leaf color, biomass and phytochemicals accumulation in two lettuce cultivars (Lactuca sativa cv. ‘Red butter’ and ‘Green butter’) in artificial light plant factory. EOD-FR stimulated the plant and shoot biomass of two cultivars, and EOD-B suppressed the growth of ‘Red butter’ but induced higher biomass in ‘Green butter’. EOD lightings generated brighter, greener and yellower leaf in ‘Red butter’ at harvest, but the highest lightness and the deepest redness of ‘Green butter’ leaf were observed in the middle growth stage. ‘Red butter’ had prominent higher contents of chlorophylls and carotenoids, while these pigments showed less sensitivity to the interaction of cultivars and EOD lightings. EOD lightings impeded the accumulation of anthocyanin in two cultivars, except EOD-UV slightly increased the anthocyanin contents in ‘Green butter’. EOD-UV strengthened the antioxidant capability of ‘Green butter’, but EOD lightings had different effects on the antioxidant and nutritional compound contents in two lettuce cultivars.
Intrinsically magnetic cells naturally occur within organisms and are believed to be linked to iron metabolism and certain cellular functions while the functional significance of this magnetism is ...largely unexplored. To better understand this property, an approach named Optical Tracking‐based Magnetic Sensor (OTMS) has been developed. This multi‐target tracking system is designed to measure the magnetic moment of individual cells. The OTMS generates a tunable magnetic field and induces movement in magnetic cells that are subsequently analyzed through a learning‐based tracking‐by‐detection system. The magnetic moment of numerous cells can be calculated simultaneously, thereby providing a quantitative tool to assess cellular magnetic properties within populations. Upon deploying the OTMS, a stable population of magnetic cells in human peripheral monocytes is discovered. Further application in the analysis of clinical blood samples reveals an intriguing pattern: the proportion of magnetic monocytes differs significantly between systemic lupus erythematosus (SLE) patients and healthy volunteers. This variation is positively correlated with disease activity, a trend not observed in patients with rheumatoid arthritis (RA). The study, therefore, presents a new frontier in the investigation of the magnetic characteristics of naturally occurring magnetic cells, opening the door to potential diagnostic and therapeutic applications that leverage cellular magnetism.
This study introduces a novel Optical Tracking‐based Magnetic Sensor (OTMS) for precisely measuring the magnetic moment of individual cells. By applying the OTMS, a distinct population of magnetic monocytes in humans is identified, whose percentage significantly differs between systemic lupus erythematosus (SLE) patients and healthy individuals. These findings suggest a potential correlation between magnetic monocytes with autoimmune diseases.
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